ABSTRACT
This work was aimed at comparative analysis of the two experimental models of chronic arthritis induced in rats by the administration of (i) complete Freund's adjuvant (CFA) and (ii) carrageenan. The clinical and histological signs of pathology were assessed using a categorical rating system. It is established that the administration of CFA leads to the development of pathology closely resembling rheumatoid arthritis. In contrast, the administration of c.arrageenan promotes chronic inflammatory arthritis without autoimmune component. The results can serve a basis for selecting a methodological approach to assess the effectiveness of drugs with anti-rheumatoid, anti-inflammatory, and/or immunomodulatory properties.
Subject(s)
Arthritis, Experimental , Animals , Arthritis, Experimental/chemically induced , Arthritis, Experimental/metabolism , Arthritis, Experimental/pathology , Arthritis, Experimental/physiopathology , Female , Rats , Rats, WistarABSTRACT
The aim of this work was to study the behavioral and histopathomorphological signs of peripheral neuropathy development in male Wistar rats on the model of alcoholic neuropathy. Chronic consumption of ethanol solution with concentration increasing from 7.47 to 26.2% (w/w) resulted in neuropathy (allodynia) de- velopment after 8 weeks of chronic alcohol administration. The behavioral signs of allodynia became significant on the 8th week and were retained up to the end of experiment (15 weeks of ethanol administration). The reference drug gabapentin effectively reduced the manifestation of allodinia. Histological exami- nation of sciatic nerve preparations from animals killed after ethanol consumption for 5, 10 and 15 weeks revealed the development of histopathomorphological pattern with increasing duration of chronic alcoholization. At the initial stage, the morphological basis of observed behavioral manifestations was provided by excess lipid deposition in peri/epineurium of nerve specimens). The further increase in treatment duration (up to 10 and 15 weeks) was associated with demye- lination and development of inflammation of the sciatic nerve. This experimental model allows one to investigate the efficacy of new neuroprotective and ana- lgesic substances - potential drugs for both prevention and management of neuropathy.
