Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 3 de 3
Filter
Add more filters










Database
Language
Publication year range
1.
Sci Rep ; 10(1): 7071, 2020 04 27.
Article in English | MEDLINE | ID: mdl-32341392

ABSTRACT

Root systems are dynamic and adaptable organs that play critical roles in plant development. However, how roots grow and accumulate biomass during plant life cycle and in relation to shoot growth phenology remains understudied. A comprehensive time-dependent root morphological analysis integrated with molecular signatures is then required to advance our understanding of root growth and development. Here we studied Brachypodium distachyon rooting process by monitoring root morphology, biomass production, and C/N ratios during developmental stages. To provide insight into gene regulation that accompanies root growth, we generated comprehensive transcript profiles of Brachypodium whole-root system at four developmental stages. Our data analysis revealed that multiple biological processes including trehalose metabolism and various families of transcription factors (TFs) were differentially expressed in root system during plant development. In particular, the AUX/IAA, ERFs, WRKY, NAC, and MADS TF family members were upregulated as plant entered the booting/heading stage, while ARFs and GRFs were downregulated suggesting these TF families as important factors involved in specific phases of rooting, and possibly in regulation of transition to plant reproductive stages. We identified several Brachypodium candidate root biomass-promoting genes and cis-regulatory elements for further functional validations and root growth improvements in grasses.


Subject(s)
Brachypodium/metabolism , Gene Expression Regulation, Plant/physiology , Plant Proteins/biosynthesis , Plant Roots/metabolism , Regulatory Sequences, Nucleic Acid/physiology , Brachypodium/genetics , Plant Proteins/genetics , Plant Roots/genetics
2.
J Mass Spectrom ; 55(4): e4363, 2020 Apr.
Article in English | MEDLINE | ID: mdl-31018010

ABSTRACT

The spatial configuration and morphology of roots are commonly monitored for a better understanding of plant health and development. However, this approach provides minimal details about the biochemistry regulating the observable traits. Therefore, the ability to metabolically map the entire root structure would be of major value. Here, we developed a sample preparation approach that enables imaging of the entire root within a restricted space (width of microscope slide), which was influenced by the Swiss-rolling technique. We were able to image and confidently identify molecules along the entire root structure from rolled-root tissue sections using multiple spatially resolved mass spectrometry approaches.


Subject(s)
Metabolome , Metabolomics/methods , Plant Roots/metabolism , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods , Brachypodium/metabolism
3.
Methods ; 184: 29-39, 2020 12 01.
Article in English | MEDLINE | ID: mdl-31655121

ABSTRACT

Sorghum [Sorghum bicolor (L.) Moench] is an important cereal crop noted for its ability to survive water-limiting conditions. Herein, we present an analytical workflow to explore the changes in histone modifications through plant developmental stages and two drought stresses in two sorghum genotypes that differ in their response to drought. Top-down mass spectrometry (MS) is an ideal method to profile histone modifications and distinguish closely related histone proteoforms. We analyzed leaves of 48 plants and identified 26 unique histone proteins and 677 unique histone proteoforms (124 full-length and 553 truncated proteoforms). We detected trimethylation on nearly all H2B N-termini where acetylation is commonly expected. In addition, an unexpected modification on H2A histones was assigned to N-pyruvic acid 2-iminylation based on its unique neutral loss of CO2. Interestingly, some of the truncated histones, in particular H4 and H3.2, showed significant changes that correlated with the growth and water conditions. The histone proteoforms could serve as targets in search of chromatin modifiers and ultimately have important ramifications in future attempts of studying plant epigenetic reprogramming under stress.


Subject(s)
Acclimatization/genetics , Histones/analysis , Mass Spectrometry/methods , Sorghum/physiology , Chromatography, Reverse-Phase/methods , Droughts , Epigenesis, Genetic , Gene Expression Regulation, Plant , Histone Code/genetics , Histones/genetics , Histones/metabolism , Plant Proteins/genetics , Protein Processing, Post-Translational , Pyruvic Acid/metabolism
SELECTION OF CITATIONS
SEARCH DETAIL
...