[Concentration of transferrin iron and transferrin saturation in blood]. / Kontsentratsiia zaliza transferynu i stupin' nasychennia transferynu, vyznacheni v tsil'nii krovi.

Transferrin iron, transferrin protein concentrations, and transferrin saturation have been determined for the first time in the whole blood. Microsamples were taken from healthy adults and patients with occupational secondary haemochromatosis using quantitative electron spin resonance technique. At elevated transferrin saturation, transferrin saturation values determined in the plasma and serum samples were shown to be less than respective values determined in the whole blood of the same patients. At increased transferrin iron concentration the difference between experimental and reference data sets determined in the blood and plasma was statistically significant in contrast to data sets determined in serum. Therefore, the analysis of the blood microsamples ensured an adequate estimation of transferrin iron concentration, especially at high transferrin saturation. A new index--transferrin iron concentration in the formed blood elements--was introduced. The values of the index were determined in the groups of healthy adults, patients with secondary occupational hemochromatosis and healthy newborns.

[Quantification of chelatable iron in biological tissue by electron spin spectroscopy]. / Vyznachennia v biologichnykh tkanynakh kontsentratsiï zaliza, shcho khelatuiet'sia, metodom spektroskopiï elektronnoho spinovoho rezonansu.

The present paper deals with the procedure of a quantitative determination of chelatable iron in biological microsamples (20-50 mg) using an electron spin resonance technique based on the iron incorporation into the stable iron (III)-desferrioxamine B complexes. The conclusion is, that the method developed is valuable one to characterize the availability of iron from different sources. Using the method developed, the share of iron outside the protein core was shown to be less then 0.5 percentages of the whole iron content in the horse spleen ferritin suspension with 100 mg protein per ml and average iron content of 810 iron atoms per protein molecule. For a set of tissue samples taken from internal organs and brain of mice, rats and rabbits the data on chelatable iron concentration have been obtained.