Cyanide Content of Cassava Food Products Available in Australia.

In 2009, Food Standards Australia New Zealand set a total cyanide content limit of 10 ppm for ready-to-eat cassava products to address food safety concerns about cyanogenic glucosides in cassava. This study surveys a range of cassava food products available in Melbourne, Australia, ten years after the implementation of these regulations. Of all the products tested, the mean cyanide content was greatest in ready-to-eat cassava chips (48.4 ppm), although imported ready-to-eat products had a higher mean cyanide content (95.9 ppm) than those manufactured in Australia (1.0 ppm). Cyanide was detected in frozen cassava products (grated mean = 12.9 ppm; whole root mean = 19.8 ppm), but was significantly reduced through processing according to packet instructions in both product types. Three methods were used to quantify total cyanide content: the evolved cyanide method, the picrate absorbance method and the picrate chart method, with satisfactory agreement between methods. The picrate absorbance and chart methods reported mean cyanide contents 13.7 ppm and 23.1 ppm higher, respectively, than the evolved cyanide method. Our results reaffirm the need for the ongoing testing of cassava food products, especially ready-to-eat products whose cyanide content will not be reduced before consumption.

Nitrogen availability and allocation in sorghum and its wild relatives: Divergent roles for cyanogenic glucosides.

Crop plants are assumed to have become more susceptible to pests as a result of selection for high growth rates during the process of domestication, consistent with resource allocation theories. We compared the investment by domesticated sorghum into cyanogenic glucosides, nitrogen-based specialised metabolites that break down to release hydrogen cyanide, with five wild relatives native to Australia. Plants were grown in pots in a greenhouse and supplied with low and high concentrations of nitrogen and monitored for 9 weeks. The concentrations of nitrate, total phenolics and silicon were also measured. Domesticated Sorghum bicolor had the highest leaf and root cyanogenic glucoside concentrations, and among the lowest nitrate and silicon concentrations under both treatments. Despite partitioning a much higher proportion of its stored nitrogen to cyanogenic glucosides than the wild species, S. bicolor's nitrogen productivity levels were among the highest. Most of the wild sorghums had higher concentrations of silicon and phenolics, which may provide an alternative defence system. Cyanogenic glucosides appear to be integral to S. bicolor's physiology, having roles in both growth and defence. Sorghum macrospermum displayed consistently low cyanogenic glucoside concentrations, high growth rates and high nitrogen productivity and represents a particularly attractive genetic resource for sorghum improvement.

Wild Sorghum as a Promising Resource for Crop Improvement.

Sorghum bicolor (L.) Moench is a multipurpose food crop which is ranked among the top five cereal crops in the world, and is used as a source of food, fodder, feed, and fuel. The genus Sorghum consists of 24 diverse species. Cultivated sorghum was derived from the wild progenitor S. bicolor subsp. verticilliflorum, which is commonly distributed in Africa. Archeological evidence has identified regions in Sudan, Ethiopia, and West Africa as centers of origin of sorghum, with evidence for more than one domestication event. The taxonomy of the genus is not fully resolved, with alternative classifications that should be resolved by further molecular analysis. Sorghum can withstand severe droughts which makes it suitable to grow in regions where other major crops cannot be grown. Wild relatives of many crops have played significant roles as genetic resources for crop improvement. Although there have been many studies of domesticated sorghum, few studies have reported on its wild relatives. In Sorghum, some species are widely distributed while others are very restricted. Of the 17 native sorghum species found in Australia, none have been cultivated. Isolation of these wild species from domesticated crops makes them a highly valuable system for studying the evolution of adaptive traits such as biotic and abiotic stress tolerance. The diversity of the genus Sorghum has probably arisen as a result of the extensive variability of the habitats over which they are distributed. The wild gene pool of sorghum may, therefore, harbor many useful genes for abiotic and biotic stress tolerance. While there are many examples of successful examples of introgression of novel alleles from the wild relatives of other species from Poaceae, such as rice, wheat, maize, and sugarcane, studies of introgression from wild sorghum are limited. An improved understanding of wild sorghums will better allow us to exploit this previously underutilized gene pool for the production of more resilient crops.