Linfoma óseo primario / Primary bone lymphoma

La afectación esquelética en los pacientes con linfoma no hodgkiniano (LNH) no es infrecuente. Suele ser una manifestación tardía, y cuando aparece lo hace generalmente de forma secundaria por linfomas en estadio avanzado y con alta carga tumoral. Sin embargo, tan solo en contadas ocasiones la afectación esquelética se debe a un linfoma óseo primario y constituye, por tanto, la forma de presentación de esta enfermedad. Se describe el caso de un paciente con un linfoma óseo primario de estirpe B que debutó con lesiones vertebrales y compresión medular secundaria (AU)

Skeletal involvement in patients with non-Hodgkin's lymphoma (NHL) is not uncommon. It tends to be a late manifestation and usually occurs secondary to lymphomas in advanced stage, with high tumor burden. However, only in a few cases has skeletal involvement been attributed to a primary bone lymphoma and constitutes, therefore, the form of presentation of this disease. We describe the case of a patient with primary B-cell lymphoma of the bone that appeared with vertebral lesions and secondary spinal compression (AU)

Chimerism detection by short tandem repeat analysis when donor and recipient genotypes are not known.

BACKGROUNDS: The analysis of chimerism after bone marrow transplantation by STR-PCR is frequently carried out with commercial kits designed for forensic purposes and including too many non informative STR. Furthermore, in routine clinical practice it is not uncommon to lack the pre-transplant genotype of the recipient or the donor, thus making it difficult to identify both components in the post-transplant genotype. The objective of this paper is to overcome these drawbacks by analyzing the informativity of STR markers from a perspective which can be applied whether the pretransplant genotypes are available or not, and selecting a minimum STR panel that allows an effective direct detection of chimerism. METHODS: DNA extraction, STR-PCR and fragment analysis of 15 STR in 90 donor-recipient pairs, 60 of which were part of the discovery set and 30 in a validation set. Loci were considered as informative when there were 3 or 4 different alleles in the combined genotypes of the recipient and the donor. RESULTS: The informativity varied between 41.6 and 76.6. The 4 most informative loci were D2S1338, D21S11, D18S51 and FGA. We could select a minimum set of 8 markers (D2S1338, D21S11, D18S51, FGA, VWA, D19S433, TH01 and D3S1358) that provided at least 3 informative loci in 95% of cases. CONCLUSION: This minimum STR panel may be an efficient way to detect and quantitate donor-recipient chimerism after transplantation.