ABSTRACT
Hyperimmune rabbit enterovirus-neutralizing diagnostic sera have a pronounced cytotoxic effect on the test tissue culture RD, HEp-2, and Vero cells and primary renal cells from African green monkeys (Cercopithecus aethiops). The specific features of this developing toxic effect, such as reduced proliferative activity and partial cytolysis, were revealed. The time course of changes in the generation of serum cytotoxicity during an immune response to repeated antigenic stimulation of donor animals is described. There is experimental evidence for no association of serum toxicity with specific neutralizing antibodies and with antibodies to cell culture antigens. Affinity chromatography is one of the possible techniques for purification of cytotoxic sera.
