Inhibición de rosetas E en la enfermedad hepática alcohólica / E rosette inhibition in alcoholic liver disease

Hemos estudiado la formación de Rosetas E y su inhibición en pacientes alcoholistas crónicos, con y sin cirrosis hepática. Los individuos fueron separados en 3 grupos: normales (n=21); alcoholistas sin lesión hepática (n=15) y alcoholistas con cirrosis (n=26). Se realizaron Test de Inhibición de Rosetas E, utilizando suero de los individuos en estudio, enfrentados con linfocitos de individuos sanos, efectuando 4 ensayos simultáneos: I.I. -S/A; I.I. -C/A; I.E. -S/A y I.E. -C/A. Analizados estadísticamente los resultados, observamos diferencias altamente significativas entre los pacientes con cirrosis y los controles, así como entre los cirróticos y los alcoholistas sin lesión hepática. Los ensayos que utilizaron la I.E. fueron los más eficientes para discriminar los grupos de individuos en estudio, siendo los que utilizan el suero no absorbido, los de mejor diferenciación. Con esta técnica observamos que 20/21 de los individuos control presentan inhibición inferior al 15//, y que todos los individuos con inhibición superior al 30// fueron cirróticos con sólo 2 excepciones. Proponemos la existencia de factores solubles en suero que provocan inhibición de la formación de rosetas E

Inhibición de rosetas E en la enfermedad hepática alcohólica / E rosette inhibition in alcoholic liver disease

Hemos estudiado la formación de Rosetas E y su inhibición en pacientes alcoholistas crónicos, con y sin cirrosis hepática. Los individuos fueron separados en 3 grupos: normales (n=21); alcoholistas sin lesión hepática (n=15) y alcoholistas con cirrosis (n=26). Se realizaron Test de Inhibición de Rosetas E, utilizando suero de los individuos en estudio, enfrentados con linfocitos de individuos sanos, efectuando 4 ensayos simultáneos: I.I. -S/A; I.I. -C/A; I.E. -S/A y I.E. -C/A. Analizados estadísticamente los resultados, observamos diferencias altamente significativas entre los pacientes con cirrosis y los controles, así como entre los cirróticos y los alcoholistas sin lesión hepática. Los ensayos que utilizaron la I.E. fueron los más eficientes para discriminar los grupos de individuos en estudio, siendo los que utilizan el suero no absorbido, los de mejor diferenciación. Con esta técnica observamos que 20/21 de los individuos control presentan inhibición inferior al 15//, y que todos los individuos con inhibición superior al 30// fueron cirróticos con sólo 2 excepciones. Proponemos la existencia de factores solubles en suero que provocan inhibición de la formación de rosetas E (AU)

[E rosette inhibition in alcoholic liver disease]. / Inhibición de rosetas E en la enfermedad hepática alcohólica.

Various studies performed in chronic alcoholic patients have demonstrated immunologic alterations, which have been described more often in patients with alcoholic hepatitis or liver cirrhosis. We have observed that the serum of some patients with alcoholic liver cirrhosis produces the inhibition of E rosette formation by T lymphocytes. This observation induced us to study E rosette formation and its probable inhibition by the serum of chronic alcoholic patients. Subjects were split into three groups: Group 1: n = 21. Normal individuals. Group 2: n = 15. Chronic alcoholic patients without cirrhosis. Group 3: n = 26. Chronic alcoholic patients with histologically demonstrated liver cirrhosis. E rosette and E rosette inhibition (TIRE) sera tests were performed utilizing subject's sera tested against lymphocytes of normal individuals not related to group 1. The results found are listed in detail in Table 1 (mean = -SD) and Figure 1 (median), for each of the test groups. We applied unifactorial variance analysis and observed highly significant differences between the groups studied in all tests performed. It was found that tests that utilized I.E. discriminate most efficiently among the three groups of patients and that those which utilize unabsorbed assay serum (S/A) yield the best differentiation. Using this last assay it was observed that 20/21 control individuals showed less than 15% inhibition. On this basis, we decided to separate the results into 15% regular intervals (Table 3). Inhibition above 30% was found only in cirrhotic patients with the exception of one individual of the control group and one non-cirrhotic alcoholic patient with no alcoholic liver disease.(ABSTRACT TRUNCATED AT 250 WORDS)

Inhibición de rosetas E en la enfermedad hepática alcohólica. / [E rosette inhibition in alcoholic liver disease]

Various studies performed in chronic alcoholic patients have demonstrated immunologic alterations, which have been described more often in patients with alcoholic hepatitis or liver cirrhosis. We have observed that the serum of some patients with alcoholic liver cirrhosis produces the inhibition of E rosette formation by T lymphocytes. This observation induced us to study E rosette formation and its probable inhibition by the serum of chronic alcoholic patients. Subjects were split into three groups: Group 1: n = 21. Normal individuals. Group 2: n = 15. Chronic alcoholic patients without cirrhosis. Group 3: n = 26. Chronic alcoholic patients with histologically demonstrated liver cirrhosis. E rosette and E rosette inhibition (TIRE) sera tests were performed utilizing subjects sera tested against lymphocytes of normal individuals not related to group 1. The results found are listed in detail in Table 1 (mean = -SD) and Figure 1 (median), for each of the test groups. We applied unifactorial variance analysis and observed highly significant differences between the groups studied in all tests performed. It was found that tests that utilized I.E. discriminate most efficiently among the three groups of patients and that those which utilize unabsorbed assay serum (S/A) yield the best differentiation. Using this last assay it was observed that 20/21 control individuals showed less than 15

inhibition. On this basis, we decided to separate the results into 15

regular intervals (Table 3). Inhibition above 30

was found only in cirrhotic patients with the exception of one individual of the control group and one non-cirrhotic alcoholic patient with no alcoholic liver disease.(ABSTRACT TRUNCATED AT 250 WORDS)