Investigation of ZIP4, ZO-1, and CLAUDIN-1 expression in thyroid tumours by immunohistochemistry and real-time polymerase chain reaction methods.

Thyroid neoplasms are the most common endocrine malignancies. ZIP4 is an intramembranous zinc trans membrane protein. Zinc plays a central role in the activation of transcription factors, and zinc transporters. This affects tumour migration, invasion, and cell proliferation. ZO-1 and Claudin-1 are important tight junction proteins whose amounts increase and decrease in various cancers. In this study, we aimed to investigate the expression of ZIP4, ZO-1, and Claudin-1 in thyroid tumours and the relationship of this expression with tumour types and prognostic parameters. ZIP4, ZO-1, and Claudin-1 were studied in all cases by immunohistochemical and Real-Time PCR methods. ZIP4 and Claudin-1 tended to be expressed more in cases with tumours, while ZO-1 in cases with and without tumours. Expression of ZIP4 and Claudin-1 by real-time polymerase chain reaction showed a significant difference between histological subtypes, and this difference was not observed with ZO-1. It was observed that the presence of metastasis increased with the expression of ZIP4 and Claudin-1, and there was no significant change with ZO-1. We think that Claudin-1 and ZIP4 expression can be used as an important marker in terms of showing poor prognosis and susceptibility to metastasis in thyroid tumours, and in developing targeted therapy.

A Rare Orbital Pathology: A Large Orbital Dermatofibrosarcoma Protuberans.

Dermatofibrosarcoma protuberans (DFSP) is a rare sarcoma of the dermis. It is a malignant, locally aggressive, and infiltrative tumor with frequent recurrence. In this case, a 44-year-old woman presented with a 15-year history of a swelling in the medial canthus of the right eye that caused tearing. Imaging revealed a septated mass isodense to soft tissue that had eroded the medial wall of the orbit. Macroscopic examination showed an elastic, gray-brown, encapsulated, irregular mass measuring 45x35x22 mm. The surgical margins were positive, so adjuvant radiotherapy was started. The patient was followed for 2 years without recurrence. According to the literature, this mass is the largest orbital DFSP treated by globe-sparing primary resection.