Production and properties of a laccase isozyme (FvLcc3) from the edible mushroom Flammulina velutipes, which is undetectable under the culture condition for fruiting body formation.

Extracellular laccase isozyme (FvLcc3) from the edible mushroom Flammulina velutipes was found to be undetectable under the culture condition for fruiting body formation. FvLcc3 was purified and determined to be an approximately 53-kDa monomeric protein. FvLcc3 showed the highest catalytic efficiency (kcat/Km) toward 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) followed by 2,6-dimethoxyphenol and guaiacol and did not oxidize 3,4-dihydroxy-l-phenylalanine and l-tyrosine.