Immunisation against ringworm in cattle.

Immunisation against dermatomycoses in cattle was introduced in Gausdal, Norway, in 1979. A programme was implemented in which a six-year period of mandatory vaccination of all cattle was followed by vaccination on a voluntary basis. The prevalence of infected herds fell from 70 per cent in 1979 to 0 per cent in 1987. During the last five years, no newly infected herds have been registered in Gausdal. Proper disinfection of premises, the isolation of infected animals and other hygienic measures contributed to the successful result.

Optimization and scale up of the adsorption fractionation of cod pyloric caeca deoxyribonuclease using axial and radial flow columns.

The key step in the purification of a deoxyribonuclease (DNase) from extracts of cod (Gadus morhua L.) pyloric caeca, is the selective retention of the enzyme by anion exchange chromatography. The cod DNase purification on Q-Sepharose Fast Flow (Pharmacia) was optimized, using a 60 ml fixed-bed column. In combination with titration curve analysis, we have screened the effect of buffer pHs, feed conductivity and protein loading, on the product recovery and purity. We have developed elution conditions which allow effective separation of the cod DNase from bounded impurities, such as proteinases and nucleic acids. Low levels of these impurities were regarded as essential for the desired product quality. The optimum resolution and maximum purification (ca. 20-fold increase in specific activity) of DNase, was, however, achieved at low protein loading (2.6 mg ml-1 gel), corresponding to less than 4% of the dynamic bed capacity. Scale-up to a 2.5 l pilot scale column (axial flow) and a 0.25 l radial flow column showed that the separation and yield obtained at laboratory scale was retained, and was independent of column geometry and bed height. The implications for a production scale scenario of 100 g of fractionated protein, are also discussed, as well as process hygiene. The optimization described herein adds further knowledge to the treatment of fish waste and the downstream processing of valuable biochemicals from marine raw material.

[Condoms and adolescents. A prospective study of a new method of teaching]. / Kondomer og unge. Prospektiv undersøgelse af en ny undervisnigsmetode.

A prospective investigation was carried out with the object of assessing the effect of supplementary sex education for 1.3 hours in addition to the compulsory sex education in Danish elementary schools. One doctor did the teaching and practice with a condom was introduced as a new method of teaching. On an average, seven weeks before and seven weeks after this teaching session, these pupils completed a questionnaire. Altogether 451 pupils from the eighth and ninth classes participated. Great changes had occurred in the methods of contraception employed by the adolescents and in their attitudes to these from 1982 to date. Many more of the adolescents consider today that condoms provide the best form of contraception as compared with oral contraception. Nevertheless, only 9% of the adolescents were able to use a condom correctly. After the teaching session, knowledge about the correct use of a condom was significantly better and more had a positive attitude to condoms. A balance in information about methods of contraception is recommended and the young people should be sent to their own general practitioners for individual advice. On the basis of the results of this investigation, this teaching should be given already to pupils in the eighth classes.

PIP: A prospective investigation was conducted to assess the effect of supplementary sex education for 1.3 house in addition to the compulsory sex education component in Danish elementary schools. 1 doctor did the teaching and practice with a condom was introduced as a new element in the teaching process. Students completed questionnaires both 7 weeks prior to 7 weeks after this teaching session; a total of 451 pupils from the 8th and 9th grades participated. From 1982 to the current time, there were great changes in the methods of contraception used by adolescents as well as in their attitudes. While more adolescents today consider the condom to provide the best form of contraception, nevertheless only 9% were able to use one correctly. After the teaching session, knowledge about how to use a condom correctly was significantly better and there was an overall improvement in attitude towards this method. A balance in information about methods of contraceptive is recommended and young people should see their own physicians for individual advice. The results of this investigation indicate that this additional teaching component is essential for those pupils already in the 8th grade. (author's modified)

Vaccination of cattle against ringworm caused by Trichophyton verrucosum.

A live-trichophyton vaccine against ringworm in cattle which was based on an attenuated strain of Trichophyton verrucosum was tested in controlled challenge-exposure experiments and in field trials. On the basis of data involving approximately 400,000 vaccinated animals over a 5-year period, it was concluded that trichophytosis in cattle can be controlled by immunoprophylaxis. Side effects associated with the vaccination occur infrequently.