Rapid and sensitive electrochemical detection of oxidized form of glutathione in whole blood samples using Bi-metallic nanocomposites.

We report a facile one-pot synthesis of bimetallic nickel-gold (Ni-Au) nanocomposite for ultra-sensitive and selective electrochemical detection of oxidized glutathione (GSSG) by electrochemical deposition on fluorine doped tin oxide (FTO) substrate. The electrodeposition of Ni-Au nanocomposite on FTO was confirmed by various characterization techniques such as field emission scanning electron microscopy (FE-SEM), X-ray diffractometer (XRD) and Fourier transform infra-red (FTIR) spectroscopy. The cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) was utilized for the electrochemical characterization of glutathione reductase (GR)/Ni-Au/FTO working electrode at each stage of modification. The GR enzyme immobilized on the Ni-Au/FTO working electrode via glutaraldehyde cross-linking exhibited excellent selectivity against GSSG in the presence of nicotinamide adenine dinucleotide phosphate (NADPH). The immobilized GR enzyme breaks down the GSSG to reduced glutathione (GSH) and converting NADPH to NADP+ whereby generating an electron for the electrochemical sensing of GSSG. The synergistic behavior of bimetals and good electro-catalytic property of the fabricated sensor provided a broad linear detection range from 1 fM to 1 µM with a limit of detection (LOD) of 6.8 fM, limit of quantification (LOQ) of 20.41 fM and sensitivity of 0.024 mA/µM/cm2. The interference with other molecules such as dopamine, glycine, ascorbic acid, uric acid and glucose was found to be negligible due to the better selectivity of GR enzyme towards GSSG. The shelf-life and response time of the fabricated electrode was found to be 30 days and 32 s, respectively. The real sample analysis of GSSG in whole blood samples showed average recovery percentage from 95 to 101% which matched well with the standard calibration plot of the fabricated sensor with relative standard deviation (RSD) below 10%.

Ultra-Sensitive Impedimetric Immunosensor Using Copper Oxide Quantum Dots Grafted on the Gold Microelectrode for the Detection of Parathion.

The extensive use of organophosphates (OPs) pollutes the environment, leading to serious health hazards for human beings. The current need is to fabricate a sensing platform that will be sensitive and selective towards the detection of OPs at trace levels in the nM to fM range. With this discussed in the present report, an ultra-sensitive immunosensing platform is developed using digestive-ripened copper oxide quantum dots grafted on a gold microelectrode (Au-µE) for the impedimetric detection of parathion (PT). The copper oxide quantum dots utilized in this study were of ultra-small size with a radius of approximately 2 to 3 nm and were monodispersed with readily available functional groups for the potential immobilization of antibody parathion (Anti-PT). The miniaturization is achieved by the utilization of Au-µE and the microfluidic platform utilized has the sample holding capacity of about 2 to 10 µL. The developed immunosensor provided a wide linear range of detection from 1 µM to 1 fM. The lower Limit of Detection (LoD) for the developed sensing platform was calculated to be 0.69 fM, with the sensitivity calculated to be 0.14 kΩ/nM/mm2. The stability of the sensor was found to be ~40 days with good selectivity. The developed sensor has the potential to integrate with a portable device for field applications.

Design and development of a portable resistive sensor based on α-MnO2 /GQD nanocomposites for trace quantification of Pb(II) in water.

The occurrence of heavy metal ions in food chain is appearing to be a major problem for mankind. The traces of heavy metals, especially Pb(II) ions present in water bodies remains undetected, untreated, and it remains in the food cycle causing serious health hazards for human and livestock. The consumption of Pb(II) ions may lead to serious medical complications including multiple organ failure which can be fatal. The conventional methods of heavy metal detection are costly, time-consuming and require laboratory space. There is an immediate need to develop a cost-effective and portable sensing system which can easily be used by the common man without any technical knowhow. A portable resistive device with miniaturized electronics is developed with microfluidic well and α-MnO2 /GQD nanocomposites as a sensing material for the sensitive detection of Pb(II). α-MnO2 /GQD nanocomposites which can be easily integrated with the miniaturized electronics for real-time on-field applications. The proposed sensor exhibited a tremendous potential to be integrated with conventional water purification appliances (household and commercial) to give an indication of safety index for the drinking water. The developed portable sensor required low sample volume (200 µL) and was assessed within the Pb(II) concentration range of 0.001 nM to 1 uM. The Limit of Detection (LoD) and sensitivity was calculated to be 0.81 nM and 1.05 kΩ/nM/mm2 , and was validated with the commercial impedance analyser. The shelf-life of the portable sensor was found to be ∼45 days.

Microfluidic Affinity Sensor Based on a Molecularly Imprinted Polymer for Ultrasensitive Detection of Chlorpyrifos.

The persistent use of pesticides in the agriculture field remains a serious issue related to public health. In the present work, molecularly imprinted polymer thin films were developed using electropolymerization of pyrrole (py) onto gold microelectrodes followed by electrodeposition for the selective detection of chlorpyrifos (CPF). The molecularly imprinted polymer (MIP) was synthesized by the electrochemical deposition method, which allowed in-line transfer of MIP on gold microelectrodes without using any additional adhering agents. Various parameters such as pH, monomer ratio, scan rate, and deposition cycle were optimized for sensor fabrication. The sensor was characterized at every stage of fabrication using various spectroscopic, microscopic, and electrochemical techniques. The sensor requires only 2 µL of the analyte and its linear detection range was found to be 1 µM to 1 fM. The developed sensor's limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.93 and 2.82 fM, respectively, with a sensitivity of 3.98 (µA/(µM)/ mm2. The sensor's shelf life was tested for 70 days. The applicability of the sensor in detecting CPF in fruit and vegetable samples was also assessed out with recovery % between 91 and 97% (RSD < 5%). The developed sensor possesses a huge commercial potential for on-field monitoring of pesticides.

Electro-deposited nano-webbed structures based on polyaniline/multi walled carbon nanotubes for enzymatic detection of organophosphates.

We report the development of an ultrasensitive electrochemical sensor using polyaniline (PANi) and carboxyl functionalized multi-walled carbon nanotubes (fMWCNT) for the detection of organophosphates (OPs) in real samples. The sensor was tested in the linear concentration range of 10 ng/L to 120 ng/L. The limit of detection (LoD) was found to be 8.8 ng/L with sensitivity 0.41 mA/ng/L/cm2 for chlorpyrifos (CPF); and 10.2 ng/L with sensitivity 0.58 mA/ng/L/cm2 for methyl parathion (MP). The vegetable samples (cucumber) were also tested. The average % recovery for CPF and MP were found to be 98.05% and 96.63% respectively. The developed sensor showed stability for a period of 30 days. The interference of the sensor was studied with heavy metals (cadmium (Cd), chromium (Cr), lead (Pb), arsenic (As)) which was found to be < 10%. The developed sensor will play a major role in real-time monitoring of food products, leading to food safety.

Paper based point of care immunosensor for the impedimetric detection of cardiac troponin I biomarker.

Advancements in health care monitoring demand a rapid, accurate and reliable early diagnosis of "Heart Attack" (acute myocardial infarction) with an objective to develop a cost-effective, rapid and label-free point of care diagnostic test kit for the detection of cardiac troponin I (cTnI) on paper-based multi-frequency impedimetric transducers. Paper based sensing platforms were developed by integrating carboxyl group functionalized multi-walled carbon nanotubes (MWCNT) with antibodies of cardiac troponin I (anti-cTnI) biomarker and was characterized using Electrochemical Impedance Spectroscopy (EIS). Various concentrations of cTnI with anti cTnI were studied as a function of impedance change. The suitability of the proposed immunosensor is demonstrated by spiking cTnI in blood serum samples. The limit of detection (LoD) and sensitivity of the proposed sensor was determined to be 0.05 ng/mL and 1.85 mΩ/ng/mL respectively, with a response time of ~1 min. The shelf life of the fabricated sensor was nearly 30 days. The rapid response, very low detection limit, and cost effectiveness offer a portable platform to detect cTnI in blood serum samples. The proposed immunosensor, therefore, offers an affordable healthcare diagnostic platform in resource limited areas.

Electrochemical micro analytical device interfaced with portable potentiostat for rapid detection of chlorpyrifos using acetylcholinesterase conjugated metal organic framework using Internet of things.

An Electrochemical micro Analytical Device (EµAD) was fabricated for sensitive detection of organophosphate pesticide chlorpyrifos in the food chain. Gold microelectrode (µE) modified with Zinc based Metal Organic Framework (MOF-Basolite Z1200) and Acetylcholinesterase (AChE) enzyme served as an excellent electro-analytical transducer for the detection of chlorpyrifos. Electrochemical techniques such as Cyclic Voltammetry (CV), Electrochemical Impedance Spectroscopy (EIS) and Differential Pulse Voltammetry (DPV) were performed for electrochemical analysis of the developed EµAD. The sensor needs only 2 µL of the analyte and it was tested within the linear range of 10 to 100 ng/L. The developed EµAD's limit of detection (LoD) and sensitivity is 6 ng/L and 0.598 µ A/ng L-1/mm2 respectively. The applicability of the device for the detection of chlorpyrifos from the real vegetable sample was also tested within the range specified. The fabricated sensor showed good stability with a shelf-life of 20 days. The EµAD's response time is of 50 s, including an incubation time of 20 s. The developed EµAD was also integrated with commercially available low-cost, handheld potentiostat (k-Stat) using Bluetooth and the results were comparable with a standard electrochemical workstation.