Large-scale analysis of small molecule-RNA interactions using multiplexed RNA structure libraries.

The large-scale analysis of small-molecule binding to diverse RNA structures is key to understanding the required interaction properties and selectivity for developing RNA-binding molecules toward RNA-targeted therapies. Here, we report a new system for performing the large-scale analysis of small molecule-RNA interactions using a multiplexed pull-down assay with RNA structure libraries. The system profiled the RNA-binding landscapes of G-clamp and thiazole orange derivatives, which recognizes an unpaired guanine base and are good probes for fluorescent indicator displacement (FID) assays, respectively. We discuss the binding preferences of these molecules based on their large-scale affinity profiles. In addition, we selected combinations of fluorescent indicators and different ranks of RNA based on the information and screened for RNA-binding molecules using FID. RNAs with high- and intermediate-rank RNA provided reliable results. Our system provides fundamental information about small molecule-RNA interactions and facilitates the discovery of novel RNA-binding molecules.

Model-based analysis of multi-UAV path planning for surveying postdisaster building damage.

Emergency responders require accurate and comprehensive data to make informed decisions. Moreover, the data should be acquired and analyzed swiftly to ensure an efficient response. One of the tasks at hand post-disaster is damage assessment within the impacted areas. In particular, building damage should be assessed to account for possible casualties, and displaced populations, to estimate long-term shelter capacities, and to assess the damage to services that depend on essential infrastructure (e.g. hospitals, schools, etc.). Remote sensing techniques, including satellite imagery, can be used to gathering such information so that the overall damage can be assessed. However, specific points of interest among the damaged buildings need higher resolution images and detailed information to assess the damage situation. These areas can be further assessed through unmanned aerial vehicles and 3D model reconstruction. This paper presents a multi-UAV coverage path planning method for the 3D reconstruction of postdisaster damaged buildings. The methodology has been implemented in NetLogo3D, a multi-agent model environment, and tested in a virtual built environment in Unity3D. The proposed method generates camera location points surrounding targeted damaged buildings. These camera location points are filtered to avoid collision and then sorted using the K-means or the Fuzzy C-means methods. After clustering camera location points and allocating these to each UAV unit, a route optimization process is conducted as a multiple traveling salesman problem. Final corrections are made to paths to avoid obstacles and give a resulting path for each UAV that balances the flight distance and time. The paper presents the details of the model and methodologies, and an examination of the texture resolution obtained from the proposed method and the conventional overhead flight with the nadir-looking method used in 3D mappings. The algorithm outperforms the conventional method in terms of the quality of the generated 3D model.