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1.
Psychiatr Rehabil J ; 2024 Feb 15.
Article in English | MEDLINE | ID: mdl-38358691

ABSTRACT

OBJECTIVE: Promoting leisure participation requires a collaborative approach that emphasizes personal interests, strengths, and motivations. The purpose of this article was to test the effectiveness of the Independence through Community Access and Navigation (ICAN) intervention on community participation, recreation participation, and positive emotions among individuals with schizophrenia spectrum disorders. Using motivational interviewing and an individualized placements and support framework, the ICAN intervention focuses on working with participants to identify and participate in personally meaningful leisure activities by connecting with personal motivations and mainstream community opportunities. METHOD: This randomized controlled trial was conducted with 74 participants diagnosed with schizophrenia with assessments conducted at baseline and posttreatment. Intervention effects were examined with repeated-measures analysis of variance (ANOVA). Multiple regression analysis was also performed using a change score as an outcome variable and baseline negative symptoms score, condition, and interaction as predictors. RESULTS: There was no significant main effect of ICAN on positive emotions, recreation participation, or community participation; however, among those in the experimental group, those with impairments in motivation and pleasure experienced improvements in community participation. CONCLUSIONS AND IMPLICATIONS FOR PRACTICE: For individuals experiencing greater negative symptoms, a supported leisure intervention may be an effective strategy to explore personal motivations and increase leisure participation. Future research should test the intervention effectiveness specifically targeting a larger sample of individuals with more severe negative symptoms. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

2.
Community Ment Health J ; 60(2): 308-316, 2024 02.
Article in English | MEDLINE | ID: mdl-37498513

ABSTRACT

There is a high prevalence of loneliness among adults with serious mental illness (SMI) with most research focusing on stable contributing factors. This study sought to identify the role of dispositional loneliness and internalized stigma, as well as the momentary feelings of acceptance on experiential loneliness among adults with SMI. Data were collected using ecological momentary assessment via smart phones, and 89 adults with a SMI were included. Hierarchical linear modeling was used to identify the role of dispositional and experience factors in experiential loneliness. Findings indicated that (a) dispositional internalized stigma, (b) being at home, (c) being alone and, (d) a cross-level interaction between dispositional loneliness and feelings of acceptance best fit the data. The relationship of acceptance to experiential loneliness was strongest among the most lonely. Supporting people with SMI to develop social connections contributing to their relational value may enhance feelings of acceptance and reduce loneliness.


Subject(s)
Loneliness , Mental Disorders , Adult , Humans , Emotions , Social Stigma , Personality
3.
Psychiatr Rehabil J ; 46(4): 368-372, 2023 Dec.
Article in English | MEDLINE | ID: mdl-37956063

ABSTRACT

OBJECTIVE: Severe loneliness infrequently occurs in the general population but has very significant impacts on health and quality of life. This study examined the extent to which severe loneliness is experienced by adults with serious mental illnesses (SMIs) relative to adults in the general population and its possible implications for psychiatric rehabilitation services. METHOD: Data were gathered from samples of individuals with SMI (N = 231) and a general community sample of adults (N = 300) using the University of California, Los Angeles Loneliness Scale. RESULTS: The results indicate that loneliness was much greater among those with SMI than the general adult population sample (Cohen's d = 1.220) and approximately 41% of the participants with SMI were "severely lonely" versus 7.3% of the non-SMI adult sample. CONCLUSIONS AND IMPLICATIONS FOR PRACTICE: Severe loneliness is extremely common among individuals with SMI. Psychiatric rehabilitation services that focus on socialization and mattering are needed to address this significant public health issue. (PsycInfo Database Record (c) 2024 APA, all rights reserved).


Subject(s)
Mental Disorders , Psychiatric Rehabilitation , Adult , Humans , Loneliness/psychology , Mental Disorders/rehabilitation , Quality of Life , Los Angeles
4.
Int J Soc Psychiatry ; 69(3): 559-566, 2023 05.
Article in English | MEDLINE | ID: mdl-36036232

ABSTRACT

BACKGROUND: People with serious mental illnesses (SMI) have higher levels of loneliness than the general population. Furthermore, people with SMI tend to be less satisfied with their housing and tend to move more frequently. AIM: This study aims to examine relationships between housing variables (whom they live with, duration of residence, and satisfaction) and loneliness among individuals with SMI. METHODS: Data were collected from 188 adults with SMI in greater Philadelphia area. Classification and Regression Trees (CART) were used to examine whether whom they live with, duration of residence, and housing satisfaction were associated with loneliness. RESULTS: Housing satisfaction was found to be the most prominent predictor of loneliness. Those who were unsatisfied with their overall housing conditions always had the highest level of loneliness, regardless of other factors. Even if they were satisfied with their housing conditions, their loneliness was higher if they had just moved to the new residence. Participants had lower loneliness the longer they lived in a residence and had the lowest loneliness levels after about three years. CONCLUSION: Housing is associated with loneliness among people with SMI. Psychiatric service providers should increase support to factors contributing to housing satisfaction and duration of residence, including active engagement in the community.


Subject(s)
Housing , Mental Disorders , Adult , Humans , Mental Disorders/psychology , Loneliness/psychology
5.
Am J Orthopsychiatry ; 92(6): 702-710, 2022.
Article in English | MEDLINE | ID: mdl-36174138

ABSTRACT

Promoting community participation is a core goal of behavioral health services for persons with serious mental illness (SMI) regardless of their racial/ethnic background. While practitioners are trained to respect diversity and culture, little is known about how racial/ethnic minorities participate in their communities during the recovery process. This is especially true for Black adults, who are disproportionately represented in current services. This study sought to compare differences in community participation experiences and interests between Black and White adults with SMI. Secondary data (n = 799) were analyzed from studies involving Black and White adults with SMI using the Temple University Community Participation Measure. Regression analyses were conducted to identify potential differences in four areas: amount of participation in a variety of community activities; number of activities participants deemed important; number of important activities actually performed; and the percentage of important activities performed as much as desired (i.e., sufficiency). Compared to White adults, Black adults had greater amounts of community participation overall and in a number of specific community activities (e.g., worship, visiting park/recreation center). Black adults also reported more community activities as important and were more likely to engage in their important activities. Relative to White adults, results suggest that Black adults with SMI were interested in and engaged more in social activities-potentially enhancing their sense of group solidarity and personal mental health recovery. Accordingly, practitioners should be aware of, and attend to, possible racial/ethnic differences in community participation, and its possible role in promoting or hindering recovery across diverse populations. (PsycInfo Database Record (c) 2022 APA, all rights reserved).


Subject(s)
Black People , Mental Disorders , White , Adult , Humans , Community Participation , Mental Disorders/psychology
6.
J Psychosoc Rehabil Ment Health ; 9(3): 283-292, 2022.
Article in English | MEDLINE | ID: mdl-35223375

ABSTRACT

Community participation is associated with physical, cognitive, and mental health benefits for people with serious mental illnesses (SMI) and is recognized as a critical component of health functioning. Developing reliable measurement of participation in different cultural contexts and languages is important to expanding knowledge in this area. The aim of this study was to translate a psychometrically sound English-language community participation measure into Japanese and examine its test-reliability with a population of Japanese people with SMI. Self-reported data were gathered twice from 253 individuals within 48 h using the Temple University Community Participation-Japanese version (TUCP-J) at Type-B Continuous Employment Support Centers in Japan between November 2020 and February 2021. Participant responses were similar on four of the five participation subscales. At the item-level, participants provided consistent responses on 26 out of 27 of the items about amount of participation and had high item-level concordance (77-93%) on their ratings of the importance (Yes; No) of each participation activity and their reported participation sufficiency (Enough; Not Enough; Too Much: 73-88%). Overall, the results demonstrated strong evidence of test-retest reliability of the TUCP-J and identified a number of areas that were important to respondents, but where they were reporting not doing enough.

7.
Appl Opt ; 61(32): 9716-9736, 2022 Nov 10.
Article in English | MEDLINE | ID: mdl-36606914

ABSTRACT

The Sunrise chromospheric infrared spectropolarimeter (SCIP) installed in the international balloon experiment sunrise iii will perform spectropolarimetric observations in the near-infrared band to measure solar photospheric and chromospheric magnetic fields simultaneously. The main components of SCIP for polarization measurements are a rotating wave plate, polarization beam splitters, and CMOS imaging sensors. In each of the sensors, SCIP records the orthogonal linearly polarized components of light. The polarization is later demodulated on-board. Each sensor covers one of the two distinct wavelength regions centered at 770 and 850 nm. To retrieve the proper circular polarization, the new parameter R, defined as the 45° phase shifted component of Stokes V in the modulation curve, is introduced. SCIP is aimed at achieving high polarization precision (1σ<3×10-4 of continuum intensity) to capture weak polarization signals in the chromosphere. The objectives of the polarization calibration test presented in this paper are to determine a response matrix of SCIP and to measure its repeatability and temperature dependence to achieve the required polarization precision. Tolerances of the response matrix elements were set after considering typical photospheric and chromospheric polarization signal levels. We constructed a feed optical system such that a telecentric beam can enter SCIP with the same f-number as the light distribution instrument of the sunrise iii telescope. A wire-grid linear polarizer and achromatic wave plate were placed before SCIP to produce the known polarization. The obtained response matrix was close to the values expected from the design. The wavelength and spatial variations, repeatability, and temperature dependence of the response matrix were confirmed to be smaller than tolerances.

8.
Int J Soc Psychiatry ; 68(8): 1689-1697, 2022 12.
Article in English | MEDLINE | ID: mdl-34894828

ABSTRACT

BACKGROUND: People with serious mental illnesses have elevated levels of depressive symptoms. Limited engagement in meaningful activities, such as work, social interactions, volunteering, and participation in faith, are one plausible explanation for this. Increased community participation over time may be associated with decreased depressive symptoms. AIM: Examine whether an increase in participation over time predicts a decrease in depression after controlling for depression at the baseline. METHODS: Participants were 183 adults with schizophrenia spectrum, bipolar disorder, or major depressive disorder who completed the Hopkins Symptom Index - Depression subscale and the Temple University Community Participation Measure. Participants completed these measures at baseline and either a 12- or 24-month follow-up timepoint. Multiple regression analyses were conducted with the depression score as a dependent variable and changes in community participation as a predictor variable. Demographics, baseline depression score, and time interval between baseline and last observation were entered as control variables. RESULTS: Endorsing more activities as important, participating in more important areas that are important, and participating 'enough' in more important areas over time were each significant predictors of decreases in depression. CONCLUSION: These findings enhance the connection between community participation and depression and suggest that a focus on participation may be important in terms of boosting both community functioning and treatment goals.


Subject(s)
Bipolar Disorder , Depressive Disorder, Major , Schizophrenia , Adult , Humans , Depression/diagnosis , Depressive Disorder, Major/diagnosis , Community Participation , Bipolar Disorder/therapy , Schizophrenia/diagnosis
9.
Am J Orthopsychiatry ; 91(5): 598-606, 2021.
Article in English | MEDLINE | ID: mdl-34060888

ABSTRACT

Community participation is important to the well-being of people with serious mental illnesses. While theories suggest that depressive symptoms can negatively impact community participation, evidence from previous studies was inconclusive. This study analyzed the relationship between severity of depressive symptoms and various participation constructs including number of days of participation in various community activities, breadth of interests in participating in community activities, and satisfaction with the amount to which one participates. A national sample of individuals with serious mental illnesses (n = 296) was employed. Overall, those who were severely depressed had fewer total days of participation, reported fewer participation areas as important, were less likely to participate in those areas that were important to them, and were less likely to participate as much as they wanted to in areas that were important to them. This relationship generally remained even after controlling for demographics. Overall, the findings from this study suggest that greater attention should be paid to the relationship between depressive symptoms and community participation in this population, including explorations that examine how the promotion of participation may have an impact on depressive symptoms. (PsycInfo Database Record (c) 2021 APA, all rights reserved).


Subject(s)
Depression , Mental Disorders , Community Participation , Depression/epidemiology , Humans , Mental Disorders/epidemiology
10.
Am J Orthopsychiatry ; 91(1): 1-8, 2021.
Article in English | MEDLINE | ID: mdl-32853006

ABSTRACT

Individuals with serious mental illnesses generally spend extensive amounts of time at home and alone. The aim of the current study was to examine differences in emotional states between being at home and being in the community, and between being alone and being with others. Ecological momentary assessment was utilized 3 times per day over 14 days with 91 individuals with serious mental illnesses to assess where they were, who they were with, and momentary feeling of depressed mood, loneliness, and happiness. A total of 2,257 data points were analyzed with hierarchical linear modeling. Participants were at home 70.6% of the time and alone 58.6% of the time. After controlling for diagnosis and symptoms, being in the community was associated with lower depressed mood, lower loneliness, and greater happiness, and being with others was associated with lower loneliness and greater happiness. There was no significant interaction effect between being at home and being alone on any emotional states. Being in the community and being with others contributed to more favorable emotional states among individuals with serious mental illnesses. These findings support the need to promote opportunities for community participation and interactions with others outside of one's home. Policies, programs, and clinical practices should align with the goal of supporting people with serious mental illnesses to actively engage in community living to facilitate their emotional well-being. (PsycInfo Database Record (c) 2021 APA, all rights reserved).


Subject(s)
Emotions , Mental Disorders , Happiness , Humans , Loneliness , Mental Health
11.
Psychiatr Serv ; 71(11): 1191-1194, 2020 11 01.
Article in English | MEDLINE | ID: mdl-32966173

ABSTRACT

OBJECTIVE: This study aimed to clarify the extent of community participation by individuals with serious mental illnesses in comparison with the general population. METHODS: Participants with serious mental illnesses (N=300) were recruited from 21 outpatient mental health organizations throughout the United States, and the participants without serious mental illnesses (N=300) were recruited from a stratified sample from across the United States. All participants completed the Temple University Community Participation Measure. RESULTS: The groups differed in community participation amount, number of important areas, breadth, and sufficiency of community participation. Differences between groups in amount of participation were not evident after analyses controlled for income and car ownership. However, differences in number of important areas, breadth of participation, and sufficiency remained after analyses controlled for demographic and economic factors. CONCLUSIONS: Car ownership and income are important factors in amount of community participation, but differences in other areas remain, plausibly affecting the health and wellness of persons with serious mental illnesses.


Subject(s)
Mental Disorders , Adult , Community Participation , Humans , Mental Disorders/epidemiology , United States/epidemiology
12.
J Community Psychol ; 48(2): 283-301, 2020 03.
Article in English | MEDLINE | ID: mdl-31596968

ABSTRACT

AIMS: Improved sense of coherence (SOC) can protect health among individuals with depression. Literature suggests that leisure behavior can improve SOC, yet empirical studies are lacking. The current study aimed to examine whether leisure behavior was associated with SOC and depression. METHODS: A cross-sectional online survey method was used with a sample of community-living adults (62% female, age 18-75 years) with a self-reported current or historical diagnosis of depression recruited from four different depression-related populations. RESULTS: The results of structural equation modeling found that leisure behavior predicted greater SOC as well as lower depressive symptoms. Greater SOC was associated with lower perceived stress and lower depressive symptoms. The total effect size, including both direct and indirect association between leisure behavior and depressive symptoms was 0.452. CONCLUSION: The findings suggested the potential benefits of leisure behavior on SOC and depressive symptoms.


Subject(s)
Depression/prevention & control , Depression/psychology , Leisure Activities/psychology , Sense of Coherence , Adult , Cross-Sectional Studies , Female , Health Behavior , Humans , Male , Middle Aged , Regression Analysis , Self Report , Young Adult
13.
Psychol Health Med ; 24(6): 654-666, 2019 07.
Article in English | MEDLINE | ID: mdl-30540200

ABSTRACT

Clinically significant depression has been consistently identified as a phenomenon directly linked to stress and stress coping. Leisure behavior as purposeful coping strategy (i.e. leisure coping) has been advanced as a potential approach to address depression; however, the leisure coping effect on depressive symptoms has not examined with two potential confounders: physical activity and extraversion. Physical activity's anti-depressant effect is widely known, and there is also a link between extraversion and lower depressive symptoms. The current study examined whether leisure coping has a significant effect on depressive symptoms among individuals with depression when physical activity and extraversion were taken into account. A cross-sectional online survey was conducted with respondents recruited from depression support group meetings, an online depression research portal, a state-wide research registry, and an outpatient psychiatric service provider. The analyses included a total of 152 adults (61.8% female) with self-reported depression who were age 18-75. A hierarchical regression analysis was performed to identify the strengths of association between leisure coping and depressive symptoms after accounting for covariates (demographics and perceived stress) and potential confounders (physical activity and extraversion). After a statistical control for physical activity and extraversion, leisure coping was significantly associated with lower depressive symptoms (ß = -.300, p < .01). Physical activity was a significant predictor of depressive symptoms in the model with leisure coping, but it became non-significant in the model with extraversion. The results suggested that leisure coping contributes to lower depressive symptoms independent from physical activity and extraversion. Introverts may be disadvantaged to take advantage of physical activity, but leisure coping might be one strategy to influence depressive symptoms. Further investigations should seek to verify this relationship as well as explore leisure coping as an intervention for depression.


Subject(s)
Adaptation, Psychological , Depression/psychology , Exercise/psychology , Extraversion, Psychological , Leisure Activities/psychology , Adolescent , Adult , Aged , Cross-Sectional Studies , Female , Humans , Male , Middle Aged , Young Adult
14.
Disabil Health J ; 11(2): 262-266, 2018 04.
Article in English | MEDLINE | ID: mdl-29107585

ABSTRACT

BACKGROUND: Adolescents with mobility impairments have fewer opportunities to challenge identity as an athlete through sport participation. OBJECTIVE/HYPOTHESIS: This study examined athletic identity impact of adolescent athletes with mobility impairments who participate in Paralympic Sport Clubs. METHODS: This cross-sectional web-survey design examined demographics (gender, academic level, hours of time spent in sports participation, and preferred amount of time in sport participation) and athletic identity through the Athletic Identity Measurement Scale (AIMS). Forty-seven adolescents (male = 34, female = 13) between the ages of 13-18 with mobility impairments who were currently participating in a Paralympic Sport Club were recruited for the study. RESULTS: Two factorial ANOVA examined the differences of AIMS scores by gender and academic level, and gender and hours of time spent in sports participation per week. Hours of time spent in sports participation by adolescent athletes with physical disabilities showed significant difference on athletes' identity. AIMS score increased along with augmented time spent in sport participation. However, gender and academic level made no significant difference on athletic identity. A one-way ANOVA test was applied to compare the effect of preferred amount of time in sport participation per week on scores for AIMS and showed significant group difference on AIMS. Participants who indicated more preferred amount of time in sport participation also scored higher on AIMS. CONCLUSIONS: These findings support the notion that hours of time spent and preferred amount of time in sports participation influences athletic identity. Sport participation, when available can influence athletic identity with this population.


Subject(s)
Athletes , Disabled Persons/psychology , Mobility Limitation , Self Concept , Social Identification , Sports , Adolescent , Analysis of Variance , Competitive Behavior , Cross-Sectional Studies , Female , Humans , Male
15.
J Spinal Cord Med ; 39(6): 693-698, 2016 11.
Article in English | MEDLINE | ID: mdl-26833021

ABSTRACT

OBJECTIVE: This exploratory study describes the problematic secondary health conditions among adults with a spinal cord injury (SCI) and the impact these health concerns have on social participation and daily life. DESIGN: Cross-sectional survey design. SETTING: A community-based rehabilitation program within the United States. PARTICIPANTS: Fifty-six adults (33 males and 23 females; age 18 to 73 [M = 39.4, SD = 12.7]) with SCI participating in the community-based rehabilitation program. METHODS: Subjects identified the top five problematic secondary health conditions related to his/her SCI, belief about the impact these conditions have on social participation and daily life, and if they believed the secondary health condition(s) were avoidable. RESULTS: The top problematic areas identified were bladder control, pain, bowel control, and pressure ulcers, and 73% felt these problems were unavoidable. In addition, more than 66% had each of these problems continuously during the last 12 months. When examining the impact of the problematic secondary health conditions, 75% identified that the primary problem had a significant impact on social participation and 64% identified it significantly impacted daily life. CONCLUSION: Although the majority of the participants were actively participating in a community-based rehabilitation wellness program, it appears that they thought engagement in social participation and daily life were negatively impacted by the secondary health conditions and unavoidable. The results suggested unfulfilled goals despite the emphasized efforts of medical providers to help manage the secondary conditions. Future research should examine why individuals with SCI still have a difficult time managing secondary health conditions.


Subject(s)
Pain/psychology , Pressure Ulcer/psychology , Social Participation , Spinal Cord Injuries/rehabilitation , Urinary Bladder, Neurogenic/psychology , Adolescent , Adult , Aged , Female , Humans , Male , Middle Aged , Pain/epidemiology , Pain/etiology , Pressure Ulcer/epidemiology , Pressure Ulcer/etiology , Spinal Cord Injuries/complications , Urinary Bladder, Neurogenic/epidemiology , Urinary Bladder, Neurogenic/etiology
16.
J Agric Food Chem ; 59(14): 7896-901, 2011 Jul 27.
Article in English | MEDLINE | ID: mdl-21696216

ABSTRACT

Alginase was purified from Gracilibacillus A7 and evaluated for its ability to produce elicitor-active oligosaccharides. The optimum conditions for the alginase reaction are as follows: temperature, 40 °C; pH, 8.0; alginate content, 0.3-0.7%; and the presence of Na(+) and Mg(2+) metal ions. The degree of polymerization (DP) decreased as the reaction time of the alginase progressed, achieving values of 5.4 and 3.3 after 240 and 300 min, respectively. The relative root length (RRL) of the Brassica campestris L. increased with the addition of oligosaccharides with reduced DP values. The oligosaccharides with lower DP values are effective in reducing the effect of salt stress on the activity of the superoxide dismutase (SOD) and guaiacol peroxidase (POD), and oligosaccharides with moderate DP values can reduce the increase in lipid peroxidation activities (as malondialdehyde content) induced by salt stress. These results suggest that oligosaccharides may act as osmoprotective agents during the plant germination process.


Subject(s)
Bacillaceae/enzymology , Bacterial Proteins/chemistry , Brassica/microbiology , Brassica/physiology , Oligosaccharides/chemistry , Polysaccharide-Lyases/chemistry , Bacillaceae/chemistry , Bacillaceae/metabolism , Bacterial Proteins/metabolism , Brassica/growth & development , Kinetics , Oligosaccharides/metabolism , Polymerization , Polysaccharide-Lyases/metabolism , Salts/metabolism , Stress, Physiological
17.
Extremophiles ; 15(2): 303-10, 2011 Mar.
Article in English | MEDLINE | ID: mdl-21331633

ABSTRACT

Using ectoine-excreting strain Halomonas salina DSM 5928(T), we developed a new process for high-efficiency production of ectoine, which involved a combined process of batch fermentation by growing cells and production by resting cells. In the first stage, batch fermentation was carried out using growing cells under optimal fermentation conditions. The second stage was the production phase, in which ectoine was synthesized and excreted by phosphate-limited resting cells. Optimal conditions for synthesis and excretion of ectoine during batch fermentation in a 10 l fermentor were 0.5 mol l(-1) NaCl and an initial monosodium glutamate concentration of 80 g l(-1) respectively. The pH was adjusted to 7.0 and the temperature was maintained at 33°C. In phosphate-limited resting cells medium, monosodium glutamate and NaCl concentration was 200 g l(-1) and 0.5 mol l(-1), respectively, as well as pH was 7.0. The total concentration of ectoine produced was 14.86 g l(-1), the productivity and yield of ectoine was 7.75 g l(-1) day(-1) and 0.14 g g(-1), respectively, and the percentage of ectoine excreted was 79%. These levels of ectoine production and excretion are the highest reported to date.


Subject(s)
Amino Acids, Diamino/biosynthesis , Halomonas/metabolism , Amino Acids, Diamino/chemistry , Bioreactors , Chromatography, High Pressure Liquid , Chromatography, Ion Exchange , Culture Media , Ethanol/chemistry , Fermentation , Glutamic Acid/chemistry , Hydrogen-Ion Concentration , Industrial Microbiology , Sodium Chloride/chemistry , Temperature , Time Factors
18.
Bioresour Technol ; 102(3): 2925-30, 2011 Feb.
Article in English | MEDLINE | ID: mdl-21145233

ABSTRACT

Composting of the Undaria pinnatifida (wakame) seaweed was conducted after inoculation with 6×10(8) CFU g(-1)Halomonas sp. AW4 and the alginate-degrading bacterium Gracilibacillus sp. A7. Inoculation with strains A7 and AW4 resulted in 27.8% and 24.7% degradation of U. pinnatifida dry mass after 168 h, whereas only 17.5% degradation occurred in the uninoculated control. The C/N ratio decreased in the A7 and AW4 inoculated compost by 7.0% and 9.2% after 72 h, but increased by 11.5% in the control. Inoculation with A7 resulted in 2.8 times faster degradation of alginate and 1.2 and 1.6 times higher levels of reducing sugars and unsaturated sugars than inoculation with AW4. The compost produced from the inoculation with A7 had low plant toxicity as measured by germination experiment. The results suggest that inoculation of wakame with alginate-degrading bacteria not only shortened the length of composting but also created seaweed compost with good fertilizer qualities.


Subject(s)
Bacillus/physiology , Halomonas/physiology , Soil Microbiology , Undaria/physiology , Coculture Techniques/methods , Marine Biology
19.
Can J Microbiol ; 56(12): 1020-7, 2010 Dec.
Article in English | MEDLINE | ID: mdl-21164572

ABSTRACT

The moderately halophilic bacterium Salinivibrio costicola subsp. yaniae showed an extremely fast growth rate. Optimal growth was observed in artificial seawater containing 1.4 mol/L NaCl and in MM63 media containing 0.6 mol/L NaCl. We analyzed a variety of compatible solutes that had accumulated in this strain grown in the media. The supplementation effect of the compatible solutes glycine betaine, glutamate, and ectoine to the growth of S. costicola subsp. yaniae was examined. Glycine betaine and glutamate had no supplementation effect on the fast growth rate. Growth of salt-sensitive mutants MU1 and MU2, both of which were defective in the ability to synthesize ectoine, was not observed in MM63 medium in the presence of more than 1.0 mol/L NaCl. From these data, we conclude that ectoine was the predominant compatible solute synthesized in this bacterium that effected an extremely fast growth rate.


Subject(s)
Adaptation, Physiological , Amino Acids, Diamino/metabolism , Vibrionaceae/growth & development , Vibrionaceae/metabolism , Amino Acid Sequence , Amino Acids, Diamino/genetics , Betaine/metabolism , Betaine/pharmacology , Culture Media/chemistry , Culture Media/pharmacology , Glutamic Acid/metabolism , Glutamic Acid/pharmacology , Molecular Sequence Data , Mutation/genetics , Osmolar Concentration , Sequence Alignment , Sodium Chloride/pharmacology , Vibrionaceae/drug effects , Vibrionaceae/genetics
20.
Biocontrol Sci ; 15(2): 57-62, 2010 Jun.
Article in English | MEDLINE | ID: mdl-20616433

ABSTRACT

To investigate the adaptability to higher temperatures of Antarctic microorganisms persisting in low temperature conditions for a long time, Antarctic lake samples were incubated in several selection media at 25 degrees C and 30 degrees C. The microorganisms did not grow at 30 degrees C; however, some of them grew at 25 degrees C, indicating that the bacteria in Antarctic have the ability to grow at a wide range of temperatures. Total DNA was extracted from these microorganisms and amplified using the bacteria-universal primers. The amplified fragments were cloned, and randomly selected 48 clones were sequenced. The sequenced clones showed high similarity to the alpha-subdivision of the Proteobacteria with specific affinity to the genus Agrobacterium, Caulobacter and Brevundimonas, the ss-subdivision of Proteobacteria with specific affinity to the genus Cupriavidus, and Bacillus of the phylum Firmicutes. These results showed the presence of universal genera, suggesting that the bacteria in the Antarctic lake were not specific to this environment.


Subject(s)
Adaptation, Physiological , Bacteria/growth & development , Water Microbiology , Antarctic Regions , Bacteria/classification , Culture Media , Fresh Water/microbiology , Phylogeny , Temperature
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