ABSTRACT
This study evaluated the occurrence of granulomatous lymphadenitis and its association with Actinobacillus spp. in 151 653 slaughtered pigs. Markedly enlarged pulmonary hilar, mediastinal, mandibular or hepatic lymph nodes were detected in 6 castrated males. The cut surfaces showed multifocal yellow-white lesions. Histologically, gram-negative bacilli were visible in the centers of the lesions with asteroid bodies, epithelioid cells, and multinucleated giant cells. Dense fibrous connective tissue surrounded these granulomatous lesions. Immunohistochemically, the organisms reacted with polyclonal antibodies against Actinobacillus pleuropneumoniae serotype 2 in all 6 barrows. The organism was isolated from the lymph nodes of all 6 animals. The results indicate that the granulomatous lymphadenitis was associated with A. pleuropneumoniae serotype 2 and the disorder had a tendency to occur in slaughter barrows.
Subject(s)
Actinobacillus Infections/veterinary , Actinobacillus pleuropneumoniae , Lymphadenitis/veterinary , Swine Diseases/microbiology , Swine Diseases/pathology , Actinobacillus Infections/microbiology , Actinobacillus Infections/pathology , Actinobacillus pleuropneumoniae/classification , Actinobacillus pleuropneumoniae/immunology , Actinobacillus pleuropneumoniae/isolation & purification , Animals , Antibodies, Bacterial/immunology , Lymph Nodes/microbiology , Lymph Nodes/pathology , Lymphadenitis/microbiology , Lymphadenitis/pathology , Male , Serotyping/veterinary , SwineABSTRACT
A molecular biological survey on porcine norovirus (NoV) and sapovirus (SaV) was conducted in Toyama Prefecture, Japan, during fiscal year 2008. Both NoV and SaV were detected from swine fecal samples throughout the surveillance period, indicating that these viruses were circulating in this region. NoV strains detected in this study belonged to three genotypes that are known as typical swine NoVs. Although human NoVs were occasionally detected, it was unclear whether they replicated in pigs. As for SaV, genogroup VII (GVII) and other divergent genogroups were identified in addition to the dominant genogroup, GIII, which is the prototypic porcine SaV. In addition, 3 strains genetically related to human SaV were detected. Two of these 3 strains were closely related to human SaV GV. Our study showed that genetic diversification of porcine SaV is currently progressing in the swine population.
Subject(s)
Caliciviridae Infections/veterinary , Genetic Variation , Norovirus/isolation & purification , Sapovirus/isolation & purification , Swine Diseases/epidemiology , Swine Diseases/virology , Animals , Caliciviridae Infections/epidemiology , Caliciviridae Infections/virology , Cluster Analysis , Feces/virology , Genotype , Japan/epidemiology , Molecular Epidemiology , Molecular Sequence Data , Norovirus/classification , Norovirus/genetics , Phylogeny , RNA, Viral/genetics , Sapovirus/classification , Sapovirus/genetics , Sequence Analysis, DNA , SwineABSTRACT
A total of 14,818 slaughtered pigs were examined macroscopically. Of these, 25 pigs with porcine pleuropneumonia were collected and the relations among Actinobacillus spp. and granulomatous lesions in organs (lungs and tonsils) were evaluated. In the lungs, only Actinobacillus pleuropneumoniae serotype 2 was isolated from 20 of the pigs. Histologically, granulomatous pneumonia with A. pleuropneumoniae antigen was detected in 8 of the pigs. The antigen was visible in the centers of the lesions along with asteroid bodies, epithelioid cells and multinucleated giant cells. In the tonsils, granulomatous lesions were not detected, although A. pleuropneumoniae serotype 2 (5 pigs), serotype 7 (1 pig), Actinobacillus porcitonsillarum (1 pig) and Actinobacillus minor (1 pig) were isolated. The present survey suggests that multifocal granulomatous pneumonia in slaughter pigs could be highly associated with A. pleuropneumoniae serotype 2 infection.
