ABSTRACT
Osteomyelitis continues to be a severe problem worldwide, causing plenty of hospital admissions and entailing vast expenses. Previously, we developed a low-cost polymethyl-methacrylate (PMMA)-sorbitol based capsule system for local long-term drug delivery. In the present study we aimed to test the in vitro release of clindamycin capsules by high performance liquid chromatography. By the end of the clinically relevant period (42 days), the capsules released 70-100% of their load. Furthermore, the release kinetics suggested that an effective antimicrobial concentration may be maintained within the target area. Our findings indicate that these newly developed capsules may be a versatile device for local clindamycin delivery by providing efficient release and reducing financial burdens.
Subject(s)
Anti-Bacterial Agents/chemistry , Clindamycin/chemistry , Drug Delivery Systems , Osteomyelitis/drug therapy , Anti-Bacterial Agents/administration & dosage , Anti-Bacterial Agents/adverse effects , Anti-Bacterial Agents/economics , Capsules , Chromatography, High Pressure Liquid , Chronic Disease , Clindamycin/administration & dosage , Clindamycin/adverse effects , Clindamycin/economics , Delayed-Action Preparations/economics , Drug Compounding , Drug Delivery Systems/economics , Health Care Costs , Kinetics , Osteomyelitis/economics , Polymethyl Methacrylate/chemistry , Solubility , Sorbitol/chemistrySubject(s)
Adenosine Triphosphate/metabolism , Antiporters/metabolism , Ion Pumps/metabolism , Sodium-Potassium-Exchanging ATPase/metabolism , Amino Acid Sequence , Antiporters/chemistry , Calcium-Transporting ATPases/chemistry , Calcium-Transporting ATPases/metabolism , Humans , Ion Pumps/chemistry , Kinetics , Magnesium/metabolism , Mutagenesis, Site-Directed , Phosphates/metabolism , Phosphorylation , Protein Conformation , Recombinant Proteins/chemistry , Recombinant Proteins/metabolism , Sodium-Potassium-Exchanging ATPase/chemistryABSTRACT
We have proposed a model for part of the catalytic site of P-type pumps in which arginine in a signature sequence functions like lysine in P-loop-containing enzymes that catalyze adenosine 5'-triphosphate hydrolysis [Smirnova, I. N., Kasho, V. N., and Faller, L. D. (1998) FEBS Lett. 431, 309-314]. The model originated with evidence from site-directed mutagenesis that aspartic acid in the DPPR sequence of Na,K-ATPase binds Mg(2+) [Farley, R. A., et al. (1997) Biochemistry 36, 941-951]. It was developed by assuming that the catalytic domain of P-type pumps evolved from enzymes that catalyze phosphoryl group transfer. The functions of the positively charged amino group in P-loops are to bind substrate and to facilitate nucleophilic attack upon phosphorus by polarizing the gamma-phosphorus-oxygen bond. To test the prediction that the positively charged guanidinium group of R596 in human alpha(1) Na,K-ATPase participates in phosphoryl group transfer, the charge was progressively decreased by site-directed mutagenesis. Mutants R596K, -Q, -T, -M, -A, -G, and -E were expressed in yeast membranes, and their ability to catalyze phosphorylation with inorganic phosphate was evaluated by following (18)O exchange. R596K, in which the positive charge is retained, resembled the wild type. Substitution of a negative charge (R596E) resulted in complete loss of activity. The remaining mutants with uncharged side chains had both lowered affinity for inorganic phosphate and altered phosphate isotopomer distributions, consistent with increased phosphate-off rate constants compared to that of the wild type. Therefore, mutations of R596 strengthen our hypothesis that the oppositely charged side chains of the DPPR peptide in Na,K-ATPase form a quaternary complex with magnesium phosphate.
Subject(s)
Arginine/genetics , Mutagenesis, Site-Directed , Phosphates/metabolism , Sodium-Potassium-Exchanging ATPase/genetics , Sodium-Potassium-Exchanging ATPase/metabolism , Amino Acid Sequence/genetics , Animals , Arginine/metabolism , Binding Sites/genetics , Catalysis , Dogs , Enzyme Activation/genetics , Gene Expression Regulation, Enzymologic/genetics , Humans , Kinetics , Ouabain/metabolism , Oxygen Isotopes/metabolism , Phosphorylation , Protein Conformation , Saccharomyces cerevisiae/enzymology , Saccharomyces cerevisiae/genetics , Sodium-Potassium-Exchanging ATPase/biosynthesisABSTRACT
Cell surface ATPases (ecto-ATPases or E-ATPases) hydrolyze extracellular ATP and other nucleotides. Regulation of extracellular nucleotide concentration is one of their major proposed functions. Based on enzymatic characterization, the E-ATPases have been divided into two subfamilies, ecto-ATPases and ecto-ATP-diphosphohydrolases (ecto-ATPDases). In the presence of either Mg2+ or Ca2+, ecto-ATPDases, including proteins closely related to CD39, hydrolyze nucleoside diphosphates in addition to nucleoside triphosphates and are inhibited by millimolar concentrations of azide, whereas ecto-ATPases appear to lack these two properties. This report presents the first systematic kinetic study of a purified ecto-ATPDase, the chicken oviduct ecto-ATPDase (Strobel, R.S., Nagy, A.K., Knowles, A.F., Buegel, J. & Rosenberg, M.O. (1996) J. Biol. Chem. 271, 16323-16331), with respect to ATP and ADP, and azide inhibition. Km values for ATP obtained at pH 6.4 and 7.4 are 10-30 times lower than for ADP and the catalytic efficiency is greater with ATP as the substrate. The enzyme also exhibits complicated behavior toward azide. Variable inhibition by azide is observed depending on nucleotide substrate, divalent ion, and pH. Nearly complete inhibition by 5 mm azide is obtained when MgADP is the substrate and when assays are conducted at pH 6-6.4. Azide inhibition diminishes when ATP is the substrate, Ca2+ as the activating ion, and at higher pH. The greater efficacy of azide in inhibiting ADP hydrolysis compared to ATP hydrolysis may be related to the different modes of inhibition with the two nucleotide substrates. While azide decreases both Vmax and Km for ADP, it does not alter the Km for ATP. These results suggest that the apparent affinity of azide for the E.ADP complex is significantly greater than that for the free enzyme or E.ATP. The response of the enzyme to three other inhibitors, fluoride, vanadate, and pyrophosphate, is also dependent on substrate and pH. Taken together, these results are indicative of a discrimination between ADP and ATP by the enzyme. A mechanism of azide inhibition is proposed.
Subject(s)
Apyrase/antagonists & inhibitors , Azides/pharmacology , Enzyme Inhibitors/pharmacology , Adenosine Diphosphate/metabolism , Adenosine Triphosphate/metabolism , Animals , Chickens , Diphosphates/pharmacology , Fluorides/pharmacology , Hydrogen-Ion Concentration , Hydrolysis , Oviducts/drug effects , Oviducts/enzymology , Vanadates/pharmacologyABSTRACT
The chicken oviduct ecto-ATP diphosphohydrolase (ATPDase), a member of the ecto-ATPase family, was purified to homogeneity previously (Strobel, R. S., Nagy, A. K., Knowles, A. F., Buegel, J., and Rosenberg, M. O. (1996) J. Biol. Chem. 271, 16323-16331). It is an 80-kDa glycoprotein with high specific activity (approximately 1,000 micromol/min/mg with MgATP as the substrate) and hydrolyzes both nucleoside triphosphates and diphosphates. Using amino acid sequence information obtained from the purified enzyme, two partial cDNA clones were obtained using reverse transcriptase-polymerase chain reaction and library screening. This is the second ecto-ATPase family member and the first ecto-ATPDase to be cloned from information derived from purified proteins. The deduced primary sequence of the chicken oviduct ecto-ATPDase indicates a protein of 493 amino acid residues with a molecular mass of 54 kDa. The predicted orientation shows it to be anchored to the membrane by two transmembranous segments near the NH2 and COOH termini with very short intracytoplasmic peptides at either end. The bulk of the protein is extracellular and contains 12 potential N-glycosylation sites, several potential phosphorylation sites, and five sequences that are conserved in seven other related membrane proteins. Four of the conserved sequences, designated as apyrase conserved regions, are present in both ecto-ATPases and soluble E-type ATPases. The fifth conserved region, which occurs near the COOH terminus of the eight proteins, is observed only in the membrane-bound ecto-ATPases. Unexpectedly, sequence comparison revealed that the chicken oviduct ecto-ATPDase is equally distant from the two ecto-ATPases, which exhibit low activity toward ADP, and the four putative ecto-ATPDases, which are closely related to CD39.
Subject(s)
Apyrase/genetics , Oviducts/enzymology , Amino Acid Sequence , Animals , Apyrase/metabolism , Base Sequence , Chickens , Cloning, Molecular , Humans , Membrane Proteins/analysis , Molecular Sequence DataABSTRACT
Changes in residual ATP concentrations were investigated following subcellular fractionation of rat brain cortex after a prolonged period of status epilepticus induced by sequential administration of lithium and pilocarpine. After 2 h of continuous high-amplitude rapid spiking on EEG, we found significantly decreased levels of residual ATP in the homogenate and mitochondria fractions from status epilepticus rat brains compared to matched controls. No difference in residual ATP level was observed in the synaptosomal preparations of status epilepticus animals compared to controls. Inorganic phosphate concentration in the status animals was higher than controls in the cytosolic fraction only. F1-ATPase activity, an enzymatic indicator of mitochondrial ATP synthesis rate, was significantly higher in the status brains, whereas other mitochondrial enzymes were not different in the status and control rat groups. These findings, together with our earlier report of reduced synaptosomal ecto-ATPase activity, suggest that either the corresponding in vivo ATP concentrations were reduced as a result of status epilepticus or other biochemical changes had occurred that facilitated the hydrolysis of ATP following decapitation. Controls for and measurement of such other changes failed to provide an explanation for the observed changes in residual ATP.
Subject(s)
Adenosine Triphosphate/metabolism , Cerebral Cortex/metabolism , Status Epilepticus/metabolism , Animals , Cell Fractionation , Cell Membrane/drug effects , Cell Membrane/enzymology , Cell Membrane/metabolism , Cerebral Cortex/cytology , Cerebral Cortex/drug effects , Cytosol/drug effects , Cytosol/metabolism , Hydrolysis , Lithium , Male , Matched-Pair Analysis , Mitochondria/drug effects , Mitochondria/enzymology , Mitochondria/metabolism , Phosphates/metabolism , Pilocarpine , Proton-Translocating ATPases/metabolism , Rats , Rats, Sprague-Dawley , Status Epilepticus/chemically induced , Synaptosomes/drug effects , Synaptosomes/metabolism , Time FactorsABSTRACT
Considerable evidence indicates that ATP, acting intracellularly of as a neurotransmitter, can influence nerve cell physiology in a variety of ways. Defects in the functioning of ATP-metabolizing enzymes could therefore lead to disturbances in neurotransmission and creation of sustained neuronal discharges characteristic of status epilepticus. In this study we investigated synaptosomal ATPase changes in rat brains during lithium/pilocarpine-induced status epilepticus. After 2 h of continuous electroencephalographic spiking, both Mg(2+)- and Ca(2+)-dependent ecto-ATPases were significantly decreased in freshly prepared synaptosomal preparations from the status rats. The intracellularly acting Ca2+Mg(2+)-ATPase (Ca-pump) was also decreased, but no changes occurred in synaptosomal Na+K(+)-ATPase activity. The difference between ecto-ATPase activities of the control and status rat brains was not affected by repeated freezing-thawing and lengthy storage. Possible involvement of reduced synaptosomal divalent cation-dependent ATPases in the pathophysiology of status epilepticus is discussed.
Subject(s)
Adenosine Triphosphatases/metabolism , Cerebral Cortex/drug effects , Lithium/toxicity , Pilocarpine/toxicity , Status Epilepticus/enzymology , Analysis of Variance , Animals , Cerebral Cortex/enzymology , Chronic Disease , Drug Administration Schedule , Male , Rats , Rats, Sprague-Dawley , Status Epilepticus/chemically induced , Synaptosomes/drug effects , Synaptosomes/enzymologyABSTRACT
PURPOSE: Rhodopsin is a zinc-binding protein. We investigated the effect of low concentrations of zinc on the initial phosphorylation of rhodopsin. METHODS: Dark-adapted bovine rod outer segments (ROS) were incubated with (gamma 32P) ATP and 5 mM magnesium in the presence and absence of micromolar amounts of zinc. The ROS were exposed to light to initiate phosphorylation under conditions which allow only limited initial phosphorylation. RESULTS: We found that zinc enhanced the rhodopsin phosphorylation apparent on autoradiographies by several fold. Phosphorylation reactions conducted in the presence of potent phospho-opsin phosphatase inhibitors show a comparable zinc-enhanced phosphorylation of rhodopsin. Under our reaction conditions, ROS membranes also appear more red upon initial exposure to light when zinc is present. CONCLUSIONS: Zinc can increase initial rhodopsin phosphorylation, apparently acting at the substrate rhodopsin and not at relevant phosphatases or rhodopsin kinase. How zinc binding to rhodopsin might increase its ability to serve as a substrate for phosphorylation is under investigation.
Subject(s)
Eye Proteins , Rhodopsin/metabolism , Rod Cell Outer Segment/physiology , Zinc/pharmacology , Animals , Autoradiography , Binding Sites , Cattle , Dark Adaptation , Electrophoresis, Polyacrylamide Gel , G-Protein-Coupled Receptor Kinase 1 , Phosphorylation/drug effects , Phosphotransferases/metabolism , Protein Kinases/metabolism , Rhodopsin/drug effects , Rod Cell Outer Segment/cytology , Rod Cell Outer Segment/drug effects , SpectrophotometryABSTRACT
An ecto-ATP diphosphohydrolase (ATPDase) was purified to homogeneity from vesiculosomes shed from chicken oviduct. First, the ecto-ATPDase-enriched vesiculosomes were concentrated by filtration, differential centrifugation, and exclusion chromatography. Next, the nonionic detergent, Nonidet P-40, was used to extract the ecto-ATPDase from vesiculosomal membranes, and the solubilized enzyme was further purified by ion exchange (DEAE-Bio-Gel) and lentil-lectin-Sepharose 4B chromatography. In the final stage, immunoaffinity chromatography was utilized to obtain purified ecto-ATPDase. More than 25,000-fold purification was achieved. Specific activity of the purified enzyme was greater than 800 micronol/min/mg of protein with MgATP as the substrate, the highest ever reported for an ATPDase. The enzyme also hydrolyzed other nucleoside triphosphates in the presence of magnesium at similar rates and CaATP and MgADP at lower rates. The molecular mass of the purified glycoprotein was 80 kDa as determined by SDS-polyacrylamide gel electrophoresis and Western blot analysis. Based on its enzymatic properties, the relationship of the chicken oviduct ecto-ATPDase with other reported ATPDases and ecto-ATPases is discussed.
Subject(s)
Adenosine Triphosphatases/isolation & purification , Adenosine Triphosphatases/metabolism , Oviducts/enzymology , Animals , Antibodies, Monoclonal , Blotting, Western , Chickens , Chromatography, Affinity , Chromatography, Ion Exchange , Electrophoresis, Polyacrylamide Gel , Enzyme Inhibitors/pharmacology , Female , Glycoproteins/isolation & purification , Glycoproteins/metabolism , Kinetics , Mice , Substrate SpecificityABSTRACT
8-Azidoadenosine triphosphate labeled in the alpha or gamma position with 32P was used as a photoaffinity reagent for identifying ATP binding sites on the external surface of intact rat brain synaptosomes. As revealed by autoradiography of sodium dodecyl sulfate-polyacrylamide gel electrophoretic patterns. UV irradiation of intact synaptosomes in the presence of the above radioactive compounds at 5-10 microM resulted in the formation of several major radioactive conjugates with approximate molecular masses of 29, 45/46, 58, and 93 kDa. Minor bands of 20, 39, 52/54, 82/84, 120, and 140 kDa were also consistently labeled in these experiments. The possibility that labeling of these proteins was due to the presence of contaminating subcellular particles or intrasynaptosomal proteins was excluded. The major 8-azidoadenosine [alpha-32P]triphosphate-labeled protein complex of approximately 45/46 kDa was resolved into several subbands that are labeled differently depending on the type of divalent cations added to the photoaffinity reaction. In the presence of magnesium only, the major labeled band appeared at 45 kDa. With calcium, two additional subbands (43 and 46 kDa) could be distinguished. In the presence of 1 mM EDTA, a band at 44 kDa was labeled within this ATP-binding complex. The labeling pattern of the subbands of this 45/46-kDa complex is consistent with these bands being extracellular ATP-binding proteins on the surface of the synaptosome.
Subject(s)
Carrier Proteins/metabolism , Synaptic Membranes/metabolism , Adenosine Triphosphate/analogs & derivatives , Adenosine Triphosphate/pharmacology , Affinity Labels , Animals , Autoradiography , Azides/pharmacology , Binding Sites , Electrophoresis, Polyacrylamide Gel , Phosphorylation , Rats , Rats, Sprague-Dawley , Synaptosomes/metabolismABSTRACT
Using the radionuclide 65Zn, we have demonstrated the direct binding of zinc to purified rhodopsin. 65Zn is eluted with detergent-solubilized rhodopsin from concanavalin A columns and remains bound to the visual pigment through a subsequent gel-filtration step. Zinc binding to purified disc membranes is highly specific and, of the ions tested, copper is the best competitor. Equilibrium-dialysis experiments indicate that zinc binding to detergent-solubilized forms of rhodopsin may increase on bleaching the photopigment. These results may have important implications for studies that indicate that zinc plays a role in retinal degeneration and normal photoreceptor physiology.
Subject(s)
Rhodopsin/metabolism , Rod Cell Outer Segment/metabolism , Zinc/metabolism , Animals , Calcium/pharmacology , Cations, Divalent , Cattle , Chromatography, Affinity , Chromatography, Gel , Darkness , Electrophoresis, Polyacrylamide Gel , Kinetics , Light , Rhodopsin/isolation & purification , Zinc RadioisotopesABSTRACT
Intact nerve endings (synaptosomes) have been isolated from spiking and non-spiking temporal cortex and hippocampus samples from 14 patients immediately after temporal lobectomy for intractable epilepsy. Synaptosomes were also prepared from frozen brain samples of humans with no known neurological diseases. Four adenosine triphosphatase (ATP)-metabolizing enzymes (ecto-ATPase, ecto-adenylate kinase, Na+,K(+)-ATPase and Ca2+,Mg2(+)-ATPase) were assayed in the synaptosomal fractions from the most spiking temporal cortex area (including focus) as well as from various regions of the hippocampus, and compared with enzyme activities of the least spiking or non-spiking temporal cortex of the same patient. Enzyme activities of the epileptic brain samples were also compared with values measured in the corresponding regions of normal brains. Ecto-ATPase activities of epileptic temporal cortex were decreased (approximately 30%) in both comparisons. In contrast to these findings, a substantially increased (in some cases 300%) ecto-ATPase activity was observed in the posterior part of epileptic hippocampus. We suggest that the higher than normal ecto-ATPase activity in this particular hippocampal region is related to the presence of granule cells and their efferent (or afferent) synaptic connections. The synaptosomal ecto-adenylate kinase showed alterations opposite to the changes found for the ecto-ATPase. The intrasynaptosomal ATPase (Na+,K(+)- and Ca2+,Mg2(+)-) were decreased in the epileptic hippocampus-, but not in the temporal cortex samples, in relation to the corresponding normal enzyme activity values. These complex alterations in synaptosomal ATP-metabolizing enzyme activities may be important elements of seizure development and maintenance in human temporal lobe epilepsy.
Subject(s)
Adenosine Triphosphatases/metabolism , Epilepsies, Partial/enzymology , Hippocampus/enzymology , Synaptosomes/enzymology , Temporal Lobe/enzymology , Adenylate Kinase/metabolism , Adult , Ca(2+) Mg(2+)-ATPase/metabolism , Calcium-Transporting ATPases/metabolism , Epilepsies, Partial/surgery , Female , Hippocampus/surgery , Humans , Male , Mitochondria/enzymology , Organ Specificity , Reference Values , Sodium-Potassium-Exchanging ATPase/metabolism , Temporal Lobe/surgeryABSTRACT
Adenylate kinase activity (ATP:AMP-phosphotransferase; EC 2.7.4.3) was studied in various subcellular fractions of rat brain tissues. Because of the presence of other adenosine nucleotide-utilizing enzymes, adenylate kinase activity was assayed in both the forward and reverse directions by using coupled enzyme systems and by using a specific adenylate kinase inhibitor, P1,P5-di(adenosine-5') pentaphosphate. As expected, the highest specific adenylate kinase activity (2.89 mumol/min/mg of protein) was detected in the cytosolic brain fraction. However, substantial enzyme activity (0.68 mumol/min/mg) was also found in the intact synaptosomal fraction isolated on Percoll/sucrose gradients. The increased specific enzyme activity of purified synaptosomes and the differences found between the kinetic parameters of the membrane-bound and cytosolic enzyme forms suggest that the synaptosomal adenylate kinase activity cannot be attributed to the small amount of contaminating cytosol present in our preparations. The adenylate kinase enzyme adhered to purified synaptic plasma membranes and was not released by washings with isoosmotic sucrose medium. The facts that the adenylate kinase enzyme activity could be measured in intact synaptosomal preparations and that both its substrates and its inhibitors do not cross intact plasma membranes support the possibility that the synaptosomal adenylate kinase is an ecto-enzyme.
Subject(s)
Adenylate Kinase/metabolism , Brain/enzymology , Phosphotransferases/metabolism , Synaptosomes/enzymology , Animals , Cell Fractionation/methods , Cell Nucleus/enzymology , Centrifugation, Density Gradient/methods , Cytosol/enzymology , Kinetics , Microsomes/enzymology , Mitochondria/enzymology , Rats , Synaptosomes/ultrastructureABSTRACT
The rod photoreceptor outer segment maintains a remarkable morphology. Two of the proteins which have been implicated in the maintenance of this structure are the 240 kDa spectrin-like protein, and the 220 kDa glycoprotein often referred to as the rim protein. We have probed rat rod outer segment proteins with light-activated (azido-labeled) radioactive nucleotides and found a nucleotide binding site(s) on the rim protein which has a preference for guanine nucleotides. Binding to this site is stimulated by the divalent cations zinc, manganese and magnesium, but not calcium. This site is under investigation and may play a role in stabilizing protein structure.
Subject(s)
ATP-Binding Cassette Transporters , Azides/metabolism , Eye Proteins/metabolism , Membrane Proteins/metabolism , Photoreceptor Cells/metabolism , Rod Cell Outer Segment/metabolism , Adenosine Triphosphate/analogs & derivatives , Adenosine Triphosphate/metabolism , Animals , Binding Sites , Electrophoresis, Polyacrylamide Gel , Guanosine Triphosphate/analogs & derivatives , Guanosine Triphosphate/metabolism , Magnesium/pharmacology , Manganese/pharmacology , Molecular Weight , Rats , Zinc/pharmacologyABSTRACT
ATP has important roles in the vertebrate rod outer segment (ROS) physiological response to light. One of them is the quench of light-activated cGMP-phosphodiesterase activity. How ATP quenches PDE is not established; however, leading hypotheses favor the intervention of a 48-kDa ATP-binding protein and/or an ATP-utilizing rhodopsin kinase in this reaction. We have investigated the binding of [alpha 32P]8-azido-ATP to rat ROS proteins in the presence and absence of various divalent cations and competitive nucleotides. An event we have detected which might further clarify the role of ATP in PDE inactivation is a zinc-induced binding of azido-ATP to rhodopsin. Manganese is also effective in inducing this binding, while magnesium and calcium are not. The azido-ATP binding is eliminated by the addition of ATP, but not GTP, UTP, cGMP, or cAMP. A nucleotide-binding site on the rim protein is also suggested from these studies.
Subject(s)
Adenosine Triphosphate/analogs & derivatives , Azides/metabolism , Eye Proteins/metabolism , GTP-Binding Proteins/metabolism , Heterotrimeric GTP-Binding Proteins , Membrane Proteins/metabolism , Photoreceptor Cells/metabolism , Rod Cell Outer Segment/metabolism , Adenosine Triphosphate/metabolism , Animals , Binding, Competitive , Electrophoresis, Polyacrylamide Gel , Manganese/pharmacology , Phosphodiesterase Inhibitors , Protein Binding , Rats , Rhodopsin/metabolism , Zinc/pharmacologyABSTRACT
Intact synaptosomes isolated from mammalian brain tissues (rat, mouse, gerbil, and human) have an ATP hydrolyzing enzyme activity on their external surface. The synaptosomal ecto-ATPase(s) possesses characteristics consistent with those that have been described for ecto-ATPases of various other cell types. The enzyme has a high affinity for ATP (the apparent Km values are in the range of 2-5 X 10(-5) M), and is apparently stimulated equally well by either Mg2+ or Ca2+ in the absence of any other cations. The apparent activation constant for both divalent cations is approximately 4 X 10(-4) M in all mammalian brain tissues studied. The involvement of a non-specific phosphatase in the hydrolysis of externally added ATP is excluded. ATP hydrolysis is maximal in the pH range 7.4-7.8 for both divalent cation-dependent ATPase activities. Dicyclohexylcarbodiimide, 2,4-dinitrophenol, trifluoperazine, chlorpromazine, and p-chloromercuribenzoate (50 microM) inhibit the ecto-ATPase, whereas ouabain (1 mM) and oligomycin (3.5 micrograms X mg-1 protein) show little or no inhibition of this enzyme activity. Inhibitor data suggest that the Mg2+- and Ca2+-dependent ecto-ATPase may represent two different enzymes on the surface of synaptosomes.