Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 110
Filter
1.
Orv Hetil ; 164(41): 1616-1627, 2023 Oct 15.
Article in Hungarian | MEDLINE | ID: mdl-37987695

ABSTRACT

The growth hormone-insulin-like growth factor-1 (GH-IGF-1) axis plays a crucial role in maintaining the normal function of the cardiovascular system. Results of the last decades demonstrated that GH-IGF-1 takes part in regulating peripheral resistance and contributes to preserving physiological cardiac mass and left ventricular function. Vasculoprotective functions of the GH-IGF-1 axis are believed to counteract atherosclerosis. Unlike in childhood, when GH-deficiency results in growth retardation, GH deficiency does not cause specific symptoms in adults. Adult growth hormone deficiency (AGHD) is characterized by a clustering of cardiometabolic risk factors resulting in a clinical picture similar to the metabolic syndrome. Besides visceral obesity, dyslipidemia and insulin resistance, novel cardiovascular risk factors, such as chronic low-grade inflammation, oxidative stress and prothrombotic state have also been reported in AGHD and may contribute to the increased cardiometabolic risk. Based on a growing body of evidence, long-term GH-replacement improves lipid profile significantly and has a favorable impact on body composition, endothelial function, left ventricular mass as well as the novel, non-traditional cardiometabolic risk factors. Increased mortality associated with the disease is now considered to be multicausal and as such cannot be solely attributed to the GH-deficiency. The etiology of GH-deficiency, treatment of the underlying pathology as well as the inadequate treatment of coexisting hormonal deficiencies might also be responsible for the increased mortality. Nevertheless, in hypopituitarism, adequate replacement therapy including GH-substitution may result in a mortality that is comparable to the general population. Orv Hetil. 2023; 164(41): 1616-1627.


Subject(s)
Atherosclerosis , Cardiovascular System , Hypopituitarism , Adult , Humans , Insulin-Like Growth Factor I , Hypopituitarism/complications , Hypopituitarism/drug therapy , Growth Hormone
2.
Orv Hetil ; 163(12): 463-472, 2022 03 20.
Article in Hungarian | MEDLINE | ID: mdl-35306479

ABSTRACT

Összefoglaló. Bevezetés: A pajzsmirigy-alulmuködés gyakori betegség. Kezelésében a levotiroxin (LT4)-pótlás a szokásos eljárás, mely tabletta vagy gélkapszula formájában áll rendelkezésre Magyarországon. A nemzetközi trendeknek megfeleloen az esetek korai felismerése miatt már a kevésbé kifejezett hormonális eltérések idején elindul a kezelés. Az endokrinológusok hypothyreosiskezelési szokásaival kapcsolatban Magyarországon és Európában felmérés eddig nem történt. Célkituzés: A THESIS (Treatment of Hypothyroidism in Europe by Specialists: an International Survey) célja, hogy felmérjük az európai és közte jelen munkánkban a magyar endokrinológusok hypothyreosiskezelési szokásait és az LT4 esetleges alkalmazását pajzsmirigy-muködészavarral nem járó állapotokban. Módszer: A Magyar Endokrinológiai és Anyagcsere Társaság (MEAT) tagjainak e-mailben meghívót küldtünk az online kérdoíves vizsgálathoz. Eredmények: 165 magyar endokrinológus válaszai alapján végeztük az elemzést. A válaszadók többsége, 99,4%-uk elso kezelésként LT4-pótlást alkalmaz. Az LT4 + LT3 kombinációt elsosorban olyan betegeknél alkalmazzák, akik LT4 szedése mellett euthyreoid hormonértékek ellenére hypothyreosisra jellemzo tüneteket mutatnak (36,1%). Euthyreoid hormonértékek mellett, magas antitestszint és infertilitás esetén 60,3% megfontolná LT4 indítását, amit evidenciák jelenleg nem indokolnak. Számos kórállapot befolyásolja az LT4 felszívódását, ezekben az esetekben a magyar endokrinológusok 66,4%-a preferálja a lágy kapszula alkalmazását, jobb eredményt várva a gyógyszerformák közötti váltástól. Következtetés: A pajzsmirigy-alulmuködés kezelésében a magyar endokrinológusok elsodlegesen az LT4-et választják. Az LT4 + LT3 kombinált alkalmazását a pajzsmirigy-stimuláló hormon normális szintjének elérése után perzisztáló hypothyreosisos tünetek esetén fontolják meg. Az újabb gyógyszerformákat a többség preferálja, ha az LT4 hagyományos tablettás formájának alacsonyabb biohasznosulása várható. Orv Hetil. 2022; 163(12): 463-472. INTRODUCTION: Hypothyroidism has a high prevalence in the adult population. Levothyroxine (LT4) supplementation is considered to be the gold-standard treatment method. In Hungary, LT4 tablets and soft gel capsules are the available formulations. Similarly to the international trends, hypothyroidism is earlier recognised, leading to early LT4 supplementation. Up till now, there has been no survey on the treatment of hypothyroidism among Hungarian endocrinologists. OBJECTIVE: THESIS (Treatment of Hypothyroidism in Europe by Specialists: an International Survey) had been conducted to assess treatment preferences among European endocrinologists. Here we report the results on the use of thyroid hormones in hypothyroid patients and euthyroid individuals in Hungary. METHOD: An e-mail invitation to participate, containing the link to the online survey was sent to members of the Hungarian Society for Endocrinology and Metabolism. RESULTS: There were 165 responses with full demographics which were included in the analysis. By the majority (99.4%) of them, LT4 was the first treatment of choice. LT4 + LT3 combination was considered an option in patients with persistent symptoms despite biochemical euthyroidism while on LT4 (36,1%). In euthyroid individuals, 60.3% of the respondents would consider starting LT4 in euthyroid infertile women with high antibody levels, which is hardly supported by evidence. In the presence of comorbidities and interfering medications which may hinder LT4 absorption, 66.4% of Hungarian endocrinologist anticipate significant improvement after switching from tablets to soft gel capsules. CONCLUSION: The treatment of choice for hypothyroidism is LT4 in Hungary. Combination therapy with LT4 + LT3 was considered for patients with persistent symptoms. In the presence of diseases and interfering medications affecting bioavailability, a high number of Hungarian endocrinologists prefer the new LT4 formulation. The administration of LT4 in euthyroid conditions awaits explanation and calls for intensive discussions at local conferences and courses. Orv Hetil. 2022; 163(12): 463-472.


Subject(s)
Hypothyroidism , Infertility, Female , Physicians , Adult , Female , Humans , Hungary , Hypothyroidism/drug therapy , Surveys and Questionnaires , Thyroxine/therapeutic use
3.
Scand J Rheumatol ; 51(4): 279-283, 2022 07.
Article in English | MEDLINE | ID: mdl-35023427

ABSTRACT

OBJECTIVE: Studies indicate that caffeine uptake may be a risk factor for rheumatoid arthritis (RA), but a definitive link between caffeine consumption and RA has not been established. This study aimed to investigate the interplay between caffeine, adenosine receptor A2a, and interferon-γ (IFN-γ) production in CD4+ T cells from RA patients. METHOD: Peripheral blood mononuclear cells were obtained from the peripheral blood of healthy individuals and patients with RA. CD4+ T cells were isolated using the magnetic activated cell sorting technique and cultured in vitro with caffeine or mock control. In addition, adenosine was used as a competitive inhibitor of caffeine. After 48 h, expression of IFN-γ and interleukin-17 (IL-17) was analysed by flow cytometry. Ex vivo expression levels of adenosine receptor A2a were also assessed. RESULTS: Caffeine promoted IFN-γ production in Th1 cells in vitro. Significantly higher concentrations of caffeine were required to increase IFN-γ levels in Th1 cells from healthy individuals compared to Th1 cells from patients with RA. Moreover, ex vivo levels of adenosine receptor A2a expression on CD4+ T cells were significantly higher in RA than in healthy individuals. Caffeine-driven IFN-γ production was completely reversed by adenosine, a competitive agonist of adenosine receptor A2a. In contrast to IFN-γ, production of IL-17 was not affected by caffeine. CONCLUSION: Caffeine promotes IFN-γ production in Th1 cells from RA patients in vitro by competitive inhibition of adenosine receptor A2a. Excessive coffee consumption could contribute to T-cell activation and inflammation in RA.


Subject(s)
Adenosine A2 Receptor Antagonists , Arthritis, Rheumatoid , Caffeine , Interferon-gamma , Th1 Cells , Adenosine A2 Receptor Antagonists/pharmacology , Arthritis, Rheumatoid/immunology , Caffeine/pharmacology , Humans , Interferon-gamma/metabolism , Leukocytes, Mononuclear/metabolism , Th1 Cells/metabolism , Th2 Cells/metabolism
5.
Orv Hetil ; 161(2): 75-79, 2020 Jan.
Article in Hungarian | MEDLINE | ID: mdl-31902232

ABSTRACT

The authors present the case of a multiplex endocrine neoplasia type 2A (MEN2A). The 55-year-old woman underwent detailed examinations for abdominal complaints. Bilateral adrenal masses and thyroid nodular goiter were found. Based on metanephrine excretion and MIBG imaging, bilateral phaeochromocytomas were diagnosed. The thyroid nodules were confirmed by thyroidectomy as bilateral medullary thyroid carcinoma. Asymptomatic primary hyperparathyroidism was also detected. Laparoscopic adrenalectomy and parathyroid adenoma removal were performed. Based on family history and the characteristic clinical presentation, MEN2A syndrome was confirmed by genetic testing. During genetic screening of first-degree relatives, the patient's 25-year-old daughter was shown to be a gene carrier. Preventive thyroidectomy was performed and histology proved multifocal medullary thyroid cancer. In addition to the importance of genetic testing, the authors emphasize the guideline-based, but individualized approach to patients with suspected MEN2A syndrome. Orv Hetil. 2020; 161(2): 75-79.


Subject(s)
Adrenal Gland Neoplasms/surgery , Adrenalectomy , Multiple Endocrine Neoplasia Type 2a/diagnosis , Pheochromocytoma , Thyroid Neoplasms/diagnostic imaging , Thyroid Nodule/surgery , 3-Iodobenzylguanidine , Adrenal Gland Neoplasms/pathology , Adult , Female , Goiter, Nodular , Humans , Metanephrine , Middle Aged , Multiple Endocrine Neoplasia Type 2a/genetics , Parathyroid Neoplasms , Ribosomal Proteins , Thyroid Neoplasms/genetics , Thyroid Nodule/pathology , Thyroidectomy
6.
Orv Hetil ; 160(16): 636-637, 2019 04.
Article in Hungarian | MEDLINE | ID: mdl-30983398
7.
Neuropeptides ; 68: 75-83, 2018 Apr.
Article in English | MEDLINE | ID: mdl-29477253

ABSTRACT

RANKL signalling known to be important for the control of bone mass, has recently also been implicated in the brain to control thermoregulation, however, it is not known which neuronal pathways are involved and whether other aspects of energy homeostasis are also affected. Here we show that selective deletion of RANK from NPY neurons down-regulated NPY mRNA expression in the hypothalamus. While comprehensive phenotyping of germline-induced NPY neuron specific RANK deficient mice revealed no significant changes in physical or metabolic parameters, adult onset deletion of RANK from NPY neurons led to a significant increase in fat mass and a decrease in whole body bone mineral content and bone mineral density. Intriguingly, when these conditional knockout mice were placed on a high fat diet, body weight and fat mass did not differ to control mice. However, they were able to significantly increase their bone mass to match their increased body weight, an ability that was lacking in control mice. Taken together, results from this study demonstrate that RANK signalling in NPY neurons is involved in modulating NPY levels and through that matching bone mass to body weight.


Subject(s)
Bone and Bones/anatomy & histology , Bone and Bones/metabolism , Hypothalamus/metabolism , Neurons/metabolism , Neuropeptide Y/metabolism , Receptor Activator of Nuclear Factor-kappa B/metabolism , Animals , Bone Density , Diet, High-Fat , Eating , Energy Metabolism , Male , Mice, Knockout , RNA, Messenger/metabolism , Signal Transduction
8.
Graefes Arch Clin Exp Ophthalmol ; 255(1): 25-30, 2017 Jan.
Article in English | MEDLINE | ID: mdl-27377657

ABSTRACT

PURPOSE: Diabetic retinopathy is one of the leading causes of blindness. There are several risk factors, such as the duration of diabetes or glycemic control of the patient; however, several biochemical factors also alter the process. Our aim was to investigate the role of soluble E-selectin in the formation of diabetic retinopathy. PATIENTS AND METHODS: Fifty-seven patients (37 female and 20 male, aged 61.71 ± 12.31 years) and 14 healthy control subjects (ten female and four male, aged 63.06 ± 10.46 years) were enrolled in the study. We measured the soluble E-selectin level in the plasma of patients by ELISA. All patients underwent careful ophthalmological examination, including ophthalmoscopy and color fundus photography, while diabetic retinopathy grading was performed in line with the 2012 classification of the American Academy of Ophthalmology (AAO). RESULTS: The soluble E-selectin level was significantly higher in patients with diabetes compared to controls (32.95 ng/ml vs. 26.55 ng/ml, p = 0.03). Dividing patients into groups by the presence of retinopathy, the E-selectin level was also significantly higher in the retinopathy group (p < 0.05). When we examined diabetic patients by the severity of retinopathy (groups A, B, and C, by the guidelines of the AAO), however, we did not find any significant difference in soluble E-selectin levels, although it tended to be higher in group B. CONCLUSIONS: An elevated E-selectin level can play a role in the development of diabetic retinopathy, but it does not seem to alter disease severity. However, glycemic control and the reduction of cardiovascular risk factors may also alter the level of E-selectin that might play a role in the prevention of diabetic retinopathy.


Subject(s)
Diabetes Mellitus, Type 2/complications , Diabetic Retinopathy/blood , E-Selectin/blood , Aged , Biomarkers/blood , Diabetes Mellitus, Type 2/blood , Diabetic Retinopathy/etiology , Disease Progression , Enzyme-Linked Immunosorbent Assay , Female , Follow-Up Studies , Glycated Hemoglobin/metabolism , Humans , Male , Middle Aged , Retrospective Studies , Risk Factors
9.
Acta Endocrinol (Buchar) ; 13(4): 425-430, 2017.
Article in English | MEDLINE | ID: mdl-31149211

ABSTRACT

CONTEXT: Epidemiological data have shown that obesity increases the risk of developing colorectal cancer and also an increased body mass index (BMI) is associated with a worse prognosis. Bevacizumab based systemic therapy, an antiVEGF targeted therapy, is an important treatment option for metastatic colorectal cancer (mCRC) patients. Obesity is associated with high level of vascular endothelial growth factor (VEGF), that might provoke resistance to antiVEGF monoclonal antibody. OBJECTIVE: To evaluate the efficacy in terms of progression free survival (PFS) and overall survival (OS) of bevacizumab systemic therapy in patients with mCRC. DESIGN: Retrospective cohort, single center study. SUBJECTS AND METHODS: Between January 2007 and December 2012, 112 patients with mCRC, who followed bevacizumab based systemic therapy in the "Ion Chiricuta" Oncology Institute in Cluj-Napoca, were included in our analysis. RESULTS: Values of BMI ≥ or <27 kg/sqm was found that PFS is statistically significant superior in patients with BMI<27 kg/sqm (n=77) than in those with BMI ≥ 27 kg/sqm (n=35), 24 months versus 17.9 months (p = 0.04). Five years OS was not influenced by the BMI, 35% vs 30% (p=0.29). In patients with liver metastases with values of BMI ≥ 27 kg/sqm have PFS lower than patients with a BMI <27 kg/sqm, 17.5 months versus 24.5 months (p = 0.02). Five years OS was not influenced by the BMI, 39% (BMI <27 kg/sqm) vs. 22% (BMI ≥ 27 kg/sqm) (p = 0.09). CONCLUSIONS: This study demonstrated the negative influence of BMI on both PFS on the entire sample of patients and in patients with liver metastases only, BMI cut-off value proved to be 27 kg/square meter and shows that the BMI may be an important prognostic factor with a high clinical relevance in patients with mCRC.

10.
Chem Commun (Camb) ; 52(75): 11267-11269, 2016 Sep 13.
Article in English | MEDLINE | ID: mdl-27711396

ABSTRACT

Composite materials made of cellulose fibers and spin crossover micro-particles were investigated by magnetic measurements and dynamic mechanical analysis (DMA). The storage modulus of the cellulose handsheet (0.6 GPa at room T) is significantly enhanced in the composite (1.7 GPa). The latter also displays a reversible increase of ca. 10% when switching the magnetic spin state of the particles from the low spin (LS) to the high spin (HS) form. Around the spin transition temperature a loss modulus peak is also observed, highlighting the strong viscoelastic coupling between the particles and the cellulose matrix. These results pave the way for the development of a novel family of actuator materials based on spin crossover-polymer composites.

11.
Gene Ther ; 23(10): 718-726, 2016 10.
Article in English | MEDLINE | ID: mdl-27356950

ABSTRACT

Adoptive cell therapy with chimeric antigen receptor (CAR)-modified T cells showed remarkable therapeutic efficacy in the treatment of leukaemia/lymphoma. However, the application to a variety of cancer entities is often constricted by the non-availability of a single chain antibody (scFv), which is usually the targeting domain in a CAR, while antibodies in the natural format are often available. To overcome the limitation, we designed a CAR that uses an antibody in its natural configuration for binding. Such CAR consists of two chains, the immunoglobulin light and heavy chain with their constant regions, whereby the heavy chain is anchored to the membrane and linked to an intracellular signalling domain for T-cell activation. The two chains form a stable heterodimer, a so-called dual chain CAR (dcCAR), and bind with high affinity and in a specific manner to their cognate antigen. By specific binding, the dcCAR activates engineered T cells for the release of pro-inflammatory cytokines and for target cell lysis. We provide evidence by three examples that the dcCAR format is universally applicable and thereby broadens the CAR cell therapy towards a larger variety of targets for which an scFv antibody is not available.


Subject(s)
Immunotherapy, Adoptive/methods , Lymphocyte Activation , Receptors, Antigen, T-Cell/genetics , Animals , Antibody Affinity , Cell Line, Tumor , Cells, Cultured , HEK293 Cells , Humans , Mice , Receptors, Antigen, T-Cell/chemistry , Receptors, Antigen, T-Cell/immunology , Single-Chain Antibodies/genetics , Single-Chain Antibodies/immunology
12.
Orv Hetil ; 157(8): 310-5, 2016 Feb 21.
Article in Hungarian | MEDLINE | ID: mdl-26876268

ABSTRACT

Graves' orbitopathy is the extrathyroidal manifestation of Graves' disease, which is the most common cause of exophthalmos. As eye symptoms usually coincide with the development of thyrotoxicosis, the diagnosis of the disease is rarely difficult. The aim of the authors was to summarize the differential diagnosis of Graves' orbitopathy based on literature review and presentation of their own four problematic cases on this topic. They conclude that symptoms similar to endocrine orbitopathy are present in other disorders. Endocrinologists need to be aware of these other conditions to avoid treatment failures.


Subject(s)
Adrenal Cortex Hormones/therapeutic use , Eye Neoplasms/diagnosis , Graves Ophthalmopathy/etiology , Hypergammaglobulinemia/diagnosis , Immunoglobulin G/blood , Inflammation/diagnosis , Leukemia, Lymphocytic, Chronic, B-Cell/diagnosis , Orbit/pathology , Thyrotoxicosis/diagnosis , Adrenal Cortex Hormones/administration & dosage , Adult , Aged , Diagnosis, Differential , Diplopia/etiology , Eye Neoplasms/complications , Female , Graves Ophthalmopathy/drug therapy , Humans , Hypergammaglobulinemia/complications , Inflammation/complications , Leukemia, Lymphocytic, Chronic, B-Cell/complications , Magnetic Resonance Imaging , Male , Middle Aged , Orbital Diseases/diagnosis , Thyrotoxicosis/complications , Treatment Outcome
13.
Graefes Arch Clin Exp Ophthalmol ; 253(9): 1581-6, 2015 Sep.
Article in English | MEDLINE | ID: mdl-25956998

ABSTRACT

PURPOSE: We measured vascular endothelial growth factor (VEGF) levels in tear fluid and serum in patients with retinal vein occlusion (RVO). PATIENTS AND METHODS: Eight patients with RVO due to secondary macular oedema were examined. VEGF levels were measured by enzyme-linked immunosorbent assay. All patients had a full ophthalmic examination (visual acuity, slit lamp biomicroscopy, perimetry, and fluorescein angiography). Central retinal thickness (CRT) was examined using optical coherence tomography (OCT). Tear and serum samples were collected and examinations were performed at diagnosis and 1 and 4 weeks later. RESULTS: VEGF levels in the tears of RVO eyes were significantly higher than in fellow eyes at diagnosis and after both 1 and 4 weeks (paired t test, p1 = 0.01, p2 = 0.02, p3 = 0.006). We found a weak but significant positive correlation between VEGF levels in tear fluid and serum of patients with RVO (r = 0.21), while this correlation tended to be stronger between the fellow eyes and serum levels (r = 0.33). CONCLUSION: To the best of our knowledge, we are the first to report an increased level of VEGF in the tear fluid of patients with RVO. Alterations of VEGF levels in tears may be useful for determining stages of RVO. This non-invasive and objective method may also be helpful for estimating the severity of macular oedema and efficacy of treatment.


Subject(s)
Eye Proteins/metabolism , Retinal Vein Occlusion/metabolism , Tears/metabolism , Vascular Endothelial Growth Factor A/metabolism , Aged , Enzyme-Linked Immunosorbent Assay , Female , Fluorescein Angiography , Humans , Macular Edema/complications , Macular Edema/diagnosis , Macular Edema/metabolism , Male , Microscopy, Acoustic , Middle Aged , Pilot Projects , Retinal Vein Occlusion/diagnosis , Retinal Vein Occlusion/etiology , Visual Acuity/physiology , Visual Field Tests
14.
Bratisl Lek Listy ; 116(2): 79-82, 2015.
Article in English | MEDLINE | ID: mdl-25665470

ABSTRACT

OBJECTIVES: Prostate cancer (PCa) represents one of the most complicated human tumors and, like many others malignancies, arises from progressive genetic and epigenetic alterations. Among all recognized epigenetic alterations, aberrant DNA methylation (hypo- and hypermethylation) is the most important and the best characterized change in PCa. BACKGROUND: We analyzed GSTP1, APC and RASSF1 gene promoter hypermethylation in urine DNA of ten previously non-treated prostate-diseased patients. METHODS: For the purpose, the quantitative real-time methylation specific PCR (MSP) with primers designed for amplification of methylated bisulfite-converted human DNA, followed by melting procedure, was currently optimized. RESULTS: GSTP1 gene promoter hypermethylation was detected in 2 and 1 out of 5 patients with biopsy-confirmed PCa using the primers covering the 3´ and 5´ CpG regions of the promoter, respectively. The APC gene promoter hypermethylation was found in neither of PCa or non-PCa patients and the RASSFI gene promoter hypermethylation was found in some non-PCa and not in all PCa patients. CONCLUSIONS: Our results suggest that GSTP1 gene promoter hypermethylation can be detected in urine DNA of PCa patients with real-time MSP followed by melting. This enables evaluation of its potential as a useful biomarker in the diagnosis and prognosis of PCa (Tab. 1, Fig. 1, Ref. 9).


Subject(s)
Biomarkers, Tumor/urine , DNA Methylation/genetics , DNA, Neoplasm/urine , Glutathione S-Transferase pi/genetics , Promoter Regions, Genetic , Prostatic Neoplasms/genetics , Prostatic Neoplasms/urine , Adenomatous Polyposis Coli Protein/genetics , Aged , Genes, APC , Genetic Markers/genetics , Humans , Male , Middle Aged , Prostatic Neoplasms/diagnosis , Tumor Suppressor Proteins/genetics
15.
Orv Hetil ; 155(33): 1295-300, 2014 Aug 17.
Article in Hungarian | MEDLINE | ID: mdl-25109914

ABSTRACT

Graves' orbitopathy is the most common extrathyroidal manifestation of Graves' disease. Up to now, curative treatment modalities for the most severe sight-threatening cases have not been developed. Here the authors summarize the treatment protocol of Graves' orbitopathy and review novel therapeutic options. They review the literature on this topic and present their own clinical experience. The authors point out that anti-CD20 antibody could positively influence the clinical course of Graves' orbitopathy. Selenium is efficient in mild cases. Further prospective investigations are warranted.


Subject(s)
Antibodies, Monoclonal, Murine-Derived/therapeutic use , Antibodies, Monoclonal/therapeutic use , Antigens, CD20/immunology , Graves Ophthalmopathy/therapy , Immunoglobulin G/therapeutic use , Immunologic Factors/therapeutic use , Receptors, Tumor Necrosis Factor/therapeutic use , Tumor Necrosis Factor-alpha/immunology , Etanercept , Graves Ophthalmopathy/immunology , Humans , Infliximab , Rituximab , Selenium/therapeutic use
16.
Radiat Prot Dosimetry ; 159(1-4): 46-51, 2014 Jun.
Article in English | MEDLINE | ID: mdl-24757174

ABSTRACT

This study evaluates both the effects of physical restraint and use of candidate biomarkers in a CD2F1 male mouse partial-body irradiation model for biological dosimetry diagnostic assays. Mice were irradiated (6-Gy, 250-kVp X ray) to 3/3rd (total body), 2/3rd (gut and torso), 1/3rd (gut only) and 0/3rd (sham) of total body. Blood was sampled for haematology and blood plasma proteomic biomarkers at 1 and 2 d after exposure. Increases in the body fraction exposed showed progressive decreases in lymphocyte counts and increases in the neutrophil-to-lymphocyte ratios with no significant differences in the neutrophil and platelet counts. The radioresponse for plasma biomarker Flt3L showed proportional increases; however, G-CSF and SAA levels exhibited dramatic and non-proportional increases in levels. Physical restraint at 1 d post-exposure increased lymphocyte counts and SAA, decreased neutrophil-to-lymphocyte ratio and Flt3L and showed no effects on neutrophil and platelet counts or G-CSF.


Subject(s)
Biomarkers/analysis , Blood Proteins/analysis , Proteome/analysis , Proteome/radiation effects , Radiometry , Animals , Biological Assay , Gamma Rays , Lymphocytes/metabolism , Lymphocytes/radiation effects , Male , Mice , Neutrophils/metabolism , Neutrophils/radiation effects , Proteomics/methods , Radiation Dosage
17.
Strahlenther Onkol ; 190(4): 405-7, 2014 Apr.
Article in English | MEDLINE | ID: mdl-24429480

ABSTRACT

BACKGROUND AND PURPOSE: This study aimed to determine factors allowing the prediction of extracranial metastases in patients presenting with brain metastases at the first diagnosis of cancer. MATERIALS AND METHODS: Data from 659 patients with brain metastases upon first diagnosis of cancer were retrospectively analyzed. The parameters age, gender, Karnofsky performance score (KPS), primary tumor type and number of brain metastases were compared between 359 patients with extracranial metastases and 300 patients without extracranial metastases. Additional analyses were performed for patients with the most unfavorable and those with the most favorable characteristics. RESULTS: The comparison of patients with versus without extracranial metastases revealed significant differences between the groups in terms of KPS (p < 0.001) and number of brain metastases (p < 0.001). Of the study patients, 113 had both most unfavorable characteristics, i.e. KPS ≤ 50 and ≥ 4 brain metastases. The sensitivity for identifying patients with extracranial metastases was 82 %; specificity was 51 %. A total of 50 patients had KPS ≥ 90 and only one brain metastasis. The sensitivity for identifying patients without extracranial metastases was 86 %; specificity was 58 %. CONCLUSION: The combination of KPS and the number of brain metastases can help to predict the presence or absence of extracranial metastases.


Subject(s)
Brain Neoplasms/diagnosis , Brain Neoplasms/secondary , Carcinoma/diagnosis , Carcinoma/secondary , Proportional Hazards Models , Aged , Brain Neoplasms/mortality , Carcinoma/mortality , Female , Germany/epidemiology , Humans , Incidence , Male , Middle Aged , Prognosis , Reproducibility of Results , Risk Assessment , Sensitivity and Specificity , Survival Rate
18.
Strahlenther Onkol ; 190(1): 64-7, 2014 Jan.
Article in English | MEDLINE | ID: mdl-24104871

ABSTRACT

BACKGROUND AND PURPOSE: This study investigated the potential prognostic value of the number of involved extracranial organs in patients with brain metastasis from non-small cell lung cancer (NSCLC). MATERIAL AND METHODS: A total of 472 patients who received whole-brain radiotherapy (WBRT) alone with 5 × 4 Gy or 10 × 3 Gy for brain metastasis from NSCLC were included in this retrospective study. In addition to the number of involved extracranial organs, 6 further potential prognostic factors were investigated including WBRT regimen, age, gender, Karnofsky Performance Score (KPS), number of brain metastases, and the interval from cancer diagnosis to WBRT. Subgroup analyses were performed for patients with metastatic involvement of one (lung vs. bone vs. other metastasis) and two (lung + bone vs. lung+lymph nodes vs. other combinations) extracranial organs. RESULTS: The survival rates at 6 months of the patients with involvement of 0, 1, 2, 3, and ≥ 4 extracranial organs were 52, 27, 17, 4, and 14%, respectively (p<0.001). On multivariate analysis, the number of involved extracranial organs remained significant (risk ratio 1.32; 95% confidence interval 1.19-1.46; p<0.001). Age <65 years (p=0.004), KPS ≥ 70 (p<0.001), and only 1-3 brain metastases (p=0.022) were also significantly associated with survival in the multivariate analysis. In the separate analyses of patients with involvement of one and two extracranial organs, survival was not significantly different based on the pattern of extracranial organ involvement. CONCLUSION: The number of involved extracranial organs is an independent prognostic factor of survival in patients with brain metastasis from NSCLC, irrespective of the pattern of extracranial organ involvement.


Subject(s)
Brain Neoplasms , Carcinoma, Non-Small-Cell Lung , Lung Neoplasms/mortality , Radiotherapy, Conformal/mortality , Survival Rate , Aged , Aged, 80 and over , Brain Neoplasms/mortality , Brain Neoplasms/radiotherapy , Brain Neoplasms/secondary , Carcinoma, Non-Small-Cell Lung/radiotherapy , Carcinoma, Non-Small-Cell Lung/secondary , Carcinoma, Non-Small-Cell Lung/therapy , Comorbidity , Female , Germany/epidemiology , Humans , Lung Neoplasms/therapy , Male , Middle Aged , Prognosis , Retrospective Studies , Risk Factors
19.
Vet Comp Oncol ; 12(3): 205-14, 2014 Sep.
Article in English | MEDLINE | ID: mdl-22947252

ABSTRACT

The aim of this work was to determine levels of carcinoembryonic antigen (CEA) and cancer antigen (CA 15-3) in the blood serum of 45 bitches. A modified procedure was used to determine the CEA and CA 15-3 markers with the human kits using the radioimmunoassay method. Samples collected from extirpated tumour of mammary glands were histologically processed and classified as per WHO guidelines. The average age of animals with tumour was 10.00 ± 2.2 years; for healthy bitches average age was 4.2 ± 3.2 years. Values of CEA and CA 15-3 were considered positive, if they exceeded 0.23 ng mL(-1) and 7 IU mL(-1) , respectively. Average levels of CEA in the tumour group were 0.25 ± 0.06 versus 0.20 ± 0.03 in healthy bitches (P = 0.0001). The average CA 15-3 value in bitches with tumour was 8.58 ± 1.27 versus 5.14 ± 1.34 in healthy animals (P < 0.0001).


Subject(s)
Carcinoembryonic Antigen/metabolism , Dog Diseases/metabolism , Mammary Neoplasms, Animal/metabolism , Mucin-1/metabolism , Animals , Biomarkers, Tumor/genetics , Biomarkers, Tumor/metabolism , Carcinoembryonic Antigen/genetics , Dog Diseases/genetics , Dogs , Female , Gene Expression Regulation, Neoplastic/physiology , Mammary Neoplasms, Animal/genetics , Mucin-1/genetics
20.
J BUON ; 18(4): 989-95, 2013.
Article in English | MEDLINE | ID: mdl-24344028

ABSTRACT

PURPOSE: The aim of this study was to analyze the characteristics of patients with rectal cancer operated with a microscopic positive margin (R1) and thus avoid these situations or adapt treatment in these particular cases. METHODS: We reviewed all the pathology data of resected specimens from patients with rectal or recto-sigmoid cancer operated with curative intent at the Institute of Oncology "Prof. Dr. Ion Chiricuta" between 2000-2011 (763 patients in 12 years) and the pathology files of patients from other institutions referred for adjuvant treatment to our hospital (318 patients). We included patients with anterior resection, Hartmann's procedure and abdomino-perineal resection, but we excluded patients with local excision and patients with R2/R1 at first, but R0 after re-resection (56 patients). We have identified 31 patients with R1, but had to exclude one case from analysis because this patient was lost to follow-up. RESULTS: With surgery alone the local relapse (LR) was unavoidable. In the neoadjuvant chemoradiation (CRT) group 85.7% of the patients did not develop LR despite of R1. In the adjuvant CRT cohort 50% of the patients were LR-free at 2 years after conventional radiotherapy (p<0.01). CONCLUSION: Based on these results it is concluded that a clear resection margin is extremely important for the local control of rectal cancer, because it cannot be always compensated by adjuvant CRT. In R1 cases neoadjuvant CRT seems to offer better prognosis than adjuvant CRT. To avoid R1 and its consequences a good quality control of total mesorectal excision (TME) is needed and CRT should be done before and not after surgery. R1 after primary surgery needs to be compensated by re-resection if possible, otherwise probably high dose radiotherapy with chemotherapy is needed.


Subject(s)
Adenocarcinoma/surgery , Rectal Neoplasms/surgery , Adenocarcinoma/mortality , Adenocarcinoma/secondary , Adult , Aged , Chemoradiotherapy, Adjuvant , Chi-Square Distribution , Disease-Free Survival , Female , Humans , Kaplan-Meier Estimate , Male , Middle Aged , Neoadjuvant Therapy , Neoplasm Recurrence, Local , Neoplasm, Residual , Radiotherapy, Adjuvant , Rectal Neoplasms/mortality , Rectal Neoplasms/pathology , Retrospective Studies , Risk Factors , Romania , Time Factors , Treatment Outcome , Young Adult
SELECTION OF CITATIONS
SEARCH DETAIL
...