[Application of lectins for determination of antibodies to glycoproteins of leukemia virus in bovine blood serum]. / Vykorystannia lektyniv dlia vyiavlennia antytil do hlikoproteïniv virusu leikozu v syrovattsi krovi velykoï rohatoï khudoby.

The possibility of using different lectins for the determination of level of specific antibodies to leukosis virus virus in bovine blood serum whit the help of the surface plasmon resonance was investigated. The modification of transducer surface with lectins provides for immobilisation of glycosilated proteins of leukaemia virus. It allows to make selective determination of antibodies to glicosilated protein, the quantity of which more high in blood serum of ill animals in the comparison with the immunised ones. Moreover, the treatment of surface with FGA-P and PLA lectins increases the sensitivity of immune sensor to the specific antibodies in the comparison with the non-treated one.

[Express diagnostics of bovine leucosis by immune sensor based on surface plasmon resonance]. / Ekspresna diahnostyka leikozu velykoï rohatoï khudoby za dopomohoiu imunnoho sensora na osnovi poverkhnevoho plazmonnoho rezonansu.

An immune sensor based on the surface plasmon resonance (SPR) was developed for express diagnostics of bovine leucosis. The sensor was used for detection of the level of antibodies against bovine leukaemia virus (BLV) in the blood serum. The industrially manufactured BLV antigen for screening test in the agar gel immunodiffusion (AGID) required the additional purification in order to be used in immune sensor analysis. It was shown that immune sensor analysis was more sensitive, rapid and simple in comparison with the traditional AGID test. It was stated that the developed immune sensor was capable to be used for performance of bovine leucosis screening at the farms and the minimal dilution of the serum should be 1:500.