1.
Biomed Chromatogr
; 3(6): 246-50, 1989 Nov.
Article
in English
| MEDLINE
| ID: mdl-2482788
ABSTRACT
A simple and precise method was developed for the separation of nucleosides including modified nucleosides and oligonucleotides. Nineteen kinds of nucleosides were completely separated by HPLC using an ODS column (TSK-gel ODS 80TM) and aqueous mobile phases. The RNA molecule was digested by base restrictive RNase (RNase A, RNase T1) and the digests were separated chromatographically into each oligonucleotide. The nucleoside composition of an oligonucleotide was then determined by this analytical system. It is thus possible to fit the oligonucleotide in the original RNA molecule by using modified bases as markers. The reaction site of quinacrine mustard for tRNA(Phe) (from yeast) could be determined by this analytical system.
Subject(s)
Chromatography, High Pressure Liquid , RNA/analysis , Ribonucleosides/isolation & purification , Base Sequence , Binding Sites , Molecular Sequence Data , Oligoribonucleotides/isolation & purification , Quinacrine Mustard , RNA, Fungal/analysis , RNA, Transfer, Phe/analysis , RNA, Transfer, Phe/metabolism , Ribonuclease T1/metabolism , Ribonuclease, Pancreatic/metabolism , Single-Strand Specific DNA and RNA Endonucleases/metabolism
2.
Jpn J Med Sci Biol
; 20(6): 447-60, 1967 Dec.
Article
in English
| MEDLINE
| ID: mdl-4968085
