ABSTRACT
OBJECTIVE: Metastasis-associated protein 1 (MTA1) has been associated with poor prognosis in several carcinomas. Recent investigation has found that in different tumors, MTA1 protein significantly correlates with tumor angiogenesis, suggesting that MTA1 may be a possible angiogenesis-promoting molecule in malignant tumors. Thus, the current study was performed to determine the role of MTA1 protein in the biological behavior of oral squamous cell carcinoma and its relation with tumor angiogenesis. MATERIAL AND METHOD: In this study, 44 oral squamous cell carcinomas and 15 normal epitheliums were reviewed by IHC staining for MTA1 and CD105. RESULTS: Frequency of MTA1 expression in SCCs was recorded as 97.7%, which was significantly higher than that of the control group (33.3%). Mean percentage of MTA1 expression in oral squamous cell carcinomas was 76.88 ± 25.33% which was significantly higher than that of the control group (22.81 ± 10.83). Our data showed a correlation between MTA1 expression with lymph node metastasis, tumor size and, stage. Evaluation of the correlation between MTA1 protein expression and micro vessel density showed that high micro vessel density was detected more frequently in tumors with MTA1 protein overexpression than in those without overexpression. CONCLUSION: In the present study, high expression of the MTA1 protein was seen in oral squamous cell carcinoma, and was closely associated with tumor progression and increased tumor angiogenesis. The findings may indicate that MTA1 protein has clinical potentials as a useful indicator of progressive phenotype, a promising prognostic predictor to identify patients with poor prognosis and may be a potential novel therapeutic target of anti-angiogenesis for patients with oral squamous cell carcinoma.
Subject(s)
Biomarkers, Tumor/analysis , Carcinoma, Squamous Cell/chemistry , Histone Deacetylases/analysis , Mouth Neoplasms/chemistry , Neovascularization, Pathologic , Repressor Proteins/analysis , Adult , Aged , Aged, 80 and over , Antigens, CD/analysis , Carcinoma, Squamous Cell/blood supply , Carcinoma, Squamous Cell/secondary , Case-Control Studies , Cross-Sectional Studies , Endoglin , Female , Humans , Immunohistochemistry , Lymphatic Metastasis , Male , Microvessels/pathology , Middle Aged , Mouth Neoplasms/blood supply , Mouth Neoplasms/pathology , Neoplasm Invasiveness , Neoplasm Staging , Receptors, Cell Surface/analysis , Trans-Activators , Tumor Burden , Up-RegulationABSTRACT
BACKGROUND: Proliferation markers widely have been used to diagnose and determine the behaviour and prognosis of benign and malignant tumours. Minichromosome maintenance 3 (MCM3) is a novel proliferation marker. The aim of this study was to evaluate and compare MCM3 with Ki-67 in diagnosis of salivary gland tumours. MATERIALS AND METHODS: In this retrospective study, immunohistochemical expression of MCM3 and Ki-67 was evaluated in 15 pleomorphic adenomas (PA), 17 mucoepidermoid carcinomas (MEC) and 18 adenoid cystic carcinomas (ADCC) . Labeling indices (LIs) for the two markers were calculated and compared. RESULTS: MCM3 and Ki-67 LIs were significantly higher in MEC and ADCC compared to PA. The LI of MCM3 was significantly higher than that of Ki-67 in MEC and PA. There was no significant difference between the two markers in ADCC. A cut-off point of 8% with 74.3% sensitivity and 93.3% specificity for MCM3 was obtained to discern between benign and malignant tumors. CONCLUSIONS: These results suggest that MCM3 might be a useful proliferation marker for differential diagnosis and recognition of clinical behavior of salivary gland tumors.
Subject(s)
Adenocarcinoma/diagnosis , Adenoma, Pleomorphic/diagnosis , Carcinoma, Adenoid Cystic/diagnosis , Carcinoma, Mucoepidermoid/diagnosis , Minichromosome Maintenance Complex Component 3/metabolism , Salivary Gland Neoplasms/diagnosis , Salivary Glands/pathology , Adenocarcinoma/metabolism , Adenoma, Pleomorphic/metabolism , Adult , Biomarkers, Tumor/metabolism , Carcinoma, Adenoid Cystic/metabolism , Carcinoma, Mucoepidermoid/metabolism , Case-Control Studies , Cell Proliferation , Female , Follow-Up Studies , Humans , Immunoenzyme Techniques , Ki-67 Antigen/metabolism , Male , Middle Aged , Neoplasm Staging , Prognosis , Salivary Gland Neoplasms/metabolismABSTRACT
OBJECTIVE: To investigate the association between CD105 and tumor cell proliferation in salivary gland tumors. METHODS: In this study, 59 samples of salivary tumors from Khalili Hospital archive, including 20 cases of pleomorphic adenoma (PA), 20 cases of mucoepidermoid carcinoma (MEC) and 19 cases of adenoid cystic carcinoma, as well as 10 cases of normal salivary gland tissue, were reviewed by immunohistochemistry (IHC) for CD105 and Ki67 staining. RESULTS: CD105 positive vessels were absent in normal salivary gland tissue in the vicinity of tumors (51.6% of all tumors were positive). There was a statistically significant difference in frequency of CD105 staining between PA and malignant tumors and between four groups of different lesions (p<0.000) being highest in MEC. Intratumoral microvessel density was also elevated in malignant neoplasms (2.61 ± 3.1) as compared to PA (0.46 ± 0.6). Normal salivary glands did not express Ki67. There was a statistically significant difference in frequency and percentage of Ki67 immunoreactivity in malignant neoplasms (86.5% and 10.7 ± 10.8 respectively) compared to PA (50% and 0.78 ± 0.2) and among the four groups values were highest in MEC (p<0.000). CONCLUSION: n this study, it was observed a higher rate of angiogenesis and cellular proliferation was noted in malignant tumors compared to benign tumors, but no correlation was observed between these two markers.
