Long-lasting effects of incentives and social preference: A public goods experiment.

This paper addresses the question of the effectiveness and permanence of temporary incentives to contribute to a public good. Using a common experimental framework, we investigate the effects of a recommendation that takes the form of an exhortative message to contribute, a monetary punishment and a non-monetary reward to sustain high levels of contributions. In particular, we shed light on the differential impact these mechanisms have on heterogeneous types of agents. The results show that all three incentives increase contributions compared to a pre-phase where there is no incentive. Monetary sanctions lead to the highest contributions, but a sudden drop in contributions is observed once the incentive to punish is removed. On the contrary, Recommendation leads to the lowest contributions but maintains a long-lasting impact in the Post-policy phase. In particular, it makes free-riders increase their contribution over time in the post-incentive phase. Finally, non-monetary reward backfires against those who are weakly conditional cooperators. Our findings emphasize the importance of designing and maintaining incentives not only for free-riders, but for strong and weak conditional cooperators as well, depending on characteristics of the incentives.

Total hydroxyl radical reactivity measurements in a suburban area during AQUAS-Tsukuba campaign in 2017.

Missing hydroxyl radical (OH) reactivity from unknown/unmeasured trace species empirically accounts for 10%-30% of total OH reactivity and may cause significant uncertainty regarding estimation of photochemical ozone production. Thus, it is essential to unveil the missing OH reactivity for developing an effective ozone mitigation strategy. In this study, we conducted simultaneous observations of total OH reactivity and 54 reactive trace species in a suburban area as part of the Air QUAlity Study (AQUAS)-Tsukuba campaign for the summer of 2017 to gain in-depth insight into total OH reactivity in an area that experienced relatively high contributions of secondary pollutants. The campaign identified on average 35.3% of missing OH reactivity among total OH reactivity (12.9 s-1). In general, ozone-production potential estimation categorized ozone formation in this area as volatile organic compound (VOC)-limited conditions, and missing OH reactivity may increase ozone production potential 40% on average if considered. Our results suggest the importance of photochemical processes of both AVOCs and BVOCs for the production of missing OH reactivity and that we may underestimate the importance of reducing precursors in approach to suppressing ozone production if we ignore the contribution of their photochemical products.

Relative and Absolute Sensitivity Analysis on Ozone Production in Tsukuba, a City in Japan.

The change in the ozone production rate on reducing its precursors, namely, ozone production sensitivity, is important information for developing a strategy to reduce ozone. We expanded a conventional sensitivity analysis theory by including peroxy radical loss by uptake onto particle surfaces in the aim of examining their potential impact. We also propose a new concept of absolute sensitivity that enables us to evaluate the quantitative effectiveness of precursor reduction toward mitigating ozone production over a given period and area. This study applies the theory to observations in Tsukuba, a city in Japan. The relative sensitivity analysis shows that ozone production was more sensitive to volatile organic compounds (VOCs) in the morning and evening, and it became more sensitive to NOx in the afternoon. NO depletion was a main trigger in this sensitivity regime transition. The absolute sensitivity analysis indicates that the VOC-sensitive period in the morning determines the total ozone production sensitivity in a day. While particles did not have significant impact on regime classification in Tsukuba, they have a potential to decrease the mitigating effect of VOC reduction on ozone production and to moderate the enhancement effect of NOx reduction depending upon uptake coefficients. A further study will benefit from a combination with an observation-constrained box model simulation or chemical transport modeling system, which may provide sensitivity analysis over a large spatial and temporal range.

Kinetics Study of OH Uptake onto Deliquesced NaCl Particles by Combining Laser Photolysis and Laser-Induced Fluorescence.

Despite the role of hydroxyl radical (OH) uptake onto sea-salt particles as a daytime chlorine source, affecting the chemical processes in the marine boundary layer, its uptake coefficient has not yet been confirmed by direct measurement methods. This study reports the application of a combination technique of laser flash photolysis generation and laser-induced fluorescence detection for the direct kinetic measurement of OH uptake onto deliquesced NaCl particles. The uptake coefficient was not constant and inversely depended on the initial OH concentration, indicating that the first uptake step is Langmuir-type adsorption. The resistance model, including surface processes, well reproduced the observed uptake coefficient. The model predicted an uptake coefficient for the atmospheric relevant OH concentration within the range from 0.77 to 0.95. Such values may lead to emissions of Cl2 higher than those predicted in previous studies based on other values. Hence, the proposed value may provide more reliable estimations of ozone formation, oxidation of volatile organic compounds, secondary organic aerosol formation, and lifetime of methane and elemental mercury in the marine boundary layer.

Inflammatory cytokine-mediated induction of serine racemase in atopic dermatitis.

Serine racemase (SR) is an enzyme that catalyses the synthesis of d-serine, an endogenous coagonist for N-methyl-D-aspartate (NMDA)-type glutamate receptor in the central nervous system. Our previous study demonstrated that SR was expressed in the epidermis of wild-type (WT) mice but not in SR knockout (KO) mice. In addition, SR immune-reactivity was only found in the granular and cornified layers of the epidermis in WT mice. These findings suggested that SR is involved in the differentiation of epidermal keratinocytes and the formation of the skin barrier. However, its role in skin barrier dysfunction such as atopic dermatitis (AD) remains elusive. AD is a chronic inflammatory disease of skin, and the clinical presentation of AD has been reported to be occasionally associated with psychological factors. Therefore, this study examined the content of d-serine in stratum corneum in AD patients and healthy controls using a tape-stripping method. Skin samples were collected from the cheek and upper arm skin of AD patient's lesion and healthy individuals. The d-serine content was significantly increased in the involved skin of AD in comparison with healthy individuals. An immunohistochemical analysis also revealed an increased SR expression in the epidermis of AD patients. Furthermore, the SR expression in cultured human keratinocytes was significantly increased by the stimulation with tumour necrosis factor -α or macrophage migration inhibitory factor. Taken together, these findings suggest that d-serine expressed particularly strongly in AD lesional skin and that the SR expression in the keratinocytes is linked to inflammatory cytokines.

The protective role of DJ-1 in ultraviolet-induced damage of human skin: DJ-1 levels in the stratum corneum as an indicator of antioxidative defense.

DJ-1 is a multifunctional protein associated with Parkinson's disease and plays a significant role in protecting nerve cells from oxidative stress. DJ-1 is expressed in the skin, although its function there is unknown. In this study, we investigated DJ-1 function in keratinocytes. DJ-1 was induced by H2O2 exposure and UV irradiation in keratinocytes. DJ-1 knockdown with small interfering RNA (siRNA) increased reactive oxygen species (ROS) and lactate dehydrogenase (LDH) release after UVB irradiation, suggesting that DJ-1 reduces ROS and might protect skin cells from UV damage in vitro. To investigate the in vivo role of DJ-1 in the skin, we determined DJ-1 levels in human stratum corneum samples obtained by the tape-stripping method. DJ-1 levels in the stratum corneum (scDJ-1) correlated with total antioxidant capacity. We also examined the effect of scDJ-1 on changes in skin after UVB irradiation. DJ-1 was elevated in SC from the upper arm 1 to 2 weeks after UVB irradiation. One day after UVB irradiation, L* (brightness) and a* (redness) values, indicators of skin color, were altered regardless of scDJ-1 expression. However, these values recovered more quickly in subjects with high scDJ-1 expression than in those with low scDJ-1 expression. These data suggest that DJ-1 in skin plays a significant role in protection against UV radiation and oxidative stress, and that DJ-1 levels in the SC might be an indicator of antioxidative defense against UV-induced damage.