Biodegradation Mechanism of Polystyrene by Mealworms (Tenebrio molitor) and Nutrients Influencing Their Growth.

A degradation mechanism of polystyrene (PS) in mealworms reared on expanded PS (EPS) was investigated by its decrease in molecular weight and change in chemical structure. A 33% decrease in molecular weight was observed for the digested PS in the frass after 1 week of feeding to mealworms. The FT-IR and py-GC/MS spectra of the digested PS showed radical oxidative reactions taking place in the mealworm body. The presence of hydroperoxide, alcohol and phenol groups was confirmed, and dimer fragments of styrene with quinone and phenol groups were obtained. The decrease in molecular weight and the alternation of benzene rings indicated that autoxidation and quinonization via phenolic intermediates occurred simultaneously in the mealworm body. The survival rate of mealworms reared on EPS was higher than that of starved worms, indicating that EPS was a nutrient source. However, no weight gain was observed in mealworms fed EPS alone. Comparison with the mixed diets with bran or urethane foams (PU) indicated that protein, phosphorus and magnesium components absent from EPS were required for mealworm growth.

Meta-learning, social cognition and consciousness in brains and machines.

The intersection between neuroscience and artificial intelligence (AI) research has created synergistic effects in both fields. While neuroscientific discoveries have inspired the development of AI architectures, new ideas and algorithms from AI research have produced new ways to study brain mechanisms. A well-known example is the case of reinforcement learning (RL), which has stimulated neuroscience research on how animals learn to adjust their behavior to maximize reward. In this review article, we cover recent collaborative work between the two fields in the context of meta-learning and its extension to social cognition and consciousness. Meta-learning refers to the ability to learn how to learn, such as learning to adjust hyperparameters of existing learning algorithms and how to use existing models and knowledge to efficiently solve new tasks. This meta-learning capability is important for making existing AI systems more adaptive and flexible to efficiently solve new tasks. Since this is one of the areas where there is a gap between human performance and current AI systems, successful collaboration should produce new ideas and progress. Starting from the role of RL algorithms in driving neuroscience, we discuss recent developments in deep RL applied to modeling prefrontal cortex functions. Even from a broader perspective, we discuss the similarities and differences between social cognition and meta-learning, and finally conclude with speculations on the potential links between intelligence as endowed by model-based RL and consciousness. For future work we highlight data efficiency, autonomy and intrinsic motivation as key research areas for advancing both fields.

Computing Social Value Conversion in the Human Brain.

Social signals play powerful roles in shaping self-oriented reward valuation and decision making. These signals activate social and valuation/decision areas, but the core computation for their integration into the self-oriented decision machinery remains unclear. Here, we study how a fundamental social signal, social value (others' reward value), is converted into self-oriented decision making in the human brain. Using behavioral analysis, modeling, and neuroimaging, we show three-stage processing of social value conversion from the offer to the effective value and then to the final decision value. First, a value of others' bonus on offer, called offered value, was encoded uniquely in the right temporoparietal junction (rTPJ) and also in the left dorsolateral prefrontal cortex (ldlPFC), which is commonly activated by offered self-bonus value. The effective value, an intermediate value representing the effective influence of the offer on the decision, was represented in the right anterior insula (rAI), and the final decision value was encoded in the medial prefrontal cortex (mPFC). Second, using psychophysiological interaction and dynamic causal modeling analyses, we demonstrated three-stage feedforward processing from the rTPJ and ldPFC to the rAI and then from rAI to the mPFC. Further, we showed that these characteristics of social conversion underlie distinct sociobehavioral phenotypes. We demonstrate that the variability in the conversion underlies the difference between prosocial and selfish subjects, as seen from the differential strength of the rAI and ldlPFC coupling to the mPFC responses, respectively. Together, these findings identified fundamental neural computation processes for social value conversion underlying complex social decision making behaviors.SIGNIFICANCE STATEMENT In daily life, we make decisions based on self-interest, but also in consideration for others' status. These social influences modulate valuation and decision signals in the brain, suggesting a fundamental process called value conversion that translates social information into self-referenced decisions. However, little is known about the conversion process and its underlying brain mechanisms. We investigated value conversion using human fMRI with computational modeling and found three essential stages in a progressive brain circuit from social to empathic and decision areas. Interestingly, the brain mechanism of conversion differed between prosocial and individualistic subjects. These findings reveal how the brain processes and merges social information into the elemental flow of self-interested decision making.

Computational Neuroscience: Mathematical and Statistical Perspectives.

Mathematical and statistical models have played important roles in neuroscience, especially by describing the electrical activity of neurons recorded individually, or collectively across large networks. As the field moves forward rapidly, new challenges are emerging. For maximal effectiveness, those working to advance computational neuroscience will need to appreciate and exploit the complementary strengths of mechanistic theory and the statistical paradigm.

Temporal and Rate Coding for Discrete Event Sequences in the Hippocampus.

Although the hippocampus is critical to episodic memory, neuronal representations supporting this role, especially relating to nonspatial information, remain elusive. Here, we investigated rate and temporal coding of hippocampal CA1 neurons in rats performing a cue-combination task that requires the integration of sequentially provided sound and odor cues. The majority of CA1 neurons displayed sensory cue-, combination-, or choice-specific (simply, "event"-specific) elevated discharge activities, which were sustained throughout the event period. These event cells underwent transient theta phase precession at event onset, followed by sustained phase locking to the early theta phases. As a result of this unique single neuron behavior, the theta sequences of CA1 cell assemblies of the event sequences had discrete representations. These results help to update the conceptual framework for space encoding toward a more general model of episodic event representations in the hippocampus.

Kinematic analysis of stair climbing in rotating platform cruciate-retaining and posterior-stabilized mobile-bearing total knee arthroplasties.

INTRODUCTION: The aim of our study was to compare and contrast the effects of two types of mobile-bearing total knee arthroplasties (TKA), namely, the cruciate-retaining (CR) and posterior-stabilized (PS) TKAs, on clinical outcomes and in vivo kinematics during stair climbing. MATERIALS AND METHODS: The Press-Fit Condylar Sigma rotating platform was used for both CR and PS TKAs. Patient-reported outcomes were assessed using the 2011 Knee Society Score. Quadriceps muscle strength was evaluated by isokinetic dynamometry. In vivo kinematics were evaluated using periodic sagittal plane radiographic images obtained during stair climbing to quantify anteroposterior (AP) tibiofemoral translation, implant flexion and axial rotation angles using image-matching techniques. Outcomes were evaluated in 20 TKAs, which had been undergone with clinical success, including ten knees with CR types and ten knees with PS types. RESULTS: There were no significant differences between the CR and PS TKA groups (p > 0.05) in isometric extensor torque (1.0 ± 0.2 and 1.1 ± 0.6 N m/kg, respectively) or patient-reported score for stair climbing function (4.0 ± 0.5 and 3.8 ± 0.9, respectively). Both types of TKAs showed stable AP translation in the mid range of knee flexion and paradoxical translation in the low range of flexion, with limited rotation, during stair climbing. There were no significant differences between the CR and PS TKA groups (P > 0.05) in anterior translation from 80° to 40° of knee flexion (4.2 ± 1.2 and 3.5 ± 1.6 mm, respectively), posterior translation from 40° to 10° of knee flexion (2.3 ± 1.9 and 2.0 ± 1.5 mm, respectively), and total external rotation (2.8° ± 4.9° and 0.5° ± 5.0°, respectively). CONCLUSIONS: Both CR and PS types of rotating platform mobile-bearing TKAs provided reproducible knee joint kinematics during stair climbing and equivalent clinical outcomes. LEVEL OF EVIDENCE: IV.

In Vivo Kinematics of Healthy and Osteoarthritic Knees During Stepping Using Density-Based Image-Matching Techniques.

The purpose of this study was to investigate in vivo kinematics in healthy and osteoarthritic (OA) knees during stepping using image-matching techniques. Six healthy volunteers and 14 patients with a medial OA knee before undergoing total knee arthroplasty performed stepping under periodic anteroposterior radiograph images. We analyzed the three-dimensional kinematic parameters of knee joints using radiograph images and CT-derived digitally reconstructed radiographs. The average extension/flexion angle ranged 6°/53° and 16°/44° in healthy and OA knees, with significant difference in extension (P = .02). The average varus angle was -2° and 6° in healthy and OA knees, with a significant difference (P = .03). OA knees showed 1.7° of significantly larger varus thrust (P = .04) and 4.2 mm of significantly smaller posterior femoral rollback (P = .04) compared with healthy knees. Coronal limb alignment in OA knees significantly correlated with varus thrust (R2 = .36, P = .02) and medial shift of the femur (R2 = .34, P = .03). Both normal and OA knees showed no transverse plane instability, including anteroposterior, mediolateral directions, or axial rotation. In conclusion, OA knees demonstrated different kinematics during stepping from normal knees: less knee extension, larger varus thrust, less posterior translation, and larger medial shift.

Two-dimensional planning can result in internal rotation of the femoral component in total knee arthroplasty.

PURPOSE: The first purpose of this study was to compare the reproducibility of two-dimensional (2D) and three-dimensional (3D) measurements for preoperative planning of the femoral side in total knee arthroplasty (TKA). The second purpose was to evaluate the factors affecting the differences between the 2D and 3D measurements. METHODS: Two-dimensional and 3D measurements for preoperative planning of the femoral side in TKA were evaluated in 75 varus knees with osteoarthritis. The femoral valgus angle, defined as the angle between the mechanical and anatomical axes of the femur, and the clinical rotation angle and surgical rotation angle, defined by the angles between the posterior condylar line and the clinical or surgical transepicondylar axes, respectively, were analysed using 2D (radiographs and axial CT slices) and 3D (3D bone models reconstructed from CT images) measurements. RESULTS: For all variables, 3D measurements were more reliable and reproducible than 2D measurements. The medians and ranges of the clinical rotation angle and surgical rotation angle were 6.6° (-1.7° to 12.1°) and 2.3° (-2.5° to 8.6°) in 2D, and 7.1° (2.7° to 11.4°) and 3.0° (-2.0° to 7.5°) in 3D. Varus/valgus alteration of the CT scanning direction relative to the mechanical axis affected the difference in clinical rotation angles between 2D and 3D measurements. CONCLUSION: Significantly, smaller values of the clinical rotation angle and surgical rotation angle were obtained by 2D compared to 3D measurements, which could result in internal rotation of the femoral component even if the surgeon performs the bone cutting precisely. Regarding clinical relevance, first, this study confirmed the reliability of 3D measurements. Second, it underscored the risk of internal rotation of the femoral component when using 2D measurement, even with precise bone cutting technique. These results will help surgeons avoid malpositioning of the femoral component if 2D measurements are used for preoperative planning in TKA. LEVEL OF EVIDENCE: Prospective comparative study, Level Ш.

Distal femoral condyle is more internally rotated to the patellar tendon at 90° of flexion in normal knees.

BACKGROUND: The configuration of the distal surface of the femur would be more important in terms of the patellofemoral (PF) joint contact because the patella generally contacts with the distal surface of the femur in knee flexion. Some total knee arthroplasty (TKA) designs configurate medially prominent asymmetric femoral condyles. This difference in the design of distal femoral condyle may affect the PF joint congruity in knee flexion. Furthermore, some surgeons advocate a concept aligning the symmetric components parallel to the native joint inclination, not perpendicular to the mechanical axis. This concept would also make a difference on the PF joint congruity at the distal femur in knee flexion. However, no fundamental study has been reported on the PF congruity at the distal femur to discuss the theoretical priority of these concepts. The current study investigated the angular relationship between the tibial attachment of the patellar tendon and the distal surface of the femur at 90° of flexion in normal knees. METHODS: The open magnetic resonance images of 45 normal knees at 90° of flexion were used to measure the angles between the tibial attachment of the patellar tendon, the equatorial line of the patella, and the distal surface of femoral condyles. RESULTS: The distal surface of femoral condyles was internally rotated relative to the tibial attachment of the patellar tendon and the equatorial line of the patella in all the knees (8.2° ± 3.5° and 5.8° ± 2.5°, respectively), not parallel. CONCLUSIONS: Distal femoral condyle is internally rotated to the patellar tendon at 90° of flexion in normal knees. When the symmetric femoral component is aligned perpendicular to the femoral mechanical axis, the patellar tendon would be possibly more twisted than the condition in normal knees, and the deviation of the PF contact force on the patellar component might be caused. The configuration and alignment of the distal condyle of the femoral component can affect the PF joint congruity in knee flexion. In this respect, our results provide important information in considering designs and alignment in the distal femur of TKA and the PF joint congruity in knee flexion.

Rotational alignment of the tibial component affects the kinematic rotation of a weight-bearing knee after total knee arthroplasty.

PURPOSE: The purpose of this study is to elucidate how the rotational malalignment of prosthesis after total knee arthroplasty affects the rotational kinematics in a weight-bearing condition. METHODS: In this study of 18 knees replaced with the posterior stabilizing fixed-bearing system, which has a relatively low-restricting design, rotational angles between the femoral and tibial components and between the femur and tibia during stair climbing were evaluated in vivo in three dimensions using radiologically based image-matching techniques. Rotational alignments of the components were assessed by postoperative CT. The correlations between the rotational alignments and the rotational angles during stair climbing were evaluated. RESULTS: Rotational alignment of the tibial component significantly correlated with rotational angles between the components as well as between bones during stair climbing. Rotational malalignment of the tibial component toward internal rotation caused a rotational mismatch of the tibial component toward internal rotation relative to the femoral component in 0° extension and caused a rotational mismatch of the tibia (bone) toward external rotation relative to the femur (bone). The knee in which the tibial component was placed close to the AP axis of the tibia did not show any rotational mismatch between either components or bones. CONCLUSIONS: Rotational alignment of the tibial component affects the kinematic rotation of the replaced knee during a weight-bearing condition even though using a low-restricting designed surface, and the AP axis can be a reliable reference in determining rotational alignment for the tibial component.

Effect of Tibial Posterior Slope on Knee Kinematics, Quadriceps Force, and Patellofemoral Contact Force After Posterior-Stabilized Total Knee Arthroplasty.

We used a musculoskeletal model validated with in vivo data to evaluate the effect of tibial posterior slope on knee kinematics, quadriceps force, and patellofemoral contact force after posterior-stabilized total knee arthroplasty. The maximum quadriceps force and patellofemoral contact force decreased with increasing posterior slope. Anterior sliding of the tibial component and anterior impingement of the anterior aspect of the tibial post were observed with tibial posterior slopes of at least 5° and 10°, respectively. Increased tibial posterior slope contributes to improved exercise efficiency during knee extension, however excessive tibial posterior slope should be avoided to prevent knee instability. Based on our computer simulation we recommend tibial posterior slopes of less than 5° in posterior-stabilized total knee arthroplasty.

Continuous sagittal radiological evaluation of stair-climbing in cruciate-retaining and posterior-stabilized total knee arthroplasties using image-matching techniques.

In this study, we evaluated the in vivo kinematics of stair-climbing after posterior stabilized (PS) and cruciate retaining (CR) total knee arthroplasty (TKA) using radiographic-based image-matching techniques. Mid-flexion anteroposterior stability was demonstrated in all knees after CR TKA. However, paradoxical femoral translation at low flexion angles was seen in both designs. The post-cam mechanism did not function after PS TKA. Larger posterior tibial slope in PS TKA was linked to forward sliding of the femur at mid-flexion and unintended anterior tibial post impingement at knee extension. CR TKA is more sagittally stable in mid-flexion during stair climbing and attention must be given to minimize posterior tibial slope when using late cam-post engaging PS TKA designs.

Correlations between patient satisfaction and ability to perform daily activities after total knee arthroplasty: why aren't patients satisfied?

BACKGROUND: Patient satisfaction has become an important parameter for assessing overall outcomes after total knee arthroplasty (TKA). The level of difficulty in performing activities of daily life that affects overall patient satisfaction is unknown. We therefore evaluated the influence of difficulty in performing activities of daily life on patient satisfaction and expectations. METHODS: The 2011 Knee Society Knee Scoring System Questionnaire was mailed to patients who had undergone TKA with 375 patients completing and returning it. We evaluated the relationship between the ability to perform daily activities, as assessed via the questionnaire, and patient satisfaction and expectations of the same score in each patient using linear regression analysis. We also determined which activities affected patient satisfaction and expectations using multivariate linear regression analyses. RESULTS: All patient-derived functional activities correlated significantly with the patient satisfaction score. In particular, "climbing up or down a flight of stairs" followed by "getting into or out of a car," "moving laterally (stepping to the side)" and "walking and standing" correlated strongly with patient satisfaction by linear regression analysis and were revealed to have significant contributions to patient satisfaction by multivariate linear regression analysis. Regarding expectations, all patient-derived functional activities correlated significantly with the patient expectation score, although none of the correlation coefficients was very high. "Squatting," followed by "walking and standing," contributed to the patient expectation score by multivariate linear regression analysis. CONCLUSION: Activities related to walking and standing are some of the most basic movements and basic demands for patients. In addition, "climbing up or down a flight of stairs," "getting into and out of a car" and "squatting" are very important and distressing activities that significantly correlate with patient satisfaction after TKA.

Does knee stability in the coronal plane in extension affect function and outcome after total knee arthroplasty?

PURPOSE: The aim of the present study was to clarify whether varus-valgus laxities under static stress in extension, femoral condylar lift-off during walking, and patient-reported outcomes after total knee arthroplasty (TKA) were correlated with each other. METHODS: Ninety-four knees, which had undergone posterior-stabilized TKA, were analysed. The varus-valgus laxity during knee extension was measured using a stress radiograph. New Knee Society Score (KSS) questionnaires were mailed to all patients. Correlations between the values of stress radiographs and KSS were analysed. Additionally, continuous radiological images were taken of 15 patients while each walked on a treadmill to determine condylar lift-off from the tibial tray using a 3D-to-2D image-to-model registration technique. Correlations between the amount of lift-off and either the stress radiograph or the KSS were also analyzed. RESULTS: The mean angle measured was 5.9 ± 2.7° with varus stress and 5.0 ± 1.6° with valgus stress. The difference between them was 0.9 ± 2.8°. Varus-valgus laxities, or the differences between them, did not show any statistically significant correlation with either component of the KSS (p > 0.05). The average amount of femoral condylar lift-off during walking was 1.4 ± 0.8 mm (medial side) and 1.3 ± 0.6 mm (lateral side). The amount of lift-off did not correlate with either varus-valgus laxities or the KSS (p > 0.05). CONCLUSIONS: No correlations were found among varus-valgus laxities under static stress in extension, femoral condylar lift-off during walking, or patient-reported outcomes after well-aligned TKA. This study suggests that small variations in coronal laxities do not influence lift-off during walking and the patient-reported outcomes. LEVEL OF EVIDENCE: IV.

Transcription factor Mohawk controls tenogenic differentiation of bone marrow mesenchymal stem cells in vitro and in vivo.

Mohawk homeobox (MKX) has been demonstrated as a tendon/ligament specific transcription factor. The aim of this study was to investigate the role of MKX in ligament/tenogenic differentiation of bone marrow derived mesenchymal stem cells (BMMSCs). Human BMMSCs were treated with 50 ng/ml BMP-12 or transduced with MKX or scleraxis (SCX) adenoviral vector. Gene expression analysis was performed by quantitative reverse transcribed polymerase chain reaction (qRT-PCR). Rat BMMSCs were seeded in a collagen scaffold and transplanted into a rat Achilles tendon defect model. Tenogenesis related gene expressions and histological features were analyzed. BMP-12 induced tenogenesis in BMMSCs as indicated by increased COL1a1, TNXB, DCN and SCX mRNA, and MKX expression increased simultaneously. Rat BMMSCs enhanced defect repair and were still detectable 3 weeks after transplantation. Increased expressions of COL1a1, TNC and TNMD in vivo were also correlated with upregulated MKX. Adenoviral MKX promoted expression of COL1a1, TNXB, and TNMD in BMMSCs. This study demonstrated that MKX gene expression is enhanced during the tenogenic differentiation of BMMSCs in vitro and in vivo, and the adenoviral overexpression of MKX increases tendon extracellular matrix gene expression and protein production. Thus, MKX is a key factor for tenogenic differentiation of MSCs.

Dual reward prediction components yield Pavlovian sign- and goal-tracking.

Reinforcement learning (RL) has become a dominant paradigm for understanding animal behaviors and neural correlates of decision-making, in part because of its ability to explain Pavlovian conditioned behaviors and the role of midbrain dopamine activity as reward prediction error (RPE). However, recent experimental findings indicate that dopamine activity, contrary to the RL hypothesis, may not signal RPE and differs based on the type of Pavlovian response (e.g. sign- and goal-tracking responses). In this study, we address this discrepancy by introducing a new neural correlate for learning reward predictions; the correlate is called "cue-evoked reward". It refers to a recall of reward evoked by the cue that is learned through simple cue-reward associations. We introduce a temporal difference learning model, in which neural correlates of the cue itself and cue-evoked reward underlie learning of reward predictions. The animal's reward prediction supported by these two correlates is divided into sign and goal components respectively. We relate the sign and goal components to approach responses towards the cue (i.e. sign-tracking) and the food-tray (i.e. goal-tracking) respectively. We found a number of correspondences between simulated models and the experimental findings (i.e. behavior and neural responses). First, the development of modeled responses is consistent with those observed in the experimental task. Second, the model's RPEs were similar to dopamine activity in respective response groups. Finally, goal-tracking, but not sign-tracking, responses rapidly emerged when RPE was restored in the simulated models, similar to experiments with recovery from dopamine-antagonist. These results suggest two complementary neural correlates, corresponding to the cue and its evoked reward, form the basis for learning reward predictions in the sign- and goal-tracking rats.

Internal rotation of femoral component affects functional activities after TKA--survey with the 2011 Knee Society Score.

The 2011 Knee Society Knee Scoring System (KSS) was developed as a new patient-derived outcome measure to better characterize satisfaction, expectations, and physical activities after total knee arthroplasty. The rotational alignment of the femoral and tibial components was assessed with computed tomography in 75 patients (92 knees), and its effect on the scores of the KSS was evaluated. Internal rotation of the femoral component relative to the surgical epicondylar axis significantly decreased the score of functional activities and slightly decreased the score of satisfaction. Femoral component malrotation did not affect the scores of symptoms and expectations. Tibial component malrotation did not affect any of the scores of the 2011 KSS. Surgeons should avoid internal rotation of the femoral component to preserve functional activities.

Multiplexing signals in reinforcement learning with internal models and dopamine.

A fundamental challenge for computational and cognitive neuroscience is to understand how reward-based learning and decision-making are made and how accrued knowledge and internal models of the environment are incorporated. Remarkable progress has been made in the field, guided by the midbrain dopamine reward prediction error hypothesis and the underlying reinforcement learning framework, which does not involve internal models ('model-free'). Recent studies, however, have begun not only to address more complex decision-making processes that are integrated with model-free decision-making, but also to include internal models about environmental reward structures and the minds of other agents, including model-based reinforcement learning and using generalized prediction errors. Even dopamine, a classic model-free signal, may work as multiplexed signals using model-based information and contribute to representational learning of reward structure.

Improved design decreases wear in total knee arthroplasty with varus malalignment.

PURPOSE: Controversy still exists whether coronal malalignment would influence the long-term survival of total knee arthroplasty (TKA). The hypothesis was that an improved design of the articular surface of modern TKA would prevent the increase in contact stresses and thus decrease the wear even when the implant was placed in a varus position. Two different designs of TKA were compared biomechanically and clinically. METHODS: The patients whose prosthesis was initially placed in a varus alignment by the postoperative long-leg radiographs were selected. Seventeen knees using the NexGen LPS and 16 knees using the MG I were examined. Changes in postoperative alignment and the thickness of the polyethylene insert in a follow-up period of approximately 7 years were evaluated. Additionally, an in vitro biomechanical testing was conducted to measure the contact stresses and the contact area at the tibiofemoral joint of the NexGen LPS and the MG I components mounted on a servohydraulic testing device. RESULTS: Although the long-leg alignment did not change in NexGen LPS, the varus alignment significantly progressed in MG I. The thickness of polyethylene insert in MG I decreased a significantly greater amount compared with that in NexGen LPS. Biomechanical test showed that the NexGen LPS had a larger contact area and lower mean and peak contact stresses than the MG I significantly. CONCLUSION: These results suggest that comprehensive factors of modern prosthesis including improved implant designs could improve the durability of polyethylene insert and decrease implant failures due to component malalignment.