1.
J Biosci Bioeng
; 99(3): 293-5, 2005 Mar.
Article
in English
| MEDLINE
| ID: mdl-16233792
ABSTRACT
A new method based on a combination of reverse transcription polymerase chain reaction (RT-PCR) and a water-in-oil (W/O) emulsion was developed. Reverse transcription and initial thermal cycles were carried out in droplets of the W/O emulsion. Then, the droplets were united, followed by remaining secondary PCR cycles. This two-step method succeeded in detecting a single RNA molecule.