A modular and multi-functional purification strategy that enables a common framework for manufacturing scale integrated and continuous biomanufacturing.

Biopharmaceutical manufacture is transitioning from batch to integrated and continuous biomanufacturing (ICB). The common framework for most ICB, potentially enables a global biomanufacturing ecosystem utilizing modular and multi-function manufacturing equipment. Integrating unit operation hardware and software from multiple suppliers, complex supply chains enabled by multiple customized single-use flow paths, and large volume buffer production/storage make this ICB vision difficult to achieve with commercially available manufacturing equipment. Thus, we developed SymphonX™, a downstream processing skid with advanced buffer management capabilities, a single disposable generic flow path design that provides plug-and-play flexibility across all downstream unit operations and a single interface to reduce operational risk. Designed for multi-product and multi-process cGMP facilities, SymphonX™ can perform stand-alone batch processing or ICB. This study utilized an Apollo™ X CHO-DG44 mAb-expressing cell line in a steady-state perfusion bioreactor, harvesting product continuously with a cell retention device and connected SymphonX™ purification skids. The downstream process used the same chemistry (resins, buffer composition, membrane composition) as our historical batch processing platform, with SymphonX™ in-line conditioning and buffer concentrates. We used surge vessels between unit operations, single-column chromatography (protein A, cation and anion exchange) and two-tank batch virus inactivation. After the first polishing step (cation exchange), we continuously pooled product for 6 days. These 6 day pools were processed in batch-mode from anion exchange to bulk drug substance. This manufacturing scale proof-of-concept ICB produced 0.54 kg/day of drug substance with consistent product quality attributes and demonstrated successful bioburden control for unit-operations undergoing continuous operation.

What are the kinematic characteristics of the world champion couple in competitive ballroom dance during the waltz's spin movement?

To evaluate competitive ballroom dancing, one of the official events of the World Games, spin movements used by advanced dancers who ranked high in actual competition may be employed. The purpose of this study was to investigate the kinematic characteristics of spin movements in competitive ballroom dancing performed by world champion couples, especially holding posture and lower limb movements. A champion couple and 13 national-level competitive ballroom dancers as the control group participated in this study. An inertial measurement unit (IMU) system (MVN Link; Xsens, Netherlands) consisting of 17 IMUs, attached to feet, shanks, thighs, pelvis, sternum, head, upper arms, forearms, and hands of the dancers, was used to obtain three-dimensional kinematic data at 240 Hz. The overall trend was that the actual and normalized stride lengths of the champion male and female dancers tended to be longer than those of the male and female dancers in the control group. Further, large differences were observed in the pelvic and rib cage segments movements, and the relative angle of the rib cage segment to the pelvic segment. The champion male dancer started to move from the pelvic segment, whereas the champion female dancer and the national-level top male and female dancers started to move from the rib cage segment. During the spin movement, the champion male dancer was in a position where the rib cage segment was rotated to the left with respect to the pelvic segment, whereas the other dancers were in a position where the rib cage segment was rotated to the right. Although limited to technical aspects, these dance kinematic characteristics of the world champion couples and their differences from those of the control group dancers, will be helpful to competitive ballroom dancers and coaches in their daily practice.

Partnering Effects on Joint Motion Range and Step Length in the Competitive Waltz Dancers.

Competitive dance, also known as DanceSport, is one of the official sports of the World Games. The most salient characteristic of ballroom dance is the closed-hold position, during which the upper body segments of partner-dancers are linked. This study aimed to investigate partnering effects on joint motion ranges of the lower extremity and step lengths during the waltz in 13 national level competitive dance couples and a world champion couple. A Xsens MVN system was used to record movement at 240 Hz. Solo and pair conditions were examined. Compared with the highly skilled couples, the world champion couple demonstrated superior dance skills for generating the first step length in the pair condition of the waltz. This was particularly evident in the step length and joint motion range of the champion female dancer.