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1.
Br J Dermatol ; 180(5): 1107-1113, 2019 05.
Article in English | MEDLINE | ID: mdl-30311191

ABSTRACT

BACKGROUND: Epidermolysis bullosa acquisita (EBA) is a rare pemphigoid disease involving autoantibodies to type VII collagen (COL7), a major structural component of anchoring fibrils. IgE autoantibodies to type XVII collagen (BP180) have been identified in bullous pemphigoid (BP), the prototype of pemphigoid diseases. Although the pathogenic relevance of IgG anti-COL7 has been investigated, that of IgE in EBA remains unclear. OBJECTIVES: To reveal the presence and pathogenic relevance of IgE anti-COL7 in EBA. METHODS: We examined IgE antibodies in 109 patients with EBA by indirect immunofluorescence (IIF) and enzyme-linked immunosorbent assay (ELISA). RESULTS: IIF with normal human skin revealed IgE reactivity in the basement membrane zone in 29 (26·6%) cases. To verify whether the IgE antibodies were specific to COL7, we performed IIF with 21 clearly positive cases and the skin of a patient with dystrophic EBA, which does not involve COL7. All cases showed negative results, indicating that IgE antibodies were specific to COL7. In a modified IgG COL7 ELISA for IgE, 16 (14·7%) cases were positive (three and 13 cases were negative and positive on IIF, respectively). We compared anti-COL7 IgG and IgE, and found a weak but significant correlation (r = 0·459, P < 0·001). EBA is clinically divided into a mechanobullous (MB; noninflammatory) type and an inflammatory (INF) type resembling BP. Of the IIF-positive cases, 11 of 30 (37%) had INF and nine of 48 (19%) had MB. CONCLUSIONS: This study is the first to demonstrate the presence of circulating anti-COL7 IgE in patients with EBA, which may correlate with the clinical phenotype.


Subject(s)
Autoantibodies/blood , Collagen Type VII/immunology , Epidermolysis Bullosa Acquisita/immunology , Immunoglobulin E/blood , Autoantibodies/immunology , Autoantibodies/isolation & purification , Enzyme-Linked Immunosorbent Assay , Epidermolysis Bullosa Acquisita/blood , Epidermolysis Bullosa Acquisita/diagnosis , Epidermolysis Bullosa Acquisita/pathology , Fluorescent Antibody Technique, Indirect , Humans , Immunoglobulin E/immunology , Immunoglobulin E/isolation & purification , Skin/immunology , Skin/pathology
2.
Br J Dermatol ; 177(1): 152-157, 2017 Jul.
Article in English | MEDLINE | ID: mdl-27943257

ABSTRACT

BACKGROUND: Linear IgA bullous dermatosis (LABD) is a heterogeneous disease. Different diagnostic criteria have been used in different reports. OBJECTIVES: To reappraise the characteristic features of LABD with only IgA deposition at the basement membrane zone (BMZ) with direct immunofluorescence (DIF). METHODS: We retrospectively collected data from 101 patients from our archival records from 1 January 1996 to 31 December 2014 who had: (i) blisters on the skin and/or mucous membranes; (ii) subepidermal blisters in a biopsy specimen; and (iii) linear IgA deposition along the BMZ with/without linear C3 deposition at the BMZ with DIF. Most patients were referred for serological evaluation. Patients who showed concurrent linear IgG and/or IgM deposition at the BMZ under DIF were excluded. Clinical manifestations and serological findings were analysed. RESULTS: Heterogeneity of autoantigens in LABD was confirmed. Fifty-four of 101 patients (53%) had IgG antibodies detected either by indirect immunofluorescence, immunoblotting or enzyme-linked immunosorbent assays (ELISA). No statistical difference in clinical manifestations was found between patients in the IgG antibody-possessing group and patients in the other group. CONCLUSIONS: An association of IgG anti-BMZ antibodies with LABD may increase if new IgG immunoblotting or ELISA techniques become available. Consensus for diagnostic criteria for LABD is desired for prospective data storage, although it may be arbitrary.


Subject(s)
Basement Membrane/immunology , Immunoglobulin A/metabolism , Linear IgA Bullous Dermatosis/immunology , Adolescent , Adult , Age of Onset , Aged , Aged, 80 and over , Child , Child, Preschool , Enzyme-Linked Immunosorbent Assay , Female , Fluorescent Antibody Technique, Direct , Humans , Infant , Male , Middle Aged , Retrospective Studies , Young Adult
4.
Gene Ther ; 22(2): 127-37, 2015 Feb.
Article in English | MEDLINE | ID: mdl-25503692

ABSTRACT

Age-related macular degeneration (AMD) is a vision-threatening disease characterized by choroidal fibrovascular membrane (FVM) formation, choroidal neovascularization (CNV) and choroidal fibrosis. No safe and effective therapeutic method has been developed for the choroidal fibrosis, although anti-vascular endothelial growth factor therapy can partially shrink the CNV. We recently reported that periostin (POSTN), which is produced by retinal pigment epithelial cells, has an important role in the formation of preretinal FVMs, but its role in choroidal FVMs has not been determined. In this study, we used Postn knockout mice to investigate the role played by POSTN in choroidal FVM formation. In addition, we used a new class of RNA interference (RNAi) agent (NK0144) that targets POSTN and determined its effect on choroidal FVM development. Genetic ablation of Postn had an inhibitory effect not only on CNV formation but also on choroidal fibrosis in a mouse CNV model. NK0144 also had a greater inhibitory effect on both the CNV and choroidal fibrosis than control RNAi with no apparent adverse effects. These findings suggest a causal relationship between POSTN and choroidal FVM formation, and also a potential therapeutic role of intravitreal NK0144 for AMD.


Subject(s)
Cell Adhesion Molecules/genetics , Choroidal Neovascularization/therapy , Macular Degeneration/therapy , RNA Interference , Animals , Base Sequence , Cell Adhesion , Cell Adhesion Molecules/metabolism , Cell Movement , Cell Proliferation , Cells, Cultured , Choroid/blood supply , Choroid/pathology , Gene Knockdown Techniques , Genetic Therapy , Humans , Intravitreal Injections , Mice, Inbred C57BL , Mice, Knockout , Molecular Sequence Data , RNA, Small Interfering/administration & dosage , RNA, Small Interfering/genetics , Retinal Pigment Epithelium/pathology , Retinal Pigment Epithelium/physiology , Toll-Like Receptor 3/metabolism
6.
Br J Dermatol ; 171(3): 544-53, 2014 Sep.
Article in English | MEDLINE | ID: mdl-24588203

ABSTRACT

BACKGROUND: Drug-induced pemphigus (DIP) shows clinical, histopathological and immunological features of pemphigus. However, little is known about immunological profiles in DIP. OBJECTIVES: To characterize clinical and immunological profiles in patients with DIP. METHODS: We studied 17 Japanese patients with DIP who were treated at Kurume University Hospital or who consulted from other hospitals between 1997 and 2012. Complicated diseases, clinical and histopathological manifestations, responsible drugs and findings in immunofluorescence, enzyme-linked immunosorbent assays (ELISAs), immunoblotting (IB) and prognosis were analysed. RESULTS: Eight of the 17 patients with DIP showed pemphigus foliaceus-like appearance, three showed pemphigus herpetiformis-like appearance, and six showed atypical bullous lesions. Responsible drugs were thiol-containing drugs in 16 patients (bucillamine in nine cases, d-penicillamine in four cases, and cetapril, thiopronine and captopril in one patient each), and a nonthiol drug, sulfasalazine, in one patient. By ELISAs and/or IB analyses, nine patients reacted only with desmoglein 1 (Dsg1), four reacted with Dsg1 and Dsg3, and four showed no specific reactivity. By IB of normal human epidermal extracts, in addition to positive reactivity with Dsg1, four patients with no detectable malignancy showed paraneoplastic pemphigus-like reactivity with the 210-kDa envoplakin and the 190-kDa periplakin. Four cases showed anti-Dsg3 antibodies without mucosal lesions. While 11 cases recovered after discontinuation of the causative drugs, six patients had a very protracted or intractable disease course, and might develop true pemphigus. CONCLUSIONS: The present study indicated that the majority of the patients with DIP studied showed a pemphigus foliaceus-type phenotype with anti-Dsg1 autoantibodies, caused by thiol-containing drugs.


Subject(s)
Drug Eruptions/etiology , Pemphigus/chemically induced , Adult , Aged , Aged, 80 and over , Autoantibodies/immunology , Desmoglein 1/immunology , Drug Eruptions/metabolism , Female , Humans , Japan , Male , Middle Aged , Pemphigus/immunology
7.
J Phys Condens Matter ; 25(27): 275603, 2013 Jul 10.
Article in English | MEDLINE | ID: mdl-23774462

ABSTRACT

We report comprehensive measurements of the magnetic, transport, and thermal properties of the Heusler-type compound Fe2+xV1-xAl, with x values near the ferromagnetic quantum critical point, xc âˆ¼ 0.05. At T âˆ¼ 60 K, a prominent Schottky-like anomaly appeared in the specific heat; this anomaly was correlated with a smaller pseudo-gap formation in magnetic susceptibility, magnetoresistance, and thermoelectric power. Furthermore, a magnetic anomaly observed in the magnetic susceptibility and resistivity at T âˆ¼ 4 K was suppressed significantly by applying a magnetic field. A magnetically inhomogeneous phase arose below T âˆ¼ 60 K, which appeared to consist of ferromagnetic and paramagnetic clusters.


Subject(s)
Aluminum/chemistry , Magnetic Fields , Magnets/chemistry , Vanadium/chemistry , Crystallography, X-Ray , Models, Molecular , Quantum Theory , Temperature
8.
Neuropathol Appl Neurobiol ; 39(4): 406-16, 2013 Jun.
Article in English | MEDLINE | ID: mdl-22860700

ABSTRACT

AIMS: Increasing evidences suggest a similarity in the pathophysiological mechanisms of neuronal cell death in amyotrophic lateral sclerosis (ALS) and myofibre degeneration in sporadic inclusion body myositis (sIBM). The aim of this study is to elucidate the involvement of ALS-causing proteins in the pathophysiological mechanisms in sIBM. METHODS: Skeletal muscle biopsy specimens of five patients with sIBM, two with oculopharyngeal muscular dystrophy (OPMD), three with polymyositis (PM), three with dermatomyositis (DM), three with neurogenic muscular atrophy, and three healthy control subjects were examined. We analysed the expression and localization of familial ALS-causing proteins, including transactive response DNA binding protein-43 (TDP-43), fused in sarcoma/translocated in liposarcoma (FUS/TLS), Cu/Zn superoxide dismutase (SOD1) and optineurin (OPTN) by immunohistochemistry. RESULTS: TDP-43, OPTN and, to a lesser extent, FUS/TLS were more frequently accumulated in the cytoplasm in patients with sIBM and OPMD than in patients with PM, DM, neurogenic muscular atrophy, or healthy control subjects. SOD1 was accumulated in a small percentage of myofibres in patients with sIBM and OPMD, and to a very small extent in patients with PM and DM. Confocal microscopy imaging showed that TDP-43 proteins more often colocalized with OPTN than with FUS/TLS, p62 and phosphorylated Tau. CONCLUSIONS: These findings suggest that OPTN in cooperation with TDP-43 might be involved in the pathophysiological mechanisms of skeletal muscular degeneration in myopathy with rimmed vacuoles. Further investigation into these mechanisms is therefore warranted.


Subject(s)
DNA-Binding Proteins/physiology , Myositis, Inclusion Body/genetics , Myositis, Inclusion Body/pathology , TDP-43 Proteinopathies/genetics , TDP-43 Proteinopathies/pathology , Transcription Factor TFIIIA/physiology , Adult , Aged , Aged, 80 and over , Amyotrophic Lateral Sclerosis/genetics , Amyotrophic Lateral Sclerosis/pathology , Biopsy , Cell Cycle Proteins , DNA-Binding Proteins/genetics , Dermatomyositis/genetics , Dermatomyositis/pathology , Female , Humans , Immunohistochemistry , Male , Membrane Transport Proteins , Middle Aged , Muscular Atrophy/genetics , Muscular Atrophy/pathology , Muscular Dystrophy, Oculopharyngeal/genetics , Muscular Dystrophy, Oculopharyngeal/pathology , Polymyositis/genetics , Polymyositis/pathology , RNA-Binding Protein FUS/metabolism , Superoxide Dismutase/metabolism , Superoxide Dismutase-1 , Transcription Factor TFIIIA/genetics
12.
Clin Exp Dermatol ; 34(8): e573-6, 2009 Dec.
Article in English | MEDLINE | ID: mdl-19489867

ABSTRACT

We report three cases of pigmentary demarcation lines associated with pregnancy. In addition, we reviewed 19 cases including our 3 cases, which were reported in Japan. Most cases occurred during the latter period of pregnancy (after the seventh month), and the pigmentation faded spontaneously or disappeared a few months after delivery in all cases except one. Pigmentary demarcation lines are classified into five groups (types A-E). Of the 19 cases we reviewed, 2 cases showed lines of both types A and B, whereas all the other cases showed type B lines. Although there have only been 29 cases of pigmentary demarcation lines associated with pregnancy reported to date, before ours, we experienced 3 cases within 3 months, therefore it is possible that many such cases are overlooked. Pigmentary demarcation lines are mainly a cosmetic problem. Two of our three cases presented to obstetricians initially. We suggest that dermatologists should be aware that pigmentary demarcation lines may be associated with pregnancy.


Subject(s)
Hyperpigmentation/pathology , Pregnancy Complications/pathology , Adult , Asian People , Female , Humans , Pregnancy , Remission, Spontaneous , Young Adult
14.
Clin Exp Dermatol ; 33(5): 641-3, 2008 Aug.
Article in English | MEDLINE | ID: mdl-18627388

ABSTRACT

Only a few reports have been published of detailed clinical studies of pemphigus in Japan. The aim of this study was to determine the clinical characteristics of patients with pemphigus vulgaris (PV) and pemphigus foliaceous (PF), who were newly diagnosed in the dermatology department of Kurume University Hospital, Japan, over the past 11 years. The primary site of involvement was the oral mucosa in 21 patients (75%) with PV. At the initial visit, most of the patients with PV had moderate to severe disease. With regard to management, systemic corticosteroids were the mainstay of treatment for patients with PV, and plasmapheresis was the most frequently used adjuvant therapy. Dapsone was the mainstay of treatment for the patients with PF. The patients were investigated for any association with an underlying malignancy; in patients with PV, lung, stomach and uterine cancers (one patient each) were seen.


Subject(s)
Pemphigus , Adrenal Cortex Hormones/therapeutic use , Adult , Anti-Infective Agents/therapeutic use , Dapsone/therapeutic use , Diagnosis, Differential , Female , Humans , Japan/epidemiology , Male , Middle Aged , Pemphigus/drug therapy , Pemphigus/epidemiology , Pemphigus/pathology , Retrospective Studies
15.
J Biol Chem ; 276(50): 47387-93, 2001 Dec 14.
Article in English | MEDLINE | ID: mdl-11584022

ABSTRACT

An analogue of isoleucyl-adenylate (Ile-AMS) potently inhibits the isoleucyl-tRNA synthetases (IleRSs) from the three primary kingdoms, whereas the antibiotic mupirocin inhibits only the eubacterial and archaeal IleRSs, but not the eukaryotic enzymes, and therefore is clinically used against methicillin-resistant Staphylococcus aureus. We determined the crystal structures of the IleRS from the thermophilic eubacterium, Thermus thermophilus, in complexes with Ile-AMS and mupirocin at 3.0- and 2.5-A resolutions, respectively. A structural comparison of the IleRS.Ile-AMS complex with the adenylate complexes of other aminoacyl-tRNA synthetases revealed the common recognition mode of aminoacyl-adenylate by the class I aminoacyl-tRNA synthetases. The Ile-AMS and mupirocin, which have significantly different chemical structures, are recognized by many of the same amino acid residues of the IleRS, suggesting that the antibiotic inhibits the enzymatic activity by blocking the binding site of the high energy intermediate, Ile-AMP. In contrast, the two amino acid residues that concomitantly recognize Ile-AMS and mupirocin are different between the eubacterial/archaeal IleRSs and the eukaryotic IleRSs. Mutagenic analyses revealed that the replacement of the two residues significantly changed the sensitivity to mupirocin.


Subject(s)
Adenosine Monophosphate/chemistry , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/pharmacology , Isoleucine-tRNA Ligase/metabolism , Isoleucine/chemistry , Mupirocin/chemistry , Mupirocin/pharmacology , Amino Acid Sequence , Amino Acids/chemistry , Amino Acyl-tRNA Synthetases/metabolism , Binding Sites , Crystallography, X-Ray , Fatty Acids/chemistry , Inhibitory Concentration 50 , Kinetics , Models, Chemical , Models, Molecular , Molecular Sequence Data , Mutagenesis, Site-Directed , Phosphates/chemistry , Protein Conformation , Protein Structure, Secondary , Sequence Homology, Amino Acid , Staphylococcus aureus/metabolism , Thermus thermophilus
16.
Hepatology ; 33(6): 1441-50, 2001 Jun.
Article in English | MEDLINE | ID: mdl-11391533

ABSTRACT

D-Galactosamine (GalN)/lipopolysaccharide (LPS)-induced liver injury is an experimental model of fulminant hepatic failure in which tumor necrosis factor alpha (TNF-alpha) plays a pivotal role. We examined the effects of etoposide on GalN/LPS-induced fulminant hepatic failure. Mice were given an intraperitoneal dose of GalN (800 microg/g body weight)/LPS (100 ng/g body weight) with and without intraperitoneal etoposide (10 microg/g body weight) treatment. Liver injury was assessed biochemically and histologically. TNF-alpha levels in the serum, and apoptosis of hepatocytes and CPP32/caspase-3 in the liver, were determined. GalN/LPS treatment caused lethal liver injury in 87% of animals (13 of 15). The effect was associated with significant increases in TNF-alpha and alanine transaminase (ALT) levels in serum, the number of apoptotic hepatocytes, CPP32/caspase-3 activity, and TNF receptor 1 (TNFR1) mRNA expression in the liver. Etoposide (10 microg/g body weight) was given 3 times (at 50, 26, and 4 hours before GalN/LPS administration). Treatment of GalN/LPS-treated mice with etoposide reduced apoptosis of hepatocytes, resulting in reduction of lethality (13% [2 of 15]), while another topoisomerase II inhibitor, IRCF-193, showed no significant effect. The antilethal effect of etoposide was also confirmed in GalN/TNF-alpha-induced fulminant hepatic failure. Etoposide treatment reduced CPP32/caspase-3 activity in the liver, although it did not alter the serum TNF-alpha levels or hepatic TNFR1 mRNA expressions. In addition, etoposide treatment enhanced the mRNA and protein expression of Bcl-xL, an antiapoptotic molecule in the liver. The present findings suggest that etoposide prevents endotoxin-induced lethal liver injury by up-regulation of Bcl-xL, and that etoposide could be useful for the treatment of TNF-alpha-mediated liver diseases.


Subject(s)
Antineoplastic Agents, Phytogenic/pharmacology , Apoptosis/drug effects , Etoposide/pharmacology , Liver Failure/mortality , Liver Failure/physiopathology , Liver/drug effects , Liver/physiopathology , Animals , Antigens, CD/genetics , Caspase 3 , Caspase Inhibitors , Cytochrome c Group/antagonists & inhibitors , Cytoplasm/metabolism , Galactosamine , Hepatocytes/drug effects , Hepatocytes/metabolism , Lipopolysaccharides , Liver/metabolism , Liver/pathology , Liver Failure/chemically induced , Liver Failure/prevention & control , Male , Mice , Mice, Inbred BALB C , Proto-Oncogene Proteins c-bcl-2/genetics , RNA, Messenger/metabolism , Receptors, Tumor Necrosis Factor/genetics , Receptors, Tumor Necrosis Factor, Type I , Tumor Necrosis Factor-alpha/metabolism , bcl-X Protein
17.
Biochem Biophys Res Commun ; 280(1): 368-73, 2001 Jan 12.
Article in English | MEDLINE | ID: mdl-11162525

ABSTRACT

Several growth factors play an important role in liver regeneration. Once hepatic injury occurs, liver regeneration is stimulated by hepatocyte growth factor (HGF), transforming growth factor (TGF)-alpha, and heparin-binding epidermal growth factor-like growth factor (HB-EGF), whereas TGF-beta1 terminates liver regeneration. In this study, we analyzed the effect of a combination of HGF and epidermal growth factor (EGF) on mitogen-activated protein kinase (MAPK) activity and G1 cyclin expression in primary cultured rat hepatocytes. Treatment with a combination of HGF and EGF, in comparison with that of either HGF or EGF, induced tyrosine phosphorylation of both c-Met and EGF receptor (EGFR) independently and additively stimulated MAPK activity and cyclin D1 expression, resulting in additive stimulation of DNA synthesis. On the other hand, although TGF-beta1 treatment did not affect tyrosine phosphorylation of c-Met and EGFR, MAPK activity, and cyclin D1 expression, which were stimulated by HGF and EGF, DNA synthesis was completely inhibited through a marked decrease in cyclin E expression. These results indicate that potent mitogens, such as HGF, TGF-alpha, and HB-EGF, could induce the additive enhancement of liver regeneration cooperatively through an increase in Ras/MAPK activity followed by cyclin D1 expression, and that TGF-beta1 suppresses the growth factor-induced signals between cyclin D1 and cyclin E, resulting in the inhibition of DNA synthesis.


Subject(s)
Cyclin D1/physiology , DNA/biosynthesis , Growth Substances/pharmacology , Hepatocytes/physiology , MAP Kinase Signaling System/physiology , Mitogen-Activated Protein Kinases/metabolism , Animals , Cells, Cultured , Cyclin D1/biosynthesis , Epidermal Growth Factor/pharmacology , G1 Phase , Hepatocyte Growth Factor/pharmacology , Hepatocytes/cytology , Hepatocytes/drug effects , Kinetics , Liver/cytology , Male , Rats , Rats, Wistar , Transforming Growth Factor beta/pharmacology
19.
J Gastroenterol ; 35(8): 641-5, 2000.
Article in English | MEDLINE | ID: mdl-10955605

ABSTRACT

Inflammatory pseudotumor of the liver is a rare benign lesion, but exploratory laparotomy and a hepatectomy are often performed unnecessarily after various misdiagnoses, including liver abscess, hepatocellular carcinoma, metastatic liver tumor, and cholangiocarcinoma. We present a case of hepatic inflammatory pseudotumor in a 17-year-old man in whom diagnosis was confirmed by liver needle biopsy under ultrasonographic tomography (UST) guidance. He had complained of fever and right hypochondralgia 2 months after being operated for appendicitis. He was admitted to our hospital because of the persistence of these symptoms and the presence of a hepatic mass lesion detected by UST. He had hepatomegaly, with tenderness; leukocytosis and elevated erythrocyte sedimentation rate and C-reactive protein level were noted. UST showed a hypoechoic mass in the liver and pre-contrast computerized tomography (CT) revealed a low-density area with an ill defined margin, which was barely enhanced by the contrast medium. On the basis of the patient's clinical symptoms and the laboratory data and imaging studies, the presence of a liver abscess was suspected and antibiotics were administered. One month after the initiation of the antibiotic therapy, UST demonstrated that the portal vein had dilated serpiginously and penetrated into the mass. As the heterogeneous appearance displayed by post-enhanced CT indicated the need for a differential diagnosis of the hepatic mass lesion to rule out hepatocellular carcinoma, percutaneous needle biopsy was performed, under UST guidance. Histopathological examination demonstrated marked infiltration of plasma cells and fibrosis, findings which were consistent with those of hepatic inflammatory pseudotumor. There was a spontaneous reduction of the hepatic pseudotumor without continuous antibiotics and this reduction was documented on follow-up UST and CT.


Subject(s)
Granuloma, Plasma Cell/pathology , Liver Diseases/pathology , Adolescent , Biopsy, Needle/methods , Diagnosis, Differential , Granuloma, Plasma Cell/diagnosis , Granuloma, Plasma Cell/diagnostic imaging , Humans , Liver Abscess/diagnosis , Liver Diseases/diagnosis , Liver Diseases/diagnostic imaging , Liver Neoplasms/diagnosis , Male , Ultrasonography
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