ABSTRACT
In a diffusion chamber (DC) system, cells are cultured in vivo - hence making it possible to minimize infection and foreign material contamination. In view of this merit, we devised a technique to combine a DC system and a scaffold to the end of incubating sufficient host cells for grafting. In the present study, PLGA sponge and rat bone marrow cells were encapsulated inside a DC and then placed inside the abdominal cavities of rats. DCs were removed at two or four weeks after grafting. At four weeks after grafting, fibrous and calcified tissue matching the shape of the PLGA sponge was formed. These results suggested that the PLGA sponge was an effective scaffolding material in inducing three-dimensional tissue formation and that combination with a DC system resulted in a cell mass matching the scaffold shape. In addition, the cells were cultured in vivo - which meant that DC culturing did not require special incubation facilities or technologies after grafting.
Subject(s)
Cell Culture Techniques , Diffusion Chambers, Culture , Tissue Engineering/methods , Tissue Scaffolds , Animals , Biocompatible Materials , Bone Marrow Cells , Calcium/analysis , Cells, Cultured , Lactic Acid , Male , Polyglycolic Acid , Polylactic Acid-Polyglycolic Acid Copolymer , Rats , Rats, Sprague-DawleyABSTRACT
We sought to investigate whether the photocatalytic function of rutile-type titanium dioxide is applicable for orthodontic brackets. To this end, TiO(2 )specimens were compressed and sintered. Then, each specimen was analyzed by X-ray diffraction to confirm that no compositional changes had taken place. Next, the surface of each specimen was observed with a colorimeter, scanning electron microscope (SEM), and atomic force microscope (AFM). Fundamental material properties after calcination were measured. The photocatalytic function was evaluated using the decolorization method with toluidine blue. The following were observed with the increase of calcinations temperature: growth of crystal particles, decrease in surface roughness and surface brightness, but increase in shrinkage percentage and compressive strength. The aesthetic and mechanical properties of TiO(2 )material were adequate when compared with other bracket materials. In addition, it had a satisfactory photocatalytic function after high-temperature calcination. Based on the favorable results obtained, rutile-type titanium dioxide seemed to be applicable for the fabrication of self-cleaning orthodontic brackets.
Subject(s)
Dental Alloys/chemistry , Orthodontic Appliance Design , Orthodontic Brackets , Titanium/chemistry , Compressive Strength , Crystallization , Decontamination/methods , Dental Stress Analysis , Hot Temperature , Photochemical Processes , Stress, Mechanical , Surface PropertiesABSTRACT
Successful tissue engineering relies on a combination of cells, cytokines, and appropriate scaffolds. Here, we tried to induce the formation of cartilage in vitro using immature muscular tissue, crude bone morphogenetic protein (BMP) as a source of the cytokine, and biodegradable membranes (BioMend; BM and GC-membrane; GC-M) or a nonbiodegradable membrane (GORE-TEX; GT) as scaffolds. Crude BMP was extracted from bovine cortical bones, dried, and dissolved in 1M urea before it was added to immature muscular tissue from the forelimbs of fetal Sprague Dawley rats at 20 days of pregnancy. The tissue was then cultured for 2 weeks in a carbon dioxide incubator. Complete cartilage was observed only when GT was used as a scaffold. In addition, cartilage-like tissue formation was observed when BM was used, and partial cartilage formation was observed for GC-M. Therefore, these results show that immature muscular tissue differentiated into cartilage and GORE-TEX is the most effective material for use as a scaffold in this model of tissue engineering.
