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1.
Microsc Res Tech ; 82(10): 1706-1719, 2019 Oct.
Article in English | MEDLINE | ID: mdl-31294498

ABSTRACT

INTRODUCTION: Procedures for measuring and counting tracks are time-consuming and involve practical problems. The precision of automatic counting methods is not satisfactory yet; the major challenges are distinguishing tracks and material defects, identifying small tracks and defects of similar size, and detecting overlapping tracks. MATERIALS AND METHODS: Here, we address the overlapping tracks issue using the algorithm Watershed Using Successive Erosions as Markers (WUSEM), which combines the watershed transform, morphological erosions and labeling to separate regions in photomicrographs. We tested this method in two data sets of diallyl phthalate (DAP) photomicrographs and compared the results when counting manually and using the classic watershed and H-watershed transforms. RESULTS: The mean automatic/manual efficiency counting ratio when using WUSEM in the test data sets is 0.97 ± 0.11. CONCLUSION: WUSEM shows reliable results when used in photomicrographs presenting almost isotropic objects. Also, diameter and eccentricity criteria may be used to increase the reliability of this method.

2.
Microsc Res Tech ; 81(1): 22-32, 2018 Jan.
Article in English | MEDLINE | ID: mdl-29052281

ABSTRACT

Image segmentation, the process of separating the elements within a picture, is frequently used for obtaining information from photomicrographs. Segmentation methods should be used with reservations, since incorrect results can mislead when interpreting regions of interest (ROI). This decreases the success rate of extra procedures. Multi-Level Starlet Segmentation (MLSS) and Multi-Level Starlet Optimal Segmentation (MLSOS) were developed to be an alternative for general segmentation tools. These methods gave rise to Jansen-MIDAS, an open-source software. A scientist can use it to obtain several segmentations of hers/his photomicrographs. It is a reliable alternative to process different types of photomicrographs: previous versions of Jansen-MIDAS were used to segment ROI in photomicrographs of two different materials, with an accuracy superior to 89%.

3.
Appl Spectrosc ; 68(5): 549-56, 2014.
Article in English | MEDLINE | ID: mdl-25014598

ABSTRACT

Spectroscopic and morphological studies, designed to improve our understanding of the physicochemical phenomena that occur during zircon crystallization, are presented. The zircon fission track method (ZFTM) is used routinely in various laboratories around the world; however, there are some methodological difficulties needing attention. Depending on the surface fission track density observed under an optical microscope, the zircon grain surfaces are classified as homogeneous, heterogeneous, hybrid, or anomalous. In this study, zircon grain surfaces are characterized using complementary techniques such as optical microscopy (OM), micro-Raman spectroscopy, and scanning electron microscopy (SEM), both before and after chemical etching. Our results suggest that anomalous grains have subfamilies and that etching anisotropy related to heterogeneous grains is due to different crystallographic faces within the same polished surface that cannot be observed under an optical microscope. The improved methodology was used to determine the zircon fission track ages of samples collected from the Bauru Group located in the north of Paraná Basin, Brazil. A total of 514 zircon grains were analyzed, consisting of 10% homogeneous, about 10% heterogeneous, about 20% hybrid, and 60% anomalous grains. These results show that the age distributions obtained for homogeneous, heterogeneous, and hybrid grains are both statistically and geologically compatible.

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