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J Plant Physiol ; 170(18): 1553-60, 2013 Dec 15.
Article in English | MEDLINE | ID: mdl-23850031

ABSTRACT

Common reed (Phragmites australis) is a phytoremediator tolerant to heavy metals. In this study, we found that 70% of the cadmium (Cd) found in the stem of common reed exists in a soluble form, with more than half of the soluble Cd in the 10- to 50-kDa fraction. Based on an enzyme degradation assay, the major component of the Cd-associated molecule is assumed to be an amylopectin-like α-glucan. This molecule may associate with Cd via the carboxyl group, rather than the thiol group. The conditions required for the disengagement of Cd from the 10- to 50-kDa fraction indicated that disulfide bonds and other intramolecular interactions may contribute to maintaining the proper conformation of the molecule and to stabilizing its association with Cd. Accumulation of the Cd-associated molecule was induced by Cd stress, and the molecule was found to be also associated with Cu and Fe. Thus, we have identified a novel mechanism of Cd-pooling, namely, the association of Cd with an α-glucan-like molecule in reed stem.


Subject(s)
Cadmium/isolation & purification , Cadmium/toxicity , Glucans/metabolism , Plant Stems/metabolism , Poaceae/metabolism , Chemical Fractionation , Disulfides/metabolism , Hydrogen-Ion Concentration/drug effects , Mercaptoethanol/pharmacology , Molecular Weight , Phosphates/metabolism , Plant Exudates/metabolism , Plant Stems/cytology , Plant Stems/drug effects , Poaceae/drug effects , Solubility , Sulfhydryl Compounds/metabolism , Ultrafiltration
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