ABSTRACT
Microfluidics is a rapidly growing field in which small volumes of liquid are moved through channels in a large variety of applications. Fabricating such channels can be expensive. Here, we describe an inexpensive method for making 3D channels in fluidic chips by using a sacrificial template made of coated metal wire or metal tubes. A 3D template is embedded in polymer or glass and then dissolved, leaving channels in the chip, without the need for expensive instruments. By changing the mold, chips of various shapes can be made.
Subject(s)
Dimethylpolysiloxanes/chemistry , Glass/chemistry , Lab-On-A-Chip Devices , Metals/chemistry , Microfluidics/instrumentation , Aluminum Oxide/chemistry , Copper/chemistry , Cost-Benefit Analysis , Equipment Design , Lab-On-A-Chip Devices/economics , Microfluidics/economics , Polyethylene Glycols/chemistry , Solubility , Steel/chemistry , Surface PropertiesABSTRACT
Adenine, adenosine-5'-monophosphate and adenosine-5'-triphosphate were separated by fluidic chip with PAA membranes using an aqueous mobile phase.
Subject(s)
Adenine/isolation & purification , Adenosine Monophosphate/isolation & purification , Adenosine Triphosphate/isolation & purification , Aluminum Oxide/chemistry , Chromatography, Liquid/instrumentation , Microfluidic Analytical Techniques/instrumentation , Equipment Design , Hydrogen-Ion Concentration , Nanostructures , Reproducibility of ResultsABSTRACT
The possibility of using porous anodic alumina membranes as a column for normal-phase high performance liquid chromatography was evaluated using phenol and toluene with mobile phases having different solvent compositions.
Subject(s)
Aluminum Oxide/chemistry , Chromatography, High Pressure Liquid/methods , Nanostructures/chemistry , Phenol/chemistry , Toluene/chemistryABSTRACT
The permeation fluxes of phenol, benzene sulfonate (BS) and benzene disulfonate (BDS) through a porous anodic alumina membrane with the perpendicularly oriented silica-surfactant nanochannel assembly membrane (NAM) were measured in water-ethanol mixture media. The permeation flux depended on solute charges and on solvent composition. As the ethanol ratio increased, the fluxes of BS and BDS increased and the flux of phenol decreased. The results of extraction/elution experiments also depended on the solute charges and the solvent composition. Chromatographic experiments in n-hexane showed that dipole and hydrophobic interactions affect the retention of solutes. Permeation of the solute across the NAM in water-ethanol mixture is likely to be determined by various factors such as dipole interaction, hydrophobic interaction, solvation, and anion-exchange efficiencies.
