Primary and secondary sludge treatment using ionizing radiation technology in Alexandria, Egypt.

Improving the treatment efficiency of sludge in Alexandria, Egypt, was studied to improve the primary and secondary sludge treatment efficiency, different doses ranging from 0.25 to 6 kGy of ionizing radiation were proposed and evaluated. The scope of This study is to assess the radiation-based treatment efficiency from physical, chemical, and biological perspectives and to compare between the conventional treatment method and the radiation-based treatment technology. To evaluate the performance of each treatment system, pH, oil and greases concentrations, total solid concentrations, BOD concentrations, COD concentrations, parasites, and microorganisms were assessed in the primary and secondary samples at different radiation doses (from 0.25 to 6 KGy), and in the conventionally treated samples. Irradiation by gamma radiation with a dose ranging from 0.25 to 6 kGy was efficient in reducing some of the physical contaminants. Oil & greases, Total Solids, BOD, COD concentrations were reduced significantly (p < 0.001) in a dose-dependent manner. Either primary or secondary sludge samples, total solid reduced significantly to about one-third of control concentration at six kGy. Six kGy able to reduce the BOD and COD concentrations in the primary sludge samples to that of the treated (after sludge dewatering) samples or less respectively and saved the secondary treatment stage. In primary and secondary sludge samples culture, E. coli, Staphylococcus aureus, and Vibrio spp were isolated as heavy growth on different culture media in the samples before radiation. After exposure to increasing doses of radiation, the number of isolated organisms decreased, however, the growth of Proteus, Acinetobacter, and vipro organisms was detected but in small numbers. No growth of any organism was noted at 5 kGy. On the other hand, in the secondary sludge samples, Proteus was isolated as heavy growth before radiation and After exposure to increasing doses of radiation, Moraxella spp. organisms were detected but in small numbers. By increasing the radiation doses, the free-living ciliates were decreased in the primary and secondary sludge samples. The free-living ciliates disappeared completely at 3 kGy. In the primary and secondary sludge samples, free-living ciliates reduction efficiency at 0.25 kGy was equivalent to the conventional treatment methods. So, we can conclude that radiation technology using Gamma rays at a dose higher than 5 kGy with a dose rate of 1.095 kGy/h is an effective technology for domestic and industrial waste sludge treatment from the environmental perspective and an experimental pilot plant study is required to optimize the cost of wastewater treatment through the use of irradiation technology.

Radiosensitizing and Phototherapeutic Effects of AuNPs are Mediated by Differential Noxa and Bim Gene Expression in MCF-7 Breast Cancer Cell Line.

To compare the apoptotic efficiency of AuNPs, ionizing and non-ionizing radiotherapy, phototherapy, and AuNPs-ionizing-radiotherapy), MCF-7 cells were used as a model for luminal B subtypes of breast carcinoma. A mixture of AuNPs [66% of Au-nanospheres (AuNSs) and 34% of Au-nanorods (AuNRs)] was synthesized and characterized by optical spectroscopy, zeta potential, and transmission electron microscopy (TEM). MCF-7 were divided into six groups (triplicates); after each treatment, cell viability was tested by MTT assay and relative gene expression levels of Bim and Noxa proapoptotic markers were assayed by qRT-PCR. A dose-dependent significant reduction in cell viability of MCF-7 was detected by all examined treatment protocols. Lower viability detected at extended exposure (48 hours) to AuNPs ( [Formula: see text]/ml) was mediated by the upregulation of Noxa gene expression. AuNS and AuNR in vitro PTTs were mediated by differential expression of Bim and Noxa while AuNPs mixture had a combined effect on both Bim and Noxa. Cellular recovery was observed two days-post x-rays irradiation at does < 3 Gy. AuNPs showed dose enhancement factor (DEF) > 12 indicating a high radiosensitizing effect that was partially mediated by Noxa. In conclusion, AuNPs combined therapies exert better anti-proliferative effects via differential regulation of Noxa and Bim gene expressions.

Exposure to Radiation During Work Shifts and Working at Night Act as Occupational Stressors Alter Redox and Inflammatory Markers.

BACKGROUND: Studies of breast cancer etiology suggest evidence that night shift working and occupational exposure to ionizing radiation (IR) are defined risk factors for breast cancer development. There are few studies to clarify neuroendocrine and inflammatory status and the possible consequences particularly in occupational exposure. AIM OF THE STUDY: Our aim was to associate the redox and inflammatory biomarkers with either nightshift working or occupational radiation exposure, and to compare their levels between the two groups at Alexandria University Hospitals, Alexandria, Egypt. METHODS: We included 150 female nurses at Alexandria University Hospitals: 50 nightshift workers, 50 radiation workers, and 50 dayshift workers as a control group (neither work nightly nor radiation workers). In morning serum sample (7 am), we measured the concentrations of serum melatonin, Cortisol, tumor necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ) by ELISA; malondialdehyde (MDA) and total antioxidant capacity (TAC) levels colorimetrically, and C-reactive protein (C-RP) levels by turbidimetric method. RESULTS: Nightshift workers had significantly lower levels of melatonin and TAC, and higher levels of serum inflammatory markers and cortisol, than day shift control group of workers. Workers occupationally exposed to IR had significantly higher levels of serum melatonin, MDA and inflammatory markers, lower levels of serum cortisol, and lower TAC than day shift workers. CONCLUSION: Occupational exposure to IR and working nightly alter circulating redox and inflammatory biomarkers.