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1.
J Microbiol Biotechnol ; 32(1): 72-80, 2022 Jan 28.
Article in English | MEDLINE | ID: mdl-34750286

ABSTRACT

In this study, the survival capacity (acid and bile salt tolerance, and adhesion to gut epithelial cells) and probiotic properties (enzyme activity-inhibition and anti-inflammatory activities, inhibition of adipogenesis, and stress hormone level reduction) of Lactiplantibacillus plantarum LRCC5314, isolated from kimchi (Korean traditional fermented cabbage), were investigated. LRCC5314 exhibited very stable survival at ph 2.0 and in 0.2% bile acid with 89.9% adhesion to Caco-2 intestinal epithelial cells after treatment for 2 h. LRCC5314 also inhibited the activities of α-amylase and α-glucosidase, which are involved in elevating postprandial blood glucose levels, by approximately 72.9% and 51.2%, respectively. Treatment of lipopolysaccharide (LPS)-stimulated RAW 264.7 cells with the LRCC5314 lysate decreased the levels of the inflammatory factors nitric oxide, tumor necrosis factor (TNF-α), interleukin (IL)-1ß, and interferon-γ by 88.5%, 49.3%, 97.2%, and 99.8%, respectively, relative to those of the cells treated with LPS alone. LRCC5314 also inhibited adipogenesis in differentiating preadipocytes (3T3-L1 cells), showing a 14.7% decrease in lipid droplet levels and a 74.0% decrease in triglyceride levels, as well as distinct reductions in the mRNA expression levels of adiponectin, FAS, PPAR/γ, C/EBPα, TNF-α, and IL-6. Moreover, LRCC5314 reduced the level of cortisol, a hormone with important effect on stress, by approximately 35.6% in H295R cells. L. plantarum LRCC5314 is identified as a new probiotic with excellent in vitro multifunctional properties. Subsequent in vivo studies may further demonstrate its potential as a functional food or pharmabiotic.


Subject(s)
Fermented Foods/microbiology , Lactobacillaceae/isolation & purification , Lactobacillaceae/physiology , Probiotics/pharmacology , 3T3-L1 Cells , Adipogenesis/drug effects , Animals , Anti-Inflammatory Agents/pharmacology , Bile Acids and Salts , Caco-2 Cells , Cytokines/metabolism , Drug Tolerance , Fermentation , Humans , Interferon-gamma/metabolism , Interleukin-1beta/metabolism , Lipopolysaccharides/adverse effects , Mice , Nitric Oxide , RAW 264.7 Cells , Tumor Necrosis Factor-alpha
2.
J Microbiol Biotechnol ; 32(3): 324-332, 2022 Mar 28.
Article in English | MEDLINE | ID: mdl-34949748

ABSTRACT

The incidence of stress-related type 2 diabetes (stress-T2D), which is aggravated by physiological stress, is increasing annually. The effects of Lactobacillus, a key component of probiotics, have been widely studied in diabetes; however, studies on the effects of postbiotics are still limited. Here, we aimed to examine the mechanism through which heat-killed Lactiplantibacillus plantarum LRCC5314 (HK-LRCC5314) alleviates stress-T2D in a cold-induced stress-T2D C57BL/6 mouse model. HK-LRCC5314 markedly decreased body weight gain, adipose tissue (neck, subcutaneous, and epididymal) weight, and fasting glucose levels. In the adipose tissue, mRNA expression levels of stress-T2D associated factors (NPY, Y2R, GLUT4, adiponectin, and leptin) and pro-inflammatory factors (TNF-α, IL-6, and CCL-2) were also altered. Furthermore, HK-LRCC5314 increased the abundance of Barnesiella, Alistipes, and butyrate-producing bacteria, including Akkermansia, in feces and decreased the abundance of Ruminococcus, Dorea, and Clostridium. Thus, these findings suggest that HK-LRCC5314 exerts protective effects against stress-T2D via gut microbiome modulation, suggesting its potential as a supplement for managing stress-T2D.


Subject(s)
Diabetes Mellitus, Type 2 , Gastrointestinal Microbiome , Lactobacillus plantarum , Probiotics , Animals , Diabetes Mellitus, Type 2/metabolism , Diabetes Mellitus, Type 2/microbiology , Hot Temperature , Lactobacillaceae , Lactobacillus plantarum/metabolism , Mice , Mice, Inbred C57BL , Probiotics/pharmacology
3.
Nutrients ; 13(8)2021 Jul 31.
Article in English | MEDLINE | ID: mdl-34444827

ABSTRACT

Cutaneous wound healing comprises a complex systemic network. Probiotics, naturally extracted substances, medicine, and chemical compounds have been used for wound healing, but the application of postbiotics as therapeutic agents has yet to be explored. Our study shows potential beneficial effects of heat-killed Lactococcus chungangensis CAU 1447 on type 1 diabetic mice. The postbiotic strain significantly decreased the skin wound size. The activity of myeloperoxidase secreted from neutrophils also decreased. The molecular mechanism of wound healing was adjusted by important mediators, growth factors, chemokines, and cytokines. These elements regulated the anti-inflammatory activity and accelerated wound healing. To determine the role of the postbiotic in wound repair, we showed a similar taxonomic pattern as compared to the diabetic mice using skin microbiome analysis. These findings demonstrated that heat-killed Lactococcus chungangensis CAU 1447 had beneficial effects on wound healing and can be utilized as postbiotic therapeutic agents.


Subject(s)
Diabetes Mellitus, Experimental , Diabetes Mellitus, Type 1 , Lactococcus/chemistry , Probiotics/therapeutic use , Skin/drug effects , Wound Healing , Animals , Chemokines/metabolism , Cytokines/metabolism , Hot Temperature , Humans , Intercellular Signaling Peptides and Proteins/metabolism , Lactococcus/genetics , Male , Mice , Mice, Inbred C57BL , Peroxidase/analysis , Skin/pathology , Streptozocin/adverse effects
4.
J Dairy Sci ; 102(12): 10737-10747, 2019 Dec.
Article in English | MEDLINE | ID: mdl-31521345

ABSTRACT

Alcoholic liver disease (ALD) is correlated with alcohol consumption, and ALD progression depends on various factors. Some lactic acid bacteria (LAB) are beneficial for mitigating ALD. However, the valuable effects of LAB-derived dairy products remain unclear. Here, we evaluated the effects of Lactococcus chungangensis CAU 1447 dry cells (CAU 1447) and cream cheese derived from CAU 1447 on ALD progression following long-term alcohol consumption in rats. Oral administration of CAU 1447 and CAU 1447 cream cheese significantly reduced alkaline phosphatase, aspartate aminotransferase, alanine aminotransferase, and triglyceride levels. We found that CAU 1447 and CAU 1447 cream cheese downregulated mRNA encoding various cytokines and antioxidative factors in the liver. Oral CAU 1447 cream cheese administration increased short-chain fatty acid, butyrate, and acetate levels in feces. Thus, administration of CAU 1447 and CAU 1447 cream cheese induced hepatoprotective effects, indicating potential applications as a supplement for ALD mitigation.


Subject(s)
Lactococcus , Liver Diseases, Alcoholic/therapy , Probiotics/therapeutic use , Animals , Biological Factors/therapeutic use , Cheese/microbiology , Dietary Supplements , Feces/chemistry , Fermentation , Male , Protective Agents/therapeutic use , Rats , Rats, Sprague-Dawley
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