ABSTRACT
Lack of clear clinical and laboratory picture of subjective evaluation of bowel viability, the progression of bowel necrosis in the postoperative period with acute mesenteric ischemia (ÐÐI) contributes to the high mortality rate. Earlier experiments we proved that gut necrosis leads to changes in the subpopulation structure of blood lymphocytes. This prompted us to determine the clinical significance of the subpopulation structure of venous blood lymphocytes in patients with acute mesenteric ischemia. The paper is based on a retrospective analysis of the results of a controlled clinical and immunological examination of 18 patients aged 62 to 78 years (control group and a group of ÐÐI). Evaluation lymphocyte subpopulation structure was performed by the standard method of direct immunofluorescence staining of whole blood. The obtained data were processed with nonparametric statistical methods. Study of lymphocyte subpopulation structure in patients with ÐÐI patients showed a decrease in the absolute and relative number of CD8, CD4, B, NK cells on the indicators in the control group. Ischemia and necrosis of the intestinal mucosa accompanied by a massive translocation of intestinal microflora through the impaired intestinal barrier along with the migration of lymphocytes into the lesion and death, which is manifested in a decrease in the number of lymphocytes of the peripheral blood. Comprehensive assessment of venous blood lymphocyte subpopulation structure can be used as an additional diagnostic criterion necrotic step ÐÐI, serve as criteria for selection of patients for immunotherapy.
Subject(s)
Intestines/pathology , Mesenteric Ischemia/diagnosis , Aged , Cell Movement , Humans , Lymphocyte Count , Lymphocytes/cytology , Middle Aged , Retrospective StudiesABSTRACT
The serum levels of some indices of the LPO antioxidant system (dienic conjugates, antioxidative activity), cytokines (IL-1beta, TNF-alpha, IL-4) and the blood antiprotease system protein alpha2-macroglobulin were studied in 97 patients with closed long bone fractures. Based on the finding, a procedure was devised to evaluate the course of an inflammatory process in the early postoperative period, which predicted the development of pyoinflammatory complications at a stage of preclinical manifestations.
Subject(s)
Antioxidants/metabolism , Cytokines/blood , Fractures, Bone/blood , Inflammation/blood , Inflammation/diagnosis , Adult , Female , Fractures, Bone/complications , Fractures, Bone/diagnosis , Fractures, Bone/surgery , Humans , Inflammation/etiology , Male , Middle Aged , Postoperative Complications/blood , Postoperative Complications/diagnosis , Postoperative PeriodABSTRACT
Interleukine (IL-1alpha, IL-4) blood levels and lipid peroxidation--antioxidant agents were assessed in 98 patients with fractures of long tubular bones. Using the data, an algorithm was worked out, which permits prediction of septic complications with a high level of accuracy.
Subject(s)
Fractures, Bone/surgery , Fractures, Closed/surgery , Interleukin-1alpha/blood , Interleukin-4/blood , Surgical Wound Infection/diagnosis , Fractures, Bone/blood , Fractures, Bone/classification , Fractures, Closed/blood , Fractures, Closed/classification , Humans , Lipid Peroxidation , Prognosis , Suppuration/blood , Suppuration/diagnosis , Surgical Wound Infection/bloodABSTRACT
An investigation of the content of interleukins (IL-1alpha, IL-4) in blood serum, indices of the system of "LP-antioxidants" (DC, AOA) was performed in 92 patients with open fractures of long tubular bones. A method of early diagnosis of purulent complications in the postoperative period was worked out which allows timely necessary correction to be introduced in the plans of treatment.
Subject(s)
Fracture Fixation/methods , Fractures, Bone/surgery , Fractures, Open/surgery , Surgical Wound Infection/diagnosis , Femoral Fractures/surgery , Follow-Up Studies , Humans , Humeral Fractures/surgery , Oxidative Stress , Prognosis , Radius Fractures/surgery , Suppuration , Surgical Wound Infection/etiology , Surgical Wound Infection/metabolism , Tibial Fractures/surgerySubject(s)
Cytokines/blood , Fractures, Open/diagnosis , Surgical Wound Infection/diagnosis , Abscess/complications , Abscess/diagnosis , Abscess/immunology , Adult , Algorithms , Cellulitis/complications , Cellulitis/diagnosis , Cellulitis/immunology , Extremities/injuries , Female , Fractures, Open/complications , Fractures, Open/immunology , Humans , Male , Middle Aged , Osteomyelitis/complications , Osteomyelitis/diagnosis , Osteomyelitis/immunology , Periostitis/complications , Periostitis/diagnosis , Periostitis/immunology , Predictive Value of Tests , Prognosis , Surgical Wound Infection/immunologyABSTRACT
Under study was the influence of cytomedins on the system of immunity in patients with developed surgical infection in a traumatology clinic. The investigation of immune reactions under conditions of purulent infection of different etiology has shown the necessity to include the up-to-date and purposeful immunomodulating therapy (cytomedins) in the complex of treatment which allows arrest of the inflammatory process in the wound in earlier terms.
Subject(s)
Peptides/immunology , Peptides/therapeutic use , Surgical Wound Infection/drug therapy , Wound Healing/drug effects , Adolescent , Adult , Anti-Ulcer Agents/therapeutic use , Combined Modality Therapy , Female , Humans , Male , Surgical Wound Infection/immunology , Surgical Wound Infection/therapy , Time Factors , Treatment Outcome , Wound Healing/immunologyABSTRACT
Effect of tissue ischemia on immunity was studied in patients with developing surgical infection after injuries. Tissue hypoxia is one of the factors promoting the formation of immunity failure. Study of the course of immune reactions under conditions of hypoxia in suppurative infection of different etiology opens new diagnostic criteria and demonstrates the necessity of a differentiated approach to immunomodulating therapy of patients with local and total surgical infections of different etiology.
