[NEUROPROTECTIVE EFFECT OF AMINOGUANIDINE IN EXPERIMENTAL OBSTETRIC ANTIPHOSPHOLIPID SYNDROME].

The aim of the research was to investigate the effect of aminoguanidine on the content of autoantibodies in the serum, nitric oxide synthesis (NO), glial fibrillary acidic protein (GFAP) content and indicators of free radical oxidation in the cerebral hemispheres of BALB/c mice with antiphospholipid syndrome (APS) on the 18th day of pregnancy. An increase in the content of autoantibodies to brain proteins (120 kDa, 150 kDa, and >170 kDa) was detected in the serum of BALB/c mice with APS on the 18th day of pregnancy. An increase in the content of GFAP (total), GFAP (49-37 kDa), NO2¯, NO3¯ and prooxidant-antioxidant system imbalance in the cerebral hemispheres of pregnant mice with APS was established. With administration of aminoguanidine into the pregnant mice with APS, a decrease in the content of autoantibodies to brain proteins (120 kDa and 150 kDa) in serum was proved. With the introduction of aminoguanidine, a selective inhibitor of inducible NO synthase on the 18th day of pregnancy the increase in GFAP (49-37 kDa) in the cerebral hemispheres of APS mice was established, and the GFAP (total), NO2¯ and NO3¯ content did not change significantly, relative to the indicators of pregnant animals with APS. With introduction of aminoguanidine in cases of APS on the 18th day of pregnancy lesser manifestations of oxidative stress in the cerebral hemispheres, a decrease in the activity of lipid peroxidation processes, an increase in the activity and content of the antioxidant system components was evidenced. Thus, aminoguanidine has a neuroprotective effect in obstetric antiphospholipid syndrome in the BALB/c mice.

[The role of blood rheology and micro hemocirculation in development of generalized hypoxia in crush syndrome].

Crush syndrome (CS) is a type of traumatic pathology accompanied by intoxication of organism with a heavy and specific clinical course and high lethality. Numerous damages, the most significant of which are stress, shock, pain, violation of the neurohumoral system involving the mediators of the sympathic part of vegetative nervous system, and pathological condition in which the body as a whole (generalized hypoxia) or region of the body (tissue hypoxia) is deprived of adequate oxygen supply are common in CS. The aim of the research was to study factors, which determine tissue blood and oxygen supply and to detect the possible pathogenetic mechanisms of generalized hypoxia in decompressed tissues and organs in long CS. Systemic arterial pressure, blood supply of skeletal muscles and liver, mesenteric microcirculation, mechanical and chemical resistance of erythrocytes of rats in crash syndrome has been investigated with the use of electrotensometry, H+-clearance, telebiomicroscopy, ultrasound cytolysogram and photoelectrocolorimetry. The model of crush was created by compression of experimental animals' hip during 6 hours. The morphological study of brain tissue during different types of decompression has revealed a contraction of the vessels of the cerebral shell, hypoxia, and perivascular edema. It was stated that interrelated changes play significant role in development of generalized hypoxia of tissues; degree of disorders depend on duration of compression and decompression, reactivity of micro blood vessels; the fall of local hemocirculation in intact tissues and reduced resistance and deformability of erythrocytes takes place.

[Relationship between lipid peroxidation processes, blood vessel wall changes and properties of erythrocytes in pathogenesis of microhemocirculation disorders during experimental diabetes mellitus].

The microhemocirculation play the main role in the development of the micro-and macroangiopathy during Diabetes Mellitus. The aim of our work was to study changes of free-radicals and deformability of erythrocytes using the filtration-photometric and electronic-paramagnetic methods in white rats during Alloxan Diabetes at different stages. Erythrocytes deformability was estimated by the time needed for erythrocytes passage through the filter and state of blood and blood vessels walls were studied by the EPR method. It had been detected that erythrocytes deformability sharply reduced and blood vessels wall was damaged by activation of lipid peroxidation and inhibition of antioxidant system, which may lead to disorders of microhemocirculation.