[Occurrence of rota-I adenoviruses in diarrhea states of children]. / Wystepowanie rota- I adenowirusów w biegunkowych stanach u dzieci.

98 children aged 6 weeks to 5 years with diarrhoea were examined. Rotaviruses and adenoviruses were detected by latex tests Rotalex and Adenolex produced by Orion Diagnostica. Rotaviruses were found in 20.4% of cases, most frequently in children of the age from 6 to 18 months. Adenoviruses were found in 11.2% of cases, most frequently in children of the age from 18 months to 5 years, mainly in mixed infection by- and adenoviruses. Viruses infection were most rare in infants aged from 6 weeks to 6 months. In 4.1% studied cases the coexisting of viral and bacterial infection was observed (K. pneumoniae, E. coli).

[Colonization with group B streptococci in neonates and premature infants from selected neonatal departments]. / Kolonizacja paciorkowcami grupy B noworodków i wczesniaków w wybranych oddzialach noworodków.

Group B streptococci are considered an important etiological agent of sepsis and meningitis in neonates and, particularly, in premature infants. There is a close correlation between colonization with these bacteria and the frequency of symptomatic infection. It is estimated that symptomatic infections occur in 1.0% of colonised neonates. The purpose of this work was to investigate the frequency of neonate colonization with group B streptococci for determination of the risk of symptomatic infection.

[Susceptibility of bacteria isolated from clinical material to ciprofloxacin]. / Wrazliwosc bakterii izolowanych z materialu klinicznego na cyprofloksacyne.

The aim of the study was to evaluate susceptibility to ciprofloxacin of bacteria species isolated from different specimens of clinical materials. The investigated strains (830) were identified using conventional methods. The antibacterial activity of ciprofloxacin was studied by the method of drug dilution in Mueller-Hinton agar. Among aerobic bacteria, only 5 strains (0.6%) were resistant to ciprofloxacin in concentration 4 micrograms/ml; two belonged to E. coli species, two to S. aureus and one was a Streptococcus group B., Corynebacterium sp. MIC90 = 0.12 microgram/ml turned out to be most resistant, next, Gram-negative rods MIC90 = 0.5 microgram/ml, coagulase-negative and positive staphylococci and Neisseria sp. MIC90 = 1.0 microgram/ml. Enterococcus faecalis and anaerobes were the least susceptible to ciprofloxacin. Their MIC90 was 2 and 16 micrograms/ml respectively. Among 20 anaerobic strains, up to 10 were resistant to ciprofloxacin, mainly from Bacteroides and Clostridium genus.

[Occurrence of methicillin-resistant strains in Poland and their characteristics]. / Wystepowanie metycylinoopornych gronkowców w Polsce i ich charakterystyka.

The investigation was performed on 923 strains of S. aureus isolated from clinical material obtained from several regions of Poland. Resistance to methicillin was tested by a dilution method on a solid Mueller-Hinton medium supplemented with 2% of NaCl. Strains exhibiting MIC higher than 4 micrograms/ml were determined as resistant. Resistance to other antibiotics (P, Am, CB, CF, CH, MA, Ge, Bs, E, L, Dx) was tested by a disc method. Bacteriophage typing of S. aureus was performed by a method described by Blair and Williams in RTD and RTD x 100, using basic set of phages and additional phages (88, 89, 187). MRSA were present in various regions of the country with similar frequency (from 3.3% to 8.3%). In one center only the percentage was as high as 52.3%. High percentage of MRSA was noted in burned patients (59.9) and these strains were obtained at one center. Within the MRSA III phage group was dominating as well as non-typable strains and inhibited at 100 x RTD-35.4%. MRSA most frequently were typing with phages 88-40.9%, 89-35.0%, 85-24.0%, and they rarely belonged to the phage group II. Among MRSA strains higher percentage of antibiotic-resistance was noted, as compared with other strains. About 60% of MRSA strains were resistant to 6-8 antibiotics. The dominating resistance concerned penicillins, cephalosporins, aminoglycosides, tetracyclines and erythromycin.

[Susceptibility of methicillin-sensitive and methicillin-resistant staphylococci to ciprofloxacin]. / Wrazliwosc gronkowców metycylino-wrazliwych i metycylino-opornych na ciprofloksacyne.

Investigations were carried on 352 strains of Staphylococcus aureus and 105 strains of coagulase-negative staphylococci, isolated from various clinical materials and derived from some regions of the country. Methicillin-resistance was tested by an antibiotic dilution method in solid Mueller-Hinton medium with addition of 2% NaCl. Staphylococci with MIC higher than 4 micrograms/ml were considered as resistant. The same method for testing resistance to ciprofloxacin was used. Only one strain (S. aureus) was resistant to both ciprofloxacin and methicillin. All remaining strains of staphylococci were ciprofloxacin-susceptible. The MIC for all of them was not higher than 2 micrograms/ml, regardless of resistance to methicillin. Some slight differences in MIC50 and MIC90 values were found between MRSA and MSSA and they were, respectively, 1 microgram/ml and 0.25 microgram/ml, and 2 micrograms/ml and 1 microgram/ml. Mean MIC of ciprofloxacin for MRSA was 1.1 microgram/ml and for MSSA it amounted to 0.4 microgram/ml. Range of MIC was following: MRSA--0.12 microgram/ml--16 micrograms/ml and MSSA--0.12-2 micrograms/ml. Coagulase-negative staphylococci, both methicillin-resistant and methicillin-sensitive, exhibited same value for MIC50, MIC90 and MIC range and they were following: 0.5 microgram/ml, 1 microgram/ml and 0.12-1 micrograms/ml. Both groups differed slightly in mean MIC values which was 0.6 microgram/ml for methicillin-resistant strains and 0.47 microgram/ml for methicillin-sensitive staphylococci. It seems that within staphylococci isolated in Poland there is no correlation between resistance to methicillin and ciprofloxacin, which is frequently pointed out by other authors.

[Role of Staphylococcus saprophyticus in urinary infections]. / Rola Staphylococcus saprophyticus w zakazeniach ukladu moczowego.

A proportion of S. saprophyticus in other coagulase-negative staphylococcal isolates from the urine of patients with urinary infections and healthy individuals has been investigated. Certain diagnostic aspect of the urinary infections with S. saprophyticus have also been considered. Hundred four coagulase-negative staphylococcal strains isolated from patients in Slupsk and Gdansk area and 72 strains of the coagulase-negative staphylococci isolated from the urine of healthy women have been divided into 9 species, according to Kloos and Schleifers' classification. Bacteriologic tests have shown that S. saprophyticus produced 20.4% of the urinary tract infections in Slupsk area holding the second place after S. haemolyticus (27.3%). This species was the most infrequent in the urine of patients in Gdansk area (3.3%). Its sensitivity to antibiotics did not differ from that of other coagulase-negative staphylococci. In contrast to the majority of other strains, S. saprophyticus has not been isolated from the urinary tract of healthy women and has been encountered most frequently in low bacteriuria. Test of resistance to novobiocin which is considered as a simplified identification method of this species proved to be not very precise as other species have also been resistant to this antibiotic.