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1.
J Indian Assoc Pediatr Surg ; 29(3): 292-294, 2024.
Article in English | MEDLINE | ID: mdl-38912014

ABSTRACT

Esophageal atresia (OA) with or without tracheoesophageal fistula affects approximately 1 in 4000 births and commonly presents with polyhydramnios. This appears to be the first report regarding the utility of cervical cerclage with serial amnioreduction to prolong the gestational age of a neonate with OA, thereby improving outcomes for reconstructive surgery.

3.
BMJ Case Rep ; 16(4)2023 Apr 25.
Article in English | MEDLINE | ID: mdl-37185243

ABSTRACT

Hirschsprung's disease (HD) is one of the most well-known gastrointestinal motility disorders. Diagnosis and management of other lesser-known motility disorders are often challenging and tedious. We describe a teenager who was severely constipated from birth and needed intensive care admissions for life-threatening enterocolitis. She also had concomitant anal stenosis. Several rectal biopsies were unable to yield a conclusive diagnosis. Surgical level of resection had to be identified based on the motility of the bowel as determined by transit studies using oral ingestion of a milk feed labelled with Technetium-99m colloid. After completion of all operative stages, histopathological examination of the excised specimens concluded that she had short-segment HD associated with reduced interstitial cells of Cajal in the large bowel. She is currently continent, evacuating voluntarily approximately four times a day and is relieved of all her symptoms.


Subject(s)
Anorectal Malformations , Hirschsprung Disease , Interstitial Cells of Cajal , Female , Adolescent , Humans , Hirschsprung Disease/complications , Hirschsprung Disease/diagnosis , Hirschsprung Disease/surgery , Interstitial Cells of Cajal/pathology , Constriction, Pathologic , Intestine, Large , Intestines
5.
J Indian Assoc Pediatr Surg ; 27(3): 340-344, 2022.
Article in English | MEDLINE | ID: mdl-35733589

ABSTRACT

Purpose: The purpose of this study was to study the presentation of patients who underwent Meckel's diverticulectomy (MD) and utility of pertechnetate Meckel's scan in the diagnosis of MD. Methods: The clinical presentation of a retrospective cohort of patients who underwent MD from January 2007 to December 2019 was studied. The modes of presentation, treatment, and the diagnostic utility of pertechnetate Meckel's scans were evaluated. False-positive and false-negative scans were reviewed. The presence of gastric mucosa on histology of Meckel's was correlated with presentation as gastrointestinal bleeding and positive scan results. Results: Ninety-nine patients underwent MD. Thirty-five out of 263 (13.3%) Meckel's scans done were positive. There was a male preponderance (86.9%). The peak age of presentation was 0-4 years (rectal bleeding or intestinal obstruction). Only a third of the patients with Meckel's diverticulum Meckel's had a preoperative diagnosis of Meckel's. The sensitivity/specificity of Meckel's scan was higher in patients presenting with painless rectal bleeding. Seven patients were false positive (weak tracer uptake or ectopic uptake) and five were false negative. Two patients with false-negative Meckel's scan, having gastrointestinal bleeding had gastric mucosa on histology of Meckel's. Conclusion: Meckel's diverticulum has a male predominance. Meckel's scan has a high sensitivity in the children presenting with fresh painless rectal bleeding but is of limited use in the diagnosis of Meckel's diverticulum in other forms of presentations. False-positive scans can be anticipated in the presence of weak or ectopic uptake. False-negative scans can occur even in the presence of bleeding and in spite of the presence of gastric mucosa in the Meckel's diverticulum. Laparoscopy is a useful tool in diagnosis and treatment.

6.
Indian J Pediatr ; 89(10): 1010-1012, 2022 10.
Article in English | MEDLINE | ID: mdl-35119646

ABSTRACT

Acute abdomen in the fetus can present as nonspecific fetal distress, and occasionally necessitate emergency cesarean section for the mother and subsequent emergency neonatal surgery. Often, preoperative diagnosis in utero is challenging. The authors herein report two cases, a case of primary segmental antenatal intestinal volvulus resulting in the mother presenting at 35 wk with symptoms and decreased fetal movements, and another case of a rapidly distending fetal abdominal mass at 31 wk manifesting as fetal hydrops. The postdelivery newborn management issues are presented. Early identification by the maternal and neonatal team allows for early neonatal surgery and can reduce morbidity and mortality. This can be facilitated by advanced maternal and child health services and efficient patient transport systems.


Subject(s)
Premature Birth , Cesarean Section , Child , Emergencies , Female , Fetus , Humans , Infant, Newborn , Pregnancy , Prenatal Care/methods
7.
ANZ J Surg ; 92(5): 1159-1164, 2022 05.
Article in English | MEDLINE | ID: mdl-35072328

ABSTRACT

INTRODUCTION: Posterior urethral valve (PUV) is the most common congenital cause of bladder outflow obstruction in male infants. Despite timely treatment, renal damage can still occur in the long-term leading to chronic kidney disease (CKD). METHODS: A retrospective review of all PUV patients in a single tertiary institution between April 1998 and July 2019 was conducted to analyze their presentations, management and outcomes. Long-term renal function, radiologic scans and somatic growth were evaluated. RESULTS: A total of 16 patients were included in this study. Two patients who defaulted all follow-ups were excluded. Seven patients (43.7%) presented in the antenatal period; four patients (25%) presented in the neonatal period and five patients (31.3%) presented in the post-neonatal period. Primary transurethral fulguration of valves was done in 13 patients, while three had vesicostomies as the primary procedure. Three patients had associated anterior urethral valves (AUV), which were treated endoscopically. Nine boys had additional procedures for diversion and undiversion, VUR, non-functioning kidney and clean intermittent catheterization. Ten patients had urodynamic studies performed, of which eight patients received anticholinergic therapy. Eleven patients had DMSA scans, of which three patients had a normal study and eight patients showed unilateral reduced function. Four patients were diagnosed with CKD on long-term follow-up duration over 5 years. All patients were shown to have good somatic growth. CONCLUSION: Patients with PUV can suffer from complications despite primary treatment. In our small cohort, a quarter of our patients developed CKD on follow-up. Thus, patients need long-term follow-up to optimize bladder and renal function.


Subject(s)
Renal Insufficiency, Chronic , Urethra , Female , Humans , Infant , Infant, Newborn , Male , Pregnancy , Retrospective Studies , Singapore/epidemiology , Tertiary Care Centers , Urethra/surgery , Urinary Bladder
8.
Int J Biol Macromol ; 118(Pt A): 168-179, 2018 Oct 15.
Article in English | MEDLINE | ID: mdl-29924984

ABSTRACT

Enzymatic dehairing as a part of the efforts for greener leather processing has reached progressive advancement with the tradition-bound tanning industry being now more receptive to cleaner processing methods due to increasing pressure from environmental groups. The dehairing mechanism is vaguely understood at present from the point of view of the enzyme specificity, which is needed for consistent and satisfactory hair removal without deleterious effect on the leather quality. Gaining insight into the dehairing specificity would help in designing efficient dehairing process. This paper attempts to review the literature pertaining to all the relevant and critical issues in detail to clearly delineate the right kind of substrate specificity required to attack only the potential targets for hair removal, and for making fine quality leather without adverse effect on other desired leather making components of the skin matrix. The gap in understanding of these critical issues is discussed with recommendation for further scientific studies in the area.


Subject(s)
Enzymes/chemistry , Hair Removal , Peptide Hydrolases/chemistry , Enzyme Therapy , Humans , Peptide Hydrolases/therapeutic use , Substrate Specificity
9.
J Pediatr Urol ; 14(1): 51.e1-51.e7, 2018 02.
Article in English | MEDLINE | ID: mdl-28927720

ABSTRACT

INTRODUCTION: Congenital pelviureteric junction obstruction (PUJO) is one of the most frequent causes of neonatal hydronephrosis. Obstruction at the PUJ has potential severe adverse outcomes, such as renal damage. While pyeloplasty has been established as the definitive treatment, the exact pathophysiology of congenital PUJO remains unknown. Recent research has proposed neuronal innervation defects as an etiological factor in congenital PUJO. We aim to study the expression of various neuronal markers in PUJO specimens compared with controls, and evaluate whether severity of renal disease or dysfunction pre-operatively is related to expression of neuronal markers in resected PUJO specimens. MATERIALS AND METHODS: All consecutive patients who underwent dismembered pyeloplasty at KK Women's and Children's Hospital, Singapore, for intrinsic PUJO from 2008 to 2012 were included. Patients with other co-occurring renal pathologies were excluded. Controls were obtained from nephrectomy patients with Wilm's tumor or other benign renal conditions during the same period. Specimens were stained immunohistochemically with neuronal markers protein gene product 9.5 (PGP9.5), synaptophysin, and S-100, and with CD-117, a marker for interstitial cells of Cajal (Table). Levels of expression of the markers were assessed semiquantitatively (decreased, increased or no change) in comparison with controls by two independent observers. Pre-operative data of patients' renal anatomical (ultrasonography measurements of renal pelvis size) and functional parameters (differential renal function measured using MAG-3 renal scans) were obtained. DISCUSSION: Thirty-eight PUJO specimens (38 renal units) and 20 controls were studied. Mean patient age at pyeloplasty was 25.3 months (2.9-167.6 months). Median pre-operative pelvic size was 25.0 mm (17.0-50.0 mm). Both PUJO specimens and controls showed great heterogeneity in distribution of innervation. All four immunohistochemical markers were not predictive of significant pre-operative renal pelvis dilation or pre-operative diminished renal function of the operated kidney. CONCLUSIONS: There exists marked variability in expression of neuronal markers synaptophysin, PGP9.5, and S-100, and CD-117 in PUJO specimens compared with controls. Our results show no clinical significance of the expression of neuronal markers in predicting degree of pre-operative renal pelvis dilation or differential renal function. The heterogeneity of expression of neuronal markers in PUJO specimens and controls in our population is at variance with prior studies. The etiology of PUJO is likely to be complex and multifactorial.


Subject(s)
Hydronephrosis/congenital , Kidney Pelvis/surgery , Multicystic Dysplastic Kidney/etiology , Multicystic Dysplastic Kidney/pathology , Multicystic Dysplastic Kidney/surgery , Ubiquitin Thiolesterase/metabolism , Ureteral Obstruction/etiology , Ureteral Obstruction/pathology , Ureteral Obstruction/surgery , Biomarkers/metabolism , Biopsy, Needle , Case-Control Studies , Child, Preschool , Female , Humans , Hydronephrosis/diagnostic imaging , Hydronephrosis/etiology , Hydronephrosis/pathology , Hydronephrosis/surgery , Immunohistochemistry , Infant , Laparoscopy/methods , Male , Multicystic Dysplastic Kidney/diagnostic imaging , Neural Conduction , Reference Values , S100 Proteins/metabolism , Severity of Illness Index , Synaptophysin/metabolism , Treatment Outcome , Ultrasonography, Doppler , Ureteral Obstruction/diagnostic imaging
11.
J Pediatr Surg ; 51(8): 1255-61, 2016 Aug.
Article in English | MEDLINE | ID: mdl-26850908

ABSTRACT

AIM: The role of ischemia in the pathogenesis of necrotizing enterocolitis (NEC) remains unclear. We used immunohistochemical markers of hypoxia to identify presence/absence of ischemia in NEC and spontaneous intestinal perforation (SIP) with clinical correlation. METHODS: Immunohistochemical staining was performed on 24 NEC and 13 SIP intestinal resection specimens using 2 hypoxia markers, hypoxia inducible factor 1α (HIF-1α) and glucose transporter 1 (GLUT1) and inflammatory markers, leukocyte common antigen (LCA) and myeloperoxidase. Ischemic score (0-6) from the sum of the HIF-1α and GLUT1 staining intensity grades was devised (positive ≥3). Inflammation was graded from the sum of LCA and myeloperoxidase grading. Relevant clinical information was obtained from hospital case records. RESULTS: Fourteen NEC specimens had positive ischemic score (4.6±1.2). The remaining 10 NEC (ischemic score 0.7±0.8) and all 13 SIP samples (ischemic score 0.5±0.5) were ischemic-negative. The ischemic-positive cases had classic NEC with multiple areas of bowel necrosis; were associated with later onset, enteral feeding and pneumatosis. In contrast, all ischemic-negative NEC were short-segment NEC with perforation. Their clinical profile was similar to the SIP cases with younger gestational age at birth, early onset, association with ibuprofen/indomethacin usage but not with feeding and pneumatosis. Ischemic scores are correlated with inflammation scores in mucosa but not submucosa. CONCLUSIONS: Ischemia as assessed with immunohistochemical markers HIF-1α and GLUT1, has a primary role in pathogenesis of classic NEC only, not in SIP or short-segment NEC with perforation. Better categorization of the different types of NEC can direct appropriate prevention and treatment strategies.


Subject(s)
Enterocolitis, Necrotizing/etiology , Ischemia/complications , Age of Onset , Biomarkers/analysis , Enterocolitis, Necrotizing/surgery , Glucose Transporter Type 1/analysis , Humans , Hypoxia/diagnosis , Hypoxia-Inducible Factor 1, alpha Subunit/analysis , Immunohistochemistry , Indomethacin , Infant , Infant, Newborn , Intestinal Perforation/etiology , Intestines/chemistry , Intestines/pathology , Ischemia/diagnosis
12.
Singapore Med J ; 56(5): e82-4, 2015 May.
Article in English | MEDLINE | ID: mdl-26034325

ABSTRACT

Rectovestibular fistula is the most common type of anomaly found in a female newborn with anorectal malformation. However, when the baby is found to have two orifices in the introitus, rectovaginal fistula is much less common and suspected. The rare differential diagnosis of Müllerian agenesis, a condition in which the rectum shifts anteriorly and the vagina is absent, is seldom considered. In many cases, the diagnosis of Müllerian agenesis is made only during definitive anorectoplasty. In view of its impact on management, a proper examination under anaesthesia, imaging studies and a diagnostic laparoscopy may be required to confirm the presence or absence of Müllerian structures in such patients. We herein describe a patient with the rare coexistence of VACTERL association and Müllerian agenesis, and discuss the management of anorectal malformations in female patients with Müllerian agenesis.


Subject(s)
Anus, Imperforate/diagnosis , Mullerian Ducts/abnormalities , Vagina/abnormalities , Abnormalities, Multiple/diagnosis , Anal Canal/abnormalities , Anal Canal/surgery , Anorectal Malformations , Anus, Imperforate/complications , Anus, Imperforate/surgery , Child , Diagnosis, Differential , Esophagus/abnormalities , Female , Heart Defects, Congenital/complications , Humans , Infant, Newborn , Kidney/abnormalities , Laparoscopy , Limb Deformities, Congenital/complications , Rectal Fistula/diagnosis , Rectum/abnormalities , Rectum/surgery , Spine/abnormalities , Trachea/abnormalities
15.
J Pediatr Surg ; 48(11): 2336-42, 2013 Nov.
Article in English | MEDLINE | ID: mdl-24210209

ABSTRACT

BACKGROUND: Excision has been established as a standard management practice for choledochal cysts in the last few decades. The two most commonly performed methods of reconstruction after excision are hepaticoduodenostomy (HD) and Roux-en-Y hepaticojejunostomy (HJ), of which the HJ is favored by most surgeons. Evidence concerning the optimal method of reconstruction is, however, sparse. MATERIALS AND METHODS: Studies comparing outcomes from HD and HJ after choledochal cyst excision were identified by searching Medline, Ovid, Search Medica, Elsevier Clinicalkey, Google Scholar and Cochrane library. Suitable studies were chosen and data extracted for meta-analysis. Outcomes evaluated included operative time, hospital stay and incidence of postoperative bile leak, cholangitis, reflux/gastritis, anastomotic stricture, bleeding, intestinal obstruction and re-operative rate. Pooled odds ratios (OR) were calculated for dichotomous variables; pooled mean differences (MD) were measured for continuous variables. RESULTS: Six retrospective studies were included in this meta-analysis, comprising a total of 679 patients, 412 of whom (60.7%) underwent HD, and the remainder, 267 (39.3%) underwent HJ. Although, HD group had slightly shorter hospital stay (MD: 0.30; 95% CI: -0.22-0.39; P < 0.00001) it showed a higher incidence of postoperative reflux/gastritis (OR: 0.08; 95% CI: -0.02-0.39; P = 0.002). However, the other outcomes such as bile leak, cholangitis, anastomotic stricture, bleeding, operative time, reoperation rate and adhesive intestinal obstruction did not differ between HD and HJ groups. CONCLUSIONS: HD shows higher postoperative reflux/gastritis than HJ but a shorter hospital stay. There are few good-quality studies that compare the outcomes from HD and HJ, meaning that caution should be exercised in the generalization of the results of this meta-analysis, which suggests HD to be comparable with HJ in terms of other complications, operative benefits and outcomes.


Subject(s)
Choledochal Cyst/surgery , Duodenostomy/methods , Jejunostomy/methods , Liver/surgery , Anastomosis, Roux-en-Y , Anastomotic Leak/epidemiology , Anastomotic Leak/etiology , Bile , Cholangitis/epidemiology , Cholangitis/etiology , Constriction, Pathologic , Duodenostomy/statistics & numerical data , Gastritis/epidemiology , Gastritis/etiology , Humans , Intestinal Obstruction/epidemiology , Intestinal Obstruction/etiology , Jejunostomy/statistics & numerical data , Laparoscopy/methods , Laparoscopy/statistics & numerical data , Length of Stay/statistics & numerical data , Operative Time , Postoperative Complications/epidemiology , Postoperative Complications/etiology , Reoperation/statistics & numerical data , Retrospective Studies , Treatment Outcome
18.
J Pediatr Surg ; 48(3): 642-51, 2013 Mar.
Article in English | MEDLINE | ID: mdl-23480925

ABSTRACT

AIM: Transanal endorectal pull-through (TERPT) has become popular for single-stage treatment of Hirschsprung's disease. The benefits of TERPT over the conventional transabdominal approach (TAB) are still unclear. We performed a comprehensive meta-analysis comparing the clinical outcomes of TERPT and TAB. METHODS: Original articles published from 1998 to 2012 were searched from Medline, Embase, and Cochrane databases. Randomized controlled trials (RCT) and observational clinical studies (OCS) comparing TERPT and TAB were included. Outcomes evaluated included operative time, hospital stay and incidence of postoperative incontinence/soiling, constipation and enterocolitis. Pooled odds ratios (OR) were calculated for dichotomous variables; pooled mean differences (MD) were measured for continuous variables. RESULTS: Of 93 studies, 1 RCT and 11 OCS were included, comprising 444 cases of TERPT and 348 cases of TAB (215 Soave, 94 Duhamel, 24 Swenson, 15 Rehbein procedures). TERPT had shorter operative time (MD=-57.85 min; 95% confidence interval [CI], -83.11 to -32.60; P<0.00001) and hospital stay (MD=-7.06 days; 95% CI, -10.95 to -3.16; P=0.0004). TERPT had less postoperative incontinence/soiling (OR=0.58; 95% CI 0.37-0.90; P=0.01) and constipation (OR=0.49; 95% CI 0.30-0.81; P=0.005). There was no difference in incidence of postoperative enterocolitis. CONCLUSION: TERPT is superior to TAB in operative time, hospital stay, postoperative incontinence and constipation. However, more randomized controlled trials are necessary to verify the benefit of TERPT for Hirschsprung's disease.


Subject(s)
Hirschsprung Disease/surgery , Abdomen , Anal Canal , Child , Digestive System Surgical Procedures/methods , Humans , Rectum
19.
Childs Nerv Syst ; 29(4): 609-19, 2013 Apr.
Article in English | MEDLINE | ID: mdl-23207975

ABSTRACT

BACKGROUND: Multiple neural tube defects (MNTDs) are a rare occurrence. Although the clinical incidence is small, MNTDs raise some interesting embryological queries. AIM: This study aims to investigate the morphological and clinical variations observed in neonates presenting with multiple neural tube defects and associated central nervous system anomalies. MATERIALS AND METHODS: This is a prospective study carried out at our institute to assess clinical and morphological variation in patients presenting with multiple neural tube defects. RESULTS: Among the 263 patients with a neural tube defect, who presented to our outpatient department and emergency departments, only 10 cases of MNTDs were identified. Thus, incidence of MNTDs in the cohort of patients affected with NTD was 0.038 %. Among the 10 patients, 9 had double neural tube defects and 1 patient had three neural tube defects. CONCLUSIONS: Multiple neural tube defects may not be very rare in the general population, especially the populations with high incidence of neural tube defects. Multisite closure theory has the versatility to explain various combinations of neural tube defects, but better insights into the molecular pathways governing this tightly regulated process can provide us the missing link in establishing the pathogenesis of multiple NTDs. It can also provide us with an opportunity to prevent NTDs or treat them in utero by pharmacological modulation of these signaling pathways.


Subject(s)
Neural Tube Defects/epidemiology , Female , Humans , Incidence , Infant , Infant, Newborn , Male , Neural Tube Defects/pathology , Neural Tube Defects/surgery , Prospective Studies
20.
J Pediatr Surg ; 47(12): 2316-20, 2012 Dec.
Article in English | MEDLINE | ID: mdl-23217897

ABSTRACT

Non-operative management of isolated blunt hepatic trauma is recommended except when hemodynamic instability requires immediate laparotomy. Hepatic artery angioembolization is increasingly used for hepatic injuries with ongoing bleeding as demonstrated by contrast extravasation on the CT scan. It is used primarily or after laparotomy to control ongoing hemorrhage. Hepatic angioembolization as part of multimodality management of hepatic trauma is reported mainly in adults, with few pediatric case reports. We describe our institution experience with primary pediatric hepatic angioembolization and review the literature with regard to indications and complications. Two cases (3 and 8 years old), with high-grade blunt hepatic injuries with contrast extravasation on the CT scan were successfully managed by emergency primary hepatic angioembolization with minimal morbidity and avoided laparotomy. To date, the only reports of pediatric hepatic angioembolization for trauma are 5 cases for acute bleeding and 15 delayed cases for pseudoaneurysm. The role of hepatic angioembolization in the presence of an arterial blush on CT in adults is accepted, but contested in a pediatric series, despite higher transfusion rate and mortality rate. We propose that hepatic angioembolization should be considered adjunct treatment, in lieu of, or in addition to emergency laparotomy for hemostasis in pediatric blunt hepatic injury.


Subject(s)
Embolization, Therapeutic/methods , Liver/injuries , Wounds, Nonpenetrating/therapy , Child , Child, Preschool , Emergency Service, Hospital , Follow-Up Studies , Humans , Injury Severity Score , Liver/diagnostic imaging , Male , Risk Assessment , Tomography, X-Ray Computed/methods , Treatment Outcome , Wounds, Nonpenetrating/diagnostic imaging , Wounds, Nonpenetrating/physiopathology
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