ABSTRACT
A new reversed-phase, isocratic LC method was developed for the quantitative determination of COX-2 inhibitor celecoxib in bulk drugs and in pharmaceutical dosages. The proposed method is also applicable for the purity evaluation of celecoxib in bulk drugs. 5-Methyl 2-Nitro phenol has been used as internal standard for the quantitative determination of celecoxib. The method has been completely validated and proven to be rugged. The limit of detection (LOD) and limit of quantitation (LOQ) for celecoxib impurities namely, 4-hydrazino benzene sulfonamide (Intermediate I) and 1-(4-methyl phenyl)-4,4.4-trifluro butan-1,3-dione (Intermediate II) were found to be 32.0 and 97 ng. respectively. The active pharmaceutical ingredient was extracted from its finished dosage form (capsule) using methanol. The percentage recoveries ranged from 90.7 to 93.8. The stability studies were performed for celecoxib solution placed on laboratory bench and in refrigerator for hundred days. The samples were found to be stable for the study period.
Subject(s)
Cyclooxygenase Inhibitors/analysis , Isoenzymes/metabolism , Prostaglandin-Endoperoxide Synthases/metabolism , Sulfonamides/analysis , Capsules , Celecoxib , Chromatography, High Pressure Liquid , Cyclooxygenase 2 , Cyclooxygenase 2 Inhibitors , Drug Contamination , Drug Stability , Hydrogen-Ion Concentration , Pyrazoles , Reproducibility of Results , SolutionsABSTRACT
Two isocratic liquid chromatography (LC) methods have been developed for the purity estimation and quantitative determination of sibutramine HCl, using 4-chloro aniline and lovastatin as internal standards, respectively. The precision has been checked in terms of F-test variance ratio using latter method as reference. The ratio of variances of the two methods is close to unity, confirming their good precision. The correlation coefficient for linear regression is more than 0.999. The inter and intra-day precision is found to be < 1.3% RSD. The accuracy determined as relative mean error (RME) for the intra-day assay is +/- 1.7%. The enantiomeric separation of sibutramine by chiral chromatography method has been described also. This method is capable of separating the two enantiomers with a selectivity of 1.4 and a resolution of 4.0. Both methods are found to be stability indicating and useful in the quality control of the bulk material.
Subject(s)
Chromatography, Liquid/methods , Cyclobutanes/isolation & purification , Antidepressive Agents/isolation & purification , Drug Stability , Quality Control , Reference Standards , Reproducibility of Results , StereoisomerismABSTRACT
The role of marketing in a health care facility has been a focus of discussion during the past decade. Varied opinions have been expressed about the purpose and contribution of marketing within the health care industry. The authors empirically investigate (1) the extent of marketing orientation in hospitals, (2) the degree of marketing orientation as it relates to hospital characteristics, and (3) the relationship of marketing orientation to hospital performance.
Subject(s)
Hospital Administration/statistics & numerical data , Marketing of Health Services/statistics & numerical data , Evaluation Studies as Topic , Organizational Culture , Surveys and Questionnaires , United StatesABSTRACT
It is about two decades since the marketing scholars identified the need and relevance of marketing in health care organizations. The health care industry itself has been undergoing significant changes brought on by deregulation, declining demand, intraorganizational competition and growth of HMO's and PPO's. Yet, the industry has not fully implemented the marketing concept. For many health care organizations, marketing seems still to mean advertising and public relations as evidenced by an empirical study.
