ABSTRACT
Background and objective The main goal of root canal treatment is to eliminate microorganisms from the canal and to prevent re-infection. To achieve these goals, instrumentation must be combined with adequate irrigation and the placement of intracanal medicament. This study aims to compare the effect of different irrigation activation techniques, i.e., passive ultrasonic irrigation (PUI), EndoActivator (EA), and conventional needle irrigation on the penetration of calcium hydroxide (CH), an intracanal medicament into dentinal tubules. Methodology A total of 60 single-rooted extracted human teeth were selected, which were de-coronated to standardize the root length of 12 mm. An access cavity was prepared, and biomechanical preparation was done. The samples were randomly assigned to three experimental groups: Group I received conventional needle irrigation, Group II underwent EA irrigation, and Group III was subjected to PUI. All the samples were filled with CH paste mixed with Rhodamine B dye, and the orifice openings were sealed with Cavit. The samples were stored in an incubator for 24 hours and were subsequently sectioned horizontally at the coronal, middle, and apical thirds using a hard tissue microtome. These were observed under a confocal laser scanning microscope to evaluate the depth of medicament penetration. Statistical analysis was performed using IBM SPSS Statistics for Windows, Version 25.0 (IBM Corp., Armonk, NY). The Shapiro-Wilk test was employed to assess normality, while ANOVA and Tukey's post hoc analysis were utilized to determine significance. Results It was observed that the depth of penetration of CH into dentinal tubules was highest in Group III (PUI), followed by Group II (EA irrigation), with the least penetration observed in Group I (conventional needle irrigation). All the groups showed maximum penetration at the coronal third followed by the middle and apical third. Conclusions Passive ultrasonic irrigant activation resulted in more penetration of CH into the dentinal tubules followed by EA irrigant activation.
