ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of 20% fluor-hydroxyapatite (FHA) on proliferation and osteogenic differentiation of human MG63 osteosarcoma cells.</p><p><b>METHODS</b>FHA was prepared by chemical precipitation method, and its structure and surface features were tested by scanning microscope, X-ray diffraction (XRD) and Fourier transform infrared spectroscopy. MG63 cells were cultured and divided into FHA, hydroxyapatite (HA) and control groups (n = 3). The proliferation of the cells was evaluated using methylthiazol tetrazolium (MTT) assay. ALP activity of the cells was assessed. Osteogenic differentiation was evaluated based on the reverse transcription PCR (RT-PCR) of differentiation-related genes, namely, collagen type I (Col I), alkaline phosphatase (ALP), osteocalcin (OCN) and core binding factor α1 (Cbfα1). The data were analyzed statistically by one-way analysis of variance using SPSS 13.0 software.</p><p><b>RESULTS</b>XRD test showed that the main crystalline phase of FHA was similar to that of HA. Absorptance value of cells exposed to FHA(1.87±0.06) measured by MTT was higher than that of the control(1.25±0.02) on the third day(P < 0.05), and there was no statistically significant difference between the cells exposed to FHA and HA(1.84±0.03) (P > 0.05). ALP activity of the cells exposed to FHA(4.62±0.09)was higher than that of the control (1.92 ± 0.05) (P < 0.05). RT-PCR tests showed that compared with the control, FHA up-regulated the expression of Col I, ALP and OCN mRNA, down-regulated the expression of Cbfα1 mRNA.</p><p><b>CONCLUSIONS</b>FHA enhances the proliferation and osteogenic differentiation-related gene expression, and has good biocompatibility.</p>
Subject(s)
Humans , Alkaline Phosphatase , Genetics , Metabolism , Analysis of Variance , Biocompatible Materials , Bone Neoplasms , Metabolism , Pathology , Cell Differentiation , Genetics , Cell Proliferation , Physiology , Collagen Type I , Genetics , Metabolism , Core Binding Factor Alpha 1 Subunit , Genetics , Durapatite , Chemistry , Pharmacology , Hydroxyapatites , Osteocalcin , Genetics , Metabolism , Osteogenesis , Osteosarcoma , Metabolism , Pathology , Spectroscopy, Fourier Transform Infrared , X-Ray DiffractionABSTRACT
In the present study, novel biomemetic composite scaffolds using the sol-gel derived bioactive glass (BG), collagen (COL), and phosphoserine (PS) were prepared by freeze-drying. MC3T3-E1 were cultivated in vitro, collected and seeded onto the surface of BG, BG-COL and BG-COL-PS. Cell attachment and proliferation were observed. The cell proliferation was tracked by MTT method 1,3,5 d after seeding. MTT showed that the cells can adhere to and proliferate well on the surface of the scaffolds, and the cell proliferation result of scaffold BG-COL-PS was better than those of scaffolds BG and BG-COL. Therefore, the scaffold BG-COL-PS can be a potential scaffold for tissue engineer.
