Pediatric palliative care for metabolic diseases: 20-year epidemiological survey of outpatients at a Brazilian quaternary hospital.

The interface between pediatric palliative care (PPC) and inborn metabolic diseases (IMD) remains incipient, though these conditions fill the state of art of complex chronic diseases, eligible to this health approach. We analyzed the medical records of PPC clinic during the years 2001 to 2021 and the IMD outpatients. We established a parallel with the world scientific literature concerning the epidemiology of PPC and IMD. Among outpatients, 14% were diagnosed with IMD, which were referred to the PPC service earlier compared to Non-IMD cases. The Group 3 (complex molecules) was the most frequent (64.7%), following by Group 1 representing by small molecules (21.6%), the latter having a lower median age at diagnosis when compared to the former (0.7 vs. 5.2 years, p = 0.001). The sphingolipidoses were the pathologies most frequent in our cohort, in line with what was observed in the literature. There were no differences between IMD groups in terms of diagnosis and PPC referral age, however in Non-IMD conditions, the age of diagnosis were earlier than IMD. Nevertheless, IMD group showed lower age of referral to PPC. The IMD comprises large fraction of outpatients in the PPC setting, thus further studies are needed in this field.

Epidemiological Assessment of a Pediatric Palliative Care Clinic at a Brazilian Quaternary Hospital: 20 Years of Experience.

Background: The pediatric palliative care (PPC) sets up an interdisciplinary approach of chronic complex diseases throughout birth to adolescence. It encompasses countless contrasts in development and diagnosis scopes, which make this area a challenge to nonpediatric practitioners. Objective: We sought to assess the most prevalent diseases in follow-up of the PPC team. Methods: We analyzed the medical records of PPC clinic during the years 2001 and 2021 and the diagnosis of outpatients. We established a parallel with the world scientific literature concerning the epidemiology of PPC. Results: The most prevalent diseases were epidermolysis bullosa (36.9%), followed by neurological Inherited Errors of Metabolism (IEM) diseases (19.0%), IEM diseases (14.3%), dysmorphological and chromosomal disorders (8.5%), skeletal disorders mainly osteogenesis imperfecta (6.9%), and liver transplantation conditions (5.5%) (p < 0.001). The less frequent conditions were external causes, such as neonatal insults or traffic accidents (2.8%), cancer (1.7%), congenital cardiopathies (1.4%), congenital infectious diseases (1.1%), gastrointestinal and hepatic conditions (0.8%), and rheumatological conditions (0.3%). The patients were older at diagnosis (6.9 years) and at PPC referral (13.2 years) than patients with epidermolysis bullosa and skeletal disorders and dysmorphological and chromosomal disorders were younger on referral. Conclusion: There are a lot of complex chronic conditions which could benefit from palliative care in pediatric setting. However, epidemiological and symptomatological assessment of the health service is necessary to provide an appropriate care to the country's reality.

Diversity and distribution of iron-oxidising bacteria belonging to Gallionellaceae in different sites of a hydroelectric power plant.

Iron (Fe) is the fourth most abundant element on the planet, and iron-oxidising bacteria (FeOB) play an important role in the biogeochemical cycle of this metal in nature. FeOB stands out as Fe oxidisers in microaerophilic environments, and new members of this group have been increasingly discussed in the literature, even though their isolation can still be challenging. Among these bacteria is the Gallionellaceae family, mainly composed of neutrophilic FeOB, highlighting Gallionella ferruginea, and nitrite-oxidiser genera. In the previous metagenomic study of the biofilm and sediments of the cooling system from the Irapé hydroelectric power plant (HPP-Irapé), 5% of the total bacteria sequences were related to Gallionellaceae, being 99% unclassified at genus level. Thus, in the present study, a phylogenetic tree based on this family was constructed, in order to search for shared and unique Gallionellaceae signatures in a deep phylogenetic level affiliation and correlated them with geomorphologic characteristics. The results revealed that Gallionella and Ferrigenium were ubiquitous reflecting their ability to adapt to various locations in the power plant. The cave was considered a hotspot for neutrophilic FeOB since it harboured most of the Gallionellaceae diversity. Microscopic biosignatures were detected only in the CS1 sample, which presented abundance of the stalk-forming Ferriphaselus and of the sheath-forming Crenothrix. Further studies are required to provide more detailed insights on Gallionellaceae distribution and diversity patterns in hydroelectric power plants, particularly its biotechnological potential in this industry.

Anti-Trypanosoma cruzi Potential of Vestitol Isolated from Lyophilized Red Propolis.

Chagas disease (CD) is a worldwide public health problem, and the drugs available for its treatment have severe limitations. Red propolis is a natural extract known for its high content of phenolic compounds and for having activity against T. cruzi. The aim of this study was to investigate the trypanocidal potential of red propolis to isolate, identify, and indicate the mode of action of the bioactive compounds. The results revealed that the total phenolic content was 15.4 mg GAE/g, and flavonoids were 7.2 mg QE/g. The extract was fractionated through liquid-liquid partitioning, and the trypanocidal potential of the samples was evaluated using the epimastigote forms of the Y strain of T. cruzi. In this process, one compound was characterized by MS, 1H, and 13C NMR and identified as vestitol. Cytotoxicity was evaluated employing MRC-5 fibroblasts and H9C2 cardiomyocytes, showing cytotoxic concentrations above 15.62 µg/mL and 31.25 µg/mL, respectively. In silico analyses were applied, and the data suggested that the substance had a membrane-permeation-enhancing effect, which was confirmed through an in vitro assay. Finally, a molecular docking analysis revealed a higher affinity of vestitol with farnesyl diphosphate synthase (FPPS). The identified isoflavan appears to be a promising lead compound for further development to treat Chagas disease.

Complete genome sequences of five Escherichia coli strains with probiotic attributes.

The complete genome sequences of five Escherichia coli strains with probiotic attributes were determined, including strain A0 34/86, a component of the probiotic product Colinfant New Born, and strains H22, 582, B771, and B1172 with published probiotic potential. The size of sequenced genomes ranged from 5,092 to 5,408 kb.

Anti-Inflammatory, Antimicrobial, Antioxidant and Photoprotective Investigation of Red Propolis Extract as Sunscreen Formulation in Polawax Cream.

Many activities have been described for propolis, including, antiviral, antibacterial, antifungal, anti-inflammatory, immunoregulatory, antioxidant and wound healing properties. Recently, propolis has been highlighted due to its potential application in the pharmaceutical and cosmetic industries, motivating a better understanding of its antioxidant and anti-inflammatory activities. Propolis and its main polyphenolic compounds presented high antioxidant activity, and effectiveness as broad spectrum UVB and UVA photoprotection sunscreens. Through a qualitative phytochemical screening, the ethanolic red propolis extracts (EEPV) (70% at room temperature and 70% at a hot temperature) presented a positive result for flavonoids and terpenoids. It presented an antioxidant activity for reducing 50% of DPPH of 17 and 12 µg/mL for extraction at room temperature and at a hot temperature, respectively. The UPLC-QTOF-MS/MS analysis allowed the annotation of 40 substances for EEPV-Heated and 42 substances for EEPV-Room Temperature. The IC50 results of the ABTS scavenging activity was 4.7 µg/mL for both extractions, at room temperature and at a hot temperature. Additionally, we also evaluated the cytotoxic profile of propolis extracts against macrophage (RAW 264.7 cells) and keratinocytes (HaCaT cells), which showed non-cytotoxic doses in cell viability assays even after a long period of exposure. In addition, propolis extracts showed antibacterial activity for Gram-positive bacteria (Staphylococcus aureus and Staphylococcus epidermidis), demonstrating potential biological activity for the creation of formulations aimed at disease control and prevention.

Desafios e proposições para a avaliação psicológica com grupos minorizados: (des)alinhamentos sociopolíticos / Challenges and propositions for psychological assessment with minority groups: sociopolitical (mis)alignments / Desafíos y propuestas para la evaluación psicológica con grupos minoritarios: (Des)ajustes sociopolíticos

Este trabalho visa discutir desafios e proposições relacionados à avaliação psicológica e pesquisa com grupos minorizados no Brasil. Para tanto, o conteúdo da seção Participantes de todos os artigos publicados na Revista Avaliação Psicológica entre 2011 e 2021 foi agregado, gerando um corpus analisado pelo software Iramuteq. Os resultados da Classificação Hierárquica Descendente indicaram a existência de quatro classes, que enfatizam informações de gênero e idade (Classe 1), regional e educacional (Classe 2), dados interseccionais (Classe 3) e ênfase nosológica (Classe 4). Foram visibilizados os estudos que apresentaram maior detalhamento nos dados sociodemográficos. Conclui-se que existe a necessidade de ampliar as informações divulgadas de forma a visibilizar grupos minorizados socialmente. Para tanto, são apresentadas propostas que visam contribuir com a visibilização desses grupos, de acordo com a legislação vigente do Conselho Federal de Psicologia, e com o cruzamento de informações relevantes para a construção de um conhecimento interseccional na área. (AU)

This work aims to discuss challenges and propositions related to psychological assessment and research with minority groups in Brazil. For this, the content of the Participants section of all articles published in the Revista Avaliação Psicológica between 2011 and 2021 generated a corpus for analysis using the IRaMuTeQ software. Results of the Descending Hierarchical Classification indicated the existence of four classes, which emphasized information on gender and age (Class 1), regional and educational data (Class 2), intersectional data (Class 3) and nosological information (Class 4). Studies that presented more detailed sociodemographic data are discussed. The conclusion highlights the need to expand the information disclosed to make social minority groups visible. Accordingly, proposals are presented that aim to contribute to the visibility of these groups, in accordance with the current legislation of the Conselho Federal de Psicologia. Relevant information for the construction of intersectional knowledge in the area is also presented. (AU)

Este trabajo busca discutir desafíos y propuestas relacionadas con la evaluación psicológica de grupos minoritarios en Brasil. Para ello, se agregó el contenido de la sección Participantes de todos los artículos publicados en la Revista Avaliação Psicológica entre 2011 y 2021, generando un corpus analizado por el software Iramuteq. Los resultados de la Clasificación Jerárquica Descendente indicaron que existen cuatro clases, que enfatizan informaciones de género y edad (Clase 1), de regional y educativa (Clase 2), datos interseccionales (Clase 3) y énfasis nosológico (Clase 4). Se hacen visibles los estudios que presentaron datos sociodemográficos más detallados. Se concluyó que existe la necesidad de ampliar la información divulgada para visibilizar a grupos socialmente minoritarios. Para eso, se presentan propuestas que tienen como objetivo contribuir a la visibilidad de dichos grupos, en consonancia con las reglamentaciones actuales del Consejo Federal de Psicología, y con el cruce de información relevante para la construcción de un saber interseccional en el área de la evaluación psicológica. (AU)

Antimitotic and toxicogenetic action of Stevia urticifolia aerial parts on proliferating vegetal and mammalian cells: in vitro and in vivo traditional and replacement methods.

Stevia urticifolia Thunb. is an underexploited herb possessing bioactive flavonoids, saponins, and terpenoids. The aim of this study was to examine the antiproliferative and toxicogenetic properties of the ethyl acetate extract from Stevia urticifolia aerial parts (EtAcSur) upon Artemia salina, erythrocytes, Allium cepa and sarcoma 180 cells and fibroblasts, as well as in vivo studies on mice to determine systemic, macroscopic, and behavioral alterations and bone marrow chromosomal damage. The assessment using A. salina larvae and mouse blood cells revealed LC50 and EC50 values of 68.9 and 113.6 µg/ml, respectively. Root growth and mitosis were inhibited by EtAcSur, and chromosomal aberrations were detected only at 100 µg/ml. EtAcSur exhibited potent concentration-dependent viability reduction of S180 and L-929 cells and antioxidant capacity employing ABTS• and DPPH•. No previous in vivo studies were performed before with the EtAcSur. Signals of acute toxicity were not observed at 300 mg/kg. Physiological and toxicological investigations at 25 and 50 mg/mg/day i.p. for 8 days did not markedly change body or organ relative weights, nor patterns of spontaneous locomotor and exploratory activities. In contrast, clastogenic effects on bone marrow were found at 50 mg/mg/day. EtAcSur was found to (1) produce toxicity in microcrustaceans, (2) capacity as free radical scavenger, (3) antimitotic, cytotoxic and clastogenic activties upon vegetal and mammalian cells, and (4) lethality on both tumor and normal murine cells indistinctly. In vivo damage systemic effects were not remarkable and clinical signals of toxicity were not observed, suggesting the significant pharmacological potential of S. urticifolia for the development of antineoplastic agents.Abbreviations: ABTS: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid); DMSO: dimethylsulfoxide; DPPH: 1,1-diphenyl-2-picrylhydrazyl; EC50: effective concentration 50%; EtAcSur: ethyl acetate extract from Stevia urticifolia aerial parts; Hb, hemoglobin; IC50: inhibitory concentration 50%; LC50,: lethal concentration 50%; MI: mitotic index; RBC, red blood cells; Trolox: 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid.

Investigating the influence of Food Safety Management Systems (FSMS) on microbial diversity of Canastra cheeses and their processing environments.

Canastra Cheese is one of the most commercialized artisanal cheeses in Brazil and intrinsic characteristics of its production, such as the use of raw milk and natural whey starter cultures as well short ripening time on wooden shelves, offer risk of contamination by a plethora of microorganisms. Here, we used 16 S rRNA gene amplicon sequencing approach to characterize the bacterial communities from Canastra cheese processing environments and final products, accessing cheesemaking facilities with distinct profiles of Food Safety Management Systems (FSMS), in order to estimate whether differences in microbial composition and diversity could also be observed between the two sampled groups of facilities. Our results revealed that the diversity of bacterial communities in the processing environments was much higher than that observed for cheeses, with greater discrepancy for facilities with inadequate FSMS. Additionally, in facilities with inadequate FSMS the bacterial communities from environments, especially hand surfaces and ripening wooden shelves, were similar to those during processing and finished cheese. These evidences highlight the importance of implementing and maintaining FSMS in the facilities, in order to assure quality and safety of Canastra cheese, but also the stability and economic viability of the Canastra cheese production chain.

The impact of packaging design on the perceived quality of honey by Brazilian consumers.

This study aimed to construct and validate a scale to evaluate the honey consumer perception. Furthermore, the impact of packaging design on honey's perceived quality and purchase intention was evaluated. Brazilian consumers (n = 343) answered the 21 self-descriptive statements of the scale using 7-point Likert scales. Furthermore, six different packages were presented, and the consumer perception (health, origin, safety, and taste) and purchase intention were evaluated using 5-point and 7-point Likert scales, respectively. Exploratory and confirmatory factor analyses and PLS path modeling were employed. The validated scale had 13 self-descriptive statements (indicators with factor loading higher than 0.4) and showed discriminant (heterotrait-monotrait ratio values < 0.85) and convergent validity (average variance extracted > 0.4) and adequate reliability (composite reliability > 0.70). The consumers associated honey with health properties and a safe product. Furthermore, they preferred honey purchased directly from producers due to its perceived quality (natural and pure). Honey packaged in glass jars with or without dipper was perceived as healthier, tastier, higher quality, and from trusted origin. Furthermore, glass jars were considered more practical and sustainable packages.

Spatiotemporal dynamics of the resistome and virulome of riverine microbiomes disturbed by a mining mud tsunami.

Aquatic ecosystems are highly vulnerable to anthropogenic activities. However, it remains unclear how the microbiome responds to press disturbance events in these ecosystems. We examined the impact of the world's largest mining disaster (Brazil, 2015) on sediment microbiomes in two disturbed rivers compared to an undisturbed river during 390 days post-disturbance. The diversity and structure of the virulome and microbiome, and of antibiotic and metal resistomes, consistently differed between the disturbed and undisturbed rivers, particularly at day 7 post-disturbance. 684 different ARGs were predicted, 38% were exclusive to the disturbed rivers. Critical antibiotic resistance genes (ARGs), e.g., mcr and ereA2, were significantly more common in the disturbed microbiomes. 401 different ARGs were associated with mobile genetic elements (MGEs), 95% occurred in the disturbed rivers. While plasmids were the most common MGEs with a broad spectrum of ARGs, spanning 16 antibiotic classes, integrative conjugative elements (ICEs) and integrons disseminated ARGs associated with aminoglycoside and tetracycline, and aminoglycoside and beta-lactam, respectively. A significant increase in the relative abundance of class 1 integrons, ICEs, and pathogens was identified at day 7 in the disturbed microbiomes, 72-, 14- and 3- fold higher, respectively, compared with the undisturbed river. Mobile ARGs associated with ESKAPEE group pathogens, while metal resistance genes and virulence factor genes in nonpathogenic hosts predominated in all microbiomes. Network analysis showed highly interconnected ARGs in the disturbed communities, including genes targeting antibiotics of last resort. Interactions between copper and beta-lactam/aminoglycoside/macrolide resistance genes, mostly mobile and critical, were also uncovered. We conclude that the mud tsunami resulted in resistome expansion, enrichment of pathogens, and increases in promiscuous and mobile ARGs. From a One Health perspective, mining companies need to move toward more environmentally friendly and socially responsible mining practices to reduce risks associated with pathogens and critical and mobile ARGs.

Microbial composition of a hydropower cooling water system reveals thermophilic bacteria with a possible role in primary biofilm formation.

Microfouling, ie biofilm formation on surfaces, can have an economic impact and requires costly maintenance in water-powered energy generation systems. In this study, the microbiota of a cooling system (filter and heat exchanger) in the Irapé hydroelectric power plant in Brazil was examined. The goal was to identify bacteria that could be targeted to more efficiently reduce biofilm formation. Two sampling campaigns were made corresponding to two well-defined seasons of the Brazilian Cerrado biome: the dry (campaign 1) and the wet (campaign 2). Microfouling communities varied considerably over time in samples obtained at different times after the last clearance of the heat exchanger. The thermophilic bacteria Meiothermus, Thermomonas and Symbiobacterium were exclusive and abundant in the microfouling of the heat exchanger in campaign 2, while methanotrophs and iron-reducing bacteria were abundant only in filter sediments. These findings could help to guide strategies for ecofriendly measures to reduce biofilm fouling in hydroelectric power plants, minimizing environmental and economic losses.

Comprehensive insights into arsenic- and iron-redox genes, their taxonomy and associated environmental drivers deciphered by a meta-analysis.

In nature, arsenic (As) and iron (Fe) biotransformation are interconnected, influencing local As mobility and toxicity. While As- or Fe-metabolizing microorganisms are widely documented, knowledge concerning their cycling genes, associated with geophysicochemical data and taxonomic distribution, remains scarce. We performed a meta-analysis to explore the distribution and environmental importance of As- and Fe-redox genes (AsRGs and FeRGs) and predict their significant correlations and hosts. The most abundant and ubiquitous AsRGs and FeRGs were arsC and ccoN, respectively. The ccoN gene had the highest frequency at pH ≥ 9.1, in which dissolved Fe(II) is scarce, possibly contributing to enhanced host survival. Fe(III) oxidation genes iro and ccoN appear to be associated with As(V) detoxification in mesophilic environments. No correlation was observed between Fe(III) reduction gene omcB and arsenate reductase genes. Cytochromes with putative roles in Fe-redox reactions were identified (including yceJ and fbcH) and were significantly correlated with As(V) reduction genes under diverse geophysicochemical conditions. The taxonomies of AsRGs and FeRGs-carrying contigs revealed great diversity, among which various, such as Chlamydea (arsC) and Firmicutes (omcB), were previously undescribed. Nearly all (98.9%) of the AsRGs and FeRGs were not carried by any plasmid sequences. This meta-analysis expands our understanding of the global environmental, taxonomic and functional microbiome involved in As- and Fe-redox transformations. Moreover, these findings should help guide studies on putative in vivo functional roles of cytochromes in Fe-redox pathways.

Exploring antibiotic resistance in environmental integron-cassettes through intI-attC amplicons deep sequencing.

INTRODUCTION: Freshwater ecosystems provide propitious conditions for the acquisition and spread of antibiotic resistance genes (ARGs), and integrons play an important role in this process. MATERIAL AND METHODS: In the present study, the diversity of putative environmental integron-cassettes, as well as their potential bacterial hosts in the Velhas River (Brazil), was explored through intI-attC and 16S rRNA amplicons deep sequencing. RESULTS AND DISCUSSION: ORFs related to different biological processes were observed, from DNA integration to oxidation-reduction. ARGs-cassettes were mainly associated with class 1 mobile integrons carried by pathogenic Gammaproteobacteria, and possibly sedentary chromosomal integrons hosted by Proteobacteria and Actinobacteria. Two putative novel ARG-cassettes homologs to fosB3 and novA were detected. Regarding 16SrRNA gene analysis, taxonomic and functional profiles unveiled Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria as dominant phyla. Betaproteobacteria, Alphaproteobacteria, and Actinobacteria classes were the main contributors for KEGG orthologs associated with resistance. CONCLUSIONS: Overall, these results provide new information about environmental integrons as a source of resistance determinants outside clinical settings and the bacterial community in the Velhas River.

Preparation of glass-ionomer cement containing ethanolic Brazilian pepper extract (Schinus terebinthifolius Raddi) fruits: chemical and biological assays.

Plants may contain beneficial or potentially dangerous substances to humans. This study aimed to prepare and evaluate a new drug delivery system based on a glass-ionomer-Brazilian pepper extract composite, to check for its activity against pathogenic microorganisms of the oral cavity, along with its in vitro biocompatibility. The ethanolic Brazilian pepper extract (BPE), the glass-ionomer cement (GIC) and the composite GIC-BPE were characterized by scanning electron microscopy, attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), and thermal analysis. The BPE compounds were identified by UPLC-QTOF-MS/MS. The release profile of flavonoids and the mechanical properties of the GIC-BPE composite were assessed. The flavonoids were released through a linear mechanism governing the diffusion for the first 48 h, as evidenced by the Mt/M∞ relatively to [Formula: see text], at a diffusion coefficient of 1.406 × 10-6 cm2 s-1. The ATR-FTIR analysis indicated that a chemical bond between the GIC and BPE components may have occurred, but the compressive strength of GIC-BPE does not differ significantly from that of this glass-ionomer. The GIC-BPE sample revealed an ample bacterial activity at non-cytotoxic concentrations for the human fibroblast MRC-5 cells. These results suggest that the prepared composite may represent an alternative agent for endodontic treatment.

Impactos da Covid-19 na mobilidade, na acessibilidade e no trabalho do psicólogo do trânsito / Impacts of Covid-19 at mobility, accessibility and work of the traffic psychologist / Impactos del Covid-19 en la movilidad, la accesibilidad y el trabajo del psicólogo de tránsito

Resumo Neste artigo, discutem-se os impactos da Covid-19 na mobilidade, na acessibilidade de pessoas com deficiência e no trabalho do psicólogo do trânsito. Em relação à mobilidade, são apontados dados sobre a demanda de viagem nos transportes urbanos e suas repercussões sociais e econômicas, como o desemprego e o incremento do transporte clandestino com o fechamento de empresas de transporte. São discutidos também o uso de transportes sustentáveis como alternativa e o investimento em comunidades locais autossuficientes para diminuir os deslocamentos. Em relação aos impactos na acessibilidade, comentaremos sobre os avanços na legislação e a mudança de cultura visando à concretização das práticas de inclusão. Também exemplificamos como pessoas com deficiência vivenciam riscos adicionais de contrair a Covid-19 nos deslocamentos. Quanto aos impactos no trabalho do psicólogo do trânsito, analisam-se a suspensão das atividades presenciais e seu retorno e como os psicólogos continuaram desempenhando seu trabalho, desde a perícia psicológica para a habilitação e docência, passando pela gestão de projetos e políticas públicas (e.g., educação para o trânsito) e de associações profissionais. As expectativas para o pós-pandemia e os seus aprendizados também são discutidas. Conclui-se que a pandemia da Covid-19 potencializou o contexto, já presente antes da pandemia, de desigualdade estrutural nas cidades, na mobilidade e na acessibilidade, bem como acentuou a necessidade de adaptação dos processos de trabalho da psicologia. Vislumbram-se mudanças e avanços possíveis, priorizando a diversidade da coletividade na busca de soluções que atendam aos mais diversos grupos de pessoas.

Abstract In this article, there is a discussion about the impacts of Covid-19 at the mobility, accessibility of people with disabilities and the work of the traffic psychologist. Concerning to mobility, the data of requests about using urban transportation and social and economic repercussion are emphasize, such as unemployment and illegal transportation increase, resulting in transport companies closure. The employ of the sustainable transportation as an alternative and investment on sustainable local communities to reduce travels are also discussed. Regarding to accessibility impacts, it will be comment about the law advances and the culture changes, with the aim of concretize inclusion practices. It also exemplifies how disable people live with additional risks of contracting Covid-19 when they travel. As for the impacts of traffic psychologist work, the suspension of face-to-face and their return are analyzed, and how those professionals will do their jobs, from psychologist expertise to qualification and teaching, going through projects and public policies management, just as traffic education, and professional association management. Expectations for the post-pandemic and its learning are also discussed. It is concluded that the Covid-19 pandemic has enhanced the context of structural inequality in cities, mobility and accessibility already present before the pandemic, as well as emphasizing the need to adapt psychology's work processes. Changes and possible advances are envisaged, prioritizing the community diversity at the pursuit of solutions that can assist the most diverse group of people.

Resumen En este artículo, discutimos los impactos del Covid-19 en la movilidad, la accesibilidad para personas con discapacidad y el trabajo del psicólogo de tránsito. En relación a la movilidad, se señalan datos sobre la demanda de viajes en el transporte urbano y sus repercusiones sociales y económicas, como el desempleo y el incremento del transporte ilegal con el cierre de empresas de transporte. También se discute el uso del transporte sostenible como alternativa y la inversión en comunidades locales autosuficientes para reducir los desplazamientos. En cuanto a los impactos en la accesibilidad, comentaremos los avances en la legislación y el cambio de cultura con miras a implementar prácticas de inclusión. También se ejemplifica cómo las personas con discapacidades experimentan riesgos adicionales de contraer el Covid-19 en los desplazamientos. En cuanto a los impactos en el trabajo del psicólogo de tránsito, se analiza la suspensión de las actividades presenciales y su retorno, y cómo los psicólogos continuaron realizando su labor, desde la pericia psicológica hasta la capacitación y docencia, pasando por la gestión de proyectos y políticas públicas (como la educación vial) y la gestión de asociaciones profesionales. Además, se analizan las expectativas para la pospandemia y su aprendizaje. Se concluye que la pandemia de Covid-19 ha potenciado el contexto de desigualdad estructural en las ciudades, la movilidad y la accesibilidad, ya presentes antes de la pandemia, además de enfatizar la necesidad de adecuar los procesos de trabajo de la psicología. Se perciben posibles cambios y avances priorizando la diversidad de la colectividad en la búsqueda de soluciones que atiendan a los más diversos grupos de personas.

Characterizing a riverine microbiome impacted by extreme disturbance caused by a mining sludge tsunami.

Studies on disturbance events in riverine systems caused by environmental disasters and their effects on microbial diversity are scarce. Here, we evaluated the impact of the collapse of an iron ore dam holding approximately 50 million cubic meters of waste on both water and sediment microbiomes by deeply sequencing the 16S rRNA gene. Samples were taken from two impacted rivers and one reference river 7, 30 and 150 days postdisturbance. The impacted community structure changed greatly over spatiotemporal scales, being less diverse and more uneven, particularly on day 7 for the do Carmo River (the closest to the dam). However, the reference community structure remained similar between sampling events. Moreover, the impacted sediments were positively correlated with metals. The taxa abundance varied greatly over spatiotemporal scales, allowing for the identification of several potential bioindicators, e.g., Comamonadaceae, Novosphingobium, Sediminibacterium and Bacteriovorax. Our results showed that the impacted communities consisted mostly of Fe(II) oxidizers and Fe(III) reducers, aromatic compound degraders and predator bacteria. Network analysis showed a highly interconnected microbiome whose interactions switched from positive to negative or vice versa between the impacted and reference communities. This work revealed potential molecular signatures associated with the rivers heavily impacted by metals that might be useful sentinels for predicting riverine health.

"Pretitude" e o Afroperspectivismo em Psicoterapia: Desafios para a Abordagem Gestáltica / "Pretitude" and Afroperspectivism in Psychotherapy: Challenges for the Gestalt Approach / "Pretitude" y Afroperspectivismo en Psicoterapia: Desafíos para el Enfoque de la Gestalt

Apresentamos a intervenção do projeto de extensão "Gestalt-Terapia, escuta e acolhimento psicológico de grupos", realizado desde 2018 por alunos extensionistas negros do Curso de Psicologia da Universidade Federal do Espírito Santo utilizando a abordagem Gestáltica. O objetivo é desenvolver a prática do acolhimento psicológico de grupos, para homens e mulheres negras, principalmente universitários. Para tanto, foram realizados 07 encontros no ano de 2018 (04 participantes) e 15 encontros no ano de 2019 (07 participantes), sendo 04 mulheres e 07 homens, com idades entre 19 e 45 anos. Os encontros aconteceram no Núcleo de Psicologia Aplicada em horário noturno. Os principais recursos terapêuticos são provenientes da expressão verbal e corporal, entretanto, músicas, vivências e atividades de colagens foram agregadas. Os resultados parciais indicaram que os encontros grupais proporcionaram cuidado e reconhecimento de si, do mundo e do outro, e melhora da autoestima na população assistida. Os participantes reconheceram muitos processos que interrompem as trocas afetivas, como resultado do racismo estrutural que a população negra brasileira vivencia. A consciência corporal e afetiva possibilitou uma reaproximação dos afetos positivos entre pessoas negras, de modo a reafirmar as características positivas dessa população. (AU)

We present the intervention of the extension project "Gestalt-Therapy, listening and psychological reception of groups", conducted since 2018 by black extension students of the Psychology Course of the Federal University of Espírito Santo using the Gestaltic approach. The objective is to develop the practice of psychological group reception for black men and women, mainly university students. To this end, 07 meetings were held in 2018 (04 participants) and 15 meetings in 2019 (07 participants), 04 women and 07 men, aged 19 to 45 years old. The meetings took place at the Center for Applied Psychology at night time. The main therapeutic resources came from verbal and body expression, however, songs, experiences and collage activities were added. The partial results indicated that the group meetings provided care and recognition of self, of the world and the other, it also improved self-esteem in the assisted population. The participants recognized many processes that interrupt affective exchanges as a result of the structural racism that the black Brazilian population experiences. Body and affective awareness made it possible to re-approach positive affections among black people in order to reaffirm the positive characteristics of this population. (AU)

Presentamos la intervención del proyecto de extensión "Terapia Gestalt, escucha y acogimiento psicológico de grupos", realizado desde 2018 por estudiantes negros extensionistas del Curso de Psicología de la Universidad Federal do Espírito Santo utilizando el enfoque Gestáltico. El objetivo es desarrollar una práctica de acogimiento psicológico de grupos, para hombres y mujeres negros, principalmente estudiantes universitarios. Para esto, fue realizado 07 encuentros en el año de 2018 (04 participantes) y 15 encuentros en el año de 2019 (07 participantes), siendo 04 mujeres y 07 hombres, con edades comprendidas entre 19 y 45 años. Los encuentros ocurrieron en el Núcleo de Psicología Aplicada en horario nocturno. Los principales recursos terapéuticos provenían de la expresión verbal y corporal, sin embargo, se agregaron la música, las experiencias y actividades de collage. Los resultados parciales indicaron que los encuentros grupales proporcionaron cuidado y reconocimiento de sí mismos, del mundo y del otro, y mejoraron la autoestima de la población asistida. Los participantes reconocieron muchos procesos que interrumpieron, destacándose los intercambios afectivos como resultado de un racismo estructural que la populación negra brasileña experimenta. La consciencia corporal y afectiva posibilitó una reaproximación de los afectos positivos entre personas negras, a modo de reafirmar las características positivas de esta populación. (AU)

Exploring the resistome, virulome and microbiome of drinking water in environmental and clinical settings.

Aquatic ecosystems harbor a vast pool of antibiotic resistance genes (ARGs), which can suffer mutation, recombination and selection events. Here, we explored the diversity of ARGs, virulence factors and the bacterial community composition in water samples before (surface raw water, RW) and after (disinfected water, DW) drinking water conventional treatment, as well as in tap water (TW) and ultrafiltration membranes (UM, recovered from hemodialysis equipment) through metagenomics. A total of 852 different ARGs were identified, 21.8% of them only in RW, which might reflect the impact of human activities on the river at the sampling point. Although a similar resistance profile has been observed between the samples, significant differences in the frequency of clinically relevant antibiotic classes (penam and peptide) were identified. Resistance determinants to last resort antibiotics, including sequences related to mcr, optrA and poxtA and clinically relevant beta-lactamase genes (i.e. blaKPC, blaGES, blaIMP, blaVIM, blaSPM and blaNDM) were detected. 830 coding sequences (CDSs - related to 217 different ARGs) were embedded in contigs associated with mobile genetic elements, specially plasmids, of which 68% in RW, DW and TW, suggesting the importance of water environments in resistance dissemination. Shifts in bacterial pathogens genera were observed, such as a significant increase in Mycobacterium after treatment and distribution. In UM, the potentially pathogenic genus Halomonas predominated. Its draft genome was closely related to H. stevensii, hosting mainly multidrug efflux pumps. These results broaden our understanding of the global ARGs diversity and stress the importance of tracking the ever-expanding environmental resistome.