Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 4 de 4
Filter
Add more filters










Publication year range
1.
Am J Primatol ; 70(5): 423-31, 2008 May.
Article in English | MEDLINE | ID: mdl-18080315

ABSTRACT

Cytochrome b DNA sequence data (ca. 1,140 bp) of 66 Alouatta belzebul from the Amazonian and the Atlantic Forests of Brazil were used for phylogenetic reconstructions and population studies. Our sample consisted of 60 specimens from the Amazonian Forest (captured in 1984 and 1998 in Pará-PA state) and six specimens from the Atlantic Forest (Paraíba-PB state). We found 32 haplotypes, 23 in PA-1984 (with 12 present in more than one individual), 11 in PA-1998 (with two present in more than one individual), and a single haplotype in the PB sample. Animals from PA-1984 and PA-1998 shared three haplotypes while animals from Pará and Paraíba did not share any haplotype. We found 57 variable sites, consisting of 53 transitions and four transversions, with most replacements occurring at third codon position (77.19%) and less frequently at first and second positions (10.53 and 12.28%, respectively). Genetic distance between all haplotypes varied between 0 and 1.2%. Nucleotide diversity estimates between PA-1984 haplotypes and PA-1998 haplotypes were the same (π=0.01), and haplotype diversity estimates were very similar (h=0.96 and 0.93 for PA-1984 and PA-1998, respectively). Maximum parsimony, median-joining, split decomposition, and TCS showed that PA and PB haplotypes had not drastically diverged and that subsequent radiation within these regions was not apparent. No temporal structure was found between PA-1984 and PA-1998. The sum of square deviation estimate for PA-1984 equaled 0.01 (P=0.23), in agreement with a hypothetical model of sudden expansion contrary to PA-1998 whose sum of square deviation estimate (0.40; P=0.04) was not compatible with this model, although the small sample size of PA-1998 as well as the smaller area of capture could have also accounted for this result. Fu's F(s) and R(2) statistical neutrality tests corroborated these propositions. Lack of drastic differentiation was attributable to the once existing connection between the Atlantic and the Amazonian forests at a non-distant past.


Subject(s)
Alouatta/genetics , Cytochromes b/genetics , Animals , Base Sequence , Brazil , Cytochromes b/chemistry , DNA, Mitochondrial/chemistry , DNA, Mitochondrial/genetics , Genetic Variation , Haplotypes , Molecular Sequence Data , Phylogeny , Polymerase Chain Reaction/veterinary , Trees
2.
Cytogenet Genome Res ; 108(1-3): 106-11, 2005.
Article in English | MEDLINE | ID: mdl-15545722

ABSTRACT

We carried out a phylogenetic and population study in Alouatta caraya and Alouatta belzebul based on cytochrome b DNA sequence data. Maximum Parsimony and Median-Joining analyses grouped A. caraya from different localities showing a population structure in accordance with geographic distribution. The relation between A. caraya haplotypes could be explained with respect to the species range in the Cerrado, one of the most ancient morphoclimatic domains of South America, and the Chaco. Conversely, A. belzebul from the Amazonas and Atlantic forests grouped in a paraphyletic arrangement without an evident geographic pattern. Recent geologic events resulting in the separation of A. belzebul might explain why these geographically distant groups shared similar haplotypes and why ancestral polymorphisms might have been maintained in this species. Time of divergence estimates indicated that the splitting of the Alouatta lineage leading to A. caraya occurred some 4.58 MYA while the lineage leading to A. belzebul emerged 4.14 MYA.


Subject(s)
Alouatta/genetics , Cytochromes b/genetics , Genetics, Population/methods , Polymorphism, Genetic/genetics , Animals , Evolution, Molecular , Molecular Sequence Data , Phylogeny , Species Specificity
3.
Am J Primatol ; 61(3): 123-33, 2003 Nov.
Article in English | MEDLINE | ID: mdl-14610730

ABSTRACT

Morphologic, karyotypic, and molecular analyses were carried out in 25 specimens of a distinct morph of Chiropotes (henceforth termed Chiropotes sp.) obtained from a number of localities in the Brazilian Amazon. Pelage coloration clearly distinguishes the collected specimens and all other known species of this genus. A distinct karyotype was described for Chiropotes sp. It differs from C. satanas chiropotes by two pericentric inversions, and from C. satanas utahicki by three, which suggests that these taxa are reproductively isolated. Morphometric analyses did not show significant differentiation between these Chiropotes taxa. Molecular analyses confirmed the monophyly of the subfamily Pitheciinae and genera Chiropotes, Cacajao, and Pithecia (the latter appearing as the most basal lineage of the pithecine clade). The genetic distances between C. s. utahicki and Chiropotes sp. from Rio Negro were greater than those between three recognized species of Pithecia, but smaller than those between Cacajao calvus and Cacajao melanocephalus. The most appropriate name for Chiropotes sp. from Rio Negro is C. israelita. This species, C. s. chiropotes, and C. s. utahicki are allopatric. Pelage coloration, karyotype, and molecular analysis strongly indicate that C. chiropotes, C. utahicki, and Chiropotes israelita deserve species status.


Subject(s)
Body Weights and Measures , Cebidae/genetics , Genetic Variation , Hair Color/genetics , Animals , Base Sequence , Brazil , Cluster Analysis , Evolution, Molecular , Geography , Karyotyping , Molecular Sequence Data , Phylogeny , Sequence Analysis, DNA , Species Specificity
4.
J. bras. nefrol ; 6(4): 115-8, 1984.
Article in Portuguese | LILACS | ID: lil-23001

ABSTRACT

Uma avaliacao bacteriologica sobre infeccao urinaria realizada em individuos pertencentes a tres faixas etarias distintas - criancas, adultos e idosos - mostrou que o principal agente etiologico dessas infeccoes e a Escherichia coli (58,5%), seguida de Proteus mirabilis (20,6%),Enterobacter (E.aglomerans, E. cloacae, E. aerogenes) (9%), Staphylococcus aureus (6%) e outros em frequencias mais baixas. O antibiotico de maior espectro de acao foi amicacina. Para as enterobacterias os antibioticos de maior atividade (70%) foram acido nalidixico e tobramicina. As amostras de S. aureus foram sensiveis (80%) a oxacilina, cefalotina, neomicina,estreptomicina e amoxacilina. A E. coli foi sensivel, a nivel de 70% das amostras, aos seguintes antibioticos: nitrofurantoina,acido pipemidico, gentamicina, amicacina, tobramicina e acido nalidixico


Subject(s)
Infant , Child, Preschool , Child , Adolescent , Adult , Middle Aged , Humans , Male , Female , Bacterial Infections , Urinary Tract Infections , Age Factors , Anti-Bacterial Agents , Drug Resistance, Microbial
SELECTION OF CITATIONS
SEARCH DETAIL
...