Neural respiratory drive during maximal voluntary ventilation in individuals with hypertension: A case-control study.

Neural respiratory drive (NRD) is measured using a non-invasive recording of respiratory electromyographic signal. The parasternal intercostal muscle can assess the imbalance between the load and capacity of respiratory muscles and presents a similar pattern to diaphragmatic activity. We aimed to analyze the neural respiratory drive in seventeen individuals with hypertension during quite breathing and maximal voluntary ventilation (MVV) (103.9 ± 5.89 vs. 122.6 ± 5 l/min) in comparison with seventeen healthy subjects (46.5 ± 2.5 vs. 46.4 ± 2.4 years), respectively. The study protocol was composed of quite breathing during five minutes, maximum inspiratory pressure followed by maximal ventilatory ventilation (MVV) was recorded once for 15 seconds. Anthropometric measurements were collected, weight, height, waist, hip, and calf circumferences, waist-to-hip ratio (WHR), waist-to-height ratio (WHtR), BMI, and conicity index (CI). Differences between groups were analyzed using the unpaired t-test or Mann-Whitney test to determine the difference between groups and moments. A significance level of 5% (p<0,05) was adopted for all statistical analyses. The group of individuals with hypertension presented higher values when compared to the healthy group for neural respiratory drive (EMGpara% 17.9±1.3 vs. 13.1±0.8, p = 0.0006) and neural respiratory drive index (NRDi (AU) 320±25 vs. 205.7±15,p = 0.0004) during quiet breathing and maximal ventilatory ventilation (EMGpara% 29.3±2.7 vs. 18.3±0.8, p = 0.000, NRDi (AU) 3140±259.4 vs. 1886±73.1,p<0.0001), respectively. In conclusion, individuals with hypertension presented higher NRD during quiet breathing and maximal ventilatory ventilation when compared to healthy individuals.

Repetitive DNAs and chromosome evolution in Megaleporinus obtusidens and M. reinhardti (Characiformes: Anostomidae).

The high dynamism of repetitive DNAs is a major driver of chromosome evolution. In particular, the accumulation of repetitive DNA sequences has been reported as part of the differentiation of sex-specific chromosomes. In turn, the fish species of the genus Megaleporinus are a monophyletic clade in which the presence of differentiated ZZ/ZW sex chromosomes represents a synapomorphic condition, thus serving as a suitable model to evaluate the dynamic evolution of repetitive DNA classes. Therefore, transposable elements (TEs) and in tandem repeats were isolated and located on chromosomes of Megaleporinus obtusidens and M. reinhardti to infer their role in chromosome differentiation with emphasis on sex chromosome systems. Despite the conserved karyotype features of both species, the location of repetitive sequences - Rex 1, Rex 3, (TTAGGG)n, (GATA)n, (GA)n, (CA)n, and (A)n - varied both intra and interspecifically, being mainly accumulated in Z and W chromosomes. The physical mapping of repetitive sequences confirmed the remarkable dynamics of repetitive DNA classes on sex chromosomes that might have promoted chromosome diversification and reproductive isolation in Megaleporinus species.

Evaluation of mobile applications focused on the care of patients with anxiety disorders: A systematic review in app stores in Brazil.

OBJECTIVE: To identify and evaluate the quality of mobile apps available in Brazil focused on the care of patients with anxiety disorders. METHODS: A comprehensive search was conducted until October 2021 on Play Store (Android) and Apple Store (iOS) in Brazil, using the terms "anxiety," "phobia," "panic attack," and "social phobia." Two independent authors identified the apps and performed data extraction and quality assessment using the Mobile App Rating Scale (MARS). Pearson's correlation was used to analyze the relationship between user star rating and the quality defined by the MARS instrument. RESULTS: A total of 3,278 potential apps were identified, of which 71 fully met the eligibility criteria. Most apps were made available on the Play Store (91.74%), in English (69.01%), and updated in the last two years (90.14%). Approximately half of the apps (50.70%) did not inform the developer's country and most of them did not report the user star rating (70.42%). The target population was indicated as free by most apps (85.92%), with generalized anxiety disorder being the most addressed disorder (74.65%), followed by panic disorder (33.80%). The three main purposes of the apps were education (83.10%), self-assessment (38.03%), and meditation/breathing (32.39%). Only 31 apps (43.66%) had acceptable quality (above 3.0) and the average total MARS quality score of 2.93 (2.20 to 3.90), with the functionality section receiving the highest score (3.90) and the lowest scoring sections being engagement (2.16). The apps that were rated stars by users (29.58%) showed a negative Pearson correlation (ρ = -0.100), evidencing a difference in the user's evaluation and that performed using the MARS instrument. CONCLUSION: Gaps in the quality of apps focused on the care of patients with anxiety disorders were evidenced since most were classified as having low quality through the MARS instrument. Thus, users are recommended to use these apps with caution.

Development and validation of LC-MS/MS methods for the simultaneous quantification of sofosbuvir and its major metabolite (GS-331007) in blood plasma and cerebrospinal and seminal fluid: Application to a pilot clinical trial with a focus on Zika.

Zika still poses a threat to global health owing to its association with serious neurological conditions and the absence of a vaccine and treatment. Sofosbuvir, an anti-hepatitis C drug, has shown anti-Zika effects in animal and cell models. Thus, this study aimed to develop and validate novel LC-MS/MS methods for the quantification of sofosbuvir and its major metabolite (GS-331007) in human plasma and cerebrospinal (CSF) and seminal fluid (SF), and apply the methods to a pilot clinical trial. The samples were prepared by liquid-liquid extraction and separated using isocratic mode on Gemini C18 columns. Analytical detection was performed using a triple quadrupole mass spectrometer equipped with an electrospray ionization source. The validated ranges for sofosbuvir were 0.5-2,000 ng/mL (plasma) and 0.5-100 ng/mL (CSF and SF), while for the metabolite they were 2.0-2,000 ng/mL (plasma), 5.0-200 ng/mL (CSF) and 10-1,500 ng/mL (SF). The intra-day and inter-day accuracies (90.8-113.8%) and precisions (1.4-14.8%) were within the acceptance range. The developed methods fulfilled all validation parameters concerning selectivity, matrix effect, carryover, linearity, dilution integrity, precision, accuracy and stability, confirming the suitability of the method for the analysis of clinical samples.

Linguistic profile of children with language and speech disorders and family participation in the therapeutic process: an integrative literature review / Perfil linguístico de crianças com transtornos de linguagem e fala e participação da família no processo terapêutico: revisão integrativa de literatura

ABSTRACT Purpose: to investigate the linguistic profile (linguistic symptoms and risk factors) of children with communication, language, and speech disorders and verify their family's participation in the therapeutic process. Methods: an integrative literature review including studies on linguistic symptoms of children with developmental language disorder (DLD), autism spectrum disorder (ASD), and phonological disorders (PD), published in Portuguese in the last 10 years (2012 to 2022) in the PubMed, SciELO, and VHL databases. Literature Review: 24 studies were included, according to the eligibility criteria, the children having different linguistic symptoms. In PD, there were more phonological processes, slower speech, and difficulties in auditory perception and morphosyntactic and semantic development. In DLD, there were changes in nominal morphology, morphosyntactic comprehension, greater use of intransitive verbs, omission of verb objects, and difficulties in solving conflicts. In ASD, there were difficulties in social interaction, imitation, prosodic changes, and decreased responsiveness. The main risk factor was prematurity, and the family was considered crucial as a support network in the therapeutic process. The family's attitudes were facilitators or barriers to the patients' improvement, depending on how they responded to the therapists' instructions. Conclusion: the linguistic profile depends on the various language/speech disorders. Each child's singularities must be considered, and the family's participation is crucial to the therapeutic process.

RESUMO Objetivo: investigar o perfil linguístico (sintomas linguísticos e fatores de risco) de crianças com transtornos de comunicação, linguagem e fala e verificar a participação da família no processo terapêutico. Métodos: foi realizada uma revisão integrativa da literatura. Foram incluídos estudos sobre sintomas linguísticos de crianças com Transtorno do Desenvolvimento da Linguagem (TDL), TEA (Transtorno do Espectro Autista) e Transtornos Fonológicos (TF), publicados nos últimos 10 anos (2012 a 2022) em língua portuguesa nas bases de dados: PubMed, Scielo e BVS. Revisão da Literatura: aplicando os critérios de elegibilidade, foram incluídos 24 estudos. As crianças apresentaram diferentes sintomas linguísticos. No TF verificaram-se quantidade maior de processos fonológicos, menor velocidade de fala, dificuldades na percepção auditiva e no desenvolvimento morfossintático e semântico. No TDL verificaram-se alterações na morfologia nominal, na compreensão morfossintática, maior utilização de verbos intransitivos, omissões de complementos verbais e dificuldade em resolução de conflitos. No TEA observaram-se dificuldades na interação social, imitação, alterações prosódicas e diminuição na responsividade. O principal fator de risco foi a prematuridade e a família foi considerada crucial como rede de apoio no processo terapêutico. As atitudes da família foram facilitadoras ou barreiras para a melhora do paciente, dependendo da forma como as famílias correspondiam às orientações terapêuticas. Conclusão: o perfil linguístico depende dos diferentes transtornos de linguagem/fala, as singularidades de cada criança devem ser consideradas e a participação da família é crucial no processo terapêutico.

Cesarean Delivery and Risk of Excess Weight Among Brazilian Preschool Children.

OBJECTIVE: To study the relationship between cesarean Delivery (CD) and overweight in preschool children, considering the presence of birth aspects and demographic characteristics that have been associated with the risk of excess weight. METHODS: This retrospective cross-sectional analysis used representative data of children from 2 to 6 years of age at municipal daycare centers in Taubaté, São Paulo, Brazil. The sample included 752 preschoolers. Weight and height were collected in daycare centers, and the nutritional status classification was performed using z scores of Body Mass Index (zBMI). Data analysis was performed in the bivariate and multivariate manner, the latter with the inclusion of another possible risk factor. Adjustments were made for daycare centers time, sex, birth length and weight and maternal schooling. RESULTS: The mean age of preschool children was 4.7 years, and 53.8% were boys. The prevalence of overweight children was 21.7%. The median zBMI of the children born by CD was higher than that of vaginal Delivery (p = 0.0194). The prevalence of overweight showed association with CD (p = 0.0196; PR = 1.397 with 95% CI of 1065-1831). Logistic binary multivariate regression analysis confirmed the association, even in the presence of other possible risk factors (CR: 1.580, 95% CI: 1.072-2.330). CONCLUSION FOR PRACTICE: Birth by CD is one of the factors that are associated with the early development of overweight in preschoolers.

Origin of the Giant Enhanced Raman Scattering by Sulfur Chains Encapsulated inside Single-Wall Carbon Nanotubes.

In this work, we explain the origin and the mechanism responsible for the strong enhancement of the Raman signal of sulfur chains encapsulated by single-wall carbon nanotubes by running resonance Raman measurements in a wide range of excitation energies for two nanotube samples with different diameter distributions. The Raman signal associated with the vibrational modes of the sulfur chain is observed when it is confined by small-diameter metallic nanotubes. Moreover, a strong enhancement of the Raman signal is observed for excitation energies corresponding to the formation of excited nanotube-chain-hybrid electronic states. Our hypothesis was further tested by high pressure Raman measurements and confirmed by density functional theory calculations of the electronic density of states of hybrid systems formed by sulfur chains encapsulated by different types of single-wall carbon nanotubes.

Secure and Sustainable Sourcing of Plant Tissues for the Exhaustive Exploration of Their Chemodiversity.

The main challenge of plant chemical diversity exploration is how to develop tools to study exhaustively plant tissues. Their sustainable sourcing is a limitation as bioguided strategies and dereplication need quite large amounts of plant material. We examine if alternative solutions could overcome these difficulties by obtaining a secure, sustainable, and scalable source of tissues able to biosynthesize an array of metabolites. As this approach would be as independent of the botanical origin as possible, we chose eight plant species from different families. We applied a four steps culture establishment procedure, monitoring targeted compounds through mass spectrometry-based analytical methods. We also characterized the capacities of leaf explants in culture to produce diverse secondary metabolites. In vitro cultures were successfully established for six species with leaf explants still producing a diversity of compounds after the culture establishment procedure. Furthermore, explants from leaves of axenic plantlets were also analyzed. The detection of marker compounds was confirmed after six days in culture for all tested species. Our results show that the first stage of this approach aiming at easing exploration of plant chemodiversity was completed, and leaf tissues could offer an interesting alternative providing a constant source of natural compounds.

Karyotypic Diversification in Two Megaleporinus Species (Characiformes, Anostomidae) Inferred from In Situ Localization of Repetitive DNA Sequences.

Anostomidae species have conserved diploid numbers (2n = 54), although comparative cytogenetic studies have demonstrated chromosomal rearrangements occurrence among them, especially in repetitive DNA rich regions. The location and distribution of ribosomal DNA (rDNA) and small nuclear RNAs (snRNAs) multigene families are highly dynamic in the genomes of several organisms. In this study, we in situ located the rDNA and snRNA sites in two populations of Megaleporinus obtusidens and a sample of Megaleporinus reinhardti to infer their chromosomal changes in the evolutionary lineages. Both species of Megaleporinus shared 2n = 54 chromosomes with the presence of ZZ/ZW sex chromosome system, but they diverged in relationship to the location of 5S and 45S rDNAs as well as the distribution of snRNAs sites. The characterization of the analyzed sequences revealed the presence of complete rDNA and snRNAs sequences as well as snRNAs containing transposable elements (TEs) and microsatellite repeats. After chromosomal mapping, the sequences encompassing TEs proved to be dispersed through autosomes and accumulated on sex chromosomes. The data demonstrate that intra- and interspecific chromosomal changes occurred involving the multigene family's sites in Megaleporinus karyotypes. Furthermore, we detected TE-like sequences in the differentiation of sex chromosome systems in M. obtusidens and M. reinhardti.

Designing a cocktail containing redox enzymes to improve hemicellulosic hydrolysate fermentability by microorganisms.

Bioproducts production using monomeric sugars derived from lignocellulosic biomass presents several challenges, such as to require a physicochemical pretreatment to improve its conversion yields. Hydrothermal lignocellulose pretreatment has several advantages and results in solid and liquid streams. The former is called hemicellulosic hydrolysate (HH), which contains inhibitory phenolic compounds and sugar degradation products that hinder microbial fermentation products from pentose sugars. Here, we developed and applied a novel enzyme process to detoxify HH. Initially, the design of experiments with different redox activities enzymes was carried out. The enzyme mixture containing the peroxidase (from Armoracia rusticana) together with superoxide dismutase (from Coptotermes gestroi) are the most effective to detoxify HH derived from sugarcane bagasse. Butanol fermentation by the bacteria Clostridium saccharoperbutylacetonicum and ethanol production by the yeast Scheffersomyces stipitis increased by 24.0× and 2.4×, respectively, relative to the untreated hemicellulosic hydrolysates. Detoxified HH was analyzed by chromatographic and spectrometric methods elucidating the mechanisms of phenolic compound modifications by enzymatic treatment. The enzyme mixture degraded and reduced the hydroxyphenyl- and feruloyl-derived units and polymerized the lignin fragments. This strategy uses biocatalysts under environmentally friendly conditions and could be applied in the fuel, food, and chemical industries.

Evaluation of growth and nutritional condition of children in Public Schools in Florianópolis, Santa Catarina, Brazil / Avaliação do crescimento e do estado nutricional de crianças em idade escolar em Escolas Públicas de Florianópolis, Santa Catarina, Brasil

INTRODUCTION: Obesity is becoming more and more frequent in children, which can result in health problems both in childhood and in adulthood. Considering that, at school age, eating habits may be influenced by the context in which the child is inserted, mainly, lifestyle, inside and outside the school environment, it is important to evaluate the growth achieved in height, as well as their nutritional status, since this can be an indication of future and possible nutritional disorders, allowing to direct resources and effective public policies in this age groupOBJECTIVE: Evaluate the growth in stature and nutritional condition of schoolchildren by gender and age in Florianopolis, Santa Catarina, Brazil, in the first semester of the 2012 school yearMETHODS: A cross-sectional study was conducted with 595 children of six to ten years of age, through the marketing of Public Municipal Elementary Schools, in the city of Florianópolis, State of Santa Catarina, Brazil. The statistical analysis of the data was performed with the Statistical Package for the Social Science, version 20.0RESULTS: It was assessed a larger proportion of children between seven and nine years of age, and both in relation to the stature as the Body Mass Index (BMI). The medians are higher when compared to the reference (World Health Organization), revealing that the children are growing well, even above the average for the benchmark. However, at the same time, they have a BMI higher than expected, even considering their high stature. The diagnosis of nutritional status among the boys and girls showed no statistical differences. However, it is worth highlighting that, although the bigger prevalence is eutrophic children (70.3%), a high prevalence of excess weight (overweight, obesity and severe obesity) can also be observed, around 28% of the sampleCONCLUSION: Although most children were found to be in normal nutritional condition, some were identified as being overweight or obese. It was evident that nutritional condition does not differ in relation to gender, but is influenced by age and height

INTRODUÇÃO: A obesidade vem se tornando cada vez mais frequente em crianças, o que pode resultar em agravos à saúde tanto na infância quanto na idade adulta. Considerando que, na idade escolar, os hábitos alimentares podem sofrer influência do contexto em que a criança está inserida, principalmente, estilo de vida, dentro e fora do ambiente escolar, torna-se importante avaliar o crescimento alcançado em estatura, bem como o seu estado nutricional, visto que este pode ser um indicativo de futuros e possíveis distúrbios nutricionais, permitindo direcionar recursos e políticas públicas efetivas nessa faixa etáriaOBJETIVO: Avaliar o crescimento em estatura e o estado nutricional de crianças em idade escolar em função do sexo e idade em Florianópolis no primeiro semestre do ano letivo de 2012MÉTODO: Estudo de corte transversal, realizado com 595 crianças de seis a dez anos de idade, frequentadoras de Escolas Públicas Municipais de Ensino Fundamental I, na cidade de Florianópolis, Estado de Santa Catarina, Brasil. Utilizou-se um questionário para coleta de dados. Os dados foram digitados e armazenados em planilhas, utilizando o programa Microsoft Excel 2007. A análise estatística dos dados foi realizada com o Statistical Package for the Social Science (SPSS), versão 20.0. O peso e a altura foram inicialmente comparados à curva normal, aplicando-se o teste de distância Kolmogorov-Smirnov. Foram utilizados estatística descritiva e o teste de correlação de PearsonRESULTADOS: Evidenciou-se uma proporção maior de crianças entre os sete e nove anos de idade, e tanto em relação à estatura quanto ao IMC, as medianas estão superiores quando comparadas ao do referencial (Organização Mundial de Saúde), revelando que as crianças estão crescendo bem, inclusive acima da média do referencial, mas que ao mesmo tempo apresentam um IMC acima do esperado, mesmo considerando a elevada estatura. O diagnóstico do estado nutricional entre os meninos e meninas não apresentaram diferenças estatísticas. Contudo, vale destacar que, embora a maior prevalência seja de crianças eutróficas (70,3%), pode-se observar alta prevalência de excesso de peso (sobrepeso, obesidade e obesidade grave), em torno de 28% da amostraCONCLUSÃO: Embora com prevalência do estado nutricional normal, identificou- se o sobrepeso e a obesidade. Evidenciou-se que o estado nutricional não tem diferença em função do sexo, contudo é influenciado pela idade e estatura

Caracterização clínica e sociodemográfica de pessoas estomizadas atendidas em um centro de referência / Clinical and sociodemographic characterization of ostomized patients treated at a referral center

Objetivo: caracterizar o perfil socioclínico epidemiológico da população com estomia atendida em um centro de referência. Métodos: estudo transversal com 255 prontuários do Serviço de Atenção Básica à Pessoa Ostomizada submetidos a uma análise descritiva e estatística (teste qui-quadrado e teste exato de Fisher). Resultados: 54,1% eram homens, 56,5% idosos, 50,2% brancos, 46,7% tinham companheiro, 59,2% com até o ensino fundamental e 34,5% aposentados. Pessoas com colostomia, 71,4%, sendo 33,7% temporárias, 69,4% por neoplasias e 17,3% apresentaram dermatite. Somente uma correlação entre presença de complicações e sexo dos pacientes foi observada (p=0,037). Conclusão: os dados indicam predominância de idosos, do sexo masculino, brancos, com companheiro, aposentados e de baixa escolaridade. Pessoas com colostomia temporárias por neoplasia, com dermatite como principal complicação prevaleceram, tendo uma correlação entre o sexo e a complicação apresentada.(AU)

Bond Strength of Resin Cements Fixing Fiber Posts to Human and Bovine Teeth of Different Ages.

PURPOSE: To evaluate the effect of human and bovine root dentin age on the bond strength of fiber posts fixed with resin cements. The degree of conversion (DC) of the resin cements in different root thirds also was evaluated. MATERIALS AND METHODS: Ninety-six single-rooted teeth were divided into groups according to the origin and age: young human (20-30 years), old human (over 60 years), young bovine (24-36 months) and adult bovine (over 48 months). The teeth were endodontically treated, and fiber posts were cemented with resin cements (RelyX Ultimate and RelyX U200). After 24 h, the teeth were sectioned perpendicularly and the push-out test was performed in a universal testing machine at a speed of 0.5 mm/min, until failure. One specimen from each third from each group (n = 6) was selected, and the DC of the resin cements was analyzed by FT-Raman spectroscopy. RESULTS: For both resin cements, the young human root dentin group presented the highest bond strengths and the old human root dentin group presented the lowest (p < 0.05). The bond strengths of bovine root dentin groups were not affected by aging (p > 0.05). For the DC of resin cements, there were differences among the root thirds (p < 0.05), with the lowest values observed in the apical third. CONCLUSIONS: The bond strengths were higher in the young human root canal than in the bovine substrate, with a negative effect of aging in the human substrate. The DC of dual-curing resin cements decreased from the coronal to apical root thirds.

Tri-Ponderal Mass Index: A Screening Tool for Risk of Central Fat Accumulation in Brazilian Preschool Children.

Background and Objectives: To verify the use of the tri-ponderalmass index (TMI) as a screening tool for risk of central fat accumulation in preschool children. Materials and Methods: An observational, analytical study was carried out on samples from children 2 to 5 years of age. The body mass index (BMI) and the tri-ponderalmass index (TMI: Weight/height3) were calculated. The waist circumference-to-height ratio (WHtR) was used to classify central fat accumulation risk. Preschoolers whose WHtRwas in the upper tertile of the sample were classified as at risk for central fat accumulation. A comparison of the two indicators (BMI and TMI) was made from the area under the receiver operator characteristics (ROC) curve (AUC) in the discrimination of the WHtR. Results: The sample used for analysis was 919 preschoolers. The mean age of the children was 3.9 years (SD = 0.7). The difference in AUC was 5% higher for TMI (p < 0.0001). In the individual analysis of the ROC curve of the TMI, favoring a higher sensitivity, the cutoff point of 14.0 kg/m3 showed a sensitivity of 99.3% (95% CI: 97.6-99.9). Conclusion: Considering WHtR as a marker of possible future metabolic risk among preschool children, TMI proved to be a useful tool, superior to BMI, in screening for risk of central fat accumulation in preschool children.

Implementation of a Brazilian Cardioprotective Nutritional (BALANCE) Program for improvement on quality of diet and secondary prevention of cardiovascular events: A randomized, multicenter trial

Background Complex percutaneous coronary intervention (PCI) is associated with higher ischemic risk, which can be mitigated by long-term dual antiplatelet therapy (DAPT). However, concomitant high bleeding risk (HBR) may be present, making it unclear whether short- or long-term DAPT should be prioritized. Objectives This study investigated the effects of ischemic (by PCI complexity) and bleeding (by PRECISE-DAPT [PRE dicting bleeding Complications in patients undergoing stent Implantation and Sub sequent Dual Anti Platelet Therapy] score) risks on clinical outcomes and on the impact of DAPT duration after coronary stenting. Methods Complex PCI was defined as ≥3 stents implanted and/or ≥3 lesions treated, bifurcation stenting and/or stent length >60 mm, and/or chronic total occlusion revascularization. Ischemic and bleeding outcomes in high (≥25) or non-high (<25) PRECISE-DAPT strata were evaluated based on randomly allocated duration of DAPT. Results Among 14,963 patients from 8 randomized trials, 3,118 underwent complex PCI and experienced a higher rate of ischemic, but not bleeding, events. Long-term DAPT in non-HBR patients reduced ischemic events in both complex (absolute risk difference: −3.86%; 95% confidence interval: −7.71 to +0.06) and noncomplex PCI strata (absolute risk difference: −1.14%; 95% confidence interval: −2.26 to −0.02), but not among HBR patients, regardless of complex PCI features. The bleeding risk according to the Thrombolysis In Myocardial Infarction scale was increased by long-term DAPT only in HBR patients, regardless of PCI complexity. Conclusions Patients who underwent complex PCI had a higher risk of ischemic events, but benefitted from long-term DAPT only if HBR features were not present. These data suggested that when concordant, bleeding, more than ischemic risk, should inform decision-making on the duration of DAPT. (AU)

DNA transposon invasion and microsatellite accumulation guide W chromosome differentiation in a Neotropical fish genome.

Sex chromosome differentiation is subject to independent evolutionary processes among different lineages. The accumulation of repetitive DNAs and consequent crossing-over restriction guide the origin of the heteromorphic sex chromosome region. Several Neotropical fish species have emerged as interesting models for understanding evolution and genome diversity, although knowledge of their genomes is scarce. Here, we investigate the content of repetitive DNAs between males and females of Apareiodon sp. based on large-scale genomic data focusing on W sex chromosome differentiation. In Apareiodon, females are the heterogametic sex (ZW) and males are the homogametic sex (ZZ). The genome size estimate for Apareiodon was 1.2 Gb (with ~ 42× and ~ 47× coverage for males and females, respectively). In Apareiodon sp., approximately 36% of the genome was composed of repetitive DNAs and transposable elements (TEs) were the most abundant class. Read coverage analysis revealed different amounts of repetitive DNAs in males and females. The female-enriched clusters were located on the W sex chromosome and were mostly composed of microsatellite expansions and DNA transposons. Landscape analysis of TE contents demonstrated two major waves of invasions of TEs in the Apareiodon genome. Estimation of TE insertion times correlated with in situ locations permitted the inference that helitron, Tc1-mariner, and CMC EnSpm DNA transposons accumulated repeated copies during W chromosome differentiation between 20 and 12 million years ago. DNA transposons and microsatellite expansions appeared to be major players in W chromosome differentiation and to guide modifications in the genome content of the heteromorphic sex chromosomes.

Nutritional Status Trends in Brazilian Preschoolers: A Cohort Study.

Background: Determining trends in the nutritional status of children may guide care prevention beyond this age in an effort to reduce the prevalence and incidence of overweight and/or obese children. The objective of this study is to evaluate the evolution of the nutritional status of preschool children in two moments, with an interval of 2 years. Methods: This is a cohort study of a random probabilistic sample of preschool children attending public schools within an urban area of high human development index city, in the hinterland of São Paulo state. In 2016, we reassessed the nutritional status of 351 preschoolers evaluated in 2014, comparing the prevalence of overweight according to BMI >1 z-score. Results: The prevalence of overweight was 31.05% (2014) and 31.06% (2016) and mean BMI z-scores were 0.58 and 0.57, respectively. The nutritional status classification of the preschool children showed almost no agreement between the two time points (κ = 0.053). Nevertheless, children with overweight in 2014 had a relative risk of 1.96 of being overweight or obese in 2016 (p = 0.0473). Conclusions: Prevalence of overweight among preschoolers was the same at 2 and 5 years of age, with no tendency to grow. Despite this, 2-year-old preschoolers with overweight present a twofold higher relative risk for excessive weight at 5 years of age. These changes of nutritional status at preschool age evince the great flexibility of their nutritional condition during this period of life.

Potential premalignant status of gastric portion excluded after Roux en-Y gastric bypass in obese women: A pilot study.

We evaluated whether the excluded stomach (ES) after Roux-en-Y gastric bypass (RYGB) can represent a premalignant environment. Twenty obese women were prospectively submitted to double-balloon enteroscopy (DBE) with gastric juice and biopsy collection, before and 3 months after RYGB. We then evaluated morphological and molecular changes by combining endoscopic and histopathological analyses with an integrated untargeted metabolomics and transcriptomics multiplatform. Preoperatively, 16 women already presented with gastric histopathological alterations and an increased pH (≥4.0). These gastric abnormalities worsened after RYGB. A 90-fold increase in the concentration of bile acids was found in ES fluid, which also contained other metabolites commonly found in the intestinal environment, urine, and faeces. In addition, 135 genes were differentially expressed in ES tissue. Combined analysis of metabolic and gene expression data suggested that RYGB promoted activation of biological processes involved in local inflammation, bacteria overgrowth, and cell proliferation sustained by genes involved in carcinogenesis. Accumulated fluid in the ES appears to behave as a potential premalignant environment due to worsening inflammation and changing gene expression patterns that are favorable to the development of cancer. Considering that ES may remain for the rest of the patient's life, long-term ES monitoring is therefore recommended for patients undergoing RYGB.

Percepção de mulheres quanto à prática do aleitamento materno: uma revisão integrativa / Perception of women regarding the practice of breastfeeding: an integrative review / Percepción de la mujer en cuanto a la práctica de la lactancia materna: una revisión integradora

Objetivo: Identificarel conocimiento científico acerca de la percepción de las mujeres con respecto a la lactancia materna. Método: Una revisión integradora para buscar artículos en MEDLINE, Pub / Med, LILACS, BDENF base de datos central de PubMed se realizó: PMCE CINAHL, de 2008 a 2015, se seleccionaron 30 artículos. Resultado: Surgió a partir del análisis las categorías: Beneficios de la lactancia materna; mitos y tabúes que rodean a la lactancia materna; sentimientos contradictorios durante la lactancia, aspectos culturales,la gestión de la lactancia materna y recomendaciones para la práctica. Conclusión:Percepciones de las mujeres sobre la lactancia materna, pueden contribuir a la formulación de recomendaciones con el fin de ayudar a la enfermera en una perspectiva que va más allá de la técnica respetando los significados y prácticas atribuidas a las mujeres que amamantan

Objective: Identify the contribution of the research on the scientific knowledge about the perception of women regarding breastfeeding. Method: An integrative review was carried and the search for articles occurred in MEDLINE, Pub/Med, LILACS, BDENF, PubMed Central: PMCe CINAHL, from 2008 to 2015, containing 30 articles. Results: Emerging from the analysis were the following categories: benefits of breastfeeding, myths and taboos surrounding breastfeeding; contradictory feelings when breastfeeding; cultural aspects and management of breastfeeding. Conclusion: The results indicate aspects about women's perceptions regarding breastfeeding, which may contribute to the development of recommendation sin order to assist the breast feeder in a perspective that goes beyond the technical, respecting the perceptions, meanings and practices attributed to breastfeeding by women

Objetivo: Identificar o conhecimento científico produzido acerca da percepção das mulheres quanto à prática do aleitamento materno.Método:Revisão integrativa com busca dos artigos nas bases de dados MEDLINE, Pub/Med, LILACS, BDENF, PubMed Central: PMC e CINAHL, de 2008 a 2015, sendo selecionados 30 artigos. Resultados: Da análise, emergiram as categorias: vantagens do aleitamento materno; mitos e tabus em torno da amamentação; sentimentos contraditórios ao amamentar; aspectos culturais; manejo da amamentação; e recomendações para a prática. Conclusão: As percepções das mulheres em relação à amamentação podem contribuir para a elaboração de recomendações com vistas a assistir a nutriz numa perspectiva que vai além da técnica, respeitando os significados e práticas atribuídas à amamentação pelas mulheres

Insight into the α-Amylase Inhibitory Activity of Plant Lipid Transfer Proteins.

We previously characterized the inhibitory activity of human salivary α-amylase (HSA) and Callosobruchus maculatus intestinal α-amylases by the plant lipid transfer protein from Vigna unguiculata ( Vu-LTP). Herein, we further study this inhibitory activity. First by an analysis of protein α-amylase inhibitors complexed with α-amylase, we find that positively charged amino acids of inhibitors interact with the active site of α-amylases and we know that Vu-LTP is rich in positively charged amino acid residues. For this reason, we model Vu-LTP, and based on its three-dimensional structure, we choose five peptides to be synthesized. Herein, we report that two peptides of Vu-LTP are responsible for HSA inhibition. A comparison of primary and tertiary structures of LTPs with and without inhibitory activity against α-amylase, superimposed with the sequence of Vu-LTP mapped for HSA inhibition, reinforces our suggestion that positively charged amino acids in loops are responsible for the inhibition. To prove our observation, one modified peptide is synthesized in which Arg39 is replaced by Gln. This modified peptide loses the HSA inhibitory property presented by the unmodified peptide. Therefore, we describe a new biological active for Vu-LTP, i.e. the α-amylase inhibitory activity that is not a fortuitous biological activity and probably has evolved to perform a biological function which is still unknown. A good candidate should be defense against insects. The results of this study also expand the possible biotechnological applications of LTPs.