Employing automatic content recognition for teaching methodology analysis in classroom videos.

A teacher plays a pivotal role in grooming a society and paves way for its social and economic developments. Teaching is a dynamic role and demands continuous adaptation. A teacher adopts teaching techniques suitable for a certain discipline and a situation. A thorough, detailed, and impartial observation of a teacher is a desideratum for adaptation of an effective teaching methodology and it is a laborious exercise. An automatic strategy for analyzing a teacher's teaching methodology in a classroom environment is suggested in this work. The proposed strategy recognizes a teacher's actions in videos while he is delivering lectures. In this study, 3D CNN and Conv2DLSTM with time-distributed layers are used for experimentation. A range of actions are recognized for a complete classroom session during experimentation and the reported results are considered effective for analysis of a teacher's teaching technique.

Computational identification of 4-carboxyglutamate sites to supplement physiological studies using deep learning.

In biological systems, Glutamic acid is a crucial amino acid which is used in protein biosynthesis. Carboxylation of glutamic acid is a significant post-translational modification which plays important role in blood coagulation by activating prothrombin to thrombin. Contrariwise, 4-carboxy-glutamate is also found to be involved in diseases including plaque atherosclerosis, osteoporosis, mineralized heart valves, bone resorption and serves as biomarker for onset of these diseases. Owing to the pathophysiological significance of 4-carboxyglutamate, its identification is important to better understand pathophysiological systems. The wet lab identification of prospective 4-carboxyglutamate sites is costly, laborious and time consuming due to inherent difficulties of in-vivo, ex-vivo and in vitro experiments. To supplement these experiments, we proposed, implemented, and evaluated a different approach to develop 4-carboxyglutamate site predictors using pseudo amino acid compositions (PseAAC) and deep neural networks (DNNs). Our approach does not require any feature extraction and employs deep neural networks to learn feature representation of peptide sequences and performing classification thereof. Proposed approach is validated using standard performance evaluation metrics. Among different deep neural networks, convolutional neural network-based predictor achieved best scores on independent dataset with accuracy of 94.7%, AuC score of 0.91 and F1-score of 0.874 which shows the promise of proposed approach. The iCarboxE-Deep server is deployed at https://share.streamlit.io/sheraz-n/carboxyglutamate/app.py .

iPhosS(Deep)-PseAAC: Identification of Phosphoserine Sites in Proteins Using Deep Learning on General Pseudo Amino Acid Compositions.

Among all the PTMs, the protein phosphorylation is pivotal for various pathological and physiological processes. About 30 percent of eukaryotic proteins undergo the phosphorylation modification, leading to various changes in conformation, function, stability, localization, and so forth. In eukaryotic proteins, phosphorylation occurs on serine (S), Threonine (T) and Tyrosine (Y) residues. Among these all, serine phosphorylation has its own importance as it is associated with various importance biological processes, including energy metabolism, signal transduction pathways, cell cycling, and apoptosis. Thus, its identification is important, however, the in vitro, ex vivo and in vivo identification can be laborious, time-taking and costly. There is a dire need of an efficient and accurate computational model to help researchers and biologists identifying these sites, in an easy manner. Herein, we propose a novel predictor for identification of Phosphoserine sites (PhosS) in proteins, by integrating the Chou's Pseudo Amino Acid Composition (PseAAC) with deep features. We used well-known DNNs for both the tasks of learning a feature representation of peptide sequences and performing classifications. Among different DNNs, the best score is shown by Covolutional Neural Network based model which renders CNN based prediction model the best for Phosphoserine prediction. Based on these results, it is concluded that the proposed model can help to identify PhosS sites in a very efficient and accurate manner which can help scientists understand the mechanism of this modification in proteins.

iGluK-Deep: computational identification of lysine glutarylation sites using deep neural networks with general pseudo amino acid compositions.

Lysine glutarylation is a post-translation modification which plays an important regulatory role in a variety of physiological and enzymatic processes including mitochondrial functions and metabolic processes both in eukaryotic and prokaryotic cells. This post-translational modification influences chromatin structure and thereby results in global regulation of transcription, defects in cell-cycle progression, DNA damage repair, and telomere silencing. To better understand the mechanism of lysine glutarylation, its identification in a protein is necessary, however, experimental methods are time-consuming and labor-intensive. Herein, we propose a new computational prediction approach to supplement experimental methods for identification of lysine glutarylation site prediction by deep neural networks and Chou's Pseudo Amino Acid Composition (PseAAC). We employed well-known deep neural networks for feature representation learning and classification of peptide sequences. Our approach opts raw pseudo amino acid compositions and obsoletes the need to separately perform costly and cumbersome feature extraction and selection. Among the developed deep learning-based predictors, the standard neural network-based predictor demonstrated highest scores in terms of accuracy and all other performance evaluation measures and outperforms majority of previously reported predictors without requiring expensive feature extraction process. iGluK-Deep:Computational Identification of lysine glutarylationsites using deep neural networks with general Pseudo Amino Acid Compositions Sheraz Naseer, Rao Faizan Ali, Yaser Daanial Khan, P.D.D DominicCommunicated by Ramaswamy H. Sarma.

iSUMOK-PseAAC: prediction of lysine sumoylation sites using statistical moments and Chou's PseAAC.

Sumoylation is the post-translational modification that is involved in the adaption of the cells and the functional properties of a large number of proteins. Sumoylation has key importance in subcellular concentration, transcriptional synchronization, chromatin remodeling, response to stress, and regulation of mitosis. Sumoylation is associated with developmental defects in many human diseases such as cancer, Huntington's, Alzheimer's, Parkinson's, Spin cerebellar ataxia 1, and amyotrophic lateral sclerosis. The covalent bonding of Sumoylation is essential to inheriting part of the operative characteristics of some other proteins. For that reason, the prediction of the Sumoylation site has significance in the scientific community. A novel and efficient technique is proposed to predict the Sumoylation sites in proteins by incorporating Chou's Pseudo Amino Acid Composition (PseAAC) with statistical moments-based features. The outcomes from the proposed system using 10 fold cross-validation testing are 94.51%, 94.24%, 94.79% and 0.8903% accuracy, sensitivity, specificity and MCC, respectively. The performance of the proposed system is so far the best in comparison to the other state-of-the-art methods. The codes for the current study are available on the GitHub repository using the link: https://github.com/csbioinfopk/iSumoK-PseAAC.

Optimization of serine phosphorylation prediction in proteins by comparing human engineered features and deep representations.

Deep representations can be used to replace human-engineered representations, as such features are constrained by certain limitations. For the prediction of protein post-translation modifications (PTMs) sites, research community uses different feature extraction techniques applied on Pseudo amino acid compositions (PseAAC). Serine phosphorylation is one of the most important PTM as it is the most occurring, and is important for various biological functions. Creating efficient representations from large protein sequences, to predict PTM sites, is a time and resource intensive task. In this study we propose, implement and evaluate use of Deep learning to learn effective protein data representations from PseAAC to develop data driven PTM detection systems and compare the same with two human representations.. The comparisons are performed by training an xgboost based classifier using each representation. The best scores were achieved by RNN-LSTM based deep representation and CNN based representation with an accuracy score of 81.1% and 78.3% respectively. Human engineered representations scored 77.3% and 74.9% respectively. Based on these results, it is concluded that the deep features are promising feature engineering replacement to identify PhosS sites in a very efficient and accurate manner which can help scientists understand the mechanism of this modification in proteins.