Interaction between functional polymorphisms in FCER1A and TLR2 and the severity of atopic dermatitis.

Dendritic cell toll-like receptors (TLRs) and the high-affinity immunoglobulin E (IgE) receptor (FcεRI) may biologically interact with regard to atopic dermatitis (AD) development and, especially, severity. Our aim here was to test if such interaction can be detected on the genetic level. The combined effect of the TLR2 gene (TLR2) rs4696480 and the FcεRI α-chain gene (FCER1A) rs2252226 and rs2251746 polymorphisms on the AD severity as measured by SCORAD was assessed. The FCER1A rs2252226 and TLR2 rs4696480 polymorphisms interacted with regard to SCORAD. Higher SCORAD was observed in patients being the TLR2 rs4696480 major homozygotes and carrying at the same time the FCER1A rs2252226 minor allele, compared to those characterized by (any other of) the remaining combined rs2252226 and rs4696480 genotypes. The observation of the epistatic effect of TLR2 and FCER1A genetic variants on SCORAD is in line with the involvement of the interaction TLRs-FcεRI in the pathophysiology of AD.

The Impact of Vibration Therapy Interventions on Skin Condition and Skin Temperature Changes in Young Women with Lipodystrophy: A Pilot Study.

BACKGROUND: Cellulite affects 85-98% of women aged over 20 years. In a given context, mechanical vibrations have not been applied in the therapy of gynoid lipodystrophy (GL) until now. The aim of this pilot study was to assess the condition and temperature of skin affected by cellulite after vibration therapy interventions in young women with GL. METHODS: 10 healthy women (21.5 ± 1.5 years old) with stage 1 or 2 Nürnberger-Müller scale of severity of GL participated in the study. The subjects underwent 20 vibration interventions with the use of a Rehabilitation Massage Device Vitberg+. Vibration therapy was applied 5 times a week for 60 minutes during 4-week period. Before and after first and last interventions, grade of lipodystrophy was assessed and thermographic images were taken. RESULTS: Visual and palpation analysis performed before and after a series of treatments proved a total cellulite remission after the intervention among 40% of subjects (from stage 1 to stage 0). Among the remaining 60% with stage 2 of cellulite, an improvement in the skin condition was observed, and cellulite grade was determined as stage 1. The mean skin temperature in the lateral part of thigh as well as on the posterior surface of thigh and buttocks increased significantly (p<0.00001) after both the first (respectively: 4.0°C ± 0.9°C, 3.9°C ± 0.8°C) and the last vibration therapy interventions (respectively: 3.1°C ± 1.1°C, 2.8°C ± 1.1°C). After the series of interventions, a statistical significant (p=0.00705) increase in the mean skin temperature in the lateral thigh was observed-before the first treatment: 27.9°C ± 0.7°C; before twentieth treatment: 29.0°C ± 1.2°C. CONCLUSION: The series of vibration interventions contributed to the reduction of GL among the participants. The thermographic imaging analysis proved an impact of both single and serial vibration interventions.

Plasma fibrin clot properties in atopic dermatitis: links between thrombosis and atopy.

Myocardial infarction and ischemic stroke are associated with formation of dense fibrin clots resistant to lysis. Although pro- and antithrombotic alterations have been reported in atopy, fibrin clot function has not been studied in atopic patients. The aim of the current study was to investigate fibrin clot properties in patients with atopic dermatitis (AD). Plasma fibrin clot permeability, turbidity and clot lysis were assessed in 130 consecutive AD patients, aged 29.7 +/- 11 [+/-SD] years (mean SCORAD index, 32.4 +/- 14.9), free of thrombotic events. A control group comprised 130 healthy controls matched for demographics. Patients with AD had lower clot permeability (7.12 +/- 1.87 vs. 9.32 +/- 0.86 x 10(-9) cm(2); P < 0.0001), increased fiber thickness (maximum clot absorbancy at 405 nm, 4.03 +/- 0.54 vs. 3.47 +/- 0.25), faster clot formation (the lag phase, 39.16 +/- 4.61 vs. 43.05 +/- 4.56 s), higher maximum D-dimer levels released from clots, reflecting increased clot mass (4.05 +/- 0.57 vs. 3.47 +/- 0.25 mg/l; P < 0.0001), lower rate of D-dimer release (0.073 +/- 0.01 vs. 0.078 +/- 0.01 mg/l/min; P < 0.0001), and prolonged fibrinolysis time (9.26 +/- 1.47 vs. 7.81 +/- 1.17 min; P < 0.0001) compared with controls. Concomitant asthma (n = 36; 27.7%) was related to a higher rate of D-dimer release from clots than the remainder (0.075 +/- 0.01 vs. 0.072 +/- 0.01 mg/l/min, respectively; P = 0.03). Altered plasma fibrin clot properties associated with reduced efficiency of fibrinolysis can be detected in AD patients, which might represent a novel mechanism that modulates a hemostatic balance in atopy.

[Ultraviolet radiation, tobacco smoke and estrogens pathways of influence on skin aging; capabilities of prevention]. / Szlaki dzialania UV, dymu papierosowego, estrogenów na proces starzenia sie skóry; mozliwosci prewencji.

Aging refers to the hole human body including the skin, but here it is usually better seen by milieu, repeatedly burdens life quality. There are many theories explaining the process of human aging, but its reasons, irrespectively of their criteria, are numerous and affect one another. Skin aging just like the entire body depends on the influence of genetics, environmental and hormonal factors. Ultraviolet radiation and tobacco smoking have confirmed influence on skin aging. The role of hormonal disorders, particularly estrogens are also underlined. Mechanisms of skin aging induced by UV radiation, tobacco smoke and estrogens are similar and included unfavourable effects of oxidative stress (free radicals) and also disturbances of the TGF beta pathway. Data of many clinical studies proved that avoiding sun and smoking, nucleic acids diet, antioxidant supplementation, everyday use of UV filter, moisturizers, topical use of antioxidants, retinoid derivatives and flavonoids have proved protective the influence to multidirectional process of skin aging.