ABSTRACT
We report a functional assay of Protein C on whole plasma using a snake venom called Protac-C. This method is simple, avoids absorption-elution techniques. Also this method can be adopted to a microtitre plate system testing of large number of samples. Functional levels by this test correlated well with the antigenic levels (r = .8) measured by ELISA. Protein C functional and antigenic values in 58 healthy volunteers were 82% and 95.5% respectively. The warfarinized samples showed a lower mean.
Subject(s)
Peptides/pharmacology , Protein C/analysis , Chromogenic Compounds , Enzyme Activation/drug effects , Enzyme-Linked Immunosorbent Assay , Epitopes/analysis , Humans , Intercellular Signaling Peptides and Proteins , Methods , Protein C/immunology , Protein C/physiology , Time FactorsSubject(s)
Glycoproteins/analysis , Hemostasis , Protein C/analysis , Adult , Antithrombin III/analysis , Blood Coagulation Factors/analysis , Cross-Sectional Studies , Female , Fetal Blood/analysis , Humans , Infant, Newborn , Male , Protein S , alpha-2-Antiplasmin/analysis , alpha-Macroglobulins/analysisABSTRACT
Protein C measurement is now a necessary work-up of a patient with thrombosis. We described an enzyme enhancement of Laurell's immunoelectrophoresis for assay of protein C antigen. With this modification, the rockets are well defined and easily visualized and the sensitivity of the assay increased (2.5%). Samples with low protein C antigen are easily assayed.
