Vitamin D and calcium intakes in general pediatric populations: A French expert consensus paper.

OBJECTIVES: Nutritional vitamin D supplements are often used in general pediatrics. Here, the aim is to address vitamin D supplementation and calcium nutritional intakes in newborns, infants, children, and adolescents to prevent vitamin D deficiency and rickets in general populations. STUDY DESIGN: We formulated clinical questions relating to the following categories: the Patient (or Population) to whom the recommendation will apply; the Intervention being considered; the Comparison (which may be "no action," placebo, or an alternative intervention); and the Outcomes affected by the intervention (PICO). These PICO elements were arranged into the questions to be addressed in the literature searches. Each PICO question then formed the basis for a statement. The population covered consisted of children aged between 0 and 18 years and premature babies hospitalized in neonatology. Two groups were assembled: a core working group and a voting panel from different scientific pediatric committees from the French Society of Pediatrics and national scientific societies. RESULTS: We present here 35 clinical practice points (CPPs) for the use of native vitamin D therapy (ergocalciferol, vitamin D2 and cholecalciferol, vitamin D3) and calcium nutritional intakes in general pediatric populations. CONCLUSION: This consensus document was developed to provide guidance to health care professionals on the use of nutritional vitamin D and dietary modalities to achieve the recommended calcium intakes in general pediatric populations. These CPPs will be revised periodically. Research recommendations to study key vitamin D outcome measures in children are also suggested.

Magneto-mechanical treatment of human glioblastoma cells with engineered iron oxide powder microparticles for triggering apoptosis.

In nanomedicine, treatments based on physical mechanisms are more and more investigated and are promising alternatives for challenging tumor therapy. One of these approaches, called magneto-mechanical treatment, consists in triggering cell death via the vibration of anisotropic magnetic particles, under a low frequency magnetic field. In this work, we introduce a new type of easily accessible magnetic microparticles (MMPs) and study the influence of their surface functionalization on their ability to induce such an effect, and its mechanism. We prepared anisotropic magnetite microparticles by liquid-phase ball milling of a magnetite powder. These particles are completely different from the often-used SPIONs: they are micron-size, ferromagnetic, with a closed-flux magnetic structure reminiscent of that of vortex particles. The magnetic particles were covered with a silica shell, and grafted with PEGylated ligands with various physicochemical properties. We investigated both bare and coated particles' in vitro cytotoxicity, and compared their efficiency to induce U87-MG human glioblastoma cell apoptosis under a low frequency rotating magnetic field (RMF). Our results indicated that (1) the magneto-mechanical treatment with bare MMPs induces a rapid decrease in cell viability whereas the effect is slower with PEGylated particles; (2) the number of apoptotic cells after magneto-mechanical treatment is higher with PEGylated particles; (3) a lower frequency of RMF (down to 2 Hz) favors the apoptosis. These results highlight a difference in the cell death mechanism according to the properties of particles used - the rapid cell death observed with the bare MMPs indicates a death pathway via necrosis, while PEGylated particles seem to favor apoptosis.

Optical response of magnetically actuated biocompatible membranes.

Biocompatible suspended magneto-elastic membranes were prepared. They consist of PDMS (polydimethylsiloxane) films, with embedded arrays of micrometric magnetic pillars made with lithography techniques. For visible light wavelengths, our membranes constitute magnetically tunable optical diffraction gratings, in transmission and reflection. The optical response has been quantitatively correlated with membrane structure and deformation, through optical and magneto-mechanical models. In contrast to the case of planar membranes, the diffraction patterns measured in reflection and transmission vary very differently upon magnetic field application. Indeed, the reflected beam is largely affected by the membrane bending, whereas the transmitted beam remains almost unchanged. In reflection, even weak membrane deformation can produce significant changes of the diffraction patterns. This field-controlled optical response may be used in adaptive optical applications, photonic devices, and for biological applications.

Coherent frequency conversion in a superconducting artificial atom with two internal degrees of freedom.

By adding a large inductance in a dc-SQUID phase qubit loop, one decouples the junctions' dynamics and creates a superconducting artificial atom with two internal degrees of freedom. In addition to the usual symmetric plasma mode (s mode) which gives rise to the phase qubit, an antisymmetric mode (a mode) appears. These two modes can be described by two anharmonic oscillators with eigenstates |ns> and |na> for the s and a mode, respectively. We show that a strong nonlinear coupling between the modes leads to a large energy splitting between states |0s,1a> and |2s,0a>. Finally, coherent frequency conversion is observed via free oscillations between the states |0s,1a> and |2s,0a>.

Electronic structure of epitaxial graphene layers on SiC: effect of the substrate.

A strong substrate-graphite bond is found in the first all-carbon layer by density functional theory calculations and x-ray diffraction for few graphene layers grown epitaxially on SiC. This first layer is devoid of graphene electronic properties and acts as a buffer layer. The graphene nature of the film is recovered by the second carbon layer grown on both the (0001) and (0001[over]) 4H-SiC surfaces. We also present evidence of a charge transfer that depends on the interface geometry. Hence the graphene is doped and a gap opens at the Dirac point after three Bernal stacked carbon layers are formed.

Raman spectroscopy of free-standing individual semiconducting single-wall carbon nanotubes.

The radial breathing modes and tangential modes have been systematically measured on a large number of individual semiconducting single-wall carbon nanotubes (thin bundles) suspended between plots (free-standing single-wall carbon nanotubes). The strong intensity of the Raman spectra ensures the precision of the experimentally determined line shapes and frequencies of these modes. The diameter dependence of the frequencies of the tangential modes was measured. This dependence is discussed in relation with recent calculations. The present data confirm/contradict some previous interpretations.

Aharonov-Bohm cages in 2D normal metal networks.

We report on magnetoresistance transport measurements performed on a bipartite tiling of rhombus in the GaAs/GaAlAs system. We observe for the first time large amplitude h/e oscillations in this network as compared to the one measured in square lattices of similar size. These oscillations are the signature of a recently predicted localization phenomenon induced by Aharonov-Bohm interferences in this peculiar network.

Noninterference of cytochrome P4501A2 in the cytotoxicity of tacrine using genetically engineered V79 Chinese hamster cells for stable expression of the human or rat isoform and two human hepatocyte cell lines.

Tacrine (THA) is the only drug currently approved for the treatment of Alzheimer's disease. A common side effect of this drug in humans is major hepatotoxicity. THA-induced toxicity may be related to a metabolic pathway implicating cytochrome P450 1A2 (CYP1A2). The purpose of this study was to clarify the role of the metabolic conversion of THA by CYP1A2 in the cytotoxicity of THA. The cytotoxicity of THA was evaluated in two human hepatocyte cell lines, HepG2 and Chang liver, and on the V79 Chinese hamster cell line, which does not express cytochrome P450 activity, and its variants, genetically engineered for expression of human or rat CYP1A2. Cells expressing human CYP1A2 metabolized THA to form its 1-OH derivative (Vmax = 9.36 +/- 0.57 pmol min(-1) mg(-1) total protein), whereas no metabolism was observed with the nonexpressing parental cells. In all cell lines, THA induced a marked decrease in cell viability and a strong inhibition of RNA and protein synthesis. However, these cytotoxic effects did not differ in parental V79 cells and variant cells expressing human or rat CYP1A2. The IC50 were tenfold higher for cell viability than for RNA and protein inhibition after 3 hr of incubation but were similar after 24 hr (P < 0.0001), indicating that this early inhibition was not a transient effect and could lead to cell death. These results strongly suggest that THA-induced cytotoxicity is not mediated by CYP1A2.

[No relationship between tacrine cytotoxicity and its metabolisation by cytochrome P4501A2]. / Absence de corrélation entre la cytotoxicité de la tacrine et sa métabolisation par le cytochrome P4501A2.

It has been suggested that tacrine (THA) induced hepatotoxicity was related to its metabolic pathway involving cytochrome P4501A2 (CYP1A2). Using a model of genetically modified cells we have demonstrated that THA induced a marked decrease in cell viability and a strong inhibition of RNA and protein synthesis. However, these cytotoxic effects did not differ in parental Chinese hamster V79 cells and variant cells expressing human or rat CYP1A2 as well in human HepG2 and Chang Liver cells despite their notable metabolism ability to metabolize THA to hydroxylated metabolites. These results strongly suggest that THA-induced cytotoxicity is not mediated by CYP1A2 indicating that THA could be toxic by direct inhibition at the ribosomial level.

[Fluorescence of shade guides]. / Fluorescence des nuanciers.

The experimental protocol developed in the previous article (4) has been modified to permit the investigation of the fluorescence of shade guides. The following will be traced out on each graphic display: a reference spectrum using full lines; a sample spectrum using dotted lines; the chromaticity triangle of the X Y Z CIE 1931 system; the chromaticity of the sample to be tested, represented by the sign +; the chromaticity of the reference, represented by the sign -. The shade guides without any rare earth have spectra in the form of wide bands; their intensity decreases when the degree of colour purity in white light of the buttons increases. The shade guides containing rare earth have spectra with both a wide band and thin lines. It has been shown that: the same shade guide can contain different fluorescence components; two shade guides from the same source can have very different fluorescences for buttons of the same reference; two shade guides from different sources can have identical fluorescent components. This study reveals: that unadapted fluorescences of shade guide may be the cause of an error in the choice of the colour of a ceramic restoration; that the comparison of spectra appears to prove that shade guides are not manufactured by the ceramic manufacturers which recommend them and are, in fact, of the same origin.

[Fluorescence of dental porcelain: material and methods]. / Etude de la fluorescence de la porcelaine dentaire: matériel et méthodes.

Dental porcelain emits some fluorescence under the action of ultra-violet rays. This emission may be at the origin of errors in the choice of the colour of a crown. In order to study this fluorescence phenomenon, the following experimental protocol has been developed: 363.8 nm exciting radiation isolated from the emission by an Argon laser; Fluorescence emitted by the sample and dispersed via a spectrometer, protected by a stop-U.V. filter; Influx collected by a photomultiplier, then directed, after passage in a picoamperemeter, toward a mini-computer programmed to print the spectra; Correction of the spectra by a tungsten lamp used at the 2,600 K colour temperature; Use of reference spectra. On the same graph, the sample spectra are represented in solid lines, while the spectrum of the enamel used as a reference is shown as a dotted line. The results show that: Enamel has a fluorescence spectrum which has the shape of a wide band, with a maximum of 450 nm (characteristic of a blue-green shade) and a slow decrease up to 680 nm. The enamel fluorescence does not depend on the colour of the tooth; Dentine has a distribution spectrum which is similar to that of enamel but is three times fuller; The spectra of the ceramic samples reveal: a wide band due to transition metals, fine lines due to rare earth (terbium and europium). When the saturation degree of the ceramic increases, its fluorescence colour varies due to the relative increase in the amplitude of the lines in relation to the bands. Thus, when the sample colour progresses from B1 to B4, its fluorescence colour becomes greener.(ABSTRACT TRUNCATED AT 250 WORDS)

Mode of inheritance in familial cases of primary gonadotropic deficiency.

The mode of inheritance of primary gonadotropic deficiency was studied in 38 children and adolescents. 92% of this population was male with high frequencies of undescended testes (80%) and micropenis (31%). Anosmia was present in 61% of the patients aged more than 5 years and was a frequent genetic marker in the families. Inheritance was matrilineal in 18, X-linked dominant or autosomal dominant in 6. In 13 cases, the transmission was patrilineal and evoked autosomal dominant inheritance. An autosomal recessive transmission was likely in 7 patients. The data agree with the suggestion of multiple modes of inheritance of congenital gonadotropic deficiency, and clearly show the wide range of expressivity of the disorder.

[Normal male phenotype or hypospadias and female karyotype. XX male syndrome in children and adolescents]. / Phénotype masculin normal ou hypospade et caryotype féminin. Le syndrome des mâles XX chez l'enfant et l'adolescent.

A morphologically normal 46 XX karyotype has been found in 8 patients with male phenotype, either normal (3 cases) or hypospadiac (5 cases) studied at age 1 month to 15 years. Five had cryptorchidism. Pubertal follow-up was obtained in 6 patients and showed that they had hypogonadism with small testes, and a mean adult height of 163 cm. The hormonal investigations gave normal results before puberty, then after the onset of puberty a hypergonadotropic hypogonadism. Testicular biopsy showed alterations from age 8 years. Studies using Y-specific probes in 3 patients have shown the presence of Y genetic material in 2 and absence in 1, thus demonstrating genetic heterogeneity of the XX males.