Bioaccumulated trace element toxicity in commercially harvested bronze whaler sharks (Carcharhinus brachyurus) of South Africa.

Shark meat consumption may pose a significant risk to human health as high levels of toxic pollutants bioaccumulate in muscular tissue. Commercial harvest of Carcharhinus brachyurus meat in South Africa is estimated at 100-300 filleted tons per annum. Muscle tissue samples from 41 sharks were collected from the southern and eastern coastlines of South Africa in 2022 and analysed for 10 trace elements and 8 polychlorinated biphenyl congeners. All trace elements were found to be lower than the regulatory maximum limits for human consumption in most samples irrespective of shark length, sex, and sampling region. However, the estimated daily intake for Mercury and Arsenic exceeded the oral reference dose set by international agencies. The meat from this shark may be consumed due to its low toxic potential for human health, however long-term exposure to C. brachyurus meat should be avoided as it could pose detrimental health risks to consumers.

Leopard subspecies conservation under climate and land-use change.

Predicting the effects of global environmental changes on species distribution is a top conservation priority, particularly for large carnivores, that contribute to regulating and maintaining ecosystems. As the most widespread and adaptable large felid, ranging across Africa and Asia, leopards are crucial to many ecosystems as both keystone and umbrella species, yet they are threatened across their ranges. We used intraspecific species distribution models (SDMs) to predict changes in range suitability for leopards under future climate and land-use change and identify conservation gaps and opportunities. We generated intraspecific SDMs for the three western leopard subspecies, the African, Panthera pardus pardus; Arabian, Panthera pardus nimr; and Persian, Panthera pardus tulliana, leopards, and overlapped predictions with protected areas (PAs) coverage. We show that leopard subspecies differ in their environmental associations and vulnerability to future changes. The African and Arabian leopards are predicted to lose ~25% and ~14% of their currently suitable range, respectively, while the Persian leopard is predicted to experience ~12% range gains. We found that most areas predicted to be suitable were not protected, with only 4%-16% of the subspecies' ranges falling inside PAs, and that these proportions will decrease in the future. The highly variable responses we found between leopard subspecies highlight the importance of considering intraspecific variation when modelling vulnerability to climate and land-use changes. The predicted decrease in proportion of suitable ranges falling inside PAs threatens global capacity to effectively conserve leopards because survival rates are substantially lower outside PAs due to persecution. Hence, it is important to work with local communities to address negative human-wildlife interactions and to restore habitats to retain landscape connectivity where PA coverage is low. On the other hand, the predicted increase in range suitability across southern Europe presents opportunities for expansion outside of their contemporary range, capitalising on European rewilding schemes.

Contemporary Cultural Trade of Lion Body Parts.

Trade in lion parts associated with cultural and traditional use is poorly understood. Here we sought to better understand the role and use of lion body parts in the commercial traditional medicine (muthi) trade of South Africa. In 2019 we conducted a semi-structured questionnaire survey of muthi traders (n = 10) and traditional healers (n = 20) which explored the significance and symbolism of lions, traded parts and preferences, sources and supply of lion parts, and perceived sustainability of lion derivatives in the South African muthi trade. Our results suggest a cultural importance of lion associated with the umndawu ancestral spirit in particular, as well as in the training and practice of cultural-spiritual healers. Lion paws and parts thereof were most frequently reported as sold by traders and demanded by healers, correlating with recent trends in body-part removals from lion mortalities. Respondents indicated that lion parts were obtained from a variety of sources including wild lion populations in neighbouring countries and captive-breeding farms. Our findings are discussed relative to current concerns in lion conservation and highlight a need for further understanding of the traditional medicine complex, the influence that ancestral spirits have on lion body-part trade, and increased engagement with traditional medicine stakeholders.

Genome report: chromosome-level draft assemblies of the snow leopard, African leopard, and tiger (Panthera uncia, Panthera pardus pardus, and Panthera tigris).

The big cats (genus Panthera) represent some of the most popular and charismatic species on the planet. Although some reference genomes are available for this clade, few are at the chromosome level, inhibiting high-resolution genomic studies. We assembled genomes from 3 members of the genus, the tiger (Panthera tigris), the snow leopard (Panthera uncia), and the African leopard (Panthera pardus pardus), at chromosome or near-chromosome level. We used a combination of short- and long-read technologies, as well as proximity ligation data from Hi-C technology, to achieve high continuity and contiguity for each individual. We hope that these genomes will aid in further evolutionary and conservation research of this iconic group of mammals.

Genetic guidelines for translocations: Maintaining intraspecific diversity in the lion (Panthera leo).

Conservation translocations have become an important management tool, particularly for large wildlife species such as the lion (Panthera leo). When planning translocations, the genetic background of populations needs to be taken into account; failure to do so risks disrupting existing patterns of genetic variation, ultimately leading to genetic homogenization, and thereby reducing resilience and adaptability of the species. We urge wildlife managers to include knowledge of the genetic background of source/target populations, as well as species-wide patterns, in any management intervention. We present a hierarchical decision-making tool in which we list 132 lion populations/lion conservation units and provide information on genetic assignment, uncertainty and suitability for translocation for each source/target combination. By including four levels of suitability, from 'first choice' to 'no option', we provide managers with a range of options. To illustrate the extent of international trade of lions, and the potential disruption of natural patterns of intraspecific diversity, we mined the CITES Trade Database for estimated trade quantities of live individuals imported into lion range states during the past 4 decades. We identified 1056 recorded individuals with a potential risk of interbreeding with wild lions, 772 being captive-sourced. Scoring each of the records with our decision-making tool illustrates that only 7% of the translocated individuals were 'first choice' and 73% were 'no option'. We acknowledge that other, nongenetic factors are important in the decision-making process, and hence a pragmatic approach is needed. A framework in which source/target populations are scored based on suitability is not only relevant to lion, but also to other species of wildlife that are frequently translocated. We hope that the presented overview supports managers to include genetics in future management decisions and contributes towards conservation of the lion in its full diversity.

Measuring the intensity of conflicts in conservation.

Conflicts between the interests of biodiversity conservation and other human activities pose a major threat to natural ecosystems and human well-being, yet few methods exist to quantify their intensity and model their dynamics. We develop a categorization of conflict intensity based on the curve of conflict, a model originally used to track the escalation and deescalation of armed conflicts. Our categorization assigns six intensity levels reflecting the discourse and actions of stakeholders involved in a given conflict, from coexistence or collaboration to physical violence. Using a range of case studies, we demonstrate the value of our approach in quantifying conflict trends, estimating transition probabilities between conflict stages, and modeling conflict intensity as a function of relevant covariates. By taking an evidence-based approach to quantifying stakeholder behavior, the proposed framework allows for a better understanding of the drivers of conservation conflict development across a diverse range of socioecological scenarios.

Monitoring compliance of CITES lion bone exports from South Africa.

From 2008 to 2018, South Africa permitted the export of captive-bred African lion (Panthera leo) skeletons to Southeast Asia under CITES Appendix II. Legal exports rose from approximately 50 individuals in 2008 to a maximum of 1,771 skeletons in 2016, and has led to ongoing concerns over possible laundering of non-lion, multiple-source and wild-sourced bones. South Africa is required under its obligations to CITES to employ mechanisms for monitoring and reporting trade, and to limit the potential for illegal trade and laundering of lion and other large felid bones. Monitoring tools for legal trade are critical to compliance with CITES. Here we evaluate the CITES-compliance procedure implemented by South Africa for export of lion bones and identify six essential general points for consideration in the implementation of animal export quota compliance protocols. We provide specific insight into the South African lion bone export monitoring system through: i) outlining the protocols followed; ii) assessing the utility of cranial morphology to identify species; iii) evaluating skeleton consignment weight as a monitoring tool; and iv) presenting molecular (DNA) species assignment and pairwise-comparative sample matching of individuals. We describe irregularities and illicit behaviour detected in the 2017 and 2018 lion bone quotas. Notably, we report that the compliance procedure successfully identified and prevented the attempted laundering of a tiger (P. tigris) skeleton in 2018. We emphasise the utility of mixed-method protocols for the monitoring of compliance in CITES Appendix II export quota systems.

Unsustainable anthropogenic mortality disrupts natal dispersal and promotes inbreeding in leopards.

Anthropogenic mortality of wildlife is typically inferred from measures of the absolute decline in population numbers. However, increasing evidence suggests that indirect demographic effects including changes to the age, sex, and social structure of populations, as well as the behavior of survivors, can profoundly impact population health and viability. Specifically, anthropogenic mortality of wildlife (especially when unsustainable) and fragmentation of the spatial distribution of individuals (home-ranges) could disrupt natal dispersal mechanisms, with long-term consequences to genetic structure, by compromising outbreeding behavior and gene flow. We investigate this threat in African leopards (Panthera pardus pardus), a polygynous felid with male-biased natal dispersal. Using a combination of spatial (home-range) and genetic (21 polymorphic microsatellites) data from 142 adult leopards, we contrast the structure of two South African populations with markedly different histories of anthropogenically linked mortality. Home-range overlap, parentage assignment, and spatio-genetic autocorrelation together show that historical exploitation of leopards in a recovering protected area has disrupted and reduced subadult male dispersal, thereby facilitating opportunistic male natal philopatry, with sons establishing territories closer to their mothers and sisters. The resultant kin-clustering in males of this historically exploited population is comparable to that of females in a well-protected reserve and has ultimately led to localized inbreeding. Our findings demonstrate novel evidence directly linking unsustainable anthropogenic mortality to inbreeding through disrupted dispersal in a large, solitary felid and expose the genetic consequences underlying this behavioral change. We therefore emphasize the importance of managing and mitigating the effects of unsustainable exploitation on local populations and increasing habitat fragmentation between contiguous protected areas by promoting in situ recovery and providing corridors of suitable habitat that maintain genetic connectivity.