First database of the spatial distribution of Eimeria species of cattle, sheep and goats in Mexico.

Ruminant coccidiosis is a gastrointestinal disease caused by parasites of the genus Eimeria. Environmental and climatic factors are relevant for the development, survival, and transmission of coccidiosis because Eimeria oocysts are able to survive in the environment for several weeks or months in favorable conditions of moderate heat and moisture. The aim of the present study was to georeference, for the first time, the locations of Eimeria occurrences in Mexico from 1961 to 2018. A dataset was created for 3414 reports of Eimeria occurrences in cattle, sheep, and goats in Mexico. Twelve species of Eimeria that infect cattle were recorded, 11 Eimeria species of sheep are present in Mexico, and eight species of goats are geographically distributed in the country. In the current findings, it has been observed that Eimeria colonizes mainly the temperate semihumid, temperate humid, warm humid, and warm semihumid areas during spring and summer in animals younger than 1 year of age. Macroenvironmental variables like temperature and rainfall influence the prevalence of Eimeria in cattle, sheep, and goats, and for some species, the rearing system, facility type, farm size, and altitude affect the occurrence of this parasite. Results may support future studies aimed at reducing the disease prevalence of the parasite in endemic regions of Mexico. The use of recorded cases and climate variables yields a more comprehensive perspective of the epidemiology of eimeriosis, which would be difficult to infer from laboratory studies alone.

Anticoccidial efficacy of naringenin and a grapefruit peel extract in growing lambs naturally-infected with Eimeria spp.

The current study aimed to determine the anti-Eimeria efficacy of an extract of grapefruit peels (GF) and commercial naringenin (NAR) in naturally-infected lambs, as well as the influence of these flavonoids on the oxidative status during ovine coccidiosis. Pharmacokinetic profiles were also determined. Extracts were administered per os to Eimeria naturally infected growing lambs during 90 consecutive days. The commercial anticoccidial drug toltrazuril (TTZ) was included in this trial as a standard. Twenty-four lambs were divided into four groups: NAR, lambs given a daily dose of 5mg of a commercial naringenin extract of 98% higher purity per kg body weight; GF, lambs that recived a daily dose of 5mg of ethanolic extract of grapefruit peels per kg body weight; TTZ, lambs treated with 20mg of toltrazuril/kg body weight on days 0 and 15 of the experiment; and CTRL, untreated lambs that received daily dose of 30ml of water. Daily doses of GF and NAR were dissolved in 30ml of water and orally given to animals; whereas toltrazuril was administered as a single dose of an undiluted suspension to lambs of the TTZ group. The CTRL group received 30ml of water; as well as the TTZ group for the period after the single dose administration. Fecal and serum samples were collected from all lambs. Anticoccidial efficacy was estimated by coprological techniques. Generation of nitric oxide levels and the antioxidant capacity of the experimental compounds were determined by the Griess and ABTS assays, respectively. The pharmacokinetic parameters of NAR and the GF extract were obtained. On day 30 post-ingestion, anticoccidial efficacy was 91.76% (NAR) and 89.65% (GF); whereas 99.63% of efficacy was achieved with TTZ 15days after treatment. NAR, GF and TTZ significantly reduced oxidative stress in infected animals. The mean daily weight gain for each group was 122g (NAR), 122g (GF), 143g (TTZ) and 98g (CTRL). Following the oral administration of NAR and GF, values in plasma approached maximum concentrations within 2.1 to 2.5h. In conclusion, the administration of NAR and the GF extract reduced Eimeria oocyst output, oxidative stress and promoted higher mean daily weight gains in infected lambs.

Effect of a cysteine protease inhibitor on Fasciola hepatica (liver fluke) fecundity, egg viability, parasite burden, and size in experimentally infected sheep.

Fasciola hepatica secretes proteolytic enzymes during liver invasion. The present study examined the effects of the cysteine protease inhibitor Ep-475 on sheep considering the following variables: serum levels of aspartate aminotransferase, L-lactate dehydrogenase, and gamma-glutamyltransferase, liver fluke fecundity, egg viability, parasite burden, and size. Twenty-four male sheep were randomly allocated in four groups of six animals each as follows: group A was infected with F. hepatica metacercariae and treated with 50 mg/kg of Ep-475, group B was infected and untreated, group C was uninfected and treated, and group D was uninfected and untreated. All animals were euthanized 10 weeks after the experimental infection. Serum activities of enzymes in infected animals were significantly lower in Ep-475-treated sheep than in untreated controls, although liver damage was produced. No significant reduction in total worm burden was observed between treated and untreated sheep. However, there was a significant difference on the average size, structure development, ova counts, and egg viability of liver flukes from these two groups. Results showed that Ep-475 reduces liver damage due to fasciolosis and induces an impairment of liver fluke growth and fecundity. These effects pinpoint liver fluke proteases as potential targets for pharmacological intervention.

Dose-response inhibition of proteolytic activity by a cysteine protease inhibitor in a murine model of fasciolosis.

Using the film in situ zymography (FIZ) technique, it has been demonstrated that N-[N-(L: -3-trans-carboxyoxirane-2-carbonyl)-L: -leucyl]-agmatine (E-64) inhibits Fasciola hepatica proteolytic activity in vivo. The aim of this study was to establish the dose-response relationship of the inhibition of proteolysis as assessed by FIZ with E-64 at different dosages in a murine model of fasciolosis. Maximum effective inhibition of proteolysis was achieved at 50 mg/kg/day (87%). Mice treated with this dose survived for a mean of 10.92 days more than untreated controls, and their ova counts and egg viability were significantly (P<0.05) lower than this latter group. These results indicate that intraperitoneal administration of E-64 not only inhibited liver proteolytic activity in a dose-dependent manner but also produced anti-fecundity and anti-embryonation effects, delaying the progression of fasciolosis. Yet, residual proteolysis may suggest that other E-64-non-sensitive enzymes are involved, or that E-64-enzyme chemical interactions are only capable of a partial agonistic-like effect.

Frecuencia de las cosechas en Cymbopogon citratus Stapf en las condiciones edafoclimáticas de La Habana / Frequency of the crops in Cymbopogon citratus Stapf under the edaphic and climatic conditions of Havana province

Mediante un experimento de campo, sobre un suelo ferralítico rojo de La Habana, se evaluó el efecto de tres frecuencias de cosecha: tres, cuatro y cinco cosechas anuales. Las variables estudiadas fueron: altura y número de hijos por planta, plantas muertas por parcela, rendimiento en masa vegetal, contenido y calidad del aceite esencial. Los resultados indicaron que cuatro cosechas al año, cada tres meses resultó conveniente para el Cymbopogon citratus Stapf, ya que aportó los más altos rendimientos, sin que se afectara la calidad del aceite esencial(AU)

Efecto de diferentes densidades de plantación en Cymbopogon citratus Stapf / Effect of different plantation densities in Cymbopogon citratus Stapf

Se evaluó el efecto de diferentes densidades de plantación sobre el crecimiento, desarrollo y rendimiento en masa vegetal de Cymbopogon citratus. Para ello se realizó un experimento en la Estación Experimental de Aceites Esenciales de la Unión de Empresas Suchel, sobre un suelo ferralítico rojo entre 1990 y 1991. Se estudiaron las siguientes densidades: 37 037, 22 222, 12 345, 6 172 y 3 082 plantas por hectárea. Se empleó un diseño de bloques al azar. Se pudo concluir que las densidades de plantación de 37 037 y 22 222, dieron los mayores rendimientos de masa vegetal(AU)

Efecto de diferentes densidades de plantación en Cymbopogon citratus Stapf / Effect of different plantation densities in Cymbopogon citratus Stapf

Se evaluó el efecto de diferentes densidades de plantación sobre el crecimiento, desarrollo y rendimiento en masa vegetal de Cymbopogon citratus. Para ello se realizó un experimento en la Estación Experimental de Aceites Esenciales de la Unión de Empresas Suchel, sobre un suelo ferralítico rojo entre 1990 y 1991. Se estudiaron las siguientes densidades: 37 037, 22 222, 12 345, 6 172 y 3 082 plantas por hectárea. Se empleó un diseño de bloques al azar. Se pudo concluir que las densidades de plantación de 37 037 y 22 222, dieron los mayores rendimientos de masa vegetal

Frecuencia de las cosechas en Cymbopogon citratus Stapf en las condiciones edafoclimáticas de La Habana / Frequency of the crops in Cymbopogon citratus Stapf under the edaphic and climatic conditions of Havana province

Mediante un experimento de campo, sobre un suelo ferralítico rojo de La Habana, se evaluó el efecto de tres frecuencias de cosecha: tres, cuatro y cinco cosechas anuales. Las variables estudiadas fueron: altura y número de hijos por planta, plantas muertas por parcela, rendimiento en masa vegetal, contenido y calidad del aceite esencial. Los resultados indicaron que cuatro cosechas al año, cada tres meses resultó conveniente para el Cymbopogon citratus Stapf, ya que aportó los más altos rendimientos, sin que se afectara la calidad del aceite esencial