[Validation of an in-house method for the determination of zinc in serum: Meeting the requirements of ISO 17025]. / Propuesta de un procedimiento de validación de un método para la determinación de cinc sérico: cumplimiento de los requisitos de la ISO 17025.

OBJECTIVE: The aim of this report is to propose a scheme for validation of an analytical technique according to ISO 17025. MATERIAL AND METHODS: According to ISO 17025, the fundamental parameters tested were: selectivity, calibration model, precision, accuracy, uncertainty of measurement, and analytical interference. RESULTS: A protocol has been developed that has been applied successfully to quantify zinc in serum by atomic absorption spectrometry. CONCLUSION: It is demonstrated that our method is selective, linear, accurate, and precise, making it suitable for use in routine diagnostics.