Oral beta-hydroxybutyrate alleviates COVID-19 related acute respiratory distress syndrome: A randomized, single-blind, placebo-controlled trial.

BACKGROUND: Acute respiratory distress syndrome (ARDS) is a lung complication of COVID-19 that requires intensive care and ventilation. Beta-hydroxybutyrate (BHB) is a ketone body that can modulate metabolism and inflammation in immune cells and lung tissues. We hypothesized that oral BHB could alleviate COVID-19 related ARDS by reducing pro-inflammatory cytokines and increasing anti-inflammatory cytokines. METHODS: We randomized 75 patients with mild (as per Berlin criteria) ARDS symptoms to receive oral 25 g twice daily or placebo for five days. The primary outcome was the change in pro-inflammatory cytokines (Interleukin-1ß, Interleukin-6, interleukin-18, tumour necrosis factor-alpha) and anti-inflammatory cytokine (interleukin-10) from baseline to day 5. The secondary outcomes were the change in BHB levels from baseline to day 5, the number of hospitalization days, and the occurrence of adverse events. RESULTS: Treatment with formulated BHB resulted in a significant decrease in pro-inflammatory cytokines; Interleukin-1ß (p = 0.0204), Interleukin-6 (p = 0.0309), interleukin-18 (p = 0.0116), tumour necrosis factor-alpha (p = 0.0489) and increase in interleukin-10 (p = 0.0246) compared treatment with placebo. Importantly, higher BHB levels (p = 0.0001) were observed after supplementation; additionally, patients who underwent this approach were hospitalized for fewer days. No serious adverse events were reported. CONCLUSION: Beta-hydroxybutyrate, an oral adjunct therapy, has shown promising results in ameliorating symptoms of ARDS. This includes reduced inflammation, oxidative stress, and decreased patient fatigue levels. Further study with a large sample size is warranted to assess the potential of BHB therapy's effectiveness in reducing the development of severe illness. CLINICAL TRIAL REGISTRATION: (http://ctri.nic.in/CTRI/2021/03/031790).

Propellant Free Pressurized Spray System of Etodolac to Manage Acute Pain Conditions: In Vitro and In Vivo Evaluation.

This study aimed to develop a propellant-free topical spray formulation of Etodolac (BCS-II), a potent NSAID, which could be beneficial in the medical field for the effective treatment of pain and inflammation conditions. The developed novel propellant-free spray formulation is user-friendly, cost-effective, propellant-free, eco-friendly, enhances the penetration of Etodolac through the skin, and has a quick onset of action. Various formulations were developed by adjusting the concentrations of different components, including lecithin, buffering agents, film-forming agents, plasticizers, and permeation enhancers. The prepared propellant-free spray formulations were then extensively characterized and evaluated through various in vitro, ex vivo, and in vivo parameters. The optimized formulation exhibits an average shot weight of 0.24 ± 0.30 ml and an average drug content or content uniformity of 87.3 ± 1.01% per spray. Additionally, the optimized formulation exhibits an evaporation time of 3 ± 0.24 min. The skin permeation study demonstrated that the permeability coefficients of the optimized spray formulation were 21.42 cm/h for rat skin, 13.64 cm/h for mice skin, and 18.97 cm/h for the Strat-M membrane. When assessing its potential for drug deposition using rat skin, mice skin, and the Strat-M membrane, the enhancement ratios for the optimized formulation were 1.88, 2.46, and 1.92, respectively against pure drug solution. The findings from our study suggest that the propellant-free Etodolac spray is a reliable and safe topical formulation. It demonstrates enhanced skin deposition, and improved effectiveness, and is free from any skin irritation concerns.

Use of Total Parenteral Nutrition (TPN) as a Vehicle for Drug Delivery.

Total Parenteral Nutrition (TPN) is a method of providing nutrients directly into the bloodstream for individuals who are unable to meet their nutritional needs through the normal digestive process or gastrointestinal system. It provides macronutrients and micronutrients in a single container, reducing handling and contamination risks and making it more cost-effective. TPN has the potential to be used as a drug delivery system, with applications in combination therapies, personalized medicine, and integrating advanced technologies. It can enhance drug dosage precision and provide nutritional assistance, potentially reducing hospitalization and improving patient outcomes. However, implementing new applications requires thorough testing and regulatory approval. TPN could be particularly useful in pediatric and geriatric care and could also contribute to global health by combating malnutrition in areas with limited medical resources. Healthcare professionals prepare a sterile solution tailored to each patient's nutritional needs, and administration involves a central venous catheter. However, the simultaneous administration of medications with PN admixtures can result in pharmacological incompatibility, which can impact the stability of the oil-in-water system. The European Society for Clinical Nutrition and Metabolism and the American Society for Parenteral and Enteral Nutrition recommendations advise against including non-nutrient drugs in PN admixtures due to safety concerns. This review focuses on the utilization of Total Parenteral Nutrition (TPN) as a method for delivering drugs. It discusses the benefits and difficulties associated with its commercial application and offers suggestions for future research endeavors.

Biofilm and suspension-based cultivation of microalgae to treat anaerobic digestate food effluent (ADFE).

Anaerobic digestion of organic waste produces effluent (ADE) that requires further treatment. Biofilm-based microalgal cultivation is a favoured approach to ADE treatment. This study compared Chlorella sp. MUR 268 and Scenedesmus sp. MUR 269 in biofilm and suspension cultures to treat anaerobic digestate food effluent (ADFE). Chlorella sp. MUR 268 biofilm had significantly higher biomass (50.38 g m-2) than Scenedesmus sp. biofilm (9.39 g m-2). Conversely, Scenedesmus sp. yielded 1.5 times more biomass (1.2 g L-1) than Chlorella sp. in suspension. Chlorella sp. biofilm had 49.3 % higher areal productivity than suspension, while Scenedesmus sp. showed 87.3 % higher areal growth in suspension. Chlorella sp. MUR 268 and Scenedesmus sp. MUR 269 significantly removed nutrients in ADFE. In suspension, COD, ammoniacal nitrogen, and phosphate were reduced to 94.9, 5.2, and 5.98 mg L-1 for Chlorella sp. MUR 268, and 245, 2.89, and 3.22 mg L-1 for Scenedesmus sp. MUR 269, respectively. In biofilm, Chlorella sp. MUR 268 achieved reductions to 149.9, 1.16, and 3.57 mg L-1, while Scenedesmus sp. MUR 269 achieved 100.2, 6.9 and 2.07 mg L-1. Most of these values are below the recommended effluent discharge standard, highlighting the efficacy of this system in ADFE treatment. Biofilm cultures fixed 68-81 % of removed nitrogen in biomass, while in suspension, only 55-71 % ended in the biomass. Chlorella sp. MUR 268 biofilm fixed 88 % of removed phosphorus, while Scenedesmus sp. MUR 269 suspension fixed more phosphorus (55 %) than the biofilm counterpart (34 %). This biofilm design offers advantages like simplified, cost-effective operation, easy biomass recovery, and reduced water usage.

Biofilm cultivation of chlorella species. MUR 269 to treat anaerobic digestate food effluent (ADFE): Total ammonia nitrogen (TAN) concentrations effect.

Microalgal-based treatment of anaerobic digestate food effluent (ADFE) has been found to be efficient and effective. However, turbidity and high total ammonia nitrogen (TAN)) content of ADFE is a major setback, requiring significant dilution. Although the possibility of growing microalgae in a high-strength ADFE with minimal dilution has been demonstrated in suspension cultures, such effluents remain highly turbid and affect the light path in suspension cultures. Here, the feasibility of growing Chlorella sp.MUR 269 in biofilm to treat ADFE with high TAN concentrations was investigated. Six different TAN concentrations in ADFE were evaluated for their effects on biofilm growth and nutrient removal by Chlorella sp. MUR 269 using the perfused biofilm technique. Biomass yields and productivities of this alga at various TAN concentrations (mg N NH3 L-1) were 55a (108 g m-2 and 9.80 g m-2 d-1)>100b > 200c = 300c = 500c > 1000d. Growth was inhibited, resulting in a 28% reduction in yield of Chlorella biofilm when this alga was grown at 1000 mg N NH3 L-1. A survey of the photosynthetic parameters reveals evidence of stress occurring in the following sequence: 55 < 100<200 < 300<1000. A significant nutrient removal was observed across various TAN concentrations. The removal pattern also followed the concentration gradients except COD, where the highest removal occurred at 500 mg N NH3 L-1. Higher removal rates were seen at higher nutrient concentrations and declined gradually over time. In general, our results indicated that the perfused biofilm strategy is efficient, minimizes water consumption, offers easy biomass harvesting, and better exposure to light. Therefore, it can be suitable for treating turbid and concentrated effluent with minimal treatment to reduce the TAN concentration.

Biomass and hydrocarbon production from Botryococcus braunii: A review focusing on cultivation methods.

Botryococcus braunii has garnered significant attention in recent years due to its ability to produce high amounts of renewable hydrocarbons through photosynthesis. As the world shifts towards a greener future and seeks alternative sources of energy, the cultivation of B. braunii and the extraction of its hydrocarbons can potentially provide a viable solution. However, the development of a sustainable and cost-effective process for cultivating B. braunii is not without challenges. Compared to other microalgae, B. braunii grows very slowly, making it time-consuming and expensive to produce biomass. In response to these challenges, several efforts have been put into optimizing Botryococcus braunii cultivation systems to increase biomass growth and hydrocarbon production efficiency. This review presents a comparative analysis of different Botryococcus braunii cultivation systems, and the factors affecting the productivity of biomass and hydrocarbon in Botryococcus braunii are critically discussed. Attached microalgal growth offers several advantages that hold significant potential for enhancing the economic viability of microalgal fuels. Here, we propose that employing attached growth cultivation, coupled with the milking technique for hydrocarbon extraction, represents an efficient approach for generating renewable fuels from B. braunii. Nevertheless, further research is needed to ascertain the viability of large-scale implementation.

Nano-clay modified membranes: A promising green strategy for microalgal antifouling filtration.

Membrane fouling is a major challenge which limits the sustainable application of membrane filtration-based microalgal harvesting at industrial level. Membrane fouling leads to increased operational and maintenance costs and represents a major obstacle to microalgal downstream processing. Nano-clays are promising naturally occurring nanoparticles in membrane fabrication due to their low-cost, facile preparation, and their superior properties in terms of surface hydrophilicity, mechanical stability, and resistance against chemicals. The membrane surface modification using nano-clays is a sustainable promising approach to improve membranes mechanical properties and their fouling resistance. However, the positive effects of nano-clay particles on membrane fouling are often limited by aggregation and poor adhesion to the base polymeric matrix. This review surveys the recent efforts to achieve anti-fouling behavior using membrane surface modification with nano-clay fillers. Further, strategies to achieve a better incorporation of nano-clay in the polymer matrix of the membrane are summarised, and the factors that govern the membrane fouling, stability, adhesion, agglomeration and leaching are discussed in depth.

Revolutionizing Pharmaceutical Industry: The Radical Impact of Artificial Intelligence and Machine Learning.

This article explores the significant impact of artificial intelligence (AI) and machine learning (ML) on the pharmaceutical industry, which has transformed the drug development process. AI and ML technologies provide powerful tools for analysis, decision-making, and prediction by simplifying complex procedures from drug design to formulation design. These techniques could potentially speed up the development of better medications and drug development processes, improving the lives of millions of people. However, the use of these techniques requires trained personnel and human surveillance for AI to function effectively, if not there is a possibility of errors like security breaches of personal data and bias can also occur. Thus, the present review article discusses the transformative power of AI and ML in the pharmaceutical industry and provides insights into the future of drug development and patient care.

Commercial paper as a promising carrier for biofilm cultivation of Chlorella sp. for the treatment of anaerobic digestate food effluent (ADFE): Effect on the photosynthetic efficiency.

Microalgal technology is still economically unattractive due to the high cost associated with microalgal cultivation and biomass recovery from conventional suspension cultures. Biofilm-based cultivation is a promising alternative for higher biomass yield and cheap/easy biomass harvesting opportunities. Additionally, using anaerobic digestate food effluent (ADFE) as a nutrient source reduces the cultivation cost and achieves ADFE treatment as an added value. However, the search for locally available, inexpensive, and efficient support materials is still open to research. This study evaluates the potential of commercially available, low-cost papers as support material for biofilm cultivation of Chlorella sp. and treatment of ADFE. Among the four papers screened for microalgal attachment, quill board paper performed better in higher biomass yield and stability throughout the study period. The attached growth study was done in a modular food container vessel, using anaerobic digestate food effluent (ADFE) as a nutrient source and a basal medium as a control. The microalgae grew well on the support material with higher biomass yield and productivity of 108.64 g(DW) m-2 and 9.96 g (DW) m-2 d-1, respectively, in the ADFE medium compared with 85.87 g (DW) m-2 and 4.99 g (DW) m-2 d-1, respectively in the basal medium. Chlorophyll, a fluorescence (ChlF) probe, showed that cell density in the biofilm significantly changes the photosynthetic apparatus of the algae, with evidence of stress observed as the culture progressed. Also, efficient nutrient removal from the ADFE medium was achieved in the 100 %, 85 %, and 40.2 % ratios for ammoniacal nitrogen, phosphate, and chemical oxygen demand (COD). Therefore, using quill board paper as carrier material for microalgal cultivation offers promising advantages, including high biomass production, easy biomass harvesting (by scrapping or rolling the biomass with the paper), and efficient effluent treatment.

Genotoxic and mutagenic potential of 7-methylxanthine: an investigational drug molecule for the treatment of myopia.

7-Methylxanthine (7-MX, CAS No. 552-62-5, purity 99.46%) is the first orally administered drug candidate, which showed anti-myopic activity in different pre-clinical studies. In the present study, we investigated the in-vivo genotoxic and mutagenic toxicity of 7-MX in Wistar rats using comet/single-cell gel electrophoresis, chromosomal aberration and micronucleus assays after oral administration. For the single-dose study (72 h), two doses of 7-MX 300 and 2000 mg/kg body weight were selected. For a repeated dose 28 d study, three doses (250, 500, and 1000 mg/kg) of 7-MX were selected. The doses were administered via oral gavage in the suspension form. Blood and major vital organs such as bone marrow, lung and liver were used to perform comet/single cell gel electrophoresis, chromosomal aberration, and micronucleus assays. The in-vitro Ames test was performed on TA98 and TA100 strains. In the chromosomal aberration study, a non-significant increase in deformities such as stickiness, ring chromosome, and endoreduplication was observed in bone marrow cells of 7-MX treated groups. These chromosomal alterations were observed upon treatment with doses of 2000 mg/kg single dose for 72 h and 1000 mg/kg repeated dose for 28 d. At a dose of 500 mg/kg, DNA damage in terms of tail length, tail moment, % tail DNA and the olive tail moment was also found to be non-significant in 7-MX treated groups. The Ames test showed the non-mutagenic nature of 7-MX in both strains of TA98 and TA100 of Salmonella typhimurium with or without metabolic activation. Thus, the present work is interesting in view of the non- genotoxicity and non-mutagenicity of repeated doses of 7-MX.

Graphene-based catalysts for biodiesel production: Characteristics and performance.

Biodiesel is a promising alternative to reduce the dependency on fossil fuels. However, biodiesel's cost is still higher than its petroleum counterpart, hence its production process must be modified to make it economically viable. Microalgae are an alternative feedstock to replace agricultural crops for biodiesel production, and offer several advantages such as fast growth, use of non-arable land, growth in saline and wastewater, and high lipid yield. Unfortunately, biodiesel production from microalgae is very energy-intensive and costly, mainly due to the high energy consumption required for dewatering and drying. Therefore, utilizing wet microalgal biomass instead of dry biomass can be a promising solution to reduce the biodiesel production cost Furthermore, the use of heterogeneous catalysts offers high efficiency, recoverability, and reusability, and is therefore very promising from the economic and environmental perspectives. The unique characteristics of graphene-based nano-catalysts, such as their high surface area, two-dimensional structure, and functional groups, make them suitable candidates for biodiesel production. In this review, the use of graphene-based catalysts for biodiesel production is analyzed in depth, and their efficiency compared to other heterogeneous catalysts is scrutinized. Moreover, their recoverability, reusability, and economic feasibility are critically discussed, and their potential to produce biodiesel from wet microalgae is explored as a sustainable and cost-effective approach.

Myopia, its prevalence, current therapeutic strategy and recent developments: A Review.

Myopia is a widespread and complex refractive error in which a person's ability to see distant objects clearly is impaired. Its prevalence rate is increasing worldwide, and as per WHO, it is projected to increase from 22% in 2000 to 52% by 2050. It is more prevalent in developed, industrial areas and affects individuals of all ages. There are a number of treatments available for the control of myopia, such as glasses, contact lenses, laser surgery, and pharmaceuticals agents. However, these treatments are less beneficial and have significant side effects. A novel molecule, 7-methylxanthine (7-MX), has been found to be a highly beneficial alternate in the treatment of myopia and excessive eye elongation. Many preclinical and clinical studies showed that 7-MX is effective for the treatment of myopia and is presently under phase II of clinical investigation. We have also investigated preclinical toxicity studies such as acute, sub-acute, sub-chronic, and chronic on rats. In these studies, 7-MX was found to be non-toxic as compared to other reported anti-myopic agents. Moreover, as an ideal drug, 7-MX is observed to have no or low toxicity, brain permeability, non-allergic, higher oral administration efficacy, and low treatment costs and thus qualifies for the long-term treatment of myopia. This review article on 7-MX as an alternative to myopia treatment will highlight recent findings from well-designed preclinical and clinical trials and propose a potential future therapy.

Novel Self-micro Emulsifying Drug Delivery System for Safe Intramuscular Delivery with Improved Pharmacodynamics and Pharmacokinetics.

BACKGROUND: Diclofenac Sodium (DS) injection is widely used in the management of acute or chronic pain and inflammatory diseases. It incorporates 20% w/v Transcutol-P as a solubilizer to make the stable injectable formulation. However, the use of Transcutol-P in high concentration leads to adverse effects such as severe nephrotoxicity, etc. Some advancements have resulted in the formulation of an aqueous-based injectable but that too used benzyl alcohol which is reported to be toxic for human use. OBJECTIVE: This study aimed to develop an injectable Self-Micro Emulsifying Drug Delivery System (SMEDDS) as a novel carrier of DS for prompt release with better safety and efficacy. METHODS: A solubility study was performed with different surfactants and co-surfactants. The conventional stirring method was employed for the formulation of SMEDDS. Detailed in vitro characterization was done for different quality control parameters. In vivo studies were performed using Wistar rats for pharmacokinetic evaluation, toxicological analysis, and analgesic activity. RESULTS: The optimized formulation exhibited good physical stability, ideal globule size (156±0.4 nm), quick release, better therapeutics, and safety, increase in LD50 (221.9 mg/kg) to that of the commercial counterpart (109.9 mg/kg). Furthermore, pre-treatment with optimized formulation reduced the carrageenan-induced rat paw oedema by 88±1.2% after 4 h, compared to 77±1.6% inhibition with commercial DS formulation. Moreover, optimized formulation significantly (p<0.05) inhibited the pain sensation in the acetic-acid induced writhing test in mice compared to its commercial equivalent with a better pharmacokinetic profile. CONCLUSION: The above findings confirmed that liquid SMEDDS can be a successful carrier for the safe and effective delivery of DS.

Olive oil and oleic acid-based self nano-emulsifying formulation of omega-3-fatty acids with improved strength, stability, and therapeutics.

AIM: To develop, characterise, and optimise SNEDDS formulation to enhance organoleptics, bioavailability, physical & oxidative-stability, and extend shelf-life of pure Ω-3-fatty acids oil for use in the food fortification industry as nutraceuticals. METHODS: SNEDDS formulations were prepared using a simple stirring technique and optimised based on in-vitro characterisation. RESULTS: The optimised SNEDDS formulation (F3) had a mean diameter of 52.9 ± 0.4 nm, PDI of 0.229 ± 0.02, zeta potential of -17.3 ± 0.1 mV, cloud temperature of 92 ± 0.2 °C, self-emulsification time of 50 ± 0.2 sec, and stable under accelerated stability conditions. Intestinal permeability study on rat ileum depicted absorption of 88.5 ± 0.2% DHA at 5 h for F3 formulation in comparison to 61.5 ± 0.2% for commercial counterpart. F3 formulation exhibited better therapeutics for melamine-induced cognitive dysfunction. CONCLUSIONS: The developed Ω-3-loaded SNEDDS heralds the future for an efficacious, safer, and higher strength formulation intended as a better substitute for currently available formulations.

Nonconvulsive status epilepticus: overlooked and undertreated.

Nonconvulsive status epilepticus (NCSE) is characterized by persistent change in mental status from baseline lasting more than 5 minutes, generally with epileptiform activity seen on EEG monitoring and subtle or no motor abnormalities. NCSE can be a difficult diagnosis to make in the emergency department setting, but the key to diagnosis is a high index of suspicion coupled with rapid initiation of continuous EEG and early involvement of neurology. Benzodiazepines are the mainstay of first-line therapy, with antiepileptic drugs and anesthetics as second- and third-line therapies, respectively. The few established guidelines on the treatment of NCSE are highly variable, and the objective of this comprehensive review is to create a standardized evidence-based protocol for the diagnosis and treatment of NCSE.

Red and blue luminescent solar concentrators for increasing Arthrospira platensis biomass and phycocyanin productivity in outdoor raceway ponds.

Achieving high biomass productivity is critical for establishing a successful large-scale algal facility. Microalgae cultures in raceway ponds are normally light limited. To achieve high biomass productivity, there is a need to develop a system to deliver light into the depth of microalgal cultures in raceway ponds. We investigated red and blue luminescent solar concentrators (LSCs) in outdoor raceway ponds to downgrade the sunlight, re-emit and, deliver it into the depth of Arthrospira platensis culture operated at 21 cm depth. When red LSCs were used, the biomass productivity (12.2 g m-2 d-1) and phycocyanin productivity (8.5 mg L-1 d-1) of A. platensis increased 26% and 44%, respectively. However, using blue LSCs resulted in no significant increase in A. platensis biomass productivity. Therefore, for generating same phycocyanin productivity using red LSCs, 44% less cultivation area would be required. This can lead to a significant reduction in the cost of phycocyanin production.

Viability of combining microalgae and macroalgae cultures for treating anaerobically digested piggery effluent.

Algal phytoremediation represents a practical green solution for treating anaerobically digested piggery effluent (ADPE). The potential and viability of combining microalgae and macroalgae cultivation for the efficient treatment of ADPE were evaluated in this study. Bioprospecting the ability of different locally isolated macroalgae species illustrated the potential of Cladophora sp. to successfully grow and treat ADPE with up to 150 mg/L NH4+ with a biomass productivity of (0.13 ±â€¯0.02) g/(L·day) and ammonium removal rate of (10.23 ±â€¯0.18) mg/(L·day) NH4+. When grown by itself, the microalgae consortium used in this study consisting of Chlorella sp. and Scenedesmus sp. was found to grow and treat undiluted ADPE (up to 525 mg/L NH4+) with an average ammonium removal rate of 25 mg/(L·day) NH4+ and biomass productivity of (0.012 ±â€¯0.0001) g/(L·day). Nevertheless, when combined together, despite the different cultivation systems (attached and non-attached) evaluated, microalgae and macroalgae were unable to co-exist together and treat ADPE as their respective growth were inversely related to each other due to direct competition for nutrients and available resources as well as the negative physical interaction between both algal groups.

Sliding mode current control of a NOCULL converter based on hysteresis modulation method in a wide range of operating conditions.

This study presents a novel control scheme for voltage regulation of a negative output cascade ultra-lift Luo converter (NOCULLC). Due to the complicated high order model of the converter, its performance is degraded against large load variations, input voltage changes and parametric uncertainties. In order to enhance the converter behavior, a PI compensator and a sliding mode current controller are combined to control the fourth order NOCULLC worked in a wide range of operating conditions. Because of the non-minimum phase structure of the converter, the output voltage of the converter is indirectly regulated by enforcing the input inductor current to track its reference signal. The sliding mode current controller improves the dynamic and static performances of the converter by minimizing the inrush current of the input inductor of the converter and also, the PI controller eliminates the steady state error of the output voltage of the converter. The closed loop system stability is demonstrated by using sliding mode theory. Furthermore, this paper proposes a systematic procedure to compute the gains of the controller based on the stability constraints which guarantees robustness of the developed controller. The experimental results show the robustness and effectiveness of the proposed controller against parametric variations and uncertainties of the converter. Also, a comprehensive comparison of the general aspects of the properties between the developed method and other existed controllers is provided.

Hemodynamic Changes in Experimentally Envenomed Anaesthetized Rats by Intravenous Injection of Hemiscorpius lepturus Venom.

BACKGROUND: We investigated the hemodynamic changes (Inotropic, chronotropic and arrhythmogenic) in intravenously envenomed anesthetized rats with Hemiscorpius lepturus venom. The neutralizing potencies of different drugs and commercial antivenom were assessed simultaneously. METHODS: Different doses of the crude venom (100, 200 and 400µg/rat) were injected during five minutes via the femoral vein and cardiovascular changes were recorded in rats in Razi Institute Corporation, Karaj, Iran in 2017. The drugs (Atropine, lidocaine, propranolol and prazosin) were injected before the venom for determination of the counteracting effects. Different volumes (100, 500 and 1000µl) of the antivenom were pre envenomed to neutralize cardiovascular changes. RESULTS: Temporary hypertension and bradycardia with no arrhythmogenic effects were depicted within twenty minutes. There was a difference in arterial pressure between the venom (400µg/rat) and the vehicle at 8 minutes (114.68±5.1mmHg versus 70.2±4.3mmHg). Elevation of the mean arterial pressure was inhibited by propranolol (2 mg/kg) and neutralized by prazosin (1mg/kg) while lidocaine (4mg/kg) and atropine (1mg/kg) had no effects. Premedication with Iranian commercial antivenom (1000µl) produced surprisingly temporary hypertension compared to the vehicle (140.84±4.5 versus 84.3±3.2). It had no neutralizing properties on blood pressure variation before the venom injection. Volume-expanded hypertension phenomenon was ruled out in a parallel study. CONCLUSION: This venom has vasoconstrictive effects in rats probably due to the presence of norepinephrine like materials in its content or liberated from adrenal gland inhibited by prazosin premedication. The neutralizing effects of antivenom on venom-induced hypertension are questionable.

Macroalgae culture to treat anaerobic digestion piggery effluent (ADPE).

Environmental consequences of high productivity piggeries are significant and can result in negative environmental impacts, hence bioremediation techniques (in particular using macroalgae) are therefore of great interest. Here, the growth potential of several freshwater macroalgae in anaerobic digestion piggery effluent (ADPE), their nutrient removal rates and biochemical composition of the biomass were investigated under outdoor climatic conditions. A consortium of two macroalgae, Rhizoclonium sp. and Ulothrix sp. was isolated and could efficiently grow in the ADPE. Maximum ammonium removal rate (30.6±6.50mg NH4+-NL-1d-1) was achieved at ADPE concentration equivalent to 248mgNH4+-NL-1. Mean biomass productivity of 31.1±1.14g ash-free dry weight (AFDW) m-2d-1 was achieved. Total carbohydrate and protein contents ranged between 42.8-54.8 and 43.4-45.0% AFDW, respectively, while total lipid content was very low. The study indicates the potential use of this macroalgal consortium for treating ADPE as well as source of animal feed production.