Habitat suitability, range dynamics, and threat assessment of Swertia petiolata D. Don: a Himalayan endemic medicinally important plant under climate change.

In the current era of the anthropocene, climate change is one of the main determinants of species redistribution and biodiversity loss. Worryingly, the situation is alarming for endemic and medicinally important plant species with a narrow distributional range. Therefore, it is pivotal to inspect the influence of accelerated climate change on medicinally important threatened and endemic plant species. Using an ensemble approach, the current study aims at modelling the present distribution and predicting the future potential distribution coupled with the threat assessment of Swertia petiolata-a medicinally important endemic plant species in the Himalayan biodiversity hotspot. Our study revealed that under current climatic scenarios, the suitable habitats for the species occur across the western Himalayan region which includes the north-western Indian states (Jammu and Kashmir, Himachal Pradesh, and southern Uttarakhand), northern Pakistan, and north-western Nepal. Also, temperature seasonality (BIO4) and precipitation seasonality (BIO15) are the most significant bioclimatic variables determining the distribution of S. petiolata. Furthermore, the study projected a reduction in the suitable habitats for the species under future changing climatic scenarios with a reduction ranging from - 40.298% under RCP4.5 2050 to - 83.421% under RCP8.5 2070. Most of the habitat reduction will occur in the western Himalayan region. In contrast, some of the currently unsuitable Himalayan regions like northern Uttarakhand will show increasing suitability under climate change scenarios. The current study also revealed that S. petiolata is classified as Near Threatened (NT) following the IUCN criterion B. Hopefully, the present study will provide a robust tool for predicting the cultivation hotspots and devising scientifically effective conservation strategies for this medicinally important plant species in the Himalaya and similar environments elsewhere in the world.

Effect of plant growth regulators on in vitro induction and maintenance of callus from leaf and root explants of Atropa acuminata Royal ex Lindl.

Atropa acuminata, an important medicinal plant belonging to family Solanaceae is under tremendous threat of extinction in its natural habitat due to the overexploitation by pharmaceutical industries. Present study is an attempt of establishing callus cultures of this important medicinal plant as callus has considerable potential as an alternative for production of secondary metabolites for industrial use, hence reducing pressure on natural populations. Callus cultures were established from leaf and root explants of Atropa acuminate. Murashige and Skoog (MS) media containing different concentration and combinations of 6-Benzyl Amino Purine (BAP), Naphthalene Acetic Acid (NAA), Kinetin (Kn) and 2,4- Dichloropheoxyacetic acid (2,4- D) were used for callus induction. Different phytohormonal combinations resulted in different types and degrees of callus. The combination of BAP and NAA on MS media supplemented with 0.5 mg/l BAP in combination with 1.0 mg/l NAA, was found to be the most efficient for in vitro callus development from root explants and from leaf explants most effective combination and concentration was 1 mg/l of both BAP and NAA. The maximum mean fresh weight of callus formed using root explants was 33.13 mg per explant and maximum fresh weight obtained from leaf explants was 22.14 mg per explants.

Anticoccidial activity of fruit peel of Punica granatum L.

In the interests of food safety and public health, plants and their compounds are now re-emerging as an alternative approach to treat parasitic diseases. Here, we studied the anticoccidial effect of different solvent extracts of the fruit peel of Punica granatum-a commercial waste from pomegranate juice industries. The hope underlying these experiments was to find a sustainable natural product for controlling coccidiosis. The plant extracts were prepared using solvents of different polarity. Acute oral toxicity study was first carried out to see the safety of crude extracts. A high dose of crude extracts (300 mg/kg body weight) was tested for possession of anticoccidial activity against experimentally induced coccidial infection in broiler chicken. Activity was measured in comparison to the reference drug amprolium on the basis of oocyst output reduction, mean weight gain of birds and feed conversion ratio. Oocyst output was measured using Mc-Masters counting technique. Acute oral toxicity study showed that crude extracts of P. granatum are safe up to dosage of 2000 mg/kg body weight. LD50 was not determined as mortalities were not recorded in any of the five groups of chicken. For anticoccidial activity crude methanolic extract (CME) of the fruit peel of P. granatum showed the maximum effect as evident by oocyst output reduction (92.8 ±â€¯15.3), weight gain of birds (1403.0 ±â€¯11.9 g) and feed conversion ratio (1.66 ±â€¯0.04), thereby affirming the presence of alcohol soluble active ingredients in the plant. We also tested different doses (100-400 mg/kg body weight) of the CME of the fruit peel of P. granatum, the most active extract on E. tenella and observed a dose dependent effect. From the present study it can be concluded that alcoholic extract of the fruit peel of P. granatum has significant potential to contribute to the control of coccidian parasites of chicken.

Anticoccidial activity of Artemisia vestita (Anthemideae, Asteraceae) - a traditional herb growing in the Western Himalayas, Kashmir, India.

Coccidiosis, caused by various species of genus Eimeria, is a major parasitic disease in chicken. The increasing resistance of these parasites to currently used anticoccidial drugs has stimulated the search for new methods of control. As part of this effort, a study was designed to see the anticoccidial effect of different solvent extracts of Artemisia vestita-a traditional herb growing in Kashmir Himalayas. The plant extracts were prepared using different solvents. Preliminary toxicity study was first carried out to see the safety of crude plant extracts. A high dose of crude extracts (300 mg/kg body weight) was tested for possession of anticoccidial activity against experimentally induced coccidial infection in broiler chicken. Activity was measured in comparison to the reference drug amprolium on the basis of oocyst output reduction, mean weight gain of birds and feed conversion ratio. Oocyst output was measured using Mc-Masters counting technique. Preliminary toxicity study showed that crude extracts of A. vestita are safe up to dosage of 2000 mg/kg body weight. LD50 was not determined as mortalities were not recorded in any of the five groups of chicken. For anticoccidial activity crude methanolic extract (CME) of A. vestita showed the maximum effect as evident by oocyst output reduction (71.5 ± 12.2), weight gain of birds (1406.4 ± 12.2) and feed conversion ratio (1.58 ± 0.06), thereby affirming the presence of alcohol soluble active ingredients in the plant. We also tested different doses (100-400 mg/kg body weight) of the CME of A. vestita, the most active extract on E. tenella and observed a dose dependent effect. From the present study it can be concluded that alcoholic extract of A. vestita has the immense potential to contribute to the control of coccidian parasites of chicken. Our results corroborate the use of genus Artemisia and could justify its use in folk medicine for treatment of parasitic diseases.