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1.
Cell ; 154(6): 1300-13, 2013 Sep 12.
Article in English | MEDLINE | ID: mdl-24034252

ABSTRACT

Radial glial progenitors (RGPs) are elongated epithelial cells that give rise to neurons, glia, and adult stem cells during brain development. RGP nuclei migrate basally during G1, apically using cytoplasmic dynein during G2, and undergo mitosis at the ventricular surface. By live imaging of in utero electroporated rat brain, we find that two distinct G2-specific mechanisms for dynein nuclear pore recruitment are essential for apical nuclear migration. The "RanBP2-BicD2" and "Nup133-CENP-F" pathways act sequentially, with Nup133 or CENP-F RNAi arresting nuclei close to the ventricular surface in a premitotic state. Forced targeting of dynein to the nuclear envelope rescues nuclear migration and cell-cycle progression, demonstrating that apical nuclear migration is not simply correlated with cell-cycle progression from G2 to mitosis, but rather, is a required event. These results reveal that cell-cycle control of apical nuclear migration occurs by motor protein recruitment and identify a role for nucleus- and centrosome-associated forces in mitotic entry. PAPERCLIP:


Subject(s)
Brain/embryology , Cell Nucleus/metabolism , Dyneins/metabolism , Mitosis , Neural Stem Cells/cytology , Nuclear Pore/metabolism , Animals , Brain/cytology , Carrier Proteins/metabolism , Centrosome/metabolism , Embryo, Mammalian/metabolism , Membrane Proteins/metabolism , Microtubule-Associated Proteins , Neural Stem Cells/metabolism , Neurogenesis , Rats
2.
J Cell Biol ; 192(5): 855-71, 2011 Mar 07.
Article in English | MEDLINE | ID: mdl-21383080

ABSTRACT

Centrosomes are closely associated with the nuclear envelope (NE) throughout the cell cycle and this association is maintained in prophase when they separate to establish the future mitotic spindle. At this stage, the kinetochore constituents CENP-F, NudE, NudEL, dynein, and dynactin accumulate at the NE. We demonstrate here that the N-terminal domain of the nuclear pore complex (NPC) protein Nup133, although largely dispensable for NPC assembly, is required for efficient anchoring of the dynein/dynactin complex to the NE in prophase. Nup133 exerts this function through an interaction network via CENP-F and NudE/EL. We show that this molecular chain is critical for maintaining centrosome association with the NE at mitotic entry and contributes to this process without interfering with the previously described RanBP2-BICD2-dependent pathway of centrosome anchoring. Finally, our study reveals that tethering of centrosomes to the nuclear surface at the G2/M transition contributes, along with other cellular mechanisms, to early stages of bipolar spindle assembly.


Subject(s)
Centrosome/metabolism , Nuclear Envelope/metabolism , Nuclear Pore Complex Proteins/physiology , Nuclear Pore/metabolism , Prophase , Carrier Proteins/metabolism , Carrier Proteins/physiology , Cell Line, Tumor , Cell Polarity , Centrosome/ultrastructure , Chromosomal Proteins, Non-Histone/metabolism , Chromosomal Proteins, Non-Histone/physiology , Dynactin Complex , Dyneins/metabolism , HeLa Cells , Humans , Intranuclear Space/metabolism , Intranuclear Space/ultrastructure , Microfilament Proteins/metabolism , Microfilament Proteins/physiology , Microtubule-Associated Proteins/metabolism , Minor Histocompatibility Antigens , Nuclear Envelope/ultrastructure , Nuclear Pore Complex Proteins/chemistry , Nuclear Pore Complex Proteins/metabolism , Protein Interaction Mapping , Spindle Apparatus/metabolism
3.
J Cell Biol ; 190(3): 317-30, 2010 Aug 09.
Article in English | MEDLINE | ID: mdl-20679430

ABSTRACT

A cold-sensitive gamma-tubulin allele of Aspergillus nidulans, mipAD159, causes defects in mitotic and cell cycle regulation at restrictive temperatures that are apparently independent of microtubule nucleation defects. Time-lapse microscopy of fluorescently tagged mitotic regulatory proteins reveals that cyclin B, cyclin-dependent kinase 1, and the Ancdc14 phosphatase fail to accumulate in a subset of nuclei at restrictive temperatures. These nuclei are permanently removed from the cell cycle, whereas other nuclei, in the same multinucleate cell, cycle normally, accumulating and degrading these proteins. After each mitosis, additional daughter nuclei fail to accumulate these proteins, resulting in an increase in noncycling nuclei over time and consequent inhibition of growth. Extensive analyses reveal that these noncycling nuclei result from a nuclear autonomous, microtubule-independent failure of inactivation of the anaphase-promoting complex/cyclosome. Thus, gamma-tubulin functions to regulate this key mitotic and cell cycle regulatory complex.


Subject(s)
Aspergillus nidulans/metabolism , Tubulin/metabolism , Ubiquitin-Protein Ligase Complexes/metabolism , Alleles , Anaphase-Promoting Complex-Cyclosome , CDC2 Protein Kinase/metabolism , Cell Cycle , Cyclin B/metabolism , Mitosis , Mutation , Polymerase Chain Reaction , Recombinant Fusion Proteins/genetics , Tubulin/genetics
4.
Mol Biol Cell ; 20(12): 2874-84, 2009 Jun.
Article in English | MEDLINE | ID: mdl-19386763

ABSTRACT

In the filamentous fungus Aspergillus nidulans, cytokinesis/septation is triggered by the septation initiation network (SIN), which first appears at the spindle pole body (SPB) during mitosis. The coiled-coil protein SNAD is associated with the SPB and is required for timely septation and conidiation. We have determined that SNAD acted as a scaffold protein that is required for the localization of the SIN proteins of SIDB and MOBA to the SPB. Another scaffold protein SEPK, whose localization at the SPB was dependent on SNAD, was also required for SIDB and MOBA localization to the SPB. In the absence of either SEPK or SNAD, SIDB/MOBA successfully localized to the septation site, indicating that their earlier localization at SPB was not essential for their later appearance at the division site. Unlike their functional counterparts in fission yeast, SEPK and SNAD were not required for vegetative growth but only for timely septation. Furthermore, down-regulation of negative regulators of the SIN suppressed the septation and conidiation phenotypes due to the loss of SNAD. Therefore, we conclude that SPB localization of SIN components is not essential for septation per se, but critical for septation to take place in a timely manner in A. nidulans.


Subject(s)
Aspergillus nidulans/cytology , Aspergillus nidulans/metabolism , Cell Cycle Proteins/metabolism , Cytokinesis , Fungal Proteins/metabolism , Spindle Apparatus/metabolism , Actomyosin/metabolism , Down-Regulation , GTPase-Activating Proteins/metabolism , Mutation/genetics , Protein Transport , Sequence Homology, Amino Acid , Time Factors
5.
Mol Biol Cell ; 20(8): 2146-59, 2009 Apr.
Article in English | MEDLINE | ID: mdl-19225157

ABSTRACT

During open mitosis several nuclear pore complex (NPC) proteins have mitotic specific localizations and functions. We find that the Aspergillus nidulans Mlp1 NPC protein has previously unrealized mitotic roles involving spatial regulation of spindle assembly checkpoint (SAC) proteins. In interphase, An-Mlp1 tethers the An-Mad1 and An-Mad2 SAC proteins to NPCs. During a normal mitosis, An-Mlp1, An-Mad1, and An-Mad2 localize similarly on, and around, kinetochores until telophase when they transiently localize near the spindle but not at kinetochores. During SAC activation, An-Mlp1 remains associated with kinetochores in a manner similar to An-Mad1 and An-Mad2. Although An-Mlp1 is not required for An-Mad1 kinetochore localization during early mitosis, it is essential to maintain An-Mad1 in the extended region around kinetochores in early mitosis and near the spindle in telophase. Our data are consistent with An-Mlp1 being part of a mitotic spindle matrix similar to its Drosophila orthologue and demonstrate that this matrix localizes SAC proteins. By maintaining SAC proteins near the mitotic apparatus, An-Mlp1 may help monitor mitotic progression and coordinate efficient mitotic exit. Consistent with this possibility, An-Mad1 and An-Mlp1 redistribute from the telophase matrix and associate with segregated kinetochores when mitotic exit is prevented by expression of nondegradable cyclin B.


Subject(s)
Aspergillus nidulans/cytology , Aspergillus nidulans/metabolism , Fungal Proteins/metabolism , Mitosis , Spindle Apparatus/metabolism , Cyclin B/metabolism , Kinetochores/metabolism , Nuclear Pore/metabolism , Protein Binding , Protein Processing, Post-Translational , Protein Transport , Telophase , Time Factors
6.
Chem Biol ; 15(6): 527-32, 2008 Jun.
Article in English | MEDLINE | ID: mdl-18559263

ABSTRACT

The recently sequenced genomes of several Aspergillus species have revealed that these organisms have the potential to produce a surprisingly large range of natural products, many of which are currently unknown. We have found that A. nidulans produces emericellamide A, an antibiotic compound of mixed origins with polyketide and amino acid building blocks. Additionally, we describe the discovery of four previously unidentified, related compounds that we designate emericellamide C-F. Using recently developed gene targeting techniques, we have identified the genes involved in emericellamide biosynthesis. The emericellamide gene cluster contains one polyketide synthase and one nonribosomal peptide synthetase. From the sequences of the genes, we are able to deduce a biosynthetic pathway for the emericellamides. The identification of this biosynthetic pathway opens the door to engineering novel analogs of this structurally complex metabolite.


Subject(s)
Aspergillus nidulans/metabolism , Depsipeptides/biosynthesis , Information Storage and Retrieval , Aspergillus nidulans/genetics , Fermentation , Gene Targeting , Genes, Fungal , Mass Spectrometry , Open Reading Frames
7.
Genetics ; 172(3): 1557-66, 2006 Mar.
Article in English | MEDLINE | ID: mdl-16387870

ABSTRACT

Aspergillus nidulans is an important experimental organism, and it is a model organism for the genus Aspergillus that includes serious pathogens as well as commercially important organisms. Gene targeting by homologous recombination during transformation is possible in A. nidulans, but the frequency of correct gene targeting is variable and often low. We have identified the A. nidulans homolog (nkuA) of the human KU70 gene that is essential for nonhomologous end joining of DNA in double-strand break repair. Deletion of nkuA (nkuA delta) greatly reduces the frequency of nonhomologous integration of transforming DNA fragments, leading to dramatically improved gene targeting. We have also developed heterologous markers that are selectable in A. nidulans but do not direct integration at any site in the A. nidulans genome. In combination, nkuA delta and the heterologous selectable markers make up a very efficient gene-targeting system. In experiments involving scores of genes, 90% or more of the transformants carried a single insertion of the transforming DNA at the correct site. The system works with linear and circular transforming molecules and it works for tagging genes with fluorescent moieties, replacing genes, and replacing promoters. This system is efficient enough to make genomewide gene-targeting projects feasible.


Subject(s)
Aspergillus nidulans/genetics , Gene Targeting/methods , Antigens, Nuclear/genetics , Aspergillus fumigatus/genetics , DNA-Binding Proteins/genetics , Genetic Markers , Humans , Ku Autoantigen , Mutation , Plasmids , Sequence Homology, Nucleic Acid
8.
Nat Protoc ; 1(6): 3111-20, 2006.
Article in English | MEDLINE | ID: mdl-17406574

ABSTRACT

We describe a rapid method for the production of fusion PCR products that can be used, generally without band purification, to transform Aspergillus nidulans. This technique can be used to replace genes; tag genes with fluorescent moeties or epitope tags; or replace endogenous promoters with regulatable promoters, by introducing an appropriate selective cassette (e.g., fluorescent protein + selectable marker). The relevant genomic fragments and cassette are first amplified separately by PCR using primers that produce overlapping ends. A second PCR using 'nested' primers fuses the fragments into a single molecule with all sequences in the desired order. This procedure allows a cassette to be amplified once, frozen and used subsequently in many fusion PCRs. Transformation of nonhomologous recombination deficient (nkuADelta) strains of A. nidulans with fusion PCR products results in high frequencies of accurate gene targeting. Fusion PCR takes less than 2 d. Protoplast formation and transformation takes less than 1 d.


Subject(s)
Aspergillus nidulans/genetics , Gene Targeting/methods , Polymerase Chain Reaction/methods , Gene Transfer Techniques , Protoplasts
9.
Mol Biol Cell ; 15(3): 1374-86, 2004 Mar.
Article in English | MEDLINE | ID: mdl-14668489

ABSTRACT

Recent data from multiple organisms indicate that gamma-tubulin has essential, but incompletely defined, functions in addition to nucleating microtubule assembly. To investigate these functions, we examined the phenotype of mipAD159, a cold-sensitive allele of the gamma-tubulin gene of Aspergillus nidulans. Immunofluorescence microscopy of synchronized material revealed that at a restrictive temperature mipAD159 does not inhibit mitotic spindle formation. Anaphase A was inhibited in many nuclei, however, and after a slight delay in mitosis (approximately 6% of the cell cycle period), most nuclei reentered interphase without dividing. In vivo observations of chromosomes at a restrictive temperature revealed that mipAD159 caused a failure of the coordination of late mitotic events (anaphase A, anaphase B, and chromosomal disjunction) and nuclei reentered interphase quickly even though mitosis was not completed successfully. Time-lapse microscopy also revealed that transient mitotic spindle abnormalities, in particular bent spindles, were more prevalent in mipAD159 strains than in controls. In experiments in which microtubules were depolymerized with benomyl, mipAD159 nuclei exited mitosis significantly more quickly (as judged by chromosomal condensation) than nuclei in a control strain. These data reveal that gamma-tubulin has an essential role in the coordination of late mitotic events, and a microtubule-independent function in mitotic checkpoint control.


Subject(s)
Aspergillus nidulans/metabolism , Cell Nucleus/metabolism , Microtubules/metabolism , Spindle Apparatus/metabolism , Tubulin/metabolism , Cell Cycle/physiology , Microscopy, Fluorescence , Mutation/genetics
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